室内冷冻法鉴定小麦抗寒性的研究
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摘要
冬季冻害是制约我国北方小麦生产的重要因素之一,田间鉴定小麦抗寒性方法易受环境影响。本研究以209份抗寒性存在显著差异的小麦种质资源为材料,进行人工冷冻处理,测定不同冷冻温度处理下植株电导率及冷冻成活率,分析相对电导率、冷冻成活率和半致死温度与田间冻害等级的相关性,以建立室内鉴定小麦抗寒性的最适方法,为小麦抗寒性育种提供优良品种资源。结果表明,-14℃处理3 h后的冷冻成活率与冻害等级呈显著负相关,该方法可快速、准确鉴定小麦不同品种抗寒性,同时鉴定出河农05(9)7-11-4-15、冀麦585、乐639、石麦15、保5108、石新828、众麦1号和河农7069等抗寒性较强品种。
Winter freezing seriously threatens the yield stability of common wheat in the north area of China.Test for freezing resistance under the field condition largely depended on the environmental climate.By taking use of artificial freezing,we investigated the relative conductivity and freezing survival rate of the plants under different freezing treatments in 209 wheat varieties,with a purpose of obtaining excellent variety resources for cold resistance breeding of wheat.Furthermore,we analyzed the correlation of relative conductivity,freezing survival rate and lethal temperature with field frost damage grade.The results showed a significant negative correlation between the freezing survival rate and freezing injury level after treatment for 3 h at-14 ℃(correlation coefficient=-0.427).By cold treatment under different temperatures,we found the optimal temperature condition that could be used to identify the cold resistance at seedling stage.As a result,several cold resistance varieties such as Henong 05(9)7-11-4-15,Jimai 585,Le 639,Shimai 15,Bao 5108,Shixin 828,Zhongmai 1 and Henong7069 were obtained.
Winter freezing seriously threatens the yield stability of common wheat in the north area of China.Test for freezing resistance under the field condition largely depended on the environmental climate.By taking use of artificial freezing,we investigated the relative conductivity and freezing survival rate of the plants under different freezing treatments in 209 wheat varieties,with a purpose of obtaining excellent variety resources for cold resistance breeding of wheat.Furthermore,we analyzed the correlation of relative conductivity,freezing survival rate and lethal temperature with field frost damage grade.The results showed a significant negative correlation between the freezing survival rate and freezing injury level after treatment for 3 h at-14 ℃(correlation coefficient=-0.427).By cold treatment under different temperatures,we found the optimal temperature condition that could be used to identify the cold resistance at seedling stage.As a result,several cold resistance varieties such as Henong 05(9)7-11-4-15,Jimai 585,Le 639,Shimai 15,Bao 5108,Shixin 828,Zhongmai 1 and Henong7069 were obtained.
引文
[1]郑大伟,李茂松,霍志国.2008年南方低温冰雪灾害对农业的影响及对策.防灾科技学院学报,2008,10(2):1-4Zheng D W,Li M S,Huo Z G.Effects of 2008 snow disaster in southern China on agriculture and countermeasures.Journal of Institute of Disaster-Prevention Science and Technology,2008,10(2):1-4
