辣椒应答冷信号转导机制研究进展
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摘要
辣椒原产中南美洲热带地区,为喜温性蔬菜作物。冷害是辣椒生产中的主要逆境因子,严重影响辣椒的生长发育。遭受冷害胁迫时,包括辣椒在内的众多植物通过多个信号转导途径调节耐冷相关基因的表达,提高植物的耐冷性。本文从冷信号感知、冷信号转导途径中关键转录因子、耐冷基因表达等方面,综述了辣椒应答冷信号的研究进展,从而为辣椒耐冷分子机制研究提供参考。
Pepper(Capsicum spp.), which originates from tropical region of Central and South America, is a thermophilic vegetable crop. Cold stress is one of the main environmental limitations in pepper production, which seviously affects plant growth and development. Under chilling stress, many plants, including pepper, regulate the expression of genes related to cold tolerance various signal transduction pathways to improve cold tolerance. In this paper, the research progress of response to cold stress in pepper was reviewed from the aspects of cold signal sensing, the key transcription factors and expression of coldtolerance genes in cold-related signal transduction pathways. This paper provides reference for the study of molecular mechanism of cold tolerance in pepper.
Pepper(Capsicum spp.), which originates from tropical region of Central and South America, is a thermophilic vegetable crop. Cold stress is one of the main environmental limitations in pepper production, which seviously affects plant growth and development. Under chilling stress, many plants, including pepper, regulate the expression of genes related to cold tolerance various signal transduction pathways to improve cold tolerance. In this paper, the research progress of response to cold stress in pepper was reviewed from the aspects of cold signal sensing, the key transcription factors and expression of coldtolerance genes in cold-related signal transduction pathways. This paper provides reference for the study of molecular mechanism of cold tolerance in pepper.
引文
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[2] 刘慧英,王祯丽,王玉华.不同品种辣椒种子发芽和苗期耐冷性差异的研究[J].石河子大学学报(自然科学版),2002,6(1):23-26.
[3] 杨万基,蒋欣梅,高欢,等.28-高芸苔素内酯对低温弱光胁迫辣椒幼苗光合和荧光特性的影响[J].南方农业学报,2018,49(4):741-747.
[4] TARCHOUN N,M'HAMDI M,SILVA J A T D.Approaches to evaluate the abortion of hot pepper floral structures induced by low night temperature[J].Europ J Hort Sci,2012,77(2):78-83.
[5] MERCADO J A,REID M S,VALPUESTA V,et al.Metabolic changes and susceptibility to chilling stress in Capsicum annuum plants grown at suboptimal temperature[J].Aust J Plant Physiol,1997,24:759-767.
[6] 王立浩,张正海,曹亚从,等.“十二五”我国辣椒遗传育种研究进展及其展望[J].中国蔬菜,2016 (1):1-7.
[7] 耿三省,陈斌,张晓芬,等.我国辣椒品种市场需求变化趋势及育种对策[J].中国蔬菜,2015(3):1-5.
[8] 陈斌,张晓芬,杜和山,等.辣椒新品种胜寒740的选育[J].中国蔬菜,2018 (8):70-72.
[9] THOMASHOW M F.Plant cold acclimation:freezing tolerance genes and regulatory mechanisms[J].Annu Rev Plant Biol,1999,50:571-599.
[10] STEPONKUS P L.Role of the plasma membrane in freezing injury and cold acclimation[J].Annu Rev Plant Physiol,1984,35:543-584.
[11] WANG D Z,JIN Y N,DING X H,et al.Gene regulation and signal transduction in the ICE-CBF-COR signaling pathway during cold stress in plants[J].Biochemistry,2017,82(10):1103-1117.
[12] THOMASHOW M F.Molecular basis of plant cold acclimation:insights gained from studying the CBF cold response pathway[J].Plant Physiol,2010,154:571-577.
[13] JANMOHAMMADI M,ZOLLA L,RINALDUCCI S.Low temperature tolerance in plants:changes at the protein level[J].Phytochemistry,2015,117:76-89.
[14] MA Y,DAI X,XU Y,et al.COLD1 confers chilling tolerance in rice[J].Cell,2015,160:1209-1221.
[15] YANG T,POOVAIAH B W.Calcium/calmodulin-mediated signal network in plants[J].Trends Plant Sci,2003,8(10):505-512.
[16] 张化生,郭晓冬,王萍.低温胁迫下硝酸钙处理对辣椒幼苗抗冷性的影响[J].甘肃农业大学学报,2008,43(2):66-69.
[17] 武丽丽,张国斌,郁继华.CaCl2与SA对辣椒幼苗抗冷性的影响[J].甘肃农业大学学报,2009,44(6):44-47.
[18] 郭晓冬,邹志荣,张化生.硝酸钙浸种对低温下辣椒幼苗渗透调节物质的影响[J].干旱地区农业研究,2008,26(5):160-164.
[19] DING Y,LI H,ZHANG X,et al.OST1 kinase modulates freezing tolerance by enhancing ICE1 stability in Arabidopsis[J].Dev Cell,2015,32:278-289.
[20] PARK S Y,FUNG P,NISHIMURA N,et al.Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of START proteins[J].Science,2009,324:1068-1071.
[21] 任旭琴.低温解除后辣椒叶片中内源激素的动态变化研究[J].安徽农业大学学报,2013,40(5):867-870.
[22] 汤日圣,黄益洪,唐现洪,等.微生物源脱落酸(ABA)对辣椒苗耐冷性的影响[J].江苏农业学报,2008,24(4):467-470.
