外源硒调控盐胁迫下小白菜种子萌发特性和生理特性研究
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摘要
以"四季小白菜""京新1号""鲁抗快菜"小白菜种子为试材,采用水培法,研究了NaCl和Na_2SeO_3处理对小白菜种子发芽势、发芽率、发芽指数、活力指数、相对盐害率、耐盐指数、苗长以及丙二醛(MDA)含量和超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性的影响,以探讨不同品种小白菜耐盐性差异并揭示硒元素缓解小白菜盐胁迫的作用效果。结果表明:150mmol·L~(-1)和200mmol·L~(-1) NaCl处理中"四季小白菜"种子的相对盐害率为3.33%和30.00%,"京新1号"种子的相对盐害率为-8.22%和27.4%,"鲁抗快菜"种子的相对盐害率为25.34%和56.36%,表明"鲁抗快菜"耐盐性最弱。同时,150mmol·L~(-1) NaCl处理和1mg·L~(-1) Na_2SeO_3+150mmol·L~(-1) NaCl处理中小白菜种子发芽势、发芽率、发芽指数、活力指数和株高均显著低于对照,但1 mg·L~(-1)Na_2SeO_3+150mmol·L~(-1) NaCl处理中小白菜种子发芽势、发芽率、发芽指数、活力指数和株高较150 mmol·L~(-1) NaCl处理分别提高17.37%、5.52%、5.36%、159.87%和143.91%,相对盐害率降低为8.28%,耐盐指数增加到77.07%。此外,与150mmol·L~(-1)NaCl处理相比,1mg·L~(-1) Na_2SeO_3+150mmol·L~(-1) NaCl处理中小白菜幼苗MDA含量显著降低,POD活性和CAT活性均显著升高,故1mg·L~(-1) Na_2SeO_3能够缓解盐胁迫对小白菜幼苗的毒害。
The current study explored salt tolerance difference of different varieties in pakchoi and illustrated effects of exogenous selenium(Na_2SeO_3)on germination feature and physiological property under salt stress in pakchoi in order to reveal function of selenium remitting salt stress.‘Four seasons pakchoi'‘Jinxin one'‘Lukang'were cultivated in manual climatic box with 70% humidity and 25℃.The effects of NaCl and selenium on seed germination potential,germination rate,germination index,vigor index,relative salt-harmed rate,salt-tolerance index,seedling length and MDA content,as well as antioxidant enzymes(SOD,POD and CAT)activities were illustrated.The results showed that seed relative salt-harmed rate was 3.33% and 30.00% for‘Four seasons pakchoi',-8.22% and 27.4%for‘Jinxin one',25.34% and 56.36% for‘Lukang'in 150 mmol·L~(-1) NaCl and 200 mmol·L~(-1)NaCl treatments,respectively.Thus,‘Lukang'had the weakest salt tolerance among selected different varieties in pakchoi.Meanwhile,germination potential,germination rate,germination index,vigor index,salt-tolerance index,and seedling length in 150 mmol·L~(-1) NaCl treatment and 1 mg·L~(-1)Na_2SeO_3+150mmol·L~(-1) NaCl treatments were significant lower than those in control.However,germination potential,germination rate,germination index,vigor index,salt-tolerance index,and seedling length in 1mg·L~(-1) Na_2SeO_3+100mmol·L~(-1) NaCl treatment were increased by 17.37%,5.52%,5.36%,159.87% and 143.91%,respectively,in comparison with those in 150 mmol·L~(-1)NaCl treatment.Relative salt-harmed rate was decreased to 8.28% and salt tolerance index was increased to 77.07%.In addition,MDA content was decreased significantly in 1mg·L~(-1) Na_2SeO_3+150mmol·L~(-1) NaCl treatment while POD and CAT activities was increased.Therefore,1mg·L~(-1)Na_2SeO_3 could relieve salt stress in pakchoi.