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[5]刘艳阳,李俊周,陈磊,崔党群.低温胁迫对小麦叶片细胞膜脂质过氧化产物及相关酶活性的影响.麦类作物学报,2006,26(4):70-73Liu Y Y,Li J Z,Chen L,Cui D Q.Effect of low temperature stress on peroxidation product of membrane lipids and activity of related enzymes in wheat seedling leaves.Journal Triticeae Crops,2006,26(4):70-73
[6]Bohn M,Luthje S.Plasma membrane lipid alterations induced by cold acclimation and abscisic acid treatment of winter wheat seedlings differing in frost resistance.Plant Physiology,2007(164):146-156
[7]朱根海,刘祖祺,朱培仁.应用logistic方程确定植物组织低温半致死温度的研究.南京农业大学学报,1986,9(3):11-16Zhu G H,Liu Z Q,Zhu P R.Application of Logistic equation to determine the semi lethal temperature of plant tissues at low temperature.Journal of Nanjing Agricultural University,1986,9(3):11-16
[8]姜丽娜,张黛静,宋飞,刘佩,樊婷婷,余海波,李春喜.不同品种小麦叶片对拔节期低温的生理响应及抗寒性评价.生态学报,2014,34(15):4251-4261Jiang L N,Zhang D J,Song F,Liu P,Fan T T,Yu H B,Li CX.Evaluation of cold resistance of different wheat varieties based on physiological responses of leaves to low temperature at the jointing stage.Acta Ecologica Sinica,2014,34(15):4251-4261
[9]Yan L,Fu D M,Li C C,Blechl A,Tranquilli G,Bonafede M,Sanchez A,Valarik M,Yasuda S,Dubcovsky J.The wheat and barley vernalization gene Vrn3 is an orthologue of FT.Proceedings of the National Academy of Sciences,2006(103):19581-19586
[10]牟永潮,崔红,于晶,苍晶,曾俨,孟健男.低温胁迫下东农冬麦1号分蘖节SSH文库的构建及文库中3个基因的表达模式.作物学报,2011,37(5):918-923Mu Y C,Cui H,Yu J,Cang J,Zeng Y,Meng J N.Expression analysis of three genes from SSH library constructed using tillering nodes of Dongnongdongmai 1 under low temperature.Acta Agronomica Sinica,2011,37(5):918-923
[11]Badawi M,Danyluk J,Boucho B,Sarhan F.The CBFgene family in hexaploid wheat and its relationship to the phylogenetic complexity of cereal CBFs.Molecular Genetics and Genomics,2007,277(5):533-554
[12]Fowler D B,Limin A E,Wang S Y,Ward R W.Relationship between low-temperature tolerance and vernalization response in wheat and rye.Canadian Journal of Plant Science,1996,76(1):37-42
[13]Mastrangelo A M,Belloni S,Barilli S,Cattivelli L.Low temperature promotes intron retention in two ecor genes of durum wheat.Planta,2005,221(5):705-715
[14]赵玉田,梁博文.冬小麦抗冻性鉴定的方法指标及筛选.中国农业科学,1987,20(6):74-80Z h a o Y T,L i a n g B W.M e t h o d s a n d s c r e e n i n g o f t h e identification of winter wheat frost resistance.Scientia Agriculture Sinica,1987,20(6):74-80
[15]李卓夫,李莹,王晓楠.不同抗寒性冬小麦品种间冷冻后电导率与成活率比较分析.东北农业大学学报,2014,45(6):1-5Li Z F,Li Y,Wang X N.Comparative analysis of electrical conductivity and survival rate on winter wheat varieties with different cold resistances after freezing treatment.Journal of Northeast Agricultural University,2014,45(6):1-5
[16]申宗坦.作物育种学实验.北京:中国农业出版社,1992Shen Z T.Experiment of crop breeding.Beijing:China Agriculture Press,1992
[17]Gusta L V,Boyachek M,Fowler D B.A system for freezing biological materials.Horticulture Science,1978,13:171-172
[18]陈超,宋吉仁,王晓楠,付连双,谢冬微,李卓夫.寒地冬小麦抗寒性评价方法的初探.中国农学通报,2014,30(18):55-59Chen C,Song J R,Wang X N,Fu L S,Xie D W,Li ZF.Assessment Method of Cold-resistance on Winter Wheat.Chinese Agricultural Science Bulletin,2014,30(18):55-59