[23] 罗立津,徐福乐,翁华钦,等.脱落酸对甜椒幼苗抗寒性的诱导效应及其机理研究[J].西北植物学报,2011,31(1):94-100.
[24] 张国斌,郁继华,冯致,等.NO和ABA对辣椒幼苗自毒作用缓解的生理生化机制[J].园艺学报,2013,40(3):458-466.
[25] MEDINA J,BARGUES M,TEROL J,et al.The Arabidopsis CBF gene family is composed of three genes encoding AP2 domain-containing proteins whose expression is regulated by low temperature but not by abscisic acid or dehydration[J].Plant Physiol,1999,119:463-469.
[26] GILMOUR S J,SEBOLT A M,SALAZAR M P,et al.Overexpression of the Arabidopsis CBF3 transcriptional activator mimics multiple biochemical changes associated with cold acclimation[J].Plant Physiol,2000,124:1854-1865.
[27] STOCKINGER E J,GILMOUR S J,THOMASHOW M F.Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE,a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit[J].Proc Natl Acad Sci USA,1997,94:1035-1040.
[28] HSIEH T H,LEE J T,CHARNG Y Y,et al.Tomato plants ectopically expressing Arabidopsis CBF1 show enhanced resistance to water deficit stress[J].Plant Physiol,2002,130:618-626.
[29] DOU H,XV K,MENG Q,et al.Potato plants ectopically expressing Arabidopsis thaliana CBF3 exhibit enhanced tolerance to high-temperature stress[J].Plant Cell Environ,2014,38:61-72.
[30] HAAKE V,COOK D,RIECHMANN J L,et al.Transcription factor CBF4 is a regulator of drought adaptation in Arabidopsis[J].Plant Physiol,2002,130:639-648.
[31] 王兴娥,巩振辉,李大伟,等.冷诱导基因C-重复基序结合因子4(CBF4)在辣椒中的遗传转化及抗寒性分析[J].农业生物技术学报,2009,17(5):830-835.
[32] KIM S,AN C S,HONG Y N,et al.Cold-inducible transcription factor,CaCBF,is associated with a homeodomain leucine zipper protein in hot pepper (Capsicum annuum L.)[J].Mol Cells,2004,18(3):300-308.
[33] YANG S,TANG X F,MA N N,et al.Heterology expression of the sweet pepper CBF3 gene confers elevated tolerance to chilling stress in transgenic tobacco[J].J Plant Physiol,2011,168:1804-1812.
[34] HONG J P,KIM W T.Isolation and functional characterization of the Ca-DREBLP1 gene encoding a dehydration-responsive element binding-factor-like protein 1 in hot pepper (Capsicum annuum L.cv.Pukang)[J].Planta,2005,220:875-888.
[35] EULGEM T,RUSHTON P J,ROBATZEK S,et al.The WRKY superfamily of plant transcription factors[J].Trends Plant Sci,2000,5(5):199-206.
[36] ZOU C,JIANG W,YU D.Male gametophyte-specific WRKY34 transcription factor mediates cold sensitivity of mature pollen in Arabidopsis[J].J Exp Bot,2010,61(14):3901-3914.
[37] 刁卫平,王述彬,刘金兵,等.辣椒全基因组WRKY转录因子的分析[J].园艺学报,2015,42(11):2183-2196.
[38] MOON S J,HAN S Y,KIM D Y,et al.Ectopic expression of CaWRKY1,a pepper transcription factor,enhances drought tolerance in transgenic potato plants[J].J Plant Biol,2014,57:198-207.
[39] YU B K,LEE J H,SHIN S J,et al.Molecular characterization of cold stress-related transcription factors,CaEREBP-C1,-C2,-C3,and CaWRKY1A from Capsicum annuum L.[J].J Plant Biol,2013,56:106-114.
[40] OOKA H,SATOH K,DOI K,et al.Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis thaliana[J].DNA Res,2003,10(6):239-247.
[41] CHANGSONG Z,DIQIU Y.Analysis of the cold-responsive transcriptome in the mature pollen of Arabidopsis[J].J Plant Biol,2010,53:400-416.
[42] MA N N,ZUO Y Q,LIANG X Q,et al.The multiple stress-responsive transcription factor SlNAC1 improves the chilling tolerance of tomato[J].Physiol Plantarum,2013,149(4):474-486.
[43] LI X D,ZHUANG K Y,LIU Z M,et al.Overexpression of a novel NAC-type tomato transcription factor,SlNAM1,enhances the chilling stress tolerance of transgenic tobacco[J].J Plant Physiol,2016,204:54-65.
[44] DIAO W,SNYDER J C,WANG S,et al.Genome-wide analyses of the NAC transcription factor gene family in pepper (Capsicum annuum L.):chromosome location,phylogeny,structure,expression patterns,cis-elements in the promoter,and interaction network[J].Int J Mol Sci,2018,19(4):1048-1061.
[45] GUO W L,WANG S B,CHEN R G,et al.Characterization and expression profile of CaNAC2 pepper gene[J].Front Plant Sci,2015,6:755.
[46] JIN H,MARTIN C.Multifunctionality and diversity within the plant MYB-gene family[J].Plant Mol Biol,1999,41:577-585.
[47] DUBOS C,STRACKE R,GROTEWOLD E,et al.MYB transcription factors in Arabidopsis[J].Trends Plant Sci,2010,15(10):573-581.
[48] AGARWAL M,HAO Y,KAPOOR A,et al.A R2R3 type MYB transcription factor is involved in the cold regulation of CBF genes and in acquired freezing tolerance[J].J Biol Chem,2006,281(49):37636-37645.
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