杜鹃花属植物的可育性研究进展
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摘要
结合作者近年来的工作,总结了国内外有关杜鹃花属(Rhododendron)植物可育性研究的成果和进展,旨在为相关研究者和技术人员提供参考。(1)杜鹃花属的杜鹃亚属(subgen.Rhododendron)和羊踯躅亚属(subgen.Pentanthera)中存在多倍化现象,甚至存在同一物种有多种倍性。(2)在自然授粉条件下,除了常见种内杂交及自交情形外,常绿杜鹃亚属(subgen. Hymenanthes)、杜鹃亚属和映山红亚属(subgen. Tsutsusi)内种间杂交十分普遍,也有跨亚组之间的自然杂交案例。(3)杜鹃花属植物中自交亲和(self compatibility, SC)与自交不亲和(self incompatibility,SI)现象并存,云锦杜鹃亚组(subsect.Fortunea)兼具自交败育到高可育性的所有类型,而在映山红亚属与马银花亚属(subgen. Azaleastrum)中自交不亲和现象则更为常见。通常自交被认为是杜鹃花物种的被动适应,但对于一些在极端气候条件下分布的局域种而言,则可能是一种主动响应。(4)不同分类等级(亚属、组和亚组)间的人工杂交研究表明,杂交亲和性或可育性与双亲的系统发育关系及染色体倍性具有明显的关联,并反映在发育阶段和各项亲和力指标的变化之中。常绿杜鹃亚属与杜鹃亚属间的远缘杂交相对比较容易,常绿杜鹃亚属与映山红亚属间、映山红亚属与杜鹃亚属的正反交、映山红亚属与羊踯躅亚属的正交以及常绿杜鹃亚属与羊踯躅亚属的正交、马银花亚属与杜鹃亚属正交等也有亲和的案例。(5)自交不亲和亲本的介入可大幅度增加种间杂交的不育比率,并导致其由双向可育到单向不育再到双向不育方向发展。
To provide a reference for researchers, achievements and progress in recent years on the fertility of Rhododendron in China and abroad were summarized.(1) There are polyploidy in subgen. Rhododendron and subgen. Pentanthera, and even various ploidy in the same species.(2) Under natural pollination, in addition to the common intra-specific cross-and self-fertilization, inter-specific cross-fertilization was also widespread in subgen. Hymenanthes, subgen. Rhododendron and subgen. Tsutsusi. There were also some cases of inter-subsection cross.(3) Self compatibility(SC) and self incompatibility(SI) simultaneous exist in Rhododendron. Subsect. Fortunea shows all types from self sterile to high fertility. Self incompatibility often occurs in subgen. Tsutsusi and subgen. Azaleastrum and although self-fertilization is usually considered to be a passive adaptation, it could be an active response to the adverse environmental conditions for a few local species.(4) Through artificial crosses between different taxa(subgenera, sections and subsections), the inter-specific crossability was obviously relevant to the relationship of phylogenetic development and chromosomal ploidy of parents. This was reflected in the changes of indices of compatibility in different phases of development and was discovered that hybridization was easier between distant subgen.Hymenanthes and subgen. Rhododendron. There were also some cases of compatibility such as the reciprocal crosses between subgen. Hymenanthes and subgen. Tsutsusi, subgen. Tsutsusi and subgen. Rhododendron as well as the unidirectional compatibility between subgen. Tsutsusi and subgen. Pentanthera, subgen.Hymenanthes and subgen. Pentanthera, subgen. Azaleastrum and subgen. Rhododendron.(5) It leads to increases in the fertility and development from bidirectional fertility to unidirectional sterility and then bidirectional sterility in inter-specific cross as SI parents get involved.
To provide a reference for researchers, achievements and progress in recent years on the fertility of Rhododendron in China and abroad were summarized.(1) There are polyploidy in subgen. Rhododendron and subgen. Pentanthera, and even various ploidy in the same species.(2) Under natural pollination, in addition to the common intra-specific cross-and self-fertilization, inter-specific cross-fertilization was also widespread in subgen. Hymenanthes, subgen. Rhododendron and subgen. Tsutsusi. There were also some cases of inter-subsection cross.(3) Self compatibility(SC) and self incompatibility(SI) simultaneous exist in Rhododendron. Subsect. Fortunea shows all types from self sterile to high fertility. Self incompatibility often occurs in subgen. Tsutsusi and subgen. Azaleastrum and although self-fertilization is usually considered to be a passive adaptation, it could be an active response to the adverse environmental conditions for a few local species.(4) Through artificial crosses between different taxa(subgenera, sections and subsections), the inter-specific crossability was obviously relevant to the relationship of phylogenetic development and chromosomal ploidy of parents. This was reflected in the changes of indices of compatibility in different phases of development and was discovered that hybridization was easier between distant subgen.Hymenanthes and subgen. Rhododendron. There were also some cases of compatibility such as the reciprocal crosses between subgen. Hymenanthes and subgen. Tsutsusi, subgen. Tsutsusi and subgen. Rhododendron as well as the unidirectional compatibility between subgen. Tsutsusi and subgen. Pentanthera, subgen.Hymenanthes and subgen. Pentanthera, subgen. Azaleastrum and subgen. Rhododendron.(5) It leads to increases in the fertility and development from bidirectional fertility to unidirectional sterility and then bidirectional sterility in inter-specific cross as SI parents get involved.
引文
Akihide O,Kenichi S(2004)Cross incompatibility between Rhododendron seat.Tsutsusi species and Rhododendron japonicum(A.Gray)T.V.Suringar f.flavum Nakai.Journal of the Japanese Society for Horticultural Science,73,453-459.
Akihide O,Hiroshi I,Kenichi S(2006)Variation in crossability among Rhododendron japonicum f.flavum plants as pollen parents when crossed with evergreen species,R.eriocarpum.Journal of the Japanese Society for Horticultural Science,75,270-272.
