HPLC法测定芳香化湿口服液中的橙皮苷含量
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摘要
目的建立高效液相色谱法(HPLC)测定医疗机构制剂芳香化湿口服液的含量控制标准,以控制该制剂的质量。方法采用Diamonsil TM~C_18色谱柱;乙腈-0.12%磷酸溶液(20∶80)为流动相;流速为1.0 m L/min;检测波长为284 nm。结果此色谱条件下,可以完全分离橙皮苷。橙皮苷回归方程为Y=1751.1X+116488,相关系数r=0.9999。橙皮苷进样量在122～976 ng范围内线性关系良好(r=0.9999),平均加样回收率为99.99%,RSD=0.88%。结论 HPLC法操作简便、专属性强、重现性好,可有效控制芳香化湿口服液的质量。
Objective To establish the content control standard of aromatic Huashi oral liquid in medical institutions by high performance liquid chromatography(HPLC), in order to control the quality of the preparation. Methods Diamonsil for the determination TM~C_18 column was used, acetonitrile-0.12% phosphoric acid solution(20 ∶80) was used as mobile phase,the flow rate was 1.0 mL/min, and the detection wavelength was 284 nm. Results Under this chromatographic condition,hesperidin could be completely separated. The regression equation of hesperidin was Y =1751.1 X +116488, and the correlation coefficient was 0.9999. The linear relationship between the amount of hesperidin in the range of 122-976 ng was good(r =0.9999). The average recovery of hesperidin was 99.99% and RSD =0.88%. Conclusion HPLC is simple,specific and reproducible, which can effectively control the quality of aromatic Huashi oral liquid.
Objective To establish the content control standard of aromatic Huashi oral liquid in medical institutions by high performance liquid chromatography(HPLC), in order to control the quality of the preparation. Methods Diamonsil for the determination TM~C_18 column was used, acetonitrile-0.12% phosphoric acid solution(20 ∶80) was used as mobile phase,the flow rate was 1.0 mL/min, and the detection wavelength was 284 nm. Results Under this chromatographic condition,hesperidin could be completely separated. The regression equation of hesperidin was Y =1751.1 X +116488, and the correlation coefficient was 0.9999. The linear relationship between the amount of hesperidin in the range of 122-976 ng was good(r =0.9999). The average recovery of hesperidin was 99.99% and RSD =0.88%. Conclusion HPLC is simple,specific and reproducible, which can effectively control the quality of aromatic Huashi oral liquid.
引文
[1]吴愫青,叶莹,张俊.HPLC测定不同产地陈皮橙皮苷的含量[J].中国现代中药,2008,10(8):20-22.
[2]赵陆华.高效液相色谱法分析中药成分手册[M].北京:中国医药科技出版社,1994:129-130.
[3]张好科,付兆媛.西藏不同产区长梗秦艽中龙胆苦苷和马钱苷酸含量研究[J].甘肃中医药大学学报,2016,33(5):24-27.
[4]王帆,孙岩,杨玉华.无糖型黄疸茵陈颗粒的质量标准研究[J].西部中医药,2015,28(6):32-33.
[5]骆勇,苌玲.高效液相色谱法测定参苏口服液中橙皮苷的含量[J].贵阳医学院学报,2012,37(1):71-73.
[6]国家药典委员会.中华人民共和国药典(一部)[M].2015年版.北京:中国医药科技出版社,2015:639.
[7]禤雪梅.HPLC法测定不同产地药材陈皮中橙皮苷的含量[J].北方药学,2015,12(2):9-10.
[8]龙永诚,黎霜,韦忠孙,等.TLC法鉴别复方罗汉果止咳颗粒中的枇杷叶[J].西部中医药,2016,29(3):33-34.
[2]赵陆华.高效液相色谱法分析中药成分手册[M].北京:中国医药科技出版社,1994:129-130.
[3]张好科,付兆媛.西藏不同产区长梗秦艽中龙胆苦苷和马钱苷酸含量研究[J].甘肃中医药大学学报,2016,33(5):24-27.
[4]王帆,孙岩,杨玉华.无糖型黄疸茵陈颗粒的质量标准研究[J].西部中医药,2015,28(6):32-33.
[5]骆勇,苌玲.高效液相色谱法测定参苏口服液中橙皮苷的含量[J].贵阳医学院学报,2012,37(1):71-73.
[6]国家药典委员会.中华人民共和国药典(一部)[M].2015年版.北京:中国医药科技出版社,2015:639.
[7]禤雪梅.HPLC法测定不同产地药材陈皮中橙皮苷的含量[J].北方药学,2015,12(2):9-10.
[8]龙永诚,黎霜,韦忠孙,等.TLC法鉴别复方罗汉果止咳颗粒中的枇杷叶[J].西部中医药,2016,29(3):33-34.