高温下黄瓜幼苗下胚轴长度遗传效应的研究
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摘要
本研究以下胚轴长度不同的黄瓜自交系‘GY2’(短下胚轴品系)ב平望乳瓜’(长下胚轴品系)为亲本构建了F1和F2,利用主基因+多基因混合遗传模型分析法研究了高温(43℃)下黄瓜幼苗下胚轴长度的遗传效应。结果表明:在高温和常温(25℃)下的F2分离群体中,黄瓜幼苗下胚轴长度均呈连续变异和正态分布,符合多基因控制的数量性状遗传规律;黄瓜幼苗在高温下的下胚轴长度符合2对加性-显性-上位性主基因+加性-显性多基因遗传模型,而常温下下胚轴长度则符合1对加性-显性主基因+加性-显性-上位性多基因遗传模型。研究结果可为黄瓜抗高温种质创新与新品种选育提供理论依据和实践指导。
I n this study, F1 and F2 were constructed from the parents of cucumber inbred lines 'GY2'(short hypocotyl) × 'Pingwangrugua'(long hypocotyl) with different hypocotyl length. The genetic effect of hypocotyl length of cucumber seedling in high temperature(43℃) were discussed using mixed major gene and polygene inheritance model. The results showed that the hypocotyl length of cucumber seedling maintained continuous variation and normal distribution in F2 segregation population under high temperature and normal temperature(25℃) situations, which fitted with the genetic regularity of quantitative traits controlled by polygenes. The hypocotyl length of cucumber seedling under high temperature conformed to the genetic model controlled by two pairs of additive-dominance-epistatic major genes with additive-dominant polygene. While the hypocotyls length under normal temperature conformed to the genetic model controlled by one pair of additive-dominant dominant genes with additive-dominant-epistatic polygenic genes. The results might provide theoretical basis and practical guidance for cucumber germplasm innovation of resistance to high temperature and new variety breeding.
I n this study, F1 and F2 were constructed from the parents of cucumber inbred lines 'GY2'(short hypocotyl) × 'Pingwangrugua'(long hypocotyl) with different hypocotyl length. The genetic effect of hypocotyl length of cucumber seedling in high temperature(43℃) were discussed using mixed major gene and polygene inheritance model. The results showed that the hypocotyl length of cucumber seedling maintained continuous variation and normal distribution in F2 segregation population under high temperature and normal temperature(25℃) situations, which fitted with the genetic regularity of quantitative traits controlled by polygenes. The hypocotyl length of cucumber seedling under high temperature conformed to the genetic model controlled by two pairs of additive-dominance-epistatic major genes with additive-dominant polygene. While the hypocotyls length under normal temperature conformed to the genetic model controlled by one pair of additive-dominant dominant genes with additive-dominant-epistatic polygenic genes. The results might provide theoretical basis and practical guidance for cucumber germplasm innovation of resistance to high temperature and new variety breeding.
引文
Cai Z.Z.,Chen X.Q.,Tang W.Q.,Li W.Q.,Wu W.R.,and Duan Y.L.,2016,Morphological characteristics,genetic analysis and gene location of two short panicle mutants of rice,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),35(5):1183-1188(蔡正正,陈学群,唐唯其,李文强,吴为人,段远霖,2016,两种水稻短穗突变体的形态特征、遗传分析和基因定位,基因组学与应用生物学,35(5):1183-1188)
Gai J.Y.,2005,Segregation analysis of genetic system of quantitative traits in plants,Yichuan(Hereditas),27(1):130-136(盖均镒,2005,植物数量性状遗传体系的分离分析方法研究,遗传,27(1):130-136)
Gai J.Y.,Zhang Y.M.,and Wang J.K.,eds.,2003,Genetic system of quantitative traits in plants,Science Press,Beijing,China,pp.224-265(盖均镒,章元明,王建康,编著,2003,植物数量性状遗传体系,科学出版社,中国,北京,pp.224-265)