The current study explored salt tolerance difference of different varieties in pakchoi and illustrated effects of exogenous selenium(Na_2SeO_3)on germination feature and physiological property under salt stress in pakchoi in order to reveal function of selenium remitting salt stress.‘Four seasons pakchoi'‘Jinxin one'‘Lukang'were cultivated in manual climatic box with 70% humidity and 25℃.The effects of NaCl and selenium on seed germination potential,germination rate,germination index,vigor index,relative salt-harmed rate,salt-tolerance index,seedling length and MDA content,as well as antioxidant enzymes(SOD,POD and CAT)activities were illustrated.The results showed that seed relative salt-harmed rate was 3.33% and 30.00% for‘Four seasons pakchoi',-8.22% and 27.4%for‘Jinxin one',25.34% and 56.36% for‘Lukang'in 150 mmol·L~(-1) NaCl and 200 mmol·L~(-1)NaCl treatments,respectively.Thus,‘Lukang'had the weakest salt tolerance among selected different varieties in pakchoi.Meanwhile,germination potential,germination rate,germination index,vigor index,salt-tolerance index,and seedling length in 150 mmol·L~(-1) NaCl treatment and 1 mg·L~(-1)Na_2SeO_3+150mmol·L~(-1) NaCl treatments were significant lower than those in control.However,germination potential,germination rate,germination index,vigor index,salt-tolerance index,and seedling length in 1mg·L~(-1) Na_2SeO_3+100mmol·L~(-1) NaCl treatment were increased by 17.37%,5.52%,5.36%,159.87% and 143.91%,respectively,in comparison with those in 150 mmol·L~(-1)NaCl treatment.Relative salt-harmed rate was decreased to 8.28% and salt tolerance index was increased to 77.07%.In addition,MDA content was decreased significantly in 1mg·L~(-1) Na_2SeO_3+150mmol·L~(-1) NaCl treatment while POD and CAT activities was increased.Therefore,1mg·L~(-1)Na_2SeO_3 could relieve salt stress in pakchoi.
引文
[1]卢艳敏,苏长青,李会芬.不同盐胁迫对白三叶种子萌发及幼苗生长的影响[J].草业学报,2013,22(4):123-129.
[2]刘自刚,王志江,方圆,等.NaCl胁迫对白菜型冬油菜种子萌发和幼苗生理的影响[J].中国油料作物学报,2017,39(3):351-359.
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[5]李辉,康健,赵耕毛,等.盐胁迫对菊芋干物质和糖分积累分配的影响[J].草业学报,2014,23(2):160-170.
[6]彭斌,许伟,邵荣,等.盐胁迫对不同生境种源盐地碱蓬幼苗生长、光合色素及渗透调节物质的影响[J].海洋湖沼通报,2017(1):63-72.
[7]武俊英,秦丽,杨进,等.盐胁迫对农大甜研6号甜菜幼苗生长和养分运移的研究[J].作物杂志,2017(3):75-80.
[8]孟祥浩,刘义国,张玉梅,等.盐胁迫对不同耐盐类型小麦花后光合特性及产量的影响[J].中国农学通报,2015,31(24):46-52.
[9]罗成科,肖国举,张峰举,等.不同浓度复合盐胁迫对水稻产量和品质的影响[J].干旱区资源与环境,2017,31(1):137-141.
[10]GASECKA M,MLECZEK M,SIWULSKI M,et al.Phenolic composition and antioxidant properties of Pleurotus ostreatus and Pleurotus eryngii enriched with selenium and zinc[J].Eur Food Res Technol,2016,242:723-732.
[11]FENG R W,WEI C Y,TU S X.The roles of selenium in protecting plants against abiotic stresses[J].Environmental and Experimental Botany,2013,87:58-68.
[12]PROIETTIA P,NASINIA L,BUONOA D D,et al.Selenium protects olive(OleaeuropaeaL.)from drought stress[J].Scientia Horticulturae,2013,164:165-171.
[13]YAO X Q,CHU J Z,HE X L,et al.Effects of selenium on agronomical characters of winter wheat exposed to enhance ultraviolet-B[J].Ecotoxicology and Environmental Safety,2013,92:320-326.
[14]PENG L,JIA F,TIAN X P,et al.Alleviation of cadmium stress on root tip of rape seedlings by selenium[J].Acta Scientiae Circumstantiae,2015,35(8):2597-2604.
[15]贾玮,吴隽,屈婵娟,等.硒增强植物抗逆能力及其机理研究进展[J].中国农学通报,2015,31(14):171-176.
[16]张利霞,常青山,侯小改,等.不同钠盐胁迫对夏枯草种子萌发特性的影响[J].草业学报,2015,24(3):177-186.
[17]张志良,瞿伟菁,李小芳.植物生理学实验指导[M].4版.北京:高等教育出版社,2009.
[18]王激清,刘社平,白晓瑛.盐胁迫对不同品种甜菜种子萌发特性的影响[J].江苏农业科学,2015,43(3):96-98.
[19]李志萍,张文辉,崔豫川.NaCl和Na2CO3胁迫对栓皮栎种子萌发及幼苗生长的影响[J].生态学报,2015,35(3):742-751.
[20]林宝刚,张晓峰.氯化钠对甘蓝型油菜种子萌发的胁迫效应[J].浙江农业学报,2010,22(5):624-627.
