长白山野生松针粉抗氧化及抗衰老作用的研究
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摘要
就长白山野生松针粉对老年小鼠抗氧化作用以及果蝇延缓衰老作用进行研究。选取美国癌症研究所(Institute of Cancer Research,ICR)老龄小鼠20只随机分为老年模型组与老年给药组,同样选取10只正常年龄的ICR小鼠为青年对照组,老年给药组给予剂量为5 g/kg长白山野生松针粉30天后,检测各组血清中丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)、肝组织中谷胱甘肽(glutathion,GSH)等氧化指标。同时以果蝇为生物模式,在其培养基中添加低(0.115%)、中(0.461%)、高(1.845%)不同浓度的松针粉,对果蝇寿命相关行为学指标进行考察。结果表明:与老年模型组相比,松针粉可显著性增加老年给药组小鼠血清SOD(P<0.05)、肝组织GSH含量(P<0.05),降低MDA含量。松针粉可延长果蝇寿命,增加果蝇的子代数量,提高老年蝇的攀爬能力,提高老龄果蝇抗亚急性氧化损伤能力及体内水份含量。长白山野生松针粉具有抗氧化及抗衰老作用,可改善老年小鼠、果蝇与寿命相关的指标。
It intended to research that wild pine needle powder in Changbai Mountain might play a role on antioxidation to the aging mice and anti-aging to the drosophilas. 20 Institute of Cancer Research(ICR)mice were divided into the elderly model group and the elderly medication group randomly and averagely,10 ICR mice of normal age were also selected as the young control group. The serum malondialdehyde(MDA),superoxide dismutase(SOD)and glutathione(GSH)in liver tissue and other oxidation indices were detected for each group after giving wild pine needle powder in Changbai Mountain for dose of 5 g/kg to the the elderly medication group in 30 days. Meanwhile,drosophilas,as biological model,were given the lower(0.115 %),the medium(0.461 %),and the higher(1.845 %)concentration of pine needle powder added to the culture mediums and then observed the life related behavioral indexes of drosophilas. Compared with elderly model group,it showed that the pine needle powder could not only significantly increase the content of serum SOD(P<0.05)and GSH in liver tissue(P<0.05)but reduce the MDA in the elderly medication group. The results also showed that pine needle powder could prolong the lifespan,increase the offspring numbers,enhance the climbing ability and the anti-sub-acute oxidative damage capacity and body water content of the aging drosophilas. The wild pine needle powder in Changbai Mountain obtained antioxidant and anti-aging effects which could improve the liferelated indicators of aging mice and drosophilas.
It intended to research that wild pine needle powder in Changbai Mountain might play a role on antioxidation to the aging mice and anti-aging to the drosophilas. 20 Institute of Cancer Research(ICR)mice were divided into the elderly model group and the elderly medication group randomly and averagely,10 ICR mice of normal age were also selected as the young control group. The serum malondialdehyde(MDA),superoxide dismutase(SOD)and glutathione(GSH)in liver tissue and other oxidation indices were detected for each group after giving wild pine needle powder in Changbai Mountain for dose of 5 g/kg to the the elderly medication group in 30 days. Meanwhile,drosophilas,as biological model,were given the lower(0.115 %),the medium(0.461 %),and the higher(1.845 %)concentration of pine needle powder added to the culture mediums and then observed the life related behavioral indexes of drosophilas. Compared with elderly model group,it showed that the pine needle powder could not only significantly increase the content of serum SOD(P<0.05)and GSH in liver tissue(P<0.05)but reduce the MDA in the elderly medication group. The results also showed that pine needle powder could prolong the lifespan,increase the offspring numbers,enhance the climbing ability and the anti-sub-acute oxidative damage capacity and body water content of the aging drosophilas. The wild pine needle powder in Changbai Mountain obtained antioxidant and anti-aging effects which could improve the liferelated indicators of aging mice and drosophilas.