Akihide O,Kenichi S(2006)Crossability of Kurume Azaleas as a seed parent for hybridizing with Rhododendron japonicum(A.Gray)J.V.Suringar f.flavum Nakai.Journal of the Japanese Society for Horticultural Science,75,191-198.
Akiko O,Ikumi D,Takashi S(2008)Bumblebee pollination and reproductive biology of Rhododendron semibarbatum(Ericaceae).Journal of Plant Research,121,319-327.
Akira SH(2010)Kinship between parents reduces offspring fitness in a natural population of Rhododendron brachycarpum.Annals of Botany,105,637-646.
Ammal EK(1950)Polyploidy in the genus Rhododendron.The Rhododendron Year Book,5,92-96.
Ammal EK,Enoch IC,Bridgwater M(1950)Chromosome numbers in species of Rhododendron.The Rhododendron Year Book,5,78-91.
Brown GK,Craven LA,Udovicic F,Ladiges PY(2006)Phylogeny of Rhododendron section Vireya(Ericaceae)base on two non-coding regions of cp DNA.Plant Systematics and Evolution,257,57-93.
Chamberlain DF,Hyam R,Argent G,Fairweather G,Walter KS(1996)The Genus Rhododendron,Its Classification and Synonymy.Royal Botanic Garden Edinburgh,Edinburgh.
Escaravage N,Wagner J(2004)Pollination effectiveness and pollen dispersal in a Rhododendron ferrugineum(Ericaceae)population.Plant Biology,6,606-615.
Fang MY,Fang RZ,He MY,Hu LZ,Yang HB(2005)Rhododendron.In:Flora of China(eds Wu ZY,Raven PH,Hong DY),Vol.14,pp.83-87.Science Press,Beijing&Missouri Botanical Garden Press,St.Louis.
Fang RZ,Min TL(1981)The influence of uplift of Himalayas on the floristic formation of genus Rhododendron.Acta Botanica Yunnanica,3,147-157.(in Chinese with English abstract)[方瑞征,闵天禄(1981)喜玛拉雅山脉的隆升对杜鹃属区系形成的影响.云南植物研究,3,147-157.]
Fang RZ,Min TL(1995)The floristic study on the genus Rhododendron.Acta Botanica Yunnanica,17,359-379.(in Chinese with English abstract)[方瑞征,闵天禄(1995)杜鹃属植物区系的研究.云南植物研究,17,359-379.]
Gaku K(1993)Relationships between flowering time and fruit set of the entomophilous alpine shrub,Rhododendron aureum(Ericaceae),inhabiting snow patches.American Journal of Botany,80,1300-1304.
Gaku K,Akira SH,Yuka K(2011)Pollination efficiency of bumblebee queens and workers in the alpine shrub Rhododendron aureum.International Journal of Plant Sciences,172,70-77.
Gao LM,Zhang CQ,Li DZ,Wu D(2005)Chromosome numbers of Rhododendron subgen.Azaleastrum.Acta Botanica Yunnanica,27,433-436.(in Chinese with English abstract)[高连明,张长芹,李德铢,吴丁(2005)杜鹃花属马银花亚属一些种类染色体数目报道.云南植物研究,27,433-436.]
Geng XM,Zhao HJ,Wu YQ,Zhang YM(2017)Cross-compatibility of wild Rhododendron and the effective evaluation indicators.Guihaia,37,979-988.(in Chinese with English abstract)[耿兴敏,赵红娟,吴影倩,张月苗(2017)野生杜鹃杂交亲和性及适宜的评价指标.广西植物,37,979-988.]
Gillian KB,Lyn AC,Frank U,Pauline YL(2006)Phylogenetic relationship of Rhododendron section Vireya(Ericaceae)interred from the ITS nrDNA region.Australian Systematic Botany,19,329-342.
Goetsch L,Eckert AJ,Hall BD(2005)The molecular systematics of Rhododendron(Ericaceae):A phylogeny based upon RPB2 gene sequences.Systematic Botany,30,616-626.
Griffin CAM,Eckert CG(2003)Experimental analysis of biparental inbreeding in a self-fertilizing plant.Evolution,57,1513-1519.
Hirao AS,Kameyama Y,Ohara M,Isagi Y,Kudo G(2006)Seasonal changes in pollinator activities influence pollen dispersal and seed production of the alpine shrub Rhododendron aureum(Ericaceae).Molecular Ecology,15,1165-1173.
Huang CC,Hung KH,Hwang CC(2011)Genetic population structure of the alpine species Rhododendron pseudochrysanthum,sensu lato(Ericaceae)inferred from chloroplast and nuclear DNA.BMC Evolutionary Biology,11,108-124.
Huang ZH,Song YP,Huang SQ(2017)Evidence for passerine bird pollination in Rhododendron species.Ao B Plant,9,1-10.
Jose AM,Juan A,Fernando O(2002)Reproductive ecology of Rhododendron ponticum(Ericaceae)in relict Mediterranean populations.Botanical Journal of the Linnean Society,140,297-311.
Kaori S,Ozaki Y,Ureshino K,Miyajima I,Wakana A,Okubo H(2008)Interploid crossing overcomes plastome-nuclear genome incompatibility in intersubgeneric hybridization between evergreen and deciduous azaleas.Scientia Horticulturae,115,268-274.
Karlsson PS,Jacobson A(2001)Onset of reproduction in Rhododendron lapponicum shoots:The effect of shoot size,age,and nutrient status at two subarctic sites.Oikos,94,279-286.
Kehr AE(1977)Azaleodendron breeding.Journal of American Rhododendron Society,31,226-232.
Kenji U,Miyoko K,Ikuo M(2000)Factors of intersectional unilateral cross incompatibility between several evergreen azalea species and Rhododendron japonicum f.flavum.Journal of the Japanese Society for Horticultural Science,69,261-265.
