摘要
目的探究祖师麻片对牛Ⅱ型胶原诱发大鼠关节炎的药效学作用。方法采用牛Ⅱ型胶原诱导的关节炎(collagen-induced anhritis,CIA)动物模型,来观察祖师麻片对CIA模型大鼠的关节炎指数(arthritis index,AI),血清中环氧化酶-2(cyclooxygenase-2,COX-2)、结缔组织生长因子(connective tissue growth factor,CTGF)、风湿因子(rheumatoid factor,RF)、免疫球蛋白G(immunoglobulin G,Ig G)、白介素-6(interleukin-6,IL-6)、前列腺素-2(prostaglandin E-2,PGE-2)、肿瘤坏死因子-α(tumor necrosis factor-a,TNF-α)的含量,膝关节番红O-固绿染色,镜下观察关节软骨的一般形态,并对膝关节软骨进行Mankin’ s评分。结果与模型组比较,祖师麻片剂量组大鼠AI评分显著降低(P <0.01);血清中CTGF、Ig G、RF、COX-2、IL-6、PGE-2、TNF-α含量显著降低(P <0.05或P <0.01);软骨细胞数量稍有减少,染色丢失较少,潮线基本完整,Mankin’ s评分评分显著降低(P <0.01)。结论祖师麻片对Ⅱ型胶原诱导的类风湿关节炎有显著改善作用,其机制可能与抗炎、免疫调节作用有关。
Objective To explore the pharmacodynamic effect of Zushima Tablet on bovine type II collagen induced arthritis in rats. Methods An animal model of CIA was used to observe the AI and the serum, COX-2 CTGF, RF, IgG, IL-6, PGE-2 and TNF-α. Knee red O-solid green staining of the knee joint, the general morphology of articular cartilage was observed under the microscope, and the Mankin's score was evaluated for the knee joint cartilage. Results Compared with the model group, the AI scores of the ancestral numb tablet group were significantly lower(P < 0.01). The levels of CTGF, IgG, RF, COX-2, IL-6, PGE-2 and TNF-α in serum were significantly lower(P < 0.05 or P < 0.01). The number of chondrocytes was slightly reduced. The stain loss was less and the tidal line was almost complete. The Mankin's score was significantly lower(P < 0.01). Conclusion Zushima Tablet can significantly improve type II collagen-induced rheumatoid arthritis, which mechanism may be related to anti-inflammatory and immunoregulatory effects.
引文
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