[19]Fowler D B,Gusta L V,Tyler N J.Selection for winter hardiness in wheat.Ⅲ.Screening methods.Crop Science,1981,5:896-901
[20]许瑛,陈发棣.菊花8个品种的低温半致死温度及其抗寒适应性.园艺学报,2008,35(4):559-564Xu Y,Chen F D.The LT50 and cold tolerance adaptability of chrysanthemum during a natural drop in temperature.Acta Horticulture Sinica,2008,35(4):559-564
[21]盖钧镒.试验统计方法.北京:中国农业出版社,2000:56-59Gai J Y.Test statistical method.Beijing:China Agriculture Press,2000:56-59
[22]Briggle L W,Curtis B C.Wheat worldwide.In wheat and wheat improvement.USA,1987:1-32
[23]皇甫自起,常守乾,李秀花,陈德华.豫东地区小麦冻害调查分析.河南农业科学,1996(4):3-6Huangfu Z Q,Chang S Q,Li X H,Chen D H.Investigation and analysis of wheat frost damage in the east of Henan province.Journal of Henan Agricultural Sciences,1996(4):3-6
[24]任永波,段拥军.作物抗寒性鉴定指标的分类及其应用.西昌农业高等专科学校学报,2002,16(2):43-46Ren Y B,Duan Y J.Classification and application of crop cold resistance identification index.Journal of Xichang Agricultural College,2002,16(2):43-46
[25]王红飞,李锡香,董洪霞,王海平,宋江萍,邱杨,张晓辉,沈镝.黄瓜核心种质芽期低温耐受性鉴定评价.植物遗传资源学报,2016,17(1):6-12Wang H F,Li X X,Dong H X,Wang H P,Song J P,Qiu Y,Zhang X H,Shen D.Cold tolerance identification at germination stage of cucumber core germplasm.Journal of Plant Genetic Resources,2016,17(1):6-12
[26]程嘉琪,沈镝,李锡香,王海平,邱杨,宋江萍.黄瓜核心种质低温耐受性的田间评价.植物遗传资源学报,2012,13(4):660-665Cheng J Q,Shen D,Li X X,Wang H P,Qiu Y,Song J P.Field evaluation of resistances to low temperature in core collections of cucumber germplasm.Journal of Plant Genetic Resources,2012,13(4):660-665
[27]柏斌,吴俊,庄文,姚栋萍,李莺歌,邓启云.广适性光温敏不育系Y58S幼穗分化期耐冷性表现及生理机制.植物遗传资源学报,2017,18(4):646-652Bai B,Wu J,Zhang W,Yao D P,Li Y G,Deng Q Y.Studies on cold tolerance of widely adaptable PTGMS line Y58S and its physiological mechanism at booting stage.Journal of Plant Genetic Resources,2017,18(4):646-652
[28]张丽华,梁双波,崔永增,张丽,姚海坡,贾秀领.黄淮海南部小麦抗寒性比较及指标筛选.中国农学通报,2014,30(12):77-81Zhang L H,Liang S B,Cui Y Z,Zhang L,Yao H P,Jia XL.Comparison of cold resistance in winter wheat and index selection in the south of Huanghuaihai.Chinese Agricultural Science Bulletin,2014,30(12):77-81
[29]刘方方.小麦抗寒相关基因克隆及分子标记开发.合肥:安徽农业大学,2016Liu F F.Cloning of cold-related resistance genes and molecular marker development in wheat.Hefei:Anhui Agricultural University,2016
[30]孙果忠,李海霞,雷秀玉,朱东旭,王海波.小麦品种耐寒性与春化VRN-1等位基因的关系研究.植物遗传资源学报,2013,14(2):270-277Sun G Z,Li H X,Lei X Y,Zhu D X,Wang H B.Relationship analysis between cold hardiness and vernalization VRN-1 alleles in wheat varieties.Journal of Plant Genetic Resources,2013,14(2):270-277
[31]王宝山.逆境植物生理学.北京:高等教育出版社,2010:26-30Wang B S.Adversity plant physiology.Beijing:Higher Education Press,2010:26-30
[2]李艳,王式功,马玉霞.全球气候变暖对我国小麦的影响研究综述.环境研究与检测,2006,19(2):11-13Li Y,Wang S G,Ma Y X.Review of the effects of global warming on Wheat in China.Environmental Study and Monitoring,2006,19(2):11-13
[3]沈漫,王明麻,黄敏仁.植物抗寒机理研究进展.植物学通报,1997,14(2):1-8Shen M,Wang M M,Huang M R.Advances in research on mechanism of cold resistance in plants.Chinese Bulletin of Botany,1997,14(2):1-8