Kenji U,Yoshiko T,Yuka T,Takeru Ii,Ikue M,Masaru A(2006)Cross compatibility of intersubgeneric hybrids of azaleas on backcross with several evergreen species.Journal of the Japanese Society for Horticultural Science,75,403-409.
Koichi K,Yuji K,Tomohisa Y,Shigeo N,Takashi H(2005)Plastid inheritance and plastome-genome incompatibility of intergeneric hybrids between Menziesia and Rhododendron.Journal of the Japanese Society for Horticultural Science,74,318-323.
Kurashige Y,Etoh JI,Handa T,Takayamagi K,Yukawa T(2001)Section relationships in genus Rhododendron(Ericaceae):Evidence from mat K and trn K intron sequences.Plant Systematic Evolution,228,1-14.
Lan X,Zhang LH,Zhang JZ,Cui HX,Jiang CD,Shi L(2012)Research progress of Rhododendron breeding.Acta Horticulturae Sinica,39,1829-1838.(in Chinese with English abstract)[兰熙,张乐华,张金政,崔红霞,姜闯道,石雷(2012)杜鹃花育种研究进展.园艺学报,39,1829-1838.]
Levins R(1970)Extinction.In:Some Mathematical problems in Biology(ed.Gerstenhaber M),pp.77-107.American Mathematical Society Rhode Island,Providence.
Li SH,Sun WB,Ma YP(2018)Current conservation status and reproductive biology of the giant tree Rhododendron in China.Nordic Journal of Botany,doi:10.1111/njb.01999.
Li XS,Ba CY,Cao HN,Zong CG,Qu L,Zhang JZ(2015)Interspecific cross compatibility of Rhododendron in Changbai Mountain.Agricultural Science&Techno,16,1617-1620.
Liu DF,Xue YB(1997)Plant self-incompatibility.In:Genetics of Plant Reproduction(ed.Meng JL),pp.214-277.Science Press,Beijing.(in Chinese)[刘定富,薛永彪(1997)植物自交不亲和性,214-277页.见:植物生殖遗传学(孟金陵主编).科学出版社,北京.]
Liu XQ,Su JL,Li C,Liu XH(2010)Study on the fruitfulness of hybridization,selfing and open pollination of Rhododendron.Acta Agriculture Shanghai,26,145-148.(in Chinese with English abstract)[刘晓青,苏家乐,李畅,刘晓宏(2010)杜鹃花自交、杂交及开放授粉结实性研究.上海农业学报,26,145-148.]
Liu YF,Xing M,Zhao W,Fan RY,Luo S,Chen X(2012)Genetic diversity analysis of Rhododendron aureum Georgi(Ericaceae)located on Changbai Mountain using ISSR and RAPD markers.Plant Systematic Evolution,298,921-930.
Ma YP,Zhang CQ,Zhang JL,Yang YB(2010)Natural hybridization between Rhododendron delavayi and R.cyanocarpum(Ericaceae),from morphological,molecular and reproductive evidence.Journal of Integrative Plant Biology,52,844-851.
Ma YP,Xie WJ,Sun WB,Marczewski T(2016)Strong reproductive isolation despite occasional hybridization between a widely distributed and a narrow endemic Rhododendron species.Scientific Reports,6,19146.
Meng JL(1997)Genetics of Plant Reproduction,pp.296-357.Science Press,Beijing.(in Chinese)[孟金陵(1997)植物生殖遗传学,296-357页.科学出版社,北京.]
Milne RI,Terzioglu S,Abbott RJ(2003)A hybrid zone dominated by fertile F1s:Maintenance of species barriers in Rhododendron.Molecular Ecology,12,2719-2729.
Milne RI,Davies C,Prickett R,Inns LH(2010)Phylogeny of Rhododendron subgenus Hymenanthes based on chloroplast DNA markers:Between-lineage hybridisation during adaptive radiation?Plant Systematic Evolution,285,233-244.
Min TL,Fang RZ(1979)On the origin and geographic distribution of genus Rhododendron L.Acta Botanica Yunnanica,1,121-127.(in Chinese with English abstract)[闵天禄,方瑞征(1979)杜鹃属Rhododendron L.的地理分布及起源问题的探讨.云南植物研究,1,121-127.]
Min TL,Fang RZ(1990)The phylogeny and evolution of genus Rhododendron.Acta Botanica Yunnanica,12,353-365.(in Chinese with English abstract)[闵天禄,方瑞征(1990)杜鹃属的系统发育与进化.云南植物研究,12,353-365.]
Nathannel TW,Erin ED,Joseph BF,Stefan HG,David AM,Justin GS,Timothy MS,Sarah LR,Andrew GZ(2006)Pollinator limitation,autogamy and minimal inbreeding depression in insect-pollinated plants on a boreal island.American Midland Naturalist,155,19-38.
Ng SC,Corlett RT(2000)Comparative reproductive biology of the six species of Rhododendron(Ericaceae)in Hong Kong,South China.Canadian Journal of Botany,78,221-229.
Nobuo K,Daiki M,Akira N,Masaru A(2008)Attaining inter-subgeneric hybrids in fragrant azalea breeding and the inheritance of organelle DNA.Euphytica,159,67-72.
Oetsch LG,Eckert AJ,Hall BD(2005)The molecular systematics of Rhododendron(Ericaceae):A phylogeny based upon RPB2 gene sequences.Systematic Botany,30,616-626.
Palser BF,Rouse JL and Williams EG(1989)Coordinated timetables for megagametophyte development and pollen tube growth in Rhododendron nuttallii from anthesis to early postfertilization.American Journal of Botany,76,1167-1202.
Pierce L(1974)An unusual intergeneric cross.Journal of American Rhododendron Society,28,45-50.
Pornon A,Escaravage N,Boche TB(1997)Variation of reproductive traits in Rhododendron ferrugineum L.(Ericaceae)population along a successional gradient.Plant Ecology,130,1-11.
