钙离子和蓝光对深绿木霉Tr775在液体发酵过程中分生孢子产量的影响
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摘要
在深绿木霉(Trichoderma atroviride)Tr775液体发酵过程中添加钙离子(Ca~(2+))和蓝光(400~480 nm),考察Ca~(2+)添加量、Ca~(2+)添加时间、蓝光添加时间对其分生孢子产量的影响,并在10 L发酵罐中进行Ca~(2+)和蓝光的组合添加实验,考察Ca~(2+)和蓝光对分生孢子产量是否可产生协同效应。结果表明,当Ca~(2+)添加量从54.6 mmol·L~(-1)增加至127.4mmol·L~(-1)时,Tr775的产孢量显著(P≤0.05)高于Ca~(2+)添加量为0.0 mmol·L~(-1)和18.2 mmol·L~(-1)的处理;但当Ca~(2+)添加量达到163.8 mmol·L~(-1)时,Tr775产孢量出现显著(P≤0.05)下降。在发酵过程的不同时段分别添加Ca~(2+)(91.0mmol·L~(-1))或蓝光,Tr775产孢量随着发酵时间的延长而降低,在第72 h时添加Ca~(2+)或蓝光,Tr775产孢量与未添加Ca~(2+)或蓝光处理之间无显著性差异。与单独添加Ca~(2+)或蓝光相比较,在液体发酵的第0 h时同时添加Ca~(2+)和蓝光,对Tr775产孢量的增加无显著性影响。
We added calcium ions( Ca~(2+)) and blue light(400-480 nm) in the liquid fermentation process to investigate the effects of Ca~(2+)addition amount,Ca~(2+)adding time,and blue light adding time on conidium yield of Trichoderma atroviride strain Tr775. Moreover,we investigated the effect of adding Ca~(2+)and blue light together to determine if a synergistic reaction could occur on conidium yield during liquid fermentation in 10 L fermentors by the combined addition Ca~(2+)and blue light. The results showed that the conidium yield was significantly increased( P≤0. 05) in the treatments of Ca~(2+)addition amount from 54. 6 mmol·L~(-1) to 127. 4 mmol·L~(-1) compared to those of 0. 0 mmol·L~(-1) and 18. 2 mmol · L~(-1). However,the conidium yield was significantly decreased( P ≤ 0. 05) when Ca~(2+)addition amount reached 163. 8 mmol·L~(-1). After adding Ca~(2+)(91. 0 mmol·L~(-1)) or blue light at different times in the fermentation process,the conidium yield was gradually decreased as later as the adding time. When Ca~(2+)or blue light was added at 72 h,the conidium yield had no significant difference compared to the control treatment without adding Ca~(2+)or blue light.Compared with adding Ca~(2+)or blue light along,it had no significant difference in the conidium yield when Ca~(2+)and blue light were added together at 0 h in the fermentation process.
We added calcium ions( Ca~(2+)) and blue light(400-480 nm) in the liquid fermentation process to investigate the effects of Ca~(2+)addition amount,Ca~(2+)adding time,and blue light adding time on conidium yield of Trichoderma atroviride strain Tr775. Moreover,we investigated the effect of adding Ca~(2+)and blue light together to determine if a synergistic reaction could occur on conidium yield during liquid fermentation in 10 L fermentors by the combined addition Ca~(2+)and blue light. The results showed that the conidium yield was significantly increased( P≤0. 05) in the treatments of Ca~(2+)addition amount from 54. 6 mmol·L~(-1) to 127. 4 mmol·L~(-1) compared to those of 0. 0 mmol·L~(-1) and 18. 2 mmol · L~(-1). However,the conidium yield was significantly decreased( P ≤ 0. 05) when Ca~(2+)addition amount reached 163. 8 mmol·L~(-1). After adding Ca~(2+)(91. 0 mmol·L~(-1)) or blue light at different times in the fermentation process,the conidium yield was gradually decreased as later as the adding time. When Ca~(2+)or blue light was added at 72 h,the conidium yield had no significant difference compared to the control treatment without adding Ca~(2+)or blue light.Compared with adding Ca~(2+)or blue light along,it had no significant difference in the conidium yield when Ca~(2+)and blue light were added together at 0 h in the fermentation process.
引文
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[18]梁巧兰,韩亮,周其宇.深绿木霉T2发酵液蛋白分离物诱导抗病作用研究[J].草原与草坪,2016,36(3):28-34.
