超高效液相色谱法同时测定黄芩提取物多指标成分的含量
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摘要
目的建立黄芩提取物中4种有效成分(黄芩苷、汉黄芩苷、黄芩素和汉黄芩素)同时定量的方法。方法采用超高效液相色谱以ACQUITY UPLCTMBEH C18色谱柱(1.7μm,100×2.1mm),乙腈-0.1%甲酸溶液进行梯度洗脱,测定波长280 nm,柱温30℃,流速0.2 ml/min。结果 4种成分的线性关系良好,精密度、稳定性、重复性的RSD均低于3.0%,加样回收率为96.2%~99.6%。结论该方法具有简便、灵敏、准确的特点,适用于产品质量的控制。
Objective To establish Scutellaria extracts of four active ingredients( baicalin,wogonoside,baicalein,wogonin) by simultaneous quantitative methods. Methods The chromatographic separation was achieved on a ACQUITY UPLCTMBEH C18column( 1. 7μm,2. 1 × 100mm),the mobile phase was Acetonitrile-0. 1% formic acid aqueous solution with gradient elution at a flow rate of 0. 2 ml / min,the detection wavelength was set at 280 nm,and the column temperature was 30℃. Results It showed a good linear relationship of four components,precision,stability,repeatability RSD were less than 3. 0%,recovery was 96. 2% ~99. 6%. Conclusion The method is simple,sensitive,accurate and can be used for quality control Scutellaria extracts.
Objective To establish Scutellaria extracts of four active ingredients( baicalin,wogonoside,baicalein,wogonin) by simultaneous quantitative methods. Methods The chromatographic separation was achieved on a ACQUITY UPLCTMBEH C18column( 1. 7μm,2. 1 × 100mm),the mobile phase was Acetonitrile-0. 1% formic acid aqueous solution with gradient elution at a flow rate of 0. 2 ml / min,the detection wavelength was set at 280 nm,and the column temperature was 30℃. Results It showed a good linear relationship of four components,precision,stability,repeatability RSD were less than 3. 0%,recovery was 96. 2% ~99. 6%. Conclusion The method is simple,sensitive,accurate and can be used for quality control Scutellaria extracts.
引文
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[3]李玉山.黄芩的化学成分及黄芩苷的提取方法[J].西北药学杂志,2008,23(6):76.
[4]赵胜男,李守拙.不同采收期黄芩中黄酮类成分含量比例的研究[J].中国实验方剂杂志,2012,18(21):96.
[5]宋双红,王炳利,冯军康,等.不同加工方法对黄芩炮制品质量影响的研究[J].中药材,2006,29(9):20.
[6]李冰岚,陈宗良,蒋士鹏.HPLC法测定复方蒲芩片中黄芩苷的含量[J].药物分析杂志,2010,30(4):703.
[7]杨振伟,张英,屈战果.HPLC法测定宁神灵颗粒中黄芩苷的含量[J].药物分析杂志,2006,26(11):79.
[8]谢东,路玫.反相高效液相色谱法同时测定双黄消炎片中黄芩苷和盐酸小檗碱的含量[J].药物分析杂志,2008,28(5):140.
[9]宋双红,张媛.黄芩药材HPLC指纹图谱的研究[J].中国药学杂志,2006,41(6):18.
[10]冯波,施春玲,郝乘仪等.RP-HPLC法测定芎菊上清丸中黄芩苷的含量[J].中草药,2009,40(5):743.
[11]王西彬,陈畅,何希荣.RP-HPLC测定三黄片中黄芩苷和盐酸小檗碱的含量[J].中国实验方剂学杂志,2012,18(13):128.
[12]李文仕,甘妮.RP-HPLC测定乙肝解毒胶囊中黄芩苷的含量[J].药物分析杂志,2008,28(2):150.
[13]刘征辉,叶挺祥,赵洪芝.黄连羊肝片质量标准研究[J].中成药,2009,31(8):附3.
[14]范全民,孙冬云,周慧娟.HPLC测定黄连上清片中黄芩苷的含量[J].中成药,2006,28(10):1545.
[15]徐国兵,关荣才.RP-HPLC测定化瘀祛斑胶囊中黄芩苷的含量[J].中国药学杂志,2002,37(2):133.
[16]吴建龙,简炎林.反相高效液相色谱法测定黄连解毒片中黄芩苷的含量[J].广东药学院学报,2004,20(3):242.