Richard JA,Juliet KJ,Richard IM,Amanda CM(2003)Plant introduction,hybridization and gene flow.Philosophical Transactions of the Royal Society B:Biological Sciences,358,1123-1132.
Rouse JL,Knox RB,Williams EG(1993)Inter-and intraspecific pollinations involving Rhododendron species.Journal of American Rhododendron Society,47,23-28.
Ruth A,Kwiton J,George A(2000)Chromosome numbers of some tropical Rhdodendrons(Section Vireya).Edinburgh Journal of Botany,57,1-7.
Sax K(1930)Chromosome stability in the genus Rhododendron.American Journal of Botany,17,247-251.
Schepper SD,Leus L,Mertens M,Bockstaele EV,Loose MD,Debergh P,Heursel J(2001)Flow cytometric analysis of ploidy in Rhododendron(subgenus Tsutsusi).Hortscience,36,125-127.
Shrestha,N,Wang ZH,Su XY,Xu XT,Lyu L,Liu YP,Dimitrov D,Kennedy JD,Wang QG,Tang ZY,Feng XJ(2018)Global patterns of Rhododendron diversity:The role of evolutionary time and diversification rates.Global Ecology and Biogeography,27,913-924.
Song YP,Huang ZH,Huang SQ(2019)Pollen aggregation by viscin threads in Rhododendron varies with pollinator.New Phytologist,221,1150-1159.
Stout JC,Parnell JA,Arroyo J,Crowe TP(2006)Pollination ecology and seed production of Rhododendron ponticum in native and exotic habitats.Biodiversity and Conservation,15,755-777.
Tagane S,Hiramatsu M,Okubo H(2008)Hybridization and asymmetric introgression between Rhododendron eriocarpum and R.indicum on Yakushima Island,southwest Japan.Journal of Plant Research,121,387-395.
Tom E,Ellen DK,Johan VH,Jan DR,Erik VB(2007)Application of embryo rescue after interspecific crosses in the genus Rhododendron.Plant Cell,Tissue and Organ Culture,89,29-35.
Wang DY,Liu YJ,Bai YQ,Xie LJ(2012)The breeding research progress of Rhododendron plants.Journal of Anhui Agriculture Science,40,15622-15625,15627.(in Chinese with English abstract)[王定跃,刘永金,白宇清,谢利娟(2012)杜鹃花属植物育种研究进展.安徽农业科学,40,15622-15625,15627.]
Williams EG,Rouse JL(1988)Lengths contribute to isolation of species in Rhododendron.Australian Journal of Botany,36,183-191.
Williams EG,Rouse JL,Palser BF,Knox RB(1990)Reproductive biology of rhododendron.Horticultural Reviews,12,1-67.
Wolf PG,Doche B,Gielly L,Taberlet P(2004)Genetic structure of Rhododendron ferrugineum at a wide range of spatial scale.Journal of Heredity,95,301-308.
Wu H,Yang XM,Shao HM,Wang F(2013)Germplasm resource base for rhododendron horticulture:Status,problems and countermeasures.Biodiversity Science,21,628-634.(in Chinese with English abstract)[吴荭,杨雪梅,邵慧敏,王飞(2013)杜鹃花产业的种质资源基础:现状、问题与对策.生物多样性,21,628-634.]
Yan LJ,Burgess KS,Milne R,Fu CN,Li DZ,Gao LM(2017)Asymmetrical natural hybridization varies among hybrid swarms between two diploid Rhododendron species.Annals of Botany,120,51-61.
Yan LJ,Burgess KS,Zheng W,Tao ZB,Li DZ,Gao LM(2018)Incomplete reproductive isolation between Rhododendron taxa enables hybrid formation and persistence.Journal of Integrative Plant Biology,61,433-448.
Yu SX(1992)Rhododendron,pp.100-113.Jindun Press,Beijing.(in Chinese)[余树勋(1992)杜鹃花,100-113页.金盾出版社,北京.]
Zha HG,Milne RI,Sun H(2010)Asymmetric hybridization in Rhododendron agastum:A hybrid taxon comprising mainly F1s in Yunnan,China.Annals of Botany,105,89-100.
Zhang CQ,Feng BJ,LüYL(1998)Hybridization study of the genus Rhododendron.Acta Botanica Yunnanica,20,94-96.(in Chinese with English abstract)[张长芹,冯宝钧,吕元林(1998)杜鹃花属植物的杂交研究.云南植物研究,20,94-96.]
Zhang CQ,Luo JF,Feng BJ(2002)New rhododendron hybrid-‘Zhaohui’and‘Hongyun’.Acta Horticulturae Sinica,29,296.(in Chinese with English abstract)[张长芹,罗吉凤,冯宝均(2002)杜鹃花新品种‘朝晖’和‘红晕’.园艺学报,29,296.]
Zhang JL,Zhang CQ,Wu ZQ,Qiao Q(2007)The potential roles of interspecific pollination in natural hybridization of Rhododendron species in Yunnan,China.Biodiversity Science,15,658-665.(in Chinese with English abstract)[张敬丽,张长芹,吴之坤,乔琴(2007)探讨种间传粉在杜鹃花属自然杂交物种形成中的作用.生物多样性,15,658-665.]
Zhang JL,Zhang CQ,Gao LM,Yang JB,Li HT(2007)Natural hybridization origin of Rhododendron agastum(Ericaceae)in Yunnan,China:Inferred from morphological and molecular evidence.Journal of Plant Research,120,457-463.
Zheng SL,Yi CR,Liu Q,Zhang JL(2016)Primary study on hybridization breeding among several Rhododendron species in Yunnan Province.Journal of Yunnan Agricultural University(Natural Science),31,1052-1057.(in Chinese with English abstract)[郑硕理,易陈燃,刘巧,张敬丽(2016)云南几种杜鹃杂交育种初探.云南农业大学学报(自然科学版),31,1052-1057.]
