月影寿的组织培养与快速繁殖
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摘要
选用月影寿Haworthia Tsukikage幼嫩花茎作为试验材料,研究不同消毒时间和灭菌处理对外植体的影响,结果表明,月影寿花茎的最佳灭菌处理为75%乙醇处理30s,0.1%HgCl2处理5～7min。应用正交试验设计研究基本培养基、植物生长调节剂配比及质量浓度水平变化对花茎愈伤组织诱导、分化和生根的影响,结果表明,最佳愈伤组织诱导培养基为:MS+2.0mg·L-1 6-BA+0.3mg·L-1 NAA+1.0mg·L-1 KT;最佳愈伤组织分化培养基为:1.0mg·L-1 6-BA+0.1mg·L-1 NAA+1.5mg·L-1 KT;最佳生根诱导培养基为1/2MS+0.1mg·L-1 NAA+5g·L-1活性炭,组培生根苗移栽成活率在85%以上。初步建立了月影寿组织培养与快速繁殖技术体系,为规模化生产提供了技术支持。
An experiment was conducted using young scapes of Haworthia Tsukikage to study the disinfection of explants for tissue culture and propagation.The choice treatment was determined to immerse the scape cuts in 75%ethanol for 30 sfollowed by 0.1% HgCl2 for 5-7min.The orthogonal experiment rendered the optimized medium for the callus induction to be MS+6-BA 2.0mg·L-1+NAA 0.3mg·L-1+KT 1.0mg·L-1;that for the callus differentiation,6-BA 1.0mg·L-1+NAA 0.1mg·L-1+KT 1.5mg·L-1;and that for the rooting,1/2MS+NAA 0.1mg·L-1+activated carbon 5g·L-1.The survival rate of the transplanted seedlings was approximately85%.A preliminary method of the tissue culture and rapid propagation for H.Tsukikage was thus established.Further refinement for scaleup operation is in order.
An experiment was conducted using young scapes of Haworthia Tsukikage to study the disinfection of explants for tissue culture and propagation.The choice treatment was determined to immerse the scape cuts in 75%ethanol for 30 sfollowed by 0.1% HgCl2 for 5-7min.The orthogonal experiment rendered the optimized medium for the callus induction to be MS+6-BA 2.0mg·L-1+NAA 0.3mg·L-1+KT 1.0mg·L-1;that for the callus differentiation,6-BA 1.0mg·L-1+NAA 0.1mg·L-1+KT 1.5mg·L-1;and that for the rooting,1/2MS+NAA 0.1mg·L-1+activated carbon 5g·L-1.The survival rate of the transplanted seedlings was approximately85%.A preliminary method of the tissue culture and rapid propagation for H.Tsukikage was thus established.Further refinement for scaleup operation is in order.
引文
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[3]王燕,牟豪杰,吕永平,等.寿锦的离体植株再生及组培产业化增殖[J].植物学报,2017,52(3):331-336.
[4]胡松梅.冰城寿离体培养与植株再生[J].南方农业,2017,11(18):116-117.
[5]郭艳.帝王寿的组织培养与快速繁殖[J].绿色科技,2017,10(19):120-121.
[6]陈正华.木本植物组织培养及其应用[M].北京:高等教育出版社,1986:30-34.
[7]杜荣骞.生物统计学[M].北京:高等教育出版社,1989:419.
[8]孙涛,金蕊,李德森.康平寿的组织培养与快速繁殖[J].植物生理学通讯,2003,39(3):232.
[9]左志宇,李建希,安晓云,等.克里克特寿的组织培养与快速繁殖[J].植物生理学通讯,2007,43(2):311-312.
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[12]王紫珊,王广东,王雁.多肉植物白银寿‘奇迹'的离体培养与快速繁殖[J].基因组学与应用生物学,2014,33(6):1329-1335.