谷氨酰胺对22～28日龄热应激肉鸡骨骼肌氧化损伤的改善作用
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摘要
本试验旨在研究谷氨酰胺(Gln)对热应激肉鸡骨骼肌抗氧化能力的影响。选用300只22日龄AA肉鸡,随机分为5个处理组:常温对照组和4个热应激组(基础日粮分别添加0%、0.5%、1%和1.5%Gln),试验期为22～28日龄。结果表明:与对照组相比,热应激组肉鸡胸肌和腿肌丙二醛(MDA)含量显著升高,胸肌抗超氧阴离子能力、总抗氧化能力(T-AOC)、谷胱甘肽(GSH)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和谷胱甘肽巯基转移酶(GST)显著降低,腿肌GSH和SOD显著降低(P<0.05)。与热应激组相比,日粮添加1.5%Gln显著降低肉鸡胸肌和腿肌MDA含量,胸肌T-AOC、GSH、CAT和GST显著升高,腿肌GSH和SOD含量显著升高(P<0.05)。Gln能缓解热应激肉鸡的肌肉氧化损伤,提高肌肉抗氧化能力。
This study was conducted to explore the effect of glutamine(Gln) on broiler skeletal muscle antioxidative capability under heat stress condition.300 22-day-old AA broilers were randomly divided into five treatment groups. The 5 groups included the normal temperature control group, and four heat stress groups(basic diet supplemented with 0%, 0.5%, 1%, and 1.5% Gln).The experiment lasted for 7 days(22～28 days). Compared with the control group, the MDA content of broiler breast and thigh muscles in heat stress group was significantly increased; the anti-superoxideanion ability, total antioxidant capacity(T-AOC), glutathione(GSH), catalase(CAT), superoxide dismutase(SOD), and glutathione S-transferase(GST) levels of breast muscle were significantly decreased; the activities of GSH and SOD in thigh muscle were significantly decreased(P<0.05). Compared with heat stress group, the diet supplemented with 1.5%Gln significantly decreased the content of MDA in broiler breast and thigh muscles; the activities of T-AOC, GSH, CAT, and GST in breast muscle were significantly increased; the activities of GSH and SOD in thigh muscle were significantly increased(P<0.05). These results suggested that Gln was capable of alleviating oxidative stress induced by heat stress in broiler muscle.
This study was conducted to explore the effect of glutamine(Gln) on broiler skeletal muscle antioxidative capability under heat stress condition.300 22-day-old AA broilers were randomly divided into five treatment groups. The 5 groups included the normal temperature control group, and four heat stress groups(basic diet supplemented with 0%, 0.5%, 1%, and 1.5% Gln).The experiment lasted for 7 days(22～28 days). Compared with the control group, the MDA content of broiler breast and thigh muscles in heat stress group was significantly increased; the anti-superoxideanion ability, total antioxidant capacity(T-AOC), glutathione(GSH), catalase(CAT), superoxide dismutase(SOD), and glutathione S-transferase(GST) levels of breast muscle were significantly decreased; the activities of GSH and SOD in thigh muscle were significantly decreased(P<0.05). Compared with heat stress group, the diet supplemented with 1.5%Gln significantly decreased the content of MDA in broiler breast and thigh muscles; the activities of T-AOC, GSH, CAT, and GST in breast muscle were significantly increased; the activities of GSH and SOD in thigh muscle were significantly increased(P<0.05). These results suggested that Gln was capable of alleviating oxidative stress induced by heat stress in broiler muscle.
引文
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[9]ZHANG J, HU Z, LU C, et al. Effect of various levels of dietary curcumin on meat quality and antioxidant profile of breast muscle in broilers[J]. Journal of Agricultural and Food Chemistry, 2015,63(15):3880-3886
[10]李叶涵,周佳,张越,等.L-精氨酸和α-酮戊二酸对热应激肉鸡肝脏功能的影响[J].饲料工业,2016,37(16):6-11LI Y H, ZHOU J, ZHANG Y, et al.Effects of L-arginine andα-ketoglutarate on liver function of heat-stressed broilers[J].Feed Industry, 2016,37(16):6-11
[11]MA X, LIN Y, ZHANG H, et al. Heat stress impairs the nutritional metabolism and reduces the productivity of egglaying ducks[J]. Animal Reproduction Science,2014, 145(3):182-190
[12]杨小婷,李吕木.谷氨酰胺在肉鸡生产中的研究进展[J].中国饲料,2012(1):15-17YANG X T,LI L M. Research progress of glutamine in broiler production[J].China Feed, 2012(1):15-17
[13]李文立,路静,孙振钧,等.谷氨酰胺对热应激肉鸡抗氧化性能的影响[J].动物营养学报,2011, 23(4):695-702LI W L, LU J, SUN Z J, et al.Effect of glutamine on antioxidant function of broilers under heatstress[J]. Chinese Journal of Animal Nutrition, 2011, 23(4):695-702
[14]PAMOK S, AENGWANICH W, KOMUTRIN T. Adaptation to oxidative stress and impact of chronic oxidative stress on immunity in heat-stressed broilers[J]. Journal of Thermal Biology, 2009, 34(7):353-357
[15]MARQUES C, LICKS F, ZATTONI I, et al.Antioxidant properties of glutamine and its role in VEGF-Akt pathways in portal hypertension gastropathy[J]. World Journal of Gastroenterology:WJG, 2013, 19(28):4464
[16]唐现文,刘勇.谷氨酰胺对热应激肉仔鸡生长性能及抗氧化活性的影响[J].黑龙江畜牧兽医,2012(12):51-53TANG X W, XIAN Y.Effects of glutamine on growth performance and antioxidant activity of heat stressed broilers[J].Heilongjiang Animal Science and Veterinary Medicine,2012(12):51-53.