[4]蒋志春,辛艳,吕志梅,周芳臣,李红梅,韩桂丽.冬小麦抗寒性级别划分与鉴定试验.天津农林科技,2005(1):7-8Jiang Z C,Xin Y,Lv Z M,Zhou F C,Li H M,Han GL.Classification and identification of winter wheat cold resistance.Science and Technology of Tianjin Agriculture and Forestry,2005(1):7-8
[5]刘艳阳,李俊周,陈磊,崔党群.低温胁迫对小麦叶片细胞膜脂质过氧化产物及相关酶活性的影响.麦类作物学报,2006,26(4):70-73Liu Y Y,Li J Z,Chen L,Cui D Q.Effect of low temperature stress on peroxidation product of membrane lipids and activity of related enzymes in wheat seedling leaves.Journal Triticeae Crops,2006,26(4):70-73
[6]Bohn M,Luthje S.Plasma membrane lipid alterations induced by cold acclimation and abscisic acid treatment of winter wheat seedlings differing in frost resistance.Plant Physiology,2007(164):146-156
[7]朱根海,刘祖祺,朱培仁.应用logistic方程确定植物组织低温半致死温度的研究.南京农业大学学报,1986,9(3):11-16Zhu G H,Liu Z Q,Zhu P R.Application of Logistic equation to determine the semi lethal temperature of plant tissues at low temperature.Journal of Nanjing Agricultural University,1986,9(3):11-16
[8]姜丽娜,张黛静,宋飞,刘佩,樊婷婷,余海波,李春喜.不同品种小麦叶片对拔节期低温的生理响应及抗寒性评价.生态学报,2014,34(15):4251-4261Jiang L N,Zhang D J,Song F,Liu P,Fan T T,Yu H B,Li CX.Evaluation of cold resistance of different wheat varieties based on physiological responses of leaves to low temperature at the jointing stage.Acta Ecologica Sinica,2014,34(15):4251-4261
[9]Yan L,Fu D M,Li C C,Blechl A,Tranquilli G,Bonafede M,Sanchez A,Valarik M,Yasuda S,Dubcovsky J.The wheat and barley vernalization gene Vrn3 is an orthologue of FT.Proceedings of the National Academy of Sciences,2006(103):19581-19586
[10]牟永潮,崔红,于晶,苍晶,曾俨,孟健男.低温胁迫下东农冬麦1号分蘖节SSH文库的构建及文库中3个基因的表达模式.作物学报,2011,37(5):918-923Mu Y C,Cui H,Yu J,Cang J,Zeng Y,Meng J N.Expression analysis of three genes from SSH library constructed using tillering nodes of Dongnongdongmai 1 under low temperature.Acta Agronomica Sinica,2011,37(5):918-923
[11]Badawi M,Danyluk J,Boucho B,Sarhan F.The CBFgene family in hexaploid wheat and its relationship to the phylogenetic complexity of cereal CBFs.Molecular Genetics and Genomics,2007,277(5):533-554
[12]Fowler D B,Limin A E,Wang S Y,Ward R W.Relationship between low-temperature tolerance and vernalization response in wheat and rye.Canadian Journal of Plant Science,1996,76(1):37-42
[13]Mastrangelo A M,Belloni S,Barilli S,Cattivelli L.Low temperature promotes intron retention in two ecor genes of durum wheat.Planta,2005,221(5):705-715
[14]赵玉田,梁博文.冬小麦抗冻性鉴定的方法指标及筛选.中国农业科学,1987,20(6):74-80Z h a o Y T,L i a n g B W.M e t h o d s a n d s c r e e n i n g o f t h e identification of winter wheat frost resistance.Scientia Agriculture Sinica,1987,20(6):74-80
[15]李卓夫,李莹,王晓楠.不同抗寒性冬小麦品种间冷冻后电导率与成活率比较分析.东北农业大学学报,2014,45(6):1-5Li Z F,Li Y,Wang X N.Comparative analysis of electrical conductivity and survival rate on winter wheat varieties with different cold resistances after freezing treatment.Journal of Northeast Agricultural University,2014,45(6):1-5
[16]申宗坦.作物育种学实验.北京:中国农业出版社,1992Shen Z T.Experiment of crop breeding.Beijing:China Agriculture Press,1992
