香菇F2代群体农艺性状的SSR关联分析
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摘要
以香菇(Lentinula edodes)"沪香F2"为亲本,构建F2代群体,测定85个F2代菌株在PDA平板上的菌丝生长速度、菌包中的菌丝生长速度、原基形成时间、出菇周期、菌盖直径、菌柄直径、菌柄长度、单包产量、单包菇数等农艺性状,利用筛选获得的32对具多态性的SSR引物对F2代群体进行基因型检测,共扩增出93个多态性条带,将观测的9个农艺性状与这93个SSR标记进行相关性分析。结果显示,有26个标记能够与这些性状发生关联,关联事件的总数为30;其中有8个标记表现为极显著关联(P<0.01),2个标记与菌柄长度极显著关联,6个标记与单包菇数极显著关联。本研究首次探索了香菇F2代群体在表型上的分化以及与分子标记之间的关联性,为分子标记辅助育种在香菇中的应用提供参考。
Lentinula edodes strain ' Huxiang F2 ' was used as the parent to construct a F2 generation population. The mycelial growth rates of 85 F2 strains on PDA and in the bags were determined. Seven other agronomic traits including primordium formation time, fruiting cycle, pileus diameter, stipe diameter, stipe length, fruit body yield per bag and fruit body number per bag were obtained by two times of cultivation.Thirty-two pairs of polymorphic SSR primers obtained after screening were used to dertermine the genotypes of the F2 population and 93 polymorphic bands were obtained. The 9 agronomic traits observed were correlated with these SSR markers. The results showed that there were 26 markers associated with these traits, the total number of associated events was 30, of which 8 markers showed a significant association(P<0.01). Two markers were significantly associated with the stipe length and six markers were significantly associated(P<0.01) with the fruit body number per bag. This study explored phenotypic differentiations of the F2 generation of L. edodes and their association with molecular markers for the first time, which provided a reference for the application of molecular marker-assisted breeding of L. edodes.
Lentinula edodes strain ' Huxiang F2 ' was used as the parent to construct a F2 generation population. The mycelial growth rates of 85 F2 strains on PDA and in the bags were determined. Seven other agronomic traits including primordium formation time, fruiting cycle, pileus diameter, stipe diameter, stipe length, fruit body yield per bag and fruit body number per bag were obtained by two times of cultivation.Thirty-two pairs of polymorphic SSR primers obtained after screening were used to dertermine the genotypes of the F2 population and 93 polymorphic bands were obtained. The 9 agronomic traits observed were correlated with these SSR markers. The results showed that there were 26 markers associated with these traits, the total number of associated events was 30, of which 8 markers showed a significant association(P<0.01). Two markers were significantly associated with the stipe length and six markers were significantly associated(P<0.01) with the fruit body number per bag. This study explored phenotypic differentiations of the F2 generation of L. edodes and their association with molecular markers for the first time, which provided a reference for the application of molecular marker-assisted breeding of L. edodes.
引文
[1]中国食用菌协会关于印发全国食用菌2016年度产量、产值和出口统计调查结果的函[EB/OL].[2017-10-24].http://www.cefa.org.cn/2017/10/24/10250.html.
[2]肖扬,龚文兵,边银丙.关联分析及其在真菌遗传学研究中的应用[J].菌物学报,2016,35(7):782-790.
[3] LI C, GONG WB, ZHANG L, et al. Association mapping reveals genetic loci associated with important agronomic traits in Lentinula edodes, shiitake mushroom[J]. Frontiers in Microbiology, 2017, 8:237.
[4] GONG WB, LI L, ZHOU Y, et al. Genetic dissection of fruiting body-related traits using quantitative trait loci mapping in Lentinula edodes[J]. Applied Microbiology Biotechnology, 2016, 100(12):5437-5452.
[5]刘高磊.中国香菇重要性状的全基因组关联分析[D].武汉:华中农业大学,2017.
[6]徐锐.野生香菇数量性状与SSR分子标记的关联分析[D].武汉:华中农业大学,2013.
[7]郑光凤.香菇申香16自交S1代若干数量性状的遗传分化研究[D].南京:南京农业大学,2010.
[8]徐珍,尚晓冬,谭琦.多孢自交选育工厂化栽培的金针菇菌种[J].食用菌学报,2012,19(4):11-13.
