生长激素受体基因在美国短毛黑水貂组织中表达量变化研究
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摘要
为了探讨水貂生长激素受体(GHR)基因在不同组织中的表达变化,以美国短毛黑水貂为研究对象,采用荧光定量PCR方法检测了GHR基因在不同生长发育阶段(45、90、120、150、180日龄)心脏、肝脏、脾脏、肺脏、肾脏、肠、皮肤等组织的表达水平,并进行了比较分析。结果表明:GHR基因在同一组织不同日龄表达具有较大差异,例如,在心脏、肝脏和脾脏组织,180日龄表达量极显著高于其他日龄(P<0.01),而肠组织,其在45、120日龄均极显著高于其他日龄(P<0.01);此外GHR表达量在同一日龄不同组织同样存在较大差异,例如,90日龄时皮肤组织极显著高于其他组织(P<0.01),180日龄时脾脏极显著高于其他组织(P<0.01)。说明水貂GHR基因的表达呈现出明显的时空特异性。
To explore the expression changes of Growth Hormane Recerfor(GHR) gene in different tissues of mink,real-time PCR was conducted to quantify and compare the expression of GHR gene in heart, liver, spleen, lung, kidney, intestine and skin tissue of American mink during different growth stages(at the age of 45, 90, 120, 150 and 180 days old). The results showed that GHR gene in the same tissue had different expression level at different ages. For instance, the expression level of GHR gene in heart, liver and spleen at 180 days old was significantly higher than that at the others(P<0.01). Furthermore, the expression level in intestine at 45 and 120 days old were significantly higher than the others separately(P<0.01). On the other side, GHR gene in different tissues also had different expression level at the same age. For example, the expression level of GHR gene in skin tissue was significantly higher than that in other tissues at the 90 days old(P<0.01), and in spleen tissue, the expression level of GHR gene was significantly higher than in other tissues at 180 days old(P<0.01). It was concluded that the expression of GHR was of significantly obvious spatio-temporal specificity, which could provide a theoretical basis for exploring the regulation mechanism of growth and development in mink.
To explore the expression changes of Growth Hormane Recerfor(GHR) gene in different tissues of mink,real-time PCR was conducted to quantify and compare the expression of GHR gene in heart, liver, spleen, lung, kidney, intestine and skin tissue of American mink during different growth stages(at the age of 45, 90, 120, 150 and 180 days old). The results showed that GHR gene in the same tissue had different expression level at different ages. For instance, the expression level of GHR gene in heart, liver and spleen at 180 days old was significantly higher than that at the others(P<0.01). Furthermore, the expression level in intestine at 45 and 120 days old were significantly higher than the others separately(P<0.01). On the other side, GHR gene in different tissues also had different expression level at the same age. For example, the expression level of GHR gene in skin tissue was significantly higher than that in other tissues at the 90 days old(P<0.01), and in spleen tissue, the expression level of GHR gene was significantly higher than in other tissues at 180 days old(P<0.01). It was concluded that the expression of GHR was of significantly obvious spatio-temporal specificity, which could provide a theoretical basis for exploring the regulation mechanism of growth and development in mink.
引文
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[2] 程胜利,杨保平,肖玉萍等.生长激素受体基因的研究进展[J].中国草食动物科学,2013,33(2):62.
[3] 赵茹茜.鸡生长激素受体研究的新进展[J].中国家禽,1999,21(6):41-42.
[4] GODOWSHI P J,LEUNG D W,MEACHAM I R,et al.Characterization of the human growth hormone receptor gene and demonstration of a partial gene deletion in two patients with Laron-type dwarfism[J].Proc Natl Acad Sci USA,1989,86(20):8 083-8 087.
[5] ARGETSINGER L S,CARTER-SU C.Mechanism of signaling by growth hormone receptor[J].Physiol Rev,1996,76(4):1 089-1 107.
[6] 孙逊,朱尚权.生长激素受体的结构、功能及其信号转导[J].国外医学(生理病理科学与临床分册),1999,19(1):9-14.
[7] BOCHAROV EV,LESOVOY DM,BOCHAROVA OV,et al.Structural basis of the signal transduction via transmembrance domain of the human growth hormone receptor[J].Biochim Biophys Acta,2018,1862(6):1 410-1 420.
[8] KOEPFLI K P,WAYNE R K.Type I STS markers are more informative than cytochrome B in phylogenetic reconstruction of the Mustelidae (Mammalia:Carnivora)[J].Systematic Biology,2003,52 (5):571-593.
[9] KOEPFLI K P,JENKS S M,EIZIRIK E,ZAHIRPOUR T,et al.Molecular systematics of the Hyaenidae:relationships of a relictual lineage resolved by a molecular supermatrix[J].Molecular Phylogenetics and Evolution,2006,38 (3):603-620.
[10] 荣敏,王雷,吴琼,等.水貂生长激素受体cDNA 克隆与序列分析[J].特产研究,2014,36(1):1-4.
[11] 荣敏,徐佳萍,涂剑锋,等.水貂GHR基因的DNA池测序分析[J].黑龙江畜牧兽医,2017,(10上):240-241,299.
[12] YU Y M,DOMENE H M,SZTEIN J,et al.Developmental changes and differential regulation by testosterone and estradiol of growth hormone receptor expression in the rabbit[J].Eur J Endocrinol,1996,135(5):583-590.
[13] 黄治国,谢庄.绵羊肝脏生长激素受体基因表达的发育性变化研究[J].安徽农业科学,1996,37(13):5 894-5 896.
[14] 易少奎,高泽霞,赵鸿昊,等.团头鲂生长相关基因在不同发育时期生长轴组织的表达分析[J].水生生物学报,2015,39(1):101-109.
[15] 贾斌.新疆绵羊微卫星遗传分析及羊毛生长的神经内分泌调控[D].南京:南京农业大学,2003.
[16] SCHWARTZBAUER G,CHENG H,MENON R K,et al.Expression of novel growth hormone receptor(GHR)transcripts in human heart tissue[J].Growth hormone&IGF Research,1998,8(4):328.
[17] LU C,SCHWARTZBAUER G,SPERLING M A,et al.Demonstration of direct effects of growth hormone on neonatalcardiomyocytes[J].Journal of Biological Chemistry,2001,276(25):22 892-22 900.
[18] 王晓舜,侯大庆.生长激素对脏器功能的影响概述[J].齐鲁药事,2005,(6):362-363.
[16] 石子刚.生长轴激素在猪脾脏中表达及其发育性变化[D].南京:南京农业大学,2002.
[20] 田雨光,王玉珏,岳敏.西藏小型猪不同组织中GHR基因表达的发育性变化[J].黑龙江畜牧兽医,2017(6):104-107,295.
[21] 熊力,陈虹,潘阳阳等.生长激素受体(GHR)在牦牛不同组织中的表达分析[J].农业生物技术学报,2015,23(9):1 234-1 239.