草莓抗枯萎病分子标记初定位
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摘要
为加快草莓(Fragaria×ananassa Duch.)枯萎病抗病分子育种进程,以草莓优系后代为试材进行抗病试验分析,对父母本及不同优系后代进行抗病基因的筛选,并结合SSR分子标记技术,最终筛选出抗病品系。结果表明,抗病试验中,优系13-53-100和13-52-407具有很强的抗枯萎病特性,优系13-65-265抗枯萎病特性最差;抗病基因筛选出亲本中紫金四季和甜查理含有抗病性基因,优系后代中13-53-100和13-52-407含有抗病基因,可能遗传了甜查理的抗病性。
In order to speed up the molecular breeding process of strawberry resistance(Fragaria×ananassa Duch.), the excellent hybrid offspring of strawberry were used as the test materials. At first,the resistance test was carried out, and the resistant genes of parents and different hybrid offsprings were screened, combined with SSR molecular marker technology. Finally,the resistant strains were screened out. The results showed that excellent offspring 13-53-100 and 13-52-407 had strong resistance to F. oxysporum, and excellent offspring 13-65-265 had the worst resistance to F. oxysporum. The screening result of resistance gene showed that, the parent Zijin Sijin and sweet Charlie had the resistance gene, the offspring 13-53-100 and 13-52-407 had a consistent band of parent sweet Charlie, which may have inherited the resistance of sweet Charlie.
In order to speed up the molecular breeding process of strawberry resistance(Fragaria×ananassa Duch.), the excellent hybrid offspring of strawberry were used as the test materials. At first,the resistance test was carried out, and the resistant genes of parents and different hybrid offsprings were screened, combined with SSR molecular marker technology. Finally,the resistant strains were screened out. The results showed that excellent offspring 13-53-100 and 13-52-407 had strong resistance to F. oxysporum, and excellent offspring 13-65-265 had the worst resistance to F. oxysporum. The screening result of resistance gene showed that, the parent Zijin Sijin and sweet Charlie had the resistance gene, the offspring 13-53-100 and 13-52-407 had a consistent band of parent sweet Charlie, which may have inherited the resistance of sweet Charlie.
引文
[1]宁志怨,董玲,廖华俊,等.草莓枯萎病的遗传与育种研究进展[J].分子植物育种,2015,13(1):235-240.
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[6]曹娴,高清华,聂京涛,等.草莓灰霉病抗性遗传分析和分子标记初定位[J].上海交通大学学报,2011,29(4):75-78,86.
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[10]刘建成,段可,张庆雨,等.草莓抗白粉病遗传图谱及其SSR标记初分析[J].江西农业学报,2012,24(11):49-52.
[11]李静.草莓抗炭疽病遗传图谱构建和抗性相关基因的克隆与表达分析[D].江苏南京:南京农业大学,2012.
[2]吉沐祥,李国平,杨敬辉,等.江苏省大棚草莓生产中存在的问题与技术创新[J].江西农业学报,2012,24(2):58-60,66.
[3]吉沐祥,杨敬辉,吴祥,等.大棚草莓主要病害发生规律与绿色防控措施[J].江苏农业科学,2012,40(10):114-117.
[4]王传芹,袁英国.长丰地区草莓枯萎病的发生及综合防治[J].现代农业科技,2008(19):165.
[5]宁志怨,董玲,廖华俊,等.草莓枯萎病的研究进展[J].草莓研究进展,2015(4):386-393.
[6]曹娴,高清华,聂京涛,等.草莓灰霉病抗性遗传分析和分子标记初定位[J].上海交通大学学报,2011,29(4):75-78,86.
[7]刘建成.草莓NBS-LRR家族基因的克隆和表达分析与白粉病抗性相关分子标记的研究[D].宁夏银川:宁夏大学,2011.
[8]曹娴.草莓抗灰霉病基因定位及榉树种质资源多样性的ISSR分析[D].上海:上海交通大学,2011.
[9]赵密珍.草莓种质资源描述规范和数据标准[M].北京:中国农业出版社,2006.
[10]刘建成,段可,张庆雨,等.草莓抗白粉病遗传图谱及其SSR标记初分析[J].江西农业学报,2012,24(11):49-52.
[11]李静.草莓抗炭疽病遗传图谱构建和抗性相关基因的克隆与表达分析[D].江苏南京:南京农业大学,2012.