尿中硫氰酸盐的酶标仪快速测定法
|
摘要
目的建立尿中硫氰酸盐的酶标仪快速测定法。方法尿样在微酸条件下与氯胺T、吡啶反应,其反应产物与巴比妥酸反应显红色,采用酶标仪测定,并对结果进行肌酐校正。结果硫氰酸盐在40～800μg/L范围内该方法线性关系良好(r=0.999 8),检出限为1.1μg/L,平均回收率为88.9%～101.2%,RSD为5.8%～7.2%。结论该方法简便、快速、灵敏度高,适用于职业人员尿中硫氰酸盐的测定。
Objective To establish a method for rapid determination of thiocyanate in urine samples of workers with the microplate reader. Methods The urine sample reacted with chloramines T and pyridine at weak acid condition, the product from which reacted with barbituric acid and appeared red, then was tested with microplate reader, and the results were corrected with creatinine. Results The linear range was 40-800 μg/L thiocyanate and the correlation coefficient was 0.999 8.The means of recovery rate were 88.9%-101.2% and RSDs were 5.8%-7.2%(n =5).The detection limit was 1.1 μg/L.Conclusion This method is simple, rapid, accurate and is applicable to the determination of thiocyanate in the urine of population with occupational exposure.
Objective To establish a method for rapid determination of thiocyanate in urine samples of workers with the microplate reader. Methods The urine sample reacted with chloramines T and pyridine at weak acid condition, the product from which reacted with barbituric acid and appeared red, then was tested with microplate reader, and the results were corrected with creatinine. Results The linear range was 40-800 μg/L thiocyanate and the correlation coefficient was 0.999 8.The means of recovery rate were 88.9%-101.2% and RSDs were 5.8%-7.2%(n =5).The detection limit was 1.1 μg/L.Conclusion This method is simple, rapid, accurate and is applicable to the determination of thiocyanate in the urine of population with occupational exposure.
引文
[1]张红宇.深圳市健康人群尿中硫氰酸盐生物限值得探讨[J].职业与健康,2009,4(25):380-381.
[2]宋振威,郭平,刘文卫.无锡地区健康人群尿硫氰酸盐正常参考值调查[J].职业卫生与应急救援,2017,35(4):46-47.
[3]张水锋,林珍珍,陈小珍,等.基于离子色谱/电导检测的全脂奶粉中硫氰酸盐污染物的测定及前处理方法的研究[J].分析测试学报,2012,31(1):85-89.
[4]龚敏莉,李波,郝玲妹.丙烯腈从业人员及宁波市居民尿硫氰酸盐水平调查[J].中国卫生检验杂志,2016,26(7):1038-1042.
[5]周元陵,金复生,朱瑞娟,等.尿硫氰酸盐正常参考值及在丙烯腈作业工人健康监护中的应用[J].工业卫生与职业病,2005,31(4):232-236.
[6]王苏华,陆荣柱,邢光伟,等.镇江市健康人群尿硫氰酸盐参考值调查[J].江苏大学学报,2007,17(5):448-450.
[7]陈卫,何彩,陈湘莹.深圳市305名健康成人尿中硫氰酸盐正常参考值的探讨[J].中国工业医学杂志,1996,9(6):359-360.
[2]宋振威,郭平,刘文卫.无锡地区健康人群尿硫氰酸盐正常参考值调查[J].职业卫生与应急救援,2017,35(4):46-47.
[3]张水锋,林珍珍,陈小珍,等.基于离子色谱/电导检测的全脂奶粉中硫氰酸盐污染物的测定及前处理方法的研究[J].分析测试学报,2012,31(1):85-89.
[4]龚敏莉,李波,郝玲妹.丙烯腈从业人员及宁波市居民尿硫氰酸盐水平调查[J].中国卫生检验杂志,2016,26(7):1038-1042.
[5]周元陵,金复生,朱瑞娟,等.尿硫氰酸盐正常参考值及在丙烯腈作业工人健康监护中的应用[J].工业卫生与职业病,2005,31(4):232-236.
[6]王苏华,陆荣柱,邢光伟,等.镇江市健康人群尿硫氰酸盐参考值调查[J].江苏大学学报,2007,17(5):448-450.
[7]陈卫,何彩,陈湘莹.深圳市305名健康成人尿中硫氰酸盐正常参考值的探讨[J].中国工业医学杂志,1996,9(6):359-360.