Gao X.X.,2011,Study on the mechanism and measures of controlling hypocotyls overgrowth of cucumber and tomato seedlings,Thesis for M.S.,Chinese Academy of Agricultural Sciences,Supervisor:Shang Q.M.,pp.1-3(高晓旭,2011,黄瓜,番茄幼苗下胚轴徒长制御及作用机理,硕士学位论文,中国农业科学院,导师:尚庆茂,pp.1-3)
Gu X.F.,Zhang C.Z.,Feng L.L.,Fang X.J.,and Zhang T.M.,2002,Genetic analysis of cucumber seedling characters in protected area,Yuanyi Xuebao(Acta Horticulturae Sinica),29(5):443-446(顾兴芳,张春震,封林林,方秀娟,张天明,2002,保护地黄瓜苗期性状遗传分析,园艺学报,29(5):443-446)
Jian X.,Miao Y.M.,Gao Q.H.,and Sui Y.H.,2015,Analysis of mixed major-gene with polygenes genetic model for fruit shape traits in cucumber,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),13(10):2262-2267(简兴,苗永美,高青海,隋益虎,2015,黄瓜果形性状主基因+多基因混合遗传分析,分子植物育种,13(10):2262-2267)
Li D.D.,Si L.T.,Luo X.M.,and Li T.,2009,Genetic analysis on hypocotyl traits of cucumber seedlings under low light stress,Xibei Nonglin Keji Daxue Xuebao(Journal of Northwest A&F University(Natural Science Edition)),37(11):113-119(李丹丹,司龙亭,罗晓梅,李涛,2009,弱光胁迫下黄瓜苗期下胚轴性状的遗传分析,西北农林科技大学学报(自然科学版),37(11):113-119)
Ming C.H.,Jiang F.L.,Hu H.M.,Zhou X.C.,Zhan F.H.,and Wu Z.,2011,Effects of different leggy extent seedlings on cucumber growth,yield and quality,Zhongguo Shucai(China Vegetables),1(4):29-34(明村豪,蒋芳玲,胡宏敏,周学超,詹锋华,吴震,2011,幼苗徒长程度对黄瓜植株生长发育及产量品质的影响,中国蔬菜,1(4):29-34)
Nie J.T.,Pan J.S.,He H.L.,Si L.T.,and Cai R.,2011,SSR inheritance analysis and screening for linked marker of powdery mildew resistance in cucumber(Cucumis sativus L.),Zhongguo Shucai(China Vegetables),1(10):45-49(聂京涛,潘俊松,何欢乐,司龙亭,蔡润,2011,黄瓜白粉病抗性遗传分析与连锁标记筛选,中国蔬菜,1(10):45-49)
Peleman J.D.,Wye C.,Zethof J.,S覬rensen A.P.,Verbakel H.,Van Oeveren J.,Gerats T.,and Van Der Voort J.R.,2005,Quantitative trait locus(QTL)is ogenic recombinant analysis:a method for high-resolution mapping of QTL within a single population,Genetics Society of America,171(3):1341-1352
Shen D.,Fang Z.Y.,Li X.X.,Li Q.H.,Cheng J.Q.,Song J.P.,Wang H.P.,and Qiu Y.,2011,Inheritance of fruit flesh colorin Cucumis sativus L.,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),12(2):216-222(沈镝,方智远,李锡香,李全辉,程嘉琪,宋江萍,王海平,邱杨,2011,黄瓜果肉色的遗传分析,植物遗传资源学报,12(2):216-222)
Xu Q.,Chen Y.G.,Qi X.H.,and Chen X.H.,2014,Genetic analysis of fructose content with mixed model of major gene plus polygene in cucumber,Yangzhou Daxue Xuebao(Journal of Yangzhou University(Agricultural and Life Science Edition)),35(2):86-89,94(徐强,陈银根,齐晓花,陈学好,2014,黄瓜果糖含量的主基因-多基因遗传分析,扬州大学学报(农业与生命科学版),35(2):86-89,94)
Yang X.,Liu F.,Zhang Y.,Cheng Y.F.,Xue L.B.,and Chen X.H.,2016,Study on the genetic diversity of eggplant germplasm with SSR markers,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),35(12):3450-3457(杨旭,刘飞,张宇,成玉富,薛林宝,陈学好,2016,利用SSR标记研究茄子种质资源遗传多样性,基因组学与应用生物学,35(12):3450-3457)
Zhang L.,Si L.T.,and Li K.,2012,Genetic analysis of fruit length in cucumber,Xibei Nongye Xuebao(Acta Agriculturae Boreali-occidentalis Sinica),21(3):114-117,147(张磊,司龙亭,李坤,2012,黄瓜瓜条长度的遗传分析,西北农业学报,21(3):114-117,147)
Zhang Y.M.,2006,Advances on methods for mapping QTL in plant,Zhongguo Kexue Tongbao(Chinese Science Bulletin),51(23):2809-2818(章元明,2006,植物QTL定位方法研究进展,中国科学通报,51(23):2809-2818)
Zhang Y.M.,Gai J.Y.,and Zhang M.C.,2000,Jointly segregating analysis of P1P2F1and F2OR F2∶3families,Xinan Nongye Daxue Xuebao(Journal of southwest Agricultural University),22(1):6-9(章元明,盖均镒,张孟臣,2000,利用P1P2F1和F2或F2∶3世代联合的数量性状分离分析,西南农业大学学报,22(1):6-9)
