拉萨地区牦牛源黏质沙雷氏菌的分离鉴定及药敏检测
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摘要
为研究拉萨地区牦牛乳中黏质沙雷氏菌的分离鉴定及耐药性情况,试验采用16S rDNA引物对分离株进行PCR鉴定及革兰氏染色和生化鉴定,采用包括头孢菌素类、大环内酯类在内的25种常用抗菌药物进行药敏试验,并人工感染小鼠检测分离菌株的致病性。结果表明:分离菌株在培养基上培养呈现白色,而标准菌株呈现红色;革兰氏染色和生化鉴定结果与黏质沙雷氏菌的标准菌株相同;PCR鉴定结果为黏质沙雷氏菌;对头孢哌酮、环丙沙星等8种抗菌药物敏感,对头孢拉定、万古霉素等15种抗菌药物具有一定的耐药性;致病性不强。说明分离菌株已对多种抗菌药物耐药,这不仅反映了越来越多的公共卫生问题,也为牦牛的疫病防控敲响警钟。
引文
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[16] 封家旺,金红岩,李封赛,等.拉萨市林周县牦牛乳样理化指标及乳房炎病原菌的分离鉴定[J].动物医学进展,2017,38(9):125-127.
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[20] 陈焕,李娟.2012年—2014年粘质沙雷菌的临床分布及耐药性分析[J].中国卫生检验杂志,2016,26(12):1799-1801.
[2] LAKHANI N A,NARSINGHANI U,KUMAR R.Necrotizing fasciitis of the abdominal wall caused by Serratia marcescens[J].Infect Dis Rep,2015,7(2):5774.
[3] SAIDENBERG A B S,TEIXEIRA R H F,ASTOLFI-FERREIRA C S,et al.Serratia marcescens infection in a swallow-tailed hummingbird[J].J Wildl Dis, 2007,43(1):107-110.
[4] MATILLA M A, UDAONDO Z, KRELL T,et al.Genome sequence of Serratia marcescens MSU97, a plant-associated bacterium that makes multiple antibiotics[J].Genome Announc,2017,5(9): e01752-16.
[5] SUNG M J, CHANG C H, YOON Y K, et al.Clinical aspects of an outbreak of Serratia marcescens infections in neonates[J].Korean J Pediatr,2006,49(5):500-506.
[6] KIM S B, JEON Y D, KIM J H, et al.Risk factors for mortality in patients with Serratia marcescens bacteremia[J].Yonsei Med J,2015,56(2):348-354.
[7] BURRITT N L, FOSS N J, NEENO-ECKWALL E C, et al.Sepsis and hemocyte loss in honey bees (apis mellifera) infected with Serratia marcescens strain sicaria[J]. PLoS One, 2016,11(12):e0167752.
[8] TU S, QIU X H, LI C, et al.Expression and characterization of the chitinases from Serratia marcescens GEI strain for the control of varroa destructor, a honey bee parasite[J]. J Invertebr Pathol, 2010,104(2):75-82.
[9] LI B,YU R R,LIU B P,et al. Characterization and comparison of Serratia marcescens isolated from edible cactus and from silkworm for virulence potential and chitosan susceptibility[J]. Braz J Microbiol,2011,42(1):96-104.
[10] ISHII K,ADACHI T,HARA T,et al.Identification of a Serratia marcescens virulence factor that promotes hemolymph bleeding in the silkworm, bombyx mori[J].J Invertebr Pathol,2014,117:61-67.
[11] 黄淑坚,王朝尚.鳄鱼致病性粘质沙雷氏菌感染[J].中国兽医杂志,1998,24(10):53.
[12] GRIMONT P A,GRIMONT F.The genus Serratia[J]. Annu Rev Microbiol,1978,32(1):221.
[13] LEE S H,PARK S J, KWAK D, et al.Sudden death of an Indian peafowl (Pavo cristatus) at a zoo due to non-pigmented Serratia marcescens infection[J]. J Vet Med Sci, 2017,79(12):2048-2051.
[14] BAILEY T A,SILVANOSE C,MANVELL R,et al.Medical dilemmas associated with rehabilitating confiscated houbara bustards (chlamydotis undulata macqueenii) after avian pox and paramyxovirus type 1 infection[J].J Wildl Dis,2002,38(3):518-532.
[15] WORK T M,RAMEYER R A.Mass stranding of wedge-tailed shearwater chicks in Hawaii[J].J Wildl Dis,1999,35(3):487-495.
[16] 封家旺,金红岩,李封赛,等.拉萨市林周县牦牛乳样理化指标及乳房炎病原菌的分离鉴定[J].动物医学进展,2017,38(9):125-127.
[17] 于莉,罗章,孟云,等.西藏地区牦牛乳理化和微生物指标的检测分析[J].中国乳品工业,2006,34(9):8-11.
[18] LEWIS S M,CORPE W A.Prodigiosin-producing bacteria from marine sources[J].Appl Microbiol, 1964,12:13-17.
[19] 潘跃,温珊珊,赵丽丽,等.水貂源致病性黏质沙雷氏菌的分离鉴定与遗传进化分析[J].中国畜牧兽医,2017,44(10):3077-3083.
[20] 陈焕,李娟.2012年—2014年粘质沙雷菌的临床分布及耐药性分析[J].中国卫生检验杂志,2016,26(12):1799-1801.