云南省师宗县牛羊蓝舌病病毒感染情况及活动规律监测
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摘要
目的研究蓝舌病病毒近几年在云南省师宗县的感染情况及活动规律。方法 2014-2016年连续3年在师宗县设置监控点,每年选择蓝舌病抗体阴性的10头牛和5只羊作为监控动物,5-10月每周采血1次,11月、12月,每月采血1次,每次每头动物采集常规全血、肝素抗凝血各1份,用于蓝舌病抗体检测和病毒分离。结果 2014-2016年均有动物感染蓝舌病病毒,平均感染率为35.56%,感染时间均在6-10月之间,这一结果表明蓝舌病病毒感染动物主要集中在每年的6-10月,这一时期应加强蓝舌病的防控。在蓝舌病监测中,45只监控动物中的16只感染了蓝舌病病毒,并从7只感染动物中分离出9株蓝舌病病毒,分别为BTV-2、-4、-9、-12、-16型,其中BTV-16型4株,为优势血清型。结论病毒分离和鉴定结果表明,师宗县长期存在多种血清型蓝舌病病毒。
We studied the infection and dynamic patterns of bluetongue virus in Shizong County,Yunnan Province in recent years.Ten healthy cows and 5 healthy goats which were not infected with bluetongue virus were selected to monitor bluetongue in Shizong County from 2014 to 2016 for three consecutive years.In each year,the blood of the selected animals was collected once a week from May to October and once a month in November and December.We used the serum samples and the heparin anticoagulant blood samples for bluetongue antibody detection and virus isolation respectively.Infection rate of bluetongue virus was 35.56%from 2014 to 2016 and the positive seroconverted time of the antibody was June to October.These results indicated that the animals infected with bluetongue virus were mainly focused on June to October of every year and the prevention and control of bluetongue should be strengthened in this period.The 16 of 45 animals have been infected with bluetongue virus,and nine strains BTV have been isolated from seven animals in this Bluetongue monitoring.The nine BTV strains belong to serotypes BTV-2,-4,-9,-12,and-16 in which four strains belong to serotypes BTV-16,suggested that BTV-16 were the prevalent serotype in Shizong County.The results of virus isolation and identification showed that multiple serotype bluetongue virus were prevalent in Shizong County,and exsited in long-term.
We studied the infection and dynamic patterns of bluetongue virus in Shizong County,Yunnan Province in recent years.Ten healthy cows and 5 healthy goats which were not infected with bluetongue virus were selected to monitor bluetongue in Shizong County from 2014 to 2016 for three consecutive years.In each year,the blood of the selected animals was collected once a week from May to October and once a month in November and December.We used the serum samples and the heparin anticoagulant blood samples for bluetongue antibody detection and virus isolation respectively.Infection rate of bluetongue virus was 35.56%from 2014 to 2016 and the positive seroconverted time of the antibody was June to October.These results indicated that the animals infected with bluetongue virus were mainly focused on June to October of every year and the prevention and control of bluetongue should be strengthened in this period.The 16 of 45 animals have been infected with bluetongue virus,and nine strains BTV have been isolated from seven animals in this Bluetongue monitoring.The nine BTV strains belong to serotypes BTV-2,-4,-9,-12,and-16 in which four strains belong to serotypes BTV-16,suggested that BTV-16 were the prevalent serotype in Shizong County.The results of virus isolation and identification showed that multiple serotype bluetongue virus were prevalent in Shizong County,and exsited in long-term.
引文
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[16]Gard GP,Kirkland PD.Bluetongue virology and serology[J].Aus Standard Diagnost Techniq Anim Dis,1993.
[17]肖雷,朱建波,杨仕标,等.云南省边境地区2008-2009年度蓝舌病血清学调查[J].上海畜牧兽医通讯,2010,(6):22-23.DOI:10.3969/j.issn.1000-7725.2010.06.010
[18]李华春,张开礼,李志华.蓝舌病传播媒介云南库蠓的调查[J].云南畜牧兽医,1991(3):86-87.