Zhuang P(2012)Discussion on the Rhododendron geographical distribution types and their cause of formation in China.Guihaia,32,150-156.(in Chinese with English abstract)[庄平(2012)中国杜鹃花属植物地理分布型及其成因的探讨.广西植物,32,150-156.]
Zhuang P,Wang F,Shao HM(2013)Comparative study on Rhododendron and their distribution in W-Sichuan and SE-Tibet.Guihaia,33,791-797.(in Chinese with English abstract)[庄平,王飞,邵慧敏(2013)川西与藏东南地区杜鹃花属植物及其分布的比较研究.广西植物,33,791-797.]
Zhuang P(2017a)Natural pollination of 37 Rhododendron species under ex situ conservation.Guihaia,37,947-958.(in Chinese with English abstract)[庄平(2017a)37种杜鹃花属植物在迁地保育下的自然授粉研究.广西植物,37,947-958.]
Zhuang P(2017b)Self-fertilization of 32 Rhododendron species under ex situ conservation.Guihaia,37,959-968.(in Chinese with English abstract)[庄平(2017b)32种杜鹃花属植物在迁地保育条件下的自交研究.广西植物,37,959-968.]
Zhuang P(2018a)Cross fertility of intra-subgen.Hymenanthes of 23 Rhododendron species.Guihaia,38,1545-1557.(in Chinese with English abstract)[庄平(2018a)23种常绿杜鹃亚属植物种间杂交的可育性研究.广西植物,38,1545-1557.]
Zhuang P(2018b)Cross fertility of intra-subgen.Rhododendron of 10 Rhododendron species.Guihaia,38,1558-1565.(in Chinese with English abstract)[庄平(2018b)10种杜鹃亚属植物种间杂交的可育性研究.广西植物,38,1558-1565.]
Zhuang P(2018c)Cross fertility of inter-subgenera of 32Rhododendron species.Guihaia,38,1566-1580.(in Chinese with English abstract)[庄平(2018c)32种杜鹃花属植物亚属间杂交的可育性研究.广西植物,38,1566-1580.]
Zhuang P(2018d)Distribution of hybrid incompatibility and sterility of Rhododendron.Guihaia.38,1581-1587.(in Chinese with English abstract)[庄平(2018d)杜鹃花属植物杂交不亲和与败育分布研究.广西植物,38,1581-1587.]
Zhuang P(2018e)Crossability and its characteristics of Rhododendron.Guihaia.38,1588-1594.(in Chinese with English abstract)[庄平(2018e)杜鹃花属植物种间可交配性及其特点.广西植物,38,1588-1594.]
Zhuang P(2019)Preliminary study on the direction of inter-specific cross of Rhododendron.Guihaia,doi:10.11931/guihaia.gxzw20183003.(in Chinese with English abstract)[庄平(2019)杜鹃花属植物种间杂交向性的初步研究.广西植物,doi:10.11931/guihaia.gxzw20183003.]
Akihide O,Hiroshi I,Kenichi S(2006)Variation in crossability among Rhododendron japonicum f.flavum plants as pollen parents when crossed with evergreen species,R.eriocarpum.Journal of the Japanese Society for Horticultural Science,75,270-272.
Akihide O,Kenichi S(2006)Crossability of Kurume Azaleas as a seed parent for hybridizing with Rhododendron japonicum(A.Gray)J.V.Suringar f.flavum Nakai.Journal of the Japanese Society for Horticultural Science,75,191-198.
Akiko O,Ikumi D,Takashi S(2008)Bumblebee pollination and reproductive biology of Rhododendron semibarbatum(Ericaceae).Journal of Plant Research,121,319-327.
Akira SH(2010)Kinship between parents reduces offspring fitness in a natural population of Rhododendron brachycarpum.Annals of Botany,105,637-646.
Ammal EK(1950)Polyploidy in the genus Rhododendron.The Rhododendron Year Book,5,92-96.
Ammal EK,Enoch IC,Bridgwater M(1950)Chromosome numbers in species of Rhododendron.The Rhododendron Year Book,5,78-91.
Brown GK,Craven LA,Udovicic F,Ladiges PY(2006)Phylogeny of Rhododendron section Vireya(Ericaceae)base on two non-coding regions of cp DNA.Plant Systematics and Evolution,257,57-93.
Chamberlain DF,Hyam R,Argent G,Fairweather G,Walter KS(1996)The Genus Rhododendron,Its Classification and Synonymy.Royal Botanic Garden Edinburgh,Edinburgh.
Escaravage N,Wagner J(2004)Pollination effectiveness and pollen dispersal in a Rhododendron ferrugineum(Ericaceae)population.Plant Biology,6,606-615.
Fang MY,Fang RZ,He MY,Hu LZ,Yang HB(2005)Rhododendron.In:Flora of China(eds Wu ZY,Raven PH,Hong DY),Vol.14,pp.83-87.Science Press,Beijing&Missouri Botanical Garden Press,St.Louis.
Fang RZ,Min TL(1981)The influence of uplift of Himalayas on the floristic formation of genus Rhododendron.Acta Botanica Yunnanica,3,147-157.(in Chinese with English abstract)[方瑞征,闵天禄(1981)喜玛拉雅山脉的隆升对杜鹃属区系形成的影响.云南植物研究,3,147-157.]
Fang RZ,Min TL(1995)The floristic study on the genus Rhododendron.Acta Botanica Yunnanica,17,359-379.(in Chinese with English abstract)[方瑞征,闵天禄(1995)杜鹃属植物区系的研究.云南植物研究,17,359-379.]
Gaku K(1993)Relationships between flowering time and fruit set of the entomophilous alpine shrub,Rhododendron aureum(Ericaceae),inhabiting snow patches.American Journal of Botany,80,1300-1304.
Gaku K,Akira SH,Yuka K(2011)Pollination efficiency of bumblebee queens and workers in the alpine shrub Rhododendron aureum.International Journal of Plant Sciences,172,70-77.