[9]宋春艳,杨晓春,张丹,等.香菇L808菌株自交子代在变温培养中菌丝生长速度的分化[J].食用菌学报,2015,22(3):17-20.
[10]张旬旬,苏蓉,尚晓冬,等.工厂化生产金针菇菌株'青禾'F2代性状分化的研究[J].上海农业学报,2014,30(5):28-31.
[11]苏蓉.金针菇自交子代群体生物学性状的研究[D].南京:南京农业大学,2008.
[12]张美彦,尚晓冬,谭琦,等.香菇135菌株特异SCAR标记的分布和遗传特性[J].食用菌学报,2008, 15(4):1-5.
[13]秦莲花,张红,陈明杰.微卫星(TATG)n基序在香菇菌种中的验证[J].微生物学报,2004, 44(4):474-478.
[14]张丹,巫萍,章炉军,等.基于香菇全基因组序列开发的部分SSR标记多态性分析与品种鉴定初探[J].食用菌学报,2012, 19(4):1-10.
[15] NELSON JC, ANDREESCU C, BRESEGHELLO F, et al. Quantitative trait locus analysis of wheat quality traits[J]. Euphytica, 2006, 149(1-2):145-159.
[16]王智兰.小麦蛋白磷酸酶Tapp2A a/c的功能标记开发、作图和关联分析[D].太原:山西大学,2011.
[17] YAN JB, KANDIANIS CB, HAIJIES CE, et al. Rare genetic variation at Zea mays crtRBl increasesβ-carotene in maize grain[J]. Nature Genetics, 2010, 42(4):322-329.
[18]龚文兵,刘伟,卢颖颖,等.基于F2群体的香菇遗传图谱构建及其在QTL定位中的应用[J].菌物学报,2014,33(2):297-311.
[19]金群力,沈颖越,蔡为明,等.金针菇基因组SSR位点分析及标记开发[J].食用菌学报,2016,23(2):12-19.
[20]王新新,李丹,宋冰,等.双孢蘑菇种质SSR分子身份证的构建[J].食用菌学报,2016,23(2):6-11.
[21] XIAO Y, LIU W, DAI YH, et al. Using SSR markers to evaluate the genetic diversity of Lentinula edodes'natural germplasm in China[J]. World Journal of Microbiology and Biotechnology,2010,26(3):527-536.
[22] XIAO Y, CHENG X, LIU J,et al. Population genomic analysis uncovers environmental stress-driven selection and adaptation of Lentinula edodes population in China[J]. Scientific Report, 2016, 6:36789.
[23] XIANG XJ, LI C, LI L, et al. Genetic diversity and population structure of Chinese Lentinula edodes revealed by InDel and SSR markers[J]. Mycological Progress, 2016,15(4):37.
[24]张丹,宋春艳,章炉军,等.基于全基因组序列的香菇商业菌种SSR遗传多样性分析及多位点指纹图谱构建的研究[J].食用菌学报,2014,21(2):1-13.
[25]文自翔,赵团结,郑永战,等.中国栽培和野生大豆农艺品质性状与SSR标记的关联分析I群体结构及关联分析标记[J].作物学报,2008, 34(7):1169-1178.
[26]郝德荣.大豆产量相关性状QTL的关联分析及候选基因GmGA30x单倍型鉴定[D].南京:南京农业大学,2011.
[27]徐亮.玉米自交系遗传多样性及产量性状与SSR标记的关联分析[D].扬州:扬州大学,2012.
[28]何静.木薯主栽品种/品系农艺性状与分子标记的关联分析[D].海南:海南大学,2010.
[29]方俐.基于SSR标记的豌豆遗传连锁图谱加密及抗冻基因关联分析[D].北京:中国农业科学院,2016.
[2]肖扬,龚文兵,边银丙.关联分析及其在真菌遗传学研究中的应用[J].菌物学报,2016,35(7):782-790.
[3] LI C, GONG WB, ZHANG L, et al. Association mapping reveals genetic loci associated with important agronomic traits in Lentinula edodes, shiitake mushroom[J]. Frontiers in Microbiology, 2017, 8:237.
[4] GONG WB, LI L, ZHOU Y, et al. Genetic dissection of fruiting body-related traits using quantitative trait loci mapping in Lentinula edodes[J]. Applied Microbiology Biotechnology, 2016, 100(12):5437-5452.