[19]Rogers DJ,Randolph SE.Studying the global distribution of infectious diseases using GIS and RS[J].Nat Rev Microbiol,2003,1(3):231-237.DOI:10.1038/nrmicro776
[20]Yang H,Xiao L,Wang J,et al.Phylogenetic characterization genome segment 2of bluetongue virus strains belonging to serotypes 5,7and 24isolated for the first time in China during2012to 2014[J].Transbound Emerg Dis,2017,64(4):1317-1321.DOI:10.1111/tbed.12479
[21]苗海生,李乐,朱建波,等.冷藏蓝舌病样品的Real-time PCR检测和病毒颗粒稳定性研究[J].中国畜牧兽医,2013,40(6):41-44.DOI:10.3969/j.issn.1671-7236.2013.06.008
[2]Shoorijeh SJ,Ramin AG,Maclachlan NJ,et al.High seroprevalence of bluetongue virus infection in sheep flocks in West Azerbaijan,Iran[J].Comparat Immunol Microbiol Infect Dis,2010,33(3):243-247.DOI:10.1016/j.cimid.2008.10.008
[3]苗海生,李乐,朱建波,等.蓝舌病病毒阻断ELISA抗体检测方法的建立[J].中国预防兽医学报,2014,36(2):111-115.DOI:10.3969/j.issn.1008-0589.2014.02.07
[4]林汉亮,冉多良,王文.蓝舌病研究进展[J].新疆畜牧业,2008,(1):4-7.DOI:10.16795/j.cnki.xjxmy.2008.01.002
[5]Qin S,Yang H,Zhang Y,et al.Full genome sequence of the first bluetongue virus serotype 21(BTV-21)isolated from China:evidence for genetic reassortment between BTV-21 and bluetongue virus serotype 16(BTV-16)[J].Arch Virol,2018:1-4.DOI:10.1007/s00705-018-3718-9
[6]张胜男,常建华,韩明浩,等.蓝舌病的研究进展[J].黑龙江畜牧兽医,2015(19):57-60.DOI:10.13881/j.cnki.hljxmsy.2015.1753
[7]刘景利,孙建宏,管雪婷,等.蓝舌病的研究进展[J].畜牧兽医科技信息,2005(9):19-21.DOI:10.3969/j.issn.1671-6027.2005.09.007
[8]Cook G.Effect of global warming on the distribution of parasitic and other infectious diseases:a review[J].J R Soc Med,1992,85(11):688-691.
[9]Martens P,Moser SC.Health impacts of climate change[J].Science,2001,292:1065-1066
[10]Patz JA,Campbell-Lendrum D,Holloway T,et al.Impact of regional climate change on human health[J].Nature,2005,438:310-317.DOI:10.1038/nature04188
[11]肖雷,孟锦昕,李楠,等.蓝舌病病毒BTV-1型的分离鉴定[J].上海畜牧兽医通讯,2014(4):5-7.DOI:10.3969/j.issn.1000-7725.2014.04.002
[12]张念祖,张开礼,李志华,等.绵羊蓝舌病的调查研究报告[J].云南畜牧兽医,1989(4):3-13.
[13]肖雷,孟锦昕,李楠,等.云南省师宗县蓝舌病病毒的分离及鉴定[J].中国动物传染病学报,2014,22(4):1-6.
[14]Mertens PP,Burroughs JN,Walton A,et al.Enhanced infectivity of modified bluetongue virus particles for two insect cell lines and for two Culicoides vector species[J].Virology,1996,217:582-593.DOI:10.1006/viro.1996.0153
[15]苗海生,李乐,廖德芳,等.蓝舌病多克隆抗体C-ELISA检测方法的建立和比较研究[J].云南农业大学学报(自然科学),2014,29(1):58-62.DOI:10.3969/j.issn.1004-390X(n).2014.01.012
[16]Gard GP,Kirkland PD.Bluetongue virology and serology[J].Aus Standard Diagnost Techniq Anim Dis,1993.
[17]肖雷,朱建波,杨仕标,等.云南省边境地区2008-2009年度蓝舌病血清学调查[J].上海畜牧兽医通讯,2010,(6):22-23.DOI:10.3969/j.issn.1000-7725.2010.06.010
[18]李华春,张开礼,李志华.蓝舌病传播媒介云南库蠓的调查[J].云南畜牧兽医,1991(3):86-87.
[19]Rogers DJ,Randolph SE.Studying the global distribution of infectious diseases using GIS and RS[J].Nat Rev Microbiol,2003,1(3):231-237.DOI:10.1038/nrmicro776
[20]Yang H,Xiao L,Wang J,et al.Phylogenetic characterization genome segment 2of bluetongue virus strains belonging to serotypes 5,7and 24isolated for the first time in China during2012to 2014[J].Transbound Emerg Dis,2017,64(4):1317-1321.DOI:10.1111/tbed.12479
[21]苗海生,李乐,朱建波,等.冷藏蓝舌病样品的Real-time PCR检测和病毒颗粒稳定性研究[J].中国畜牧兽医,2013,40(6):41-44.DOI:10.3969/j.issn.1671-7236.2013.06.008