Gao LM,Zhang CQ,Li DZ,Wu D(2005)Chromosome numbers of Rhododendron subgen.Azaleastrum.Acta Botanica Yunnanica,27,433-436.(in Chinese with English abstract)[高连明,张长芹,李德铢,吴丁(2005)杜鹃花属马银花亚属一些种类染色体数目报道.云南植物研究,27,433-436.]
Geng XM,Zhao HJ,Wu YQ,Zhang YM(2017)Cross-compatibility of wild Rhododendron and the effective evaluation indicators.Guihaia,37,979-988.(in Chinese with English abstract)[耿兴敏,赵红娟,吴影倩,张月苗(2017)野生杜鹃杂交亲和性及适宜的评价指标.广西植物,37,979-988.]
Gillian KB,Lyn AC,Frank U,Pauline YL(2006)Phylogenetic relationship of Rhododendron section Vireya(Ericaceae)interred from the ITS nrDNA region.Australian Systematic Botany,19,329-342.
Goetsch L,Eckert AJ,Hall BD(2005)The molecular systematics of Rhododendron(Ericaceae):A phylogeny based upon RPB2 gene sequences.Systematic Botany,30,616-626.
Griffin CAM,Eckert CG(2003)Experimental analysis of biparental inbreeding in a self-fertilizing plant.Evolution,57,1513-1519.
Hirao AS,Kameyama Y,Ohara M,Isagi Y,Kudo G(2006)Seasonal changes in pollinator activities influence pollen dispersal and seed production of the alpine shrub Rhododendron aureum(Ericaceae).Molecular Ecology,15,1165-1173.
Huang CC,Hung KH,Hwang CC(2011)Genetic population structure of the alpine species Rhododendron pseudochrysanthum,sensu lato(Ericaceae)inferred from chloroplast and nuclear DNA.BMC Evolutionary Biology,11,108-124.
Huang ZH,Song YP,Huang SQ(2017)Evidence for passerine bird pollination in Rhododendron species.Ao B Plant,9,1-10.
Jose AM,Juan A,Fernando O(2002)Reproductive ecology of Rhododendron ponticum(Ericaceae)in relict Mediterranean populations.Botanical Journal of the Linnean Society,140,297-311.
Kaori S,Ozaki Y,Ureshino K,Miyajima I,Wakana A,Okubo H(2008)Interploid crossing overcomes plastome-nuclear genome incompatibility in intersubgeneric hybridization between evergreen and deciduous azaleas.Scientia Horticulturae,115,268-274.
Karlsson PS,Jacobson A(2001)Onset of reproduction in Rhododendron lapponicum shoots:The effect of shoot size,age,and nutrient status at two subarctic sites.Oikos,94,279-286.
Kehr AE(1977)Azaleodendron breeding.Journal of American Rhododendron Society,31,226-232.
Kenji U,Miyoko K,Ikuo M(2000)Factors of intersectional unilateral cross incompatibility between several evergreen azalea species and Rhododendron japonicum f.flavum.Journal of the Japanese Society for Horticultural Science,69,261-265.
Kenji U,Yoshiko T,Yuka T,Takeru Ii,Ikue M,Masaru A(2006)Cross compatibility of intersubgeneric hybrids of azaleas on backcross with several evergreen species.Journal of the Japanese Society for Horticultural Science,75,403-409.
Koichi K,Yuji K,Tomohisa Y,Shigeo N,Takashi H(2005)Plastid inheritance and plastome-genome incompatibility of intergeneric hybrids between Menziesia and Rhododendron.Journal of the Japanese Society for Horticultural Science,74,318-323.
Kurashige Y,Etoh JI,Handa T,Takayamagi K,Yukawa T(2001)Section relationships in genus Rhododendron(Ericaceae):Evidence from mat K and trn K intron sequences.Plant Systematic Evolution,228,1-14.
Lan X,Zhang LH,Zhang JZ,Cui HX,Jiang CD,Shi L(2012)Research progress of Rhododendron breeding.Acta Horticulturae Sinica,39,1829-1838.(in Chinese with English abstract)[兰熙,张乐华,张金政,崔红霞,姜闯道,石雷(2012)杜鹃花育种研究进展.园艺学报,39,1829-1838.]
Levins R(1970)Extinction.In:Some Mathematical problems in Biology(ed.Gerstenhaber M),pp.77-107.American Mathematical Society Rhode Island,Providence.
Li SH,Sun WB,Ma YP(2018)Current conservation status and reproductive biology of the giant tree Rhododendron in China.Nordic Journal of Botany,doi:10.1111/njb.01999.
Li XS,Ba CY,Cao HN,Zong CG,Qu L,Zhang JZ(2015)Interspecific cross compatibility of Rhododendron in Changbai Mountain.Agricultural Science&Techno,16,1617-1620.
Liu DF,Xue YB(1997)Plant self-incompatibility.In:Genetics of Plant Reproduction(ed.Meng JL),pp.214-277.Science Press,Beijing.(in Chinese)[刘定富,薛永彪(1997)植物自交不亲和性,214-277页.见:植物生殖遗传学(孟金陵主编).科学出版社,北京.]
Liu XQ,Su JL,Li C,Liu XH(2010)Study on the fruitfulness of hybridization,selfing and open pollination of Rhododendron.Acta Agriculture Shanghai,26,145-148.(in Chinese with English abstract)[刘晓青,苏家乐,李畅,刘晓宏(2010)杜鹃花自交、杂交及开放授粉结实性研究.上海农业学报,26,145-148.]
Liu YF,Xing M,Zhao W,Fan RY,Luo S,Chen X(2012)Genetic diversity analysis of Rhododendron aureum Georgi(Ericaceae)located on Changbai Mountain using ISSR and RAPD markers.Plant Systematic Evolution,298,921-930.
Ma YP,Zhang CQ,Zhang JL,Yang YB(2010)Natural hybridization between Rhododendron delavayi and R.cyanocarpum(Ericaceae),from morphological,molecular and reproductive evidence.Journal of Integrative Plant Biology,52,844-851.