引文
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[23]张培刚,郑鸿雁,昌友权,等.松针黄酮的体外抗氧化作用研究[J].食品科学,2005,26(9):488-490
[24]郑光耀,何玲,薄采颖,等.松针叶绿素-胡萝卜素软膏体外清除自由基及体内抗氧化作用的研究[J].时珍国医国药,2013,24(4):815-817
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[27]Cirelli C.Cellular consequences of sleep deprivation in the brain[J].Sleep Medicine Reviews,2006,10(5):307-321
[2]陈英,刘成国,赵毓芝,等.松针功能性成分及应用研究进展[J].安徽农业科学,2012,40(10):5994-5996
[3]俞林森.松针健身食品两则[J].今日科技,1989(3):16
[4]刘晓庚,陈梅梅.我国松针的开发利用研究及进展[J].林产化工通讯,2003,37(4):29-34
[5]王燕,赵毅.左归饮对老年小鼠抗氧化作用及胸腺脾脏指数的影响[J].中国实验方剂学杂志,2007,13(3):67-69
[6]刘天骥,张万明.中医药传统膏散剂配制方法初探[J].中医外治杂志,1997,22(6):36-37
[7]Lin W S,Chen J Y,Wang J C,et al.The anti-aging effects of Ludwigia octovalvis on Drosophila melanogaster and SAMP8 mice[J].Age,2014,36(2):689-703
[8]Zou Y X,Ruan M H,Luan J,et al.Anti-Aging Effect of Riboflavin Via Endogenous Antioxidant in Fruit fly Drosophila Melanogaster[J].Journal of Nutrition Health&Aging,2016,21(3):1-6
[9]刘莹,谢宁,徐丽,等.地黄饮子对果蝇攀爬能力的影响[J].中医药信息,2013,30(3):37-38
[10]Waqas M K,B A Khan,Akhtar N,et al.Fabrication of Tamarindus indica seeds extract loade-cream for photo-aged skin:Visioscan studies[J].Postepy Dermatologii Alergologii,2017,34(4):339-345
[11]边晓丽,王晓理,李金娜,等.6种抗衰老中药清除氧自由基和抗脂质过氧化作用的测定[J].西北药学杂志,2001,16(2):68-69
[12]Jiao S P,Chen B,Du P G.Anti-lipid peroxidation effect of Rosa davuriuca Pall.fruit[J].Journal of Chinese Integrative Medicine,2004,2(5):364-365
[13]Farag M R,Alagawany M,Abd E H,et al.Yucca schidigera extract modulates the lead-induced oxidative damage,nephropathy and altered inflammatory response and glucose homeostasis in Japanese quails[J].Ecotoxicology and environmental safety,2018,30(156):311-321
[14]赵蕾,杨关根,刘智勇,等.养血活血汤对老年性便秘小鼠血清及结肠组织匀浆中NO、MDA、SOD、GSH的影响[J].中华中医药学刊,2014,32(7):1708-1710
[15]Freeman B A,Crapo J D.Free Radicals and Tissue Injury[J].Laboratory Investigation,1982,47(5):412-426
[16]段丹丹,高丽,秦雪梅,等.果蝇在药理学研究中的应用[J].中国药学杂志,2016,51(19):1638-1642
[17]Mackay T F C.Mutations and quantitative genetic variation:lessons from Drosophila[J].Philosophical Transactions of the Royal Society of London,2010,365(1544):1229-1239
[18]刘力.果蝇:基因、脑和行为[J].世界科技研究与发展,2000,22(6):46-49
[19]Adams M D,Sekelsky J J.From sequence to phenotype:reverse genetics in Drosophila melanogaste[J].Nature Reviews Genetics,2002,3(3):189-198
[20]温艾,刘力.以果蝇模型研究人类神经退行性疾病[J].生物化学和生物物理进展,2003,30(3):357-362
[21]杨红彦,刘海燕,杨波,等.温度和相对湿度对果蝇寿命的影响[J].同济大学学报(医学版),2002,23(1):20-22
[22]王彩艳,周碧娇,田祯祥.培养基成分对雌果蝇寿命的影响[J].安徽农业科学,2014,42(6):1635-1638
[23]张培刚,郑鸿雁,昌友权,等.松针黄酮的体外抗氧化作用研究[J].食品科学,2005,26(9):488-490
[24]郑光耀,何玲,薄采颖,等.松针叶绿素-胡萝卜素软膏体外清除自由基及体内抗氧化作用的研究[J].时珍国医国药,2013,24(4):815-817
[25]陈菲,盛柳青,麻佳蕾.松针中原花青素的闪式提取及其抗氧化活性[J].中国医药工业杂志,2014,45(2):120-123
[26]Gilbert M J H,Zerulla T C,Tierney K B.Zebrafish(Danio rerio)as a modelforthe study of aging and exercise:physical ability and trainability decrease with age[J].Experimental Gerontology,2014,50(1):106-113
[27]Cirelli C.Cellular consequences of sleep deprivation in the brain[J].Sleep Medicine Reviews,2006,10(5):307-321