响应面法优化山荆子果实总黄酮的提取工艺
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摘要
目的:优化山荆子果实总黄酮提取方法。方法:对山荆子果实进行总黄酮提取,采用响应面法进行优化,以料液比、醇浓度、提取时间、提取次数作为考察因素,以总黄酮含量为考察指标,确定最优提取方法。结果:响应面法优化后提取工艺:料液比1∶20(g/ml)、醇浓度40%、提取时间30 min、提取次数1次总黄酮含量:17. 83%。结论:响应面法优化山荆子果实总黄酮的提取工艺行之有效,为后续刺玫果的开发利用提供了依据。
Objective: To optimize the total flavonoids extraction from Malus baccata( Linn.) Borkh. Methods: The most optimizing flavonids extraction from Malus baccata( Linn.) Borkh was decided by response surface methodology,using solid-liquid ratio,ethanol concentration,times and duration of sample extraction as parameters. Results: Based on ANOVA results,the following optimal extraction conditions were identified: the solid-liquid ratio was 1∶ 20( g/ml);the ethanol concentration was 40%; extraction duration was 30 min; and the total flavonoids yield reached 17. 83% after once extraction. Conclusion: Response surface methodology was effective in optimizing the extraction process of the total flavonoids from Malus baccata( Linn.) Borkh,which will provide the basis for the development and utilization of Rosa davurica Pall.
Objective: To optimize the total flavonoids extraction from Malus baccata( Linn.) Borkh. Methods: The most optimizing flavonids extraction from Malus baccata( Linn.) Borkh was decided by response surface methodology,using solid-liquid ratio,ethanol concentration,times and duration of sample extraction as parameters. Results: Based on ANOVA results,the following optimal extraction conditions were identified: the solid-liquid ratio was 1∶ 20( g/ml);the ethanol concentration was 40%; extraction duration was 30 min; and the total flavonoids yield reached 17. 83% after once extraction. Conclusion: Response surface methodology was effective in optimizing the extraction process of the total flavonoids from Malus baccata( Linn.) Borkh,which will provide the basis for the development and utilization of Rosa davurica Pall.
引文
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[18]郭洁,洪子雯,方晓玲. Box-Behnken实验设计法优化表阿霉素脂质体的处方工艺[J].复旦学报(医学版),2007(6):816-820.
[2]冯章丽,于文全,顾广军,等.东北部分地区山荆子种质资源表型多样性及聚类分析[J].植物遗传资源学报,2016,17(6):984-992.
[3]丁传波,赵婷,董岭,等.山荆子中根皮苷和黄酮含量的测定及其乙醇提取物的抗氧化活性[J].西北农林科技大学学报(自然科学版),2014,42(6):161-167.
[4]杨秀东,郭永真,周鸿立.山荆子化学成分与药理作用研究进展[J].吉林农业,2016(23):122.
[5]丁传波,刘璐璐,赵婷,等.山荆子叶总黄酮对糖尿病小鼠血糖、血脂的作用[J].食品科学,2014,35(17):226-230.
[6]丁传波,赵婷,李永娟,等.山荆子叶醇提物对四氯化碳致小鼠急性肝损伤的保护作用[J].中国药理学通报,2013,29(11):1627-1628.
[7]王璐,王振宇,李小雨,等.山荆子不同溶剂提取物的体外抗氧化及抗癌活性[J].食品科技,2013,38(9):201-204.
[8]姜凤.山荆子果与国光苹果中微量元素的测定[J].长春师范学院学报,2013,32(8):59-62.
[9]韩东秀,张志民.小兴安岭山荆子资源现状与经营对策[J].南北桥,2009(3):10.
[10]黄占波,宋冬梅,尚海巍,等.山荆子叶总黄酮提取工艺研究[J].安徽农业科学,2016,44(35):116-118.
[11]余小翠,刘高峰.响应面分析法在中药提取和制备工艺中的应用[J].中药材,2010,33(10):1651-1655.
[12]慕运动.响应面方法及其在食品工业中的应用[J].郑州工程学院报,2001(3):91-94.
[13]慕运动,朱碧,郭兴凤. RSM优化菜籽蛋白酶水解条件及结果分析[J].河南工业大学学报(自然科学版),2009,30(3):87-90.
[14]彭晓霞,路莎莎.响应面优化法在中药研究中的应用和发展[J].中国实验方剂学杂志,2011,17(19):296-299.
[15] Yoo S H,Song Y B,Chang P S,et al. Microencapsulation of alpha-tocopherol using sodium alginate and its controlled release properties[J].International Journal of Biological Macromolecules,2006,38(1):25.
[16] Yin G,Dang Y. Optimization of extraction technology of the Lycium barbarum polysaccharides by Box–Behnken statistical design[J].Carbohydrate Polymers,2008,74(3):603-610.
[17] Bezerra M A,Santelli R E,Oliveira E P,et al. Response surface methodology(RSM)as a tool for optimization in analytical chemistry[J]. Talanta,2008,76(5):965.
[18]郭洁,洪子雯,方晓玲. Box-Behnken实验设计法优化表阿霉素脂质体的处方工艺[J].复旦学报(医学版),2007(6):816-820.