Ma YP,Xie WJ,Sun WB,Marczewski T(2016)Strong reproductive isolation despite occasional hybridization between a widely distributed and a narrow endemic Rhododendron species.Scientific Reports,6,19146.
Meng JL(1997)Genetics of Plant Reproduction,pp.296-357.Science Press,Beijing.(in Chinese)[孟金陵(1997)植物生殖遗传学,296-357页.科学出版社,北京.]
Milne RI,Terzioglu S,Abbott RJ(2003)A hybrid zone dominated by fertile F1s:Maintenance of species barriers in Rhododendron.Molecular Ecology,12,2719-2729.
Milne RI,Davies C,Prickett R,Inns LH(2010)Phylogeny of Rhododendron subgenus Hymenanthes based on chloroplast DNA markers:Between-lineage hybridisation during adaptive radiation?Plant Systematic Evolution,285,233-244.
Min TL,Fang RZ(1979)On the origin and geographic distribution of genus Rhododendron L.Acta Botanica Yunnanica,1,121-127.(in Chinese with English abstract)[闵天禄,方瑞征(1979)杜鹃属Rhododendron L.的地理分布及起源问题的探讨.云南植物研究,1,121-127.]
Min TL,Fang RZ(1990)The phylogeny and evolution of genus Rhododendron.Acta Botanica Yunnanica,12,353-365.(in Chinese with English abstract)[闵天禄,方瑞征(1990)杜鹃属的系统发育与进化.云南植物研究,12,353-365.]
Nathannel TW,Erin ED,Joseph BF,Stefan HG,David AM,Justin GS,Timothy MS,Sarah LR,Andrew GZ(2006)Pollinator limitation,autogamy and minimal inbreeding depression in insect-pollinated plants on a boreal island.American Midland Naturalist,155,19-38.
Ng SC,Corlett RT(2000)Comparative reproductive biology of the six species of Rhododendron(Ericaceae)in Hong Kong,South China.Canadian Journal of Botany,78,221-229.
Nobuo K,Daiki M,Akira N,Masaru A(2008)Attaining inter-subgeneric hybrids in fragrant azalea breeding and the inheritance of organelle DNA.Euphytica,159,67-72.
Oetsch LG,Eckert AJ,Hall BD(2005)The molecular systematics of Rhododendron(Ericaceae):A phylogeny based upon RPB2 gene sequences.Systematic Botany,30,616-626.
Palser BF,Rouse JL and Williams EG(1989)Coordinated timetables for megagametophyte development and pollen tube growth in Rhododendron nuttallii from anthesis to early postfertilization.American Journal of Botany,76,1167-1202.
Pierce L(1974)An unusual intergeneric cross.Journal of American Rhododendron Society,28,45-50.
Pornon A,Escaravage N,Boche TB(1997)Variation of reproductive traits in Rhododendron ferrugineum L.(Ericaceae)population along a successional gradient.Plant Ecology,130,1-11.
Richard JA,Juliet KJ,Richard IM,Amanda CM(2003)Plant introduction,hybridization and gene flow.Philosophical Transactions of the Royal Society B:Biological Sciences,358,1123-1132.
Rouse JL,Knox RB,Williams EG(1993)Inter-and intraspecific pollinations involving Rhododendron species.Journal of American Rhododendron Society,47,23-28.
Ruth A,Kwiton J,George A(2000)Chromosome numbers of some tropical Rhdodendrons(Section Vireya).Edinburgh Journal of Botany,57,1-7.
Sax K(1930)Chromosome stability in the genus Rhododendron.American Journal of Botany,17,247-251.
Schepper SD,Leus L,Mertens M,Bockstaele EV,Loose MD,Debergh P,Heursel J(2001)Flow cytometric analysis of ploidy in Rhododendron(subgenus Tsutsusi).Hortscience,36,125-127.
Shrestha,N,Wang ZH,Su XY,Xu XT,Lyu L,Liu YP,Dimitrov D,Kennedy JD,Wang QG,Tang ZY,Feng XJ(2018)Global patterns of Rhododendron diversity:The role of evolutionary time and diversification rates.Global Ecology and Biogeography,27,913-924.
Song YP,Huang ZH,Huang SQ(2019)Pollen aggregation by viscin threads in Rhododendron varies with pollinator.New Phytologist,221,1150-1159.
Stout JC,Parnell JA,Arroyo J,Crowe TP(2006)Pollination ecology and seed production of Rhododendron ponticum in native and exotic habitats.Biodiversity and Conservation,15,755-777.
Tagane S,Hiramatsu M,Okubo H(2008)Hybridization and asymmetric introgression between Rhododendron eriocarpum and R.indicum on Yakushima Island,southwest Japan.Journal of Plant Research,121,387-395.
Tom E,Ellen DK,Johan VH,Jan DR,Erik VB(2007)Application of embryo rescue after interspecific crosses in the genus Rhododendron.Plant Cell,Tissue and Organ Culture,89,29-35.
Wang DY,Liu YJ,Bai YQ,Xie LJ(2012)The breeding research progress of Rhododendron plants.Journal of Anhui Agriculture Science,40,15622-15625,15627.(in Chinese with English abstract)[王定跃,刘永金,白宇清,谢利娟(2012)杜鹃花属植物育种研究进展.安徽农业科学,40,15622-15625,15627.]
Williams EG,Rouse JL(1988)Lengths contribute to isolation of species in Rhododendron.Australian Journal of Botany,36,183-191.
Williams EG,Rouse JL,Palser BF,Knox RB(1990)Reproductive biology of rhododendron.Horticultural Reviews,12,1-67.
Wolf PG,Doche B,Gielly L,Taberlet P(2004)Genetic structure of Rhododendron ferrugineum at a wide range of spatial scale.Journal of Heredity,95,301-308.