[17]Gusta L V,Boyachek M,Fowler D B.A system for freezing biological materials.Horticulture Science,1978,13:171-172
[18]陈超,宋吉仁,王晓楠,付连双,谢冬微,李卓夫.寒地冬小麦抗寒性评价方法的初探.中国农学通报,2014,30(18):55-59Chen C,Song J R,Wang X N,Fu L S,Xie D W,Li ZF.Assessment Method of Cold-resistance on Winter Wheat.Chinese Agricultural Science Bulletin,2014,30(18):55-59
[19]Fowler D B,Gusta L V,Tyler N J.Selection for winter hardiness in wheat.Ⅲ.Screening methods.Crop Science,1981,5:896-901
[20]许瑛,陈发棣.菊花8个品种的低温半致死温度及其抗寒适应性.园艺学报,2008,35(4):559-564Xu Y,Chen F D.The LT50 and cold tolerance adaptability of chrysanthemum during a natural drop in temperature.Acta Horticulture Sinica,2008,35(4):559-564
[21]盖钧镒.试验统计方法.北京:中国农业出版社,2000:56-59Gai J Y.Test statistical method.Beijing:China Agriculture Press,2000:56-59
[22]Briggle L W,Curtis B C.Wheat worldwide.In wheat and wheat improvement.USA,1987:1-32
[23]皇甫自起,常守乾,李秀花,陈德华.豫东地区小麦冻害调查分析.河南农业科学,1996(4):3-6Huangfu Z Q,Chang S Q,Li X H,Chen D H.Investigation and analysis of wheat frost damage in the east of Henan province.Journal of Henan Agricultural Sciences,1996(4):3-6
[24]任永波,段拥军.作物抗寒性鉴定指标的分类及其应用.西昌农业高等专科学校学报,2002,16(2):43-46Ren Y B,Duan Y J.Classification and application of crop cold resistance identification index.Journal of Xichang Agricultural College,2002,16(2):43-46
[25]王红飞,李锡香,董洪霞,王海平,宋江萍,邱杨,张晓辉,沈镝.黄瓜核心种质芽期低温耐受性鉴定评价.植物遗传资源学报,2016,17(1):6-12Wang H F,Li X X,Dong H X,Wang H P,Song J P,Qiu Y,Zhang X H,Shen D.Cold tolerance identification at germination stage of cucumber core germplasm.Journal of Plant Genetic Resources,2016,17(1):6-12
[26]程嘉琪,沈镝,李锡香,王海平,邱杨,宋江萍.黄瓜核心种质低温耐受性的田间评价.植物遗传资源学报,2012,13(4):660-665Cheng J Q,Shen D,Li X X,Wang H P,Qiu Y,Song J P.Field evaluation of resistances to low temperature in core collections of cucumber germplasm.Journal of Plant Genetic Resources,2012,13(4):660-665
[27]柏斌,吴俊,庄文,姚栋萍,李莺歌,邓启云.广适性光温敏不育系Y58S幼穗分化期耐冷性表现及生理机制.植物遗传资源学报,2017,18(4):646-652Bai B,Wu J,Zhang W,Yao D P,Li Y G,Deng Q Y.Studies on cold tolerance of widely adaptable PTGMS line Y58S and its physiological mechanism at booting stage.Journal of Plant Genetic Resources,2017,18(4):646-652
[28]张丽华,梁双波,崔永增,张丽,姚海坡,贾秀领.黄淮海南部小麦抗寒性比较及指标筛选.中国农学通报,2014,30(12):77-81Zhang L H,Liang S B,Cui Y Z,Zhang L,Yao H P,Jia XL.Comparison of cold resistance in winter wheat and index selection in the south of Huanghuaihai.Chinese Agricultural Science Bulletin,2014,30(12):77-81
[29]刘方方.小麦抗寒相关基因克隆及分子标记开发.合肥:安徽农业大学,2016Liu F F.Cloning of cold-related resistance genes and molecular marker development in wheat.Hefei:Anhui Agricultural University,2016
[30]孙果忠,李海霞,雷秀玉,朱东旭,王海波.小麦品种耐寒性与春化VRN-1等位基因的关系研究.植物遗传资源学报,2013,14(2):270-277Sun G Z,Li H X,Lei X Y,Zhu D X,Wang H B.Relationship analysis between cold hardiness and vernalization VRN-1 alleles in wheat varieties.Journal of Plant Genetic Resources,2013,14(2):270-277
[31]王宝山.逆境植物生理学.北京:高等教育出版社,2010:26-30Wang B S.Adversity plant physiology.Beijing:Higher Education Press,2010:26-30