[5]刘高磊.中国香菇重要性状的全基因组关联分析[D].武汉:华中农业大学,2017.
[6]徐锐.野生香菇数量性状与SSR分子标记的关联分析[D].武汉:华中农业大学,2013.
[7]郑光凤.香菇申香16自交S1代若干数量性状的遗传分化研究[D].南京:南京农业大学,2010.
[8]徐珍,尚晓冬,谭琦.多孢自交选育工厂化栽培的金针菇菌种[J].食用菌学报,2012,19(4):11-13.
[9]宋春艳,杨晓春,张丹,等.香菇L808菌株自交子代在变温培养中菌丝生长速度的分化[J].食用菌学报,2015,22(3):17-20.
[10]张旬旬,苏蓉,尚晓冬,等.工厂化生产金针菇菌株'青禾'F2代性状分化的研究[J].上海农业学报,2014,30(5):28-31.
[11]苏蓉.金针菇自交子代群体生物学性状的研究[D].南京:南京农业大学,2008.
[12]张美彦,尚晓冬,谭琦,等.香菇135菌株特异SCAR标记的分布和遗传特性[J].食用菌学报,2008, 15(4):1-5.
[13]秦莲花,张红,陈明杰.微卫星(TATG)n基序在香菇菌种中的验证[J].微生物学报,2004, 44(4):474-478.
[14]张丹,巫萍,章炉军,等.基于香菇全基因组序列开发的部分SSR标记多态性分析与品种鉴定初探[J].食用菌学报,2012, 19(4):1-10.
[15] NELSON JC, ANDREESCU C, BRESEGHELLO F, et al. Quantitative trait locus analysis of wheat quality traits[J]. Euphytica, 2006, 149(1-2):145-159.
[16]王智兰.小麦蛋白磷酸酶Tapp2A a/c的功能标记开发、作图和关联分析[D].太原:山西大学,2011.
[17] YAN JB, KANDIANIS CB, HAIJIES CE, et al. Rare genetic variation at Zea mays crtRBl increasesβ-carotene in maize grain[J]. Nature Genetics, 2010, 42(4):322-329.
[18]龚文兵,刘伟,卢颖颖,等.基于F2群体的香菇遗传图谱构建及其在QTL定位中的应用[J].菌物学报,2014,33(2):297-311.
[19]金群力,沈颖越,蔡为明,等.金针菇基因组SSR位点分析及标记开发[J].食用菌学报,2016,23(2):12-19.
[20]王新新,李丹,宋冰,等.双孢蘑菇种质SSR分子身份证的构建[J].食用菌学报,2016,23(2):6-11.
[21] XIAO Y, LIU W, DAI YH, et al. Using SSR markers to evaluate the genetic diversity of Lentinula edodes'natural germplasm in China[J]. World Journal of Microbiology and Biotechnology,2010,26(3):527-536.
[22] XIAO Y, CHENG X, LIU J,et al. Population genomic analysis uncovers environmental stress-driven selection and adaptation of Lentinula edodes population in China[J]. Scientific Report, 2016, 6:36789.
[23] XIANG XJ, LI C, LI L, et al. Genetic diversity and population structure of Chinese Lentinula edodes revealed by InDel and SSR markers[J]. Mycological Progress, 2016,15(4):37.
[24]张丹,宋春艳,章炉军,等.基于全基因组序列的香菇商业菌种SSR遗传多样性分析及多位点指纹图谱构建的研究[J].食用菌学报,2014,21(2):1-13.
[25]文自翔,赵团结,郑永战,等.中国栽培和野生大豆农艺品质性状与SSR标记的关联分析I群体结构及关联分析标记[J].作物学报,2008, 34(7):1169-1178.
[26]郝德荣.大豆产量相关性状QTL的关联分析及候选基因GmGA30x单倍型鉴定[D].南京:南京农业大学,2011.
[27]徐亮.玉米自交系遗传多样性及产量性状与SSR标记的关联分析[D].扬州:扬州大学,2012.
[28]何静.木薯主栽品种/品系农艺性状与分子标记的关联分析[D].海南:海南大学,2010.
[29]方俐.基于SSR标记的豌豆遗传连锁图谱加密及抗冻基因关联分析[D].北京:中国农业科学院,2016.