[19]苏捷,朱俊晨,杨汶德.蓝光对真菌的调控作用及研究进展[J].微生物学杂志,2006,26(2):82-85.
[20]FOSTER J W,Mc DANIEL L E,WOODRUFF H B,et al.Microbiological aspects of penicillin.Ⅴ.conidiospore formation in submerged cultures of Penicillium notatum[J].Journal of Bacteriology,1945,50(3):365-368.
[21]相启森,岳田利,袁亚宏,等.金属离子对褐黄孢链霉菌生长和纳他霉素生物合成的影响[J].西北农林科技大学学报(自然科学版),2010,38(1):215-221.
[2]曾华兰,叶鹏盛,李琼芳.中药材土传病害拮抗的筛选与应用初探[J].云南农业大学学报,2002,17(4):386-388.
[3]李兆防.深绿木霉T95和球毛壳ND35混用对土传病害的防治效果[J].中国蔬菜,2010(18):64-68.
[4]郭润芳,史盛高,高宝嘉,等.木霉菌在植物生物防治中的应用[J].河北林果研究,2001,16(3):294-298.
[5]高克祥,刘晓光,郭润芳,等.木霉菌对五种植物病原真菌的重寄生作用[J].山东农业大学学报(自然科学版),2002,33(1):37-42.
[6]池玉杰,伊洪伟,艾志强,等.深绿木霉产孢条件优化[J].中国农学通报,2013,29(28):42-45.
[7]潘玮,穆长清,蒋细良,等.木霉菌厚垣孢子与分生孢子研究进展及商品化制剂[C]//农业生物灾害预防与控制研究,2005:652-655.
[8]LAUTER F R,RUSSO V E A.Blue light induction of conidiation-specific genes in Neurospora crassa[J].Nucleic Acids Research,1991,19(24):6883-6886.
[9]LING J,WELLS D R,TANGUAY R L,et al.Heat shock protein HSP101 binds to the Fed-l internal light regulatory element and mediates its high translational activity[J].The Plant Cell,2000,12:1213-1227.
[10]BERGMAN K.Blue-light control of sporangiophore initiation in Phycomyces[J].Planta,1972,107(1):53-67.
[11]付鸣佳,邹峥嵘.蓝光诱导拟盘多毛孢菌分生孢子器产生和类胡萝卜素的积累[J].食品科学,2009,30(7):118-121.
[12]朱俊晨,王小菁.蓝光促进黑曲霉分生孢子发育和产糖化酶的研究[J].微生物学报,2005,45(2):275-278.
[13]KERTESZ-CHALOUPKOVA K,WALSER P J,GRANADO J D,et al.Blue light overrides repression of asexual sporulation by mating type genes in the basidiomycete Coprinus cinereus[J].Fungal Genetics and Biology,1998,23(1):95-109.
[14]GRIMM L H,KELLY S,KRULL R,et al.Morphology and productivity of filamentous fungi[J].Appl Microbiol Biotechnol,2005,69(4):375-384.
[15]KOSSEN N W F.The morphology of filamentous fungi[J].Advances in Biochemical Engineering/Biotechnology,2000,24(70):1-33.
[16]ZNIDARSiC P,PAVKO A.The morphology of filamentous fungi in submerged cultivations as a bioprocess parameter[J].Food Technology&Biotechnology,2001,39(3):237-252.
[17]ROSON G D,WIEBE M G,TRINCI A P J.Involvement of Ca2+in the regulation of hyphal extension branching in Fusarium graminearum A 3/5[J].Experimental Mycology,1991,15(3):263-272.
[18]梁巧兰,韩亮,周其宇.深绿木霉T2发酵液蛋白分离物诱导抗病作用研究[J].草原与草坪,2016,36(3):28-34.
[19]苏捷,朱俊晨,杨汶德.蓝光对真菌的调控作用及研究进展[J].微生物学杂志,2006,26(2):82-85.
[20]FOSTER J W,Mc DANIEL L E,WOODRUFF H B,et al.Microbiological aspects of penicillin.Ⅴ.conidiospore formation in submerged cultures of Penicillium notatum[J].Journal of Bacteriology,1945,50(3):365-368.
[21]相启森,岳田利,袁亚宏,等.金属离子对褐黄孢链霉菌生长和纳他霉素生物合成的影响[J].西北农林科技大学学报(自然科学版),2010,38(1):215-221.