[2]MUJAHID A, PUMFORD N R, BOTTJE W, et al. Mitochondrial oxidative damage in chicken skeletal muscle induced by acute heat stress[J]. The Journal of Poultry Science, 2007,44(4):439-445
[3]HEYLAND D K, ELKE G, COOK D, et al. Glutamine and antioxidants in the critically ill patient:a post hoc analysis of a large-scale randomized trial[J]. Journal of Parenteral and Enteral Nutrition, 2015, 39(4):401-409
[4]HU H, BAI X, SHAH A A, et al. Dietary supplementation with glutamine andγ-aminobutyricacid improves growth performance and serum parameters in 22-to 35-day-old broilers exposed to hot environment[J]. Journal of Animal physiology and Animal Nutrition, 2016, 100(2):361-370
[5]OLUBODUN J O, ZULKIFLI I, FARJAM A S, et al. Glutamine and glutamic acid supplementation enhances performance of broiler chickens under the hot and humid tropical condition[J]. Italian Journal of Animal Science, 2015, 14(1):3263
[6]HU H, BAI X, WEN A, et al. Assessment of interactions between glutamine and glucose on meat quality,AMPK,and glutamine concentrations in pectoralis major meat of broilers under acute heat stress[J].Journal of Applied Poultry Research, 2016, 25(3):370-378
[7]HABIBIAN M, GHAZI S, MOEINI M M, et al. Effects ofdietary selenium and vitamin E on immune response and biological blood parameters of broilers reared under thermoneutral or heat stress conditions[J]. International Journal of Biometeorology, 2014,58(5):741-752
[8]RIMOLDI S, LASAGNA E, SARTI F M, et al. Expression profile of six stress-related genes and productive performances of fast and slow growing broiler strains reared under heat stress conditions[J]. Meta Gene,2015, 6:17-25
[9]ZHANG J, HU Z, LU C, et al. Effect of various levels of dietary curcumin on meat quality and antioxidant profile of breast muscle in broilers[J]. Journal of Agricultural and Food Chemistry, 2015,63(15):3880-3886
[10]李叶涵,周佳,张越,等.L-精氨酸和α-酮戊二酸对热应激肉鸡肝脏功能的影响[J].饲料工业,2016,37(16):6-11LI Y H, ZHOU J, ZHANG Y, et al.Effects of L-arginine andα-ketoglutarate on liver function of heat-stressed broilers[J].Feed Industry, 2016,37(16):6-11
[11]MA X, LIN Y, ZHANG H, et al. Heat stress impairs the nutritional metabolism and reduces the productivity of egglaying ducks[J]. Animal Reproduction Science,2014, 145(3):182-190
[12]杨小婷,李吕木.谷氨酰胺在肉鸡生产中的研究进展[J].中国饲料,2012(1):15-17YANG X T,LI L M. Research progress of glutamine in broiler production[J].China Feed, 2012(1):15-17
[13]李文立,路静,孙振钧,等.谷氨酰胺对热应激肉鸡抗氧化性能的影响[J].动物营养学报,2011, 23(4):695-702LI W L, LU J, SUN Z J, et al.Effect of glutamine on antioxidant function of broilers under heatstress[J]. Chinese Journal of Animal Nutrition, 2011, 23(4):695-702
[14]PAMOK S, AENGWANICH W, KOMUTRIN T. Adaptation to oxidative stress and impact of chronic oxidative stress on immunity in heat-stressed broilers[J]. Journal of Thermal Biology, 2009, 34(7):353-357
[15]MARQUES C, LICKS F, ZATTONI I, et al.Antioxidant properties of glutamine and its role in VEGF-Akt pathways in portal hypertension gastropathy[J]. World Journal of Gastroenterology:WJG, 2013, 19(28):4464
[16]唐现文,刘勇.谷氨酰胺对热应激肉仔鸡生长性能及抗氧化活性的影响[J].黑龙江畜牧兽医,2012(12):51-53TANG X W, XIAN Y.Effects of glutamine on growth performance and antioxidant activity of heat stressed broilers[J].Heilongjiang Animal Science and Veterinary Medicine,2012(12):51-53.