宁心宝胶囊指纹图谱研究及4种核苷类成分的含量测定
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摘要
目的:研究宁心宝胶囊的HPLC指纹图谱,并建立同时测定宁心宝胶囊中尿嘧啶、尿苷、腺嘌呤、腺苷含量的方法。方法:采用Agilent Zorbax SB-Aq C_(18)色谱柱(250 mm×4.6 mm,5μm),甲醇-0.05 mol·L~(-1)磷酸二氢钾水溶液为流动相,梯度洗脱,流速为1.0 ml·min~(-1),检测波长为212 nm(指纹图谱)、260 nm(含量测定),柱温为30℃。对10批宁心宝胶囊进行了指纹图谱及含量测定研究,采用中药色谱指纹图谱相似度评价软件进行分析。结果:建立了宁心宝胶囊的HPLC指纹图谱,标定了10个共有色谱峰。4种核苷类成分的分离度良好。结论:此方法简便、可靠,指纹图谱结合含量测定可以更好地控制宁心宝胶囊的内在质量。
Objective: To study the HPLC fingerprint of Ningxinbao capsules,and establish a method for the simultaneous content determination of uracil,uridine,adenine and adenosine. Methods: The separation was carried out on an Agilent Zorbax SB-Aq C_(18) column(250 mm × 4. 6 mm,5 μm) with gradient elution using methanol-0. 05 mol·L~(-1)potassium dihydrogen phosphate at 30℃ and at a flow rate of 1. 0 ml·min~(-1),the detection wavelength was 212 nm for fingerprint and 260 nm for the determination of the four nucleosines. Totally 10 batches of samples were analyzed with the developed HPLC fingerprint and the determination method,the data calculation was performed with similarity evaluation system in the chromatographic fingerprint of TCM. Results: In the fingerprint,10 common peaks were marked and the separation of the four nucleosines was good. Conclusion: The method is simple and reliable. The HPLC fingerprint and contents of the four nucleosines in Ningxinbao capsules can be used for the quality control.
Objective: To study the HPLC fingerprint of Ningxinbao capsules,and establish a method for the simultaneous content determination of uracil,uridine,adenine and adenosine. Methods: The separation was carried out on an Agilent Zorbax SB-Aq C_(18) column(250 mm × 4. 6 mm,5 μm) with gradient elution using methanol-0. 05 mol·L~(-1)potassium dihydrogen phosphate at 30℃ and at a flow rate of 1. 0 ml·min~(-1),the detection wavelength was 212 nm for fingerprint and 260 nm for the determination of the four nucleosines. Totally 10 batches of samples were analyzed with the developed HPLC fingerprint and the determination method,the data calculation was performed with similarity evaluation system in the chromatographic fingerprint of TCM. Results: In the fingerprint,10 common peaks were marked and the separation of the four nucleosines was good. Conclusion: The method is simple and reliable. The HPLC fingerprint and contents of the four nucleosines in Ningxinbao capsules can be used for the quality control.
引文
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2 雷宁,杜树山,倪雪梅,等.RP-HPLC测定天然虫草与人工虫草中核苷类成分的含量[J].中国药学杂志,2006,41(12):948-951
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8 李建银,刘文,邱德文.中药复方指纹图谱的研究现状及其前景[J].武警医学,2004,15(1):59-61
9 张欢,刘祯.中药有效成分研究[J].黑龙江科技信息,2009,11(10):260-261
10 胡爱萍.关于中药药性理论现代化研究的思考[J].光明中医,2006,21(10):20-22
2 雷宁,杜树山,倪雪梅,等.RP-HPLC测定天然虫草与人工虫草中核苷类成分的含量[J].中国药学杂志,2006,41(12):948-951
3 Jin WB,Leonard L,Jeff CH.Antitumor sterols from the mycelia of Cordyceps sinensis[J].Phytochemistry,1999,51:891-898
4 张智,谢意珍,李森柱,等.冬虫夏草研究进展.微生物学杂志[J].2002,22(5):50-55
5 王海南.中药有效成分研究与中药新药研发[J].中华中医药杂志,2007,22(5):268-270
6 韩晔华,霍飞凤,杨悠悠,等.中药指纹图谱研究的某些进展与展望[J].色谱,2008,26(2):142-152
7 张霞,蓝苑元,任萃文.中药复方制剂制备工艺研究进展[J].中国现代中药,2007,9(4):38-40
8 李建银,刘文,邱德文.中药复方指纹图谱的研究现状及其前景[J].武警医学,2004,15(1):59-61
9 张欢,刘祯.中药有效成分研究[J].黑龙江科技信息,2009,11(10):260-261
10 胡爱萍.关于中药药性理论现代化研究的思考[J].光明中医,2006,21(10):20-22