Wu H,Yang XM,Shao HM,Wang F(2013)Germplasm resource base for rhododendron horticulture:Status,problems and countermeasures.Biodiversity Science,21,628-634.(in Chinese with English abstract)[吴荭,杨雪梅,邵慧敏,王飞(2013)杜鹃花产业的种质资源基础:现状、问题与对策.生物多样性,21,628-634.]
Yan LJ,Burgess KS,Milne R,Fu CN,Li DZ,Gao LM(2017)Asymmetrical natural hybridization varies among hybrid swarms between two diploid Rhododendron species.Annals of Botany,120,51-61.
Yan LJ,Burgess KS,Zheng W,Tao ZB,Li DZ,Gao LM(2018)Incomplete reproductive isolation between Rhododendron taxa enables hybrid formation and persistence.Journal of Integrative Plant Biology,61,433-448.
Yu SX(1992)Rhododendron,pp.100-113.Jindun Press,Beijing.(in Chinese)[余树勋(1992)杜鹃花,100-113页.金盾出版社,北京.]
Zha HG,Milne RI,Sun H(2010)Asymmetric hybridization in Rhododendron agastum:A hybrid taxon comprising mainly F1s in Yunnan,China.Annals of Botany,105,89-100.
Zhang CQ,Feng BJ,LüYL(1998)Hybridization study of the genus Rhododendron.Acta Botanica Yunnanica,20,94-96.(in Chinese with English abstract)[张长芹,冯宝钧,吕元林(1998)杜鹃花属植物的杂交研究.云南植物研究,20,94-96.]
Zhang CQ,Luo JF,Feng BJ(2002)New rhododendron hybrid-‘Zhaohui’and‘Hongyun’.Acta Horticulturae Sinica,29,296.(in Chinese with English abstract)[张长芹,罗吉凤,冯宝均(2002)杜鹃花新品种‘朝晖’和‘红晕’.园艺学报,29,296.]
Zhang JL,Zhang CQ,Wu ZQ,Qiao Q(2007)The potential roles of interspecific pollination in natural hybridization of Rhododendron species in Yunnan,China.Biodiversity Science,15,658-665.(in Chinese with English abstract)[张敬丽,张长芹,吴之坤,乔琴(2007)探讨种间传粉在杜鹃花属自然杂交物种形成中的作用.生物多样性,15,658-665.]
Zhang JL,Zhang CQ,Gao LM,Yang JB,Li HT(2007)Natural hybridization origin of Rhododendron agastum(Ericaceae)in Yunnan,China:Inferred from morphological and molecular evidence.Journal of Plant Research,120,457-463.
Zheng SL,Yi CR,Liu Q,Zhang JL(2016)Primary study on hybridization breeding among several Rhododendron species in Yunnan Province.Journal of Yunnan Agricultural University(Natural Science),31,1052-1057.(in Chinese with English abstract)[郑硕理,易陈燃,刘巧,张敬丽(2016)云南几种杜鹃杂交育种初探.云南农业大学学报(自然科学版),31,1052-1057.]
Zhuang P(2012)Discussion on the Rhododendron geographical distribution types and their cause of formation in China.Guihaia,32,150-156.(in Chinese with English abstract)[庄平(2012)中国杜鹃花属植物地理分布型及其成因的探讨.广西植物,32,150-156.]
Zhuang P,Wang F,Shao HM(2013)Comparative study on Rhododendron and their distribution in W-Sichuan and SE-Tibet.Guihaia,33,791-797.(in Chinese with English abstract)[庄平,王飞,邵慧敏(2013)川西与藏东南地区杜鹃花属植物及其分布的比较研究.广西植物,33,791-797.]
Zhuang P(2017a)Natural pollination of 37 Rhododendron species under ex situ conservation.Guihaia,37,947-958.(in Chinese with English abstract)[庄平(2017a)37种杜鹃花属植物在迁地保育下的自然授粉研究.广西植物,37,947-958.]
Zhuang P(2017b)Self-fertilization of 32 Rhododendron species under ex situ conservation.Guihaia,37,959-968.(in Chinese with English abstract)[庄平(2017b)32种杜鹃花属植物在迁地保育条件下的自交研究.广西植物,37,959-968.]
Zhuang P(2018a)Cross fertility of intra-subgen.Hymenanthes of 23 Rhododendron species.Guihaia,38,1545-1557.(in Chinese with English abstract)[庄平(2018a)23种常绿杜鹃亚属植物种间杂交的可育性研究.广西植物,38,1545-1557.]
Zhuang P(2018b)Cross fertility of intra-subgen.Rhododendron of 10 Rhododendron species.Guihaia,38,1558-1565.(in Chinese with English abstract)[庄平(2018b)10种杜鹃亚属植物种间杂交的可育性研究.广西植物,38,1558-1565.]
Zhuang P(2018c)Cross fertility of inter-subgenera of 32Rhododendron species.Guihaia,38,1566-1580.(in Chinese with English abstract)[庄平(2018c)32种杜鹃花属植物亚属间杂交的可育性研究.广西植物,38,1566-1580.]
Zhuang P(2018d)Distribution of hybrid incompatibility and sterility of Rhododendron.Guihaia.38,1581-1587.(in Chinese with English abstract)[庄平(2018d)杜鹃花属植物杂交不亲和与败育分布研究.广西植物,38,1581-1587.]
Zhuang P(2018e)Crossability and its characteristics of Rhododendron.Guihaia.38,1588-1594.(in Chinese with English abstract)[庄平(2018e)杜鹃花属植物种间可交配性及其特点.广西植物,38,1588-1594.]
Zhuang P(2019)Preliminary study on the direction of inter-specific cross of Rhododendron.Guihaia,doi:10.11931/guihaia.gxzw20183003.(in Chinese with English abstract)[庄平(2019)杜鹃花属植物种间杂交向性的初步研究.广西植物,doi:10.11931/guihaia.gxzw20183003.]