[21]邱念伟,陈敏,杨洪兵,等.伽蓝菜和碱蓬耐旱耐盐机理的比较研究[J].山东科学,2001,14(1):5-10.
[22]韩广泉,李俊,宋曼曼,等.硒对盐胁迫下加工番茄种子萌发及抗氧化酶系统的影响[J].石河子大学学报(自然科学版),2010,28(4):422-426.
[23]李彦,张银鹏,孙明,等.盐胁迫条件下硒对小白菜抗氧化活性及膜脂过氧化作用的影[J].植物营养与肥料学报,2008,14(4):749-753.
[2]刘自刚,王志江,方圆,等.NaCl胁迫对白菜型冬油菜种子萌发和幼苗生理的影响[J].中国油料作物学报,2017,39(3):351-359.
[3]徐晨,凌风楼,徐克章,等.盐胁迫对不同水稻品种光合特性和生理生化特性的影响[J].中国水稻科学,2013,27(3):280-286.
[4]蔡金桓,都成林,薛立,等.盐胁迫对4种园林植物光合特性的影响[J].西南林业大学学报,2017,37(2):30-34.
[5]李辉,康健,赵耕毛,等.盐胁迫对菊芋干物质和糖分积累分配的影响[J].草业学报,2014,23(2):160-170.
[6]彭斌,许伟,邵荣,等.盐胁迫对不同生境种源盐地碱蓬幼苗生长、光合色素及渗透调节物质的影响[J].海洋湖沼通报,2017(1):63-72.
[7]武俊英,秦丽,杨进,等.盐胁迫对农大甜研6号甜菜幼苗生长和养分运移的研究[J].作物杂志,2017(3):75-80.
[8]孟祥浩,刘义国,张玉梅,等.盐胁迫对不同耐盐类型小麦花后光合特性及产量的影响[J].中国农学通报,2015,31(24):46-52.
[9]罗成科,肖国举,张峰举,等.不同浓度复合盐胁迫对水稻产量和品质的影响[J].干旱区资源与环境,2017,31(1):137-141.
[10]GASECKA M,MLECZEK M,SIWULSKI M,et al.Phenolic composition and antioxidant properties of Pleurotus ostreatus and Pleurotus eryngii enriched with selenium and zinc[J].Eur Food Res Technol,2016,242:723-732.
[11]FENG R W,WEI C Y,TU S X.The roles of selenium in protecting plants against abiotic stresses[J].Environmental and Experimental Botany,2013,87:58-68.
[12]PROIETTIA P,NASINIA L,BUONOA D D,et al.Selenium protects olive(OleaeuropaeaL.)from drought stress[J].Scientia Horticulturae,2013,164:165-171.
[13]YAO X Q,CHU J Z,HE X L,et al.Effects of selenium on agronomical characters of winter wheat exposed to enhance ultraviolet-B[J].Ecotoxicology and Environmental Safety,2013,92:320-326.
[14]PENG L,JIA F,TIAN X P,et al.Alleviation of cadmium stress on root tip of rape seedlings by selenium[J].Acta Scientiae Circumstantiae,2015,35(8):2597-2604.
[15]贾玮,吴隽,屈婵娟,等.硒增强植物抗逆能力及其机理研究进展[J].中国农学通报,2015,31(14):171-176.
[16]张利霞,常青山,侯小改,等.不同钠盐胁迫对夏枯草种子萌发特性的影响[J].草业学报,2015,24(3):177-186.
[17]张志良,瞿伟菁,李小芳.植物生理学实验指导[M].4版.北京:高等教育出版社,2009.
[18]王激清,刘社平,白晓瑛.盐胁迫对不同品种甜菜种子萌发特性的影响[J].江苏农业科学,2015,43(3):96-98.
[19]李志萍,张文辉,崔豫川.NaCl和Na2CO3胁迫对栓皮栎种子萌发及幼苗生长的影响[J].生态学报,2015,35(3):742-751.
[20]林宝刚,张晓峰.氯化钠对甘蓝型油菜种子萌发的胁迫效应[J].浙江农业学报,2010,22(5):624-627.
[21]邱念伟,陈敏,杨洪兵,等.伽蓝菜和碱蓬耐旱耐盐机理的比较研究[J].山东科学,2001,14(1):5-10.
[22]韩广泉,李俊,宋曼曼,等.硒对盐胁迫下加工番茄种子萌发及抗氧化酶系统的影响[J].石河子大学学报(自然科学版),2010,28(4):422-426.
[23]李彦,张银鹏,孙明,等.盐胁迫条件下硒对小白菜抗氧化活性及膜脂过氧化作用的影[J].植物营养与肥料学报,2008,14(4):749-753.