Zhao Y.,Shen J.,Li J.,Zhang L.,Lou Q.F.,and Chen J.F.,2017,Genetic of seed-cavity color traits in cucumber(Cucumis sativus L.),Fengzi Zhiwu Yuzhong(Molecular Plant Breeding),15(4):1478-1484(赵宇,沈佳,李季,张璐,娄群峰,陈劲枫,2017,黄瓜心腔颜色的遗传,分子植物育种,15(4):1478-1484)
Gai J.Y.,2005,Segregation analysis of genetic system of quantitative traits in plants,Yichuan(Hereditas),27(1):130-136(盖均镒,2005,植物数量性状遗传体系的分离分析方法研究,遗传,27(1):130-136)
Gai J.Y.,Zhang Y.M.,and Wang J.K.,eds.,2003,Genetic system of quantitative traits in plants,Science Press,Beijing,China,pp.224-265(盖均镒,章元明,王建康,编著,2003,植物数量性状遗传体系,科学出版社,中国,北京,pp.224-265)
Gao X.X.,2011,Study on the mechanism and measures of controlling hypocotyls overgrowth of cucumber and tomato seedlings,Thesis for M.S.,Chinese Academy of Agricultural Sciences,Supervisor:Shang Q.M.,pp.1-3(高晓旭,2011,黄瓜,番茄幼苗下胚轴徒长制御及作用机理,硕士学位论文,中国农业科学院,导师:尚庆茂,pp.1-3)
Gu X.F.,Zhang C.Z.,Feng L.L.,Fang X.J.,and Zhang T.M.,2002,Genetic analysis of cucumber seedling characters in protected area,Yuanyi Xuebao(Acta Horticulturae Sinica),29(5):443-446(顾兴芳,张春震,封林林,方秀娟,张天明,2002,保护地黄瓜苗期性状遗传分析,园艺学报,29(5):443-446)
Jian X.,Miao Y.M.,Gao Q.H.,and Sui Y.H.,2015,Analysis of mixed major-gene with polygenes genetic model for fruit shape traits in cucumber,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),13(10):2262-2267(简兴,苗永美,高青海,隋益虎,2015,黄瓜果形性状主基因+多基因混合遗传分析,分子植物育种,13(10):2262-2267)
Li D.D.,Si L.T.,Luo X.M.,and Li T.,2009,Genetic analysis on hypocotyl traits of cucumber seedlings under low light stress,Xibei Nonglin Keji Daxue Xuebao(Journal of Northwest A&F University(Natural Science Edition)),37(11):113-119(李丹丹,司龙亭,罗晓梅,李涛,2009,弱光胁迫下黄瓜苗期下胚轴性状的遗传分析,西北农林科技大学学报(自然科学版),37(11):113-119)
Ming C.H.,Jiang F.L.,Hu H.M.,Zhou X.C.,Zhan F.H.,and Wu Z.,2011,Effects of different leggy extent seedlings on cucumber growth,yield and quality,Zhongguo Shucai(China Vegetables),1(4):29-34(明村豪,蒋芳玲,胡宏敏,周学超,詹锋华,吴震,2011,幼苗徒长程度对黄瓜植株生长发育及产量品质的影响,中国蔬菜,1(4):29-34)
Nie J.T.,Pan J.S.,He H.L.,Si L.T.,and Cai R.,2011,SSR inheritance analysis and screening for linked marker of powdery mildew resistance in cucumber(Cucumis sativus L.),Zhongguo Shucai(China Vegetables),1(10):45-49(聂京涛,潘俊松,何欢乐,司龙亭,蔡润,2011,黄瓜白粉病抗性遗传分析与连锁标记筛选,中国蔬菜,1(10):45-49)
Peleman J.D.,Wye C.,Zethof J.,S覬rensen A.P.,Verbakel H.,Van Oeveren J.,Gerats T.,and Van Der Voort J.R.,2005,Quantitative trait locus(QTL)is ogenic recombinant analysis:a method for high-resolution mapping of QTL within a single population,Genetics Society of America,171(3):1341-1352
Shen D.,Fang Z.Y.,Li X.X.,Li Q.H.,Cheng J.Q.,Song J.P.,Wang H.P.,and Qiu Y.,2011,Inheritance of fruit flesh colorin Cucumis sativus L.,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),12(2):216-222(沈镝,方智远,李锡香,李全辉,程嘉琪,宋江萍,王海平,邱杨,2011,黄瓜果肉色的遗传分析,植物遗传资源学报,12(2):216-222)
Xu Q.,Chen Y.G.,Qi X.H.,and Chen X.H.,2014,Genetic analysis of fructose content with mixed model of major gene plus polygene in cucumber,Yangzhou Daxue Xuebao(Journal of Yangzhou University(Agricultural and Life Science Edition)),35(2):86-89,94(徐强,陈银根,齐晓花,陈学好,2014,黄瓜果糖含量的主基因-多基因遗传分析,扬州大学学报(农业与生命科学版),35(2):86-89,94)
Yang X.,Liu F.,Zhang Y.,Cheng Y.F.,Xue L.B.,and Chen X.H.,2016,Study on the genetic diversity of eggplant germplasm with SSR markers,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),35(12):3450-3457(杨旭,刘飞,张宇,成玉富,薛林宝,陈学好,2016,利用SSR标记研究茄子种质资源遗传多样性,基因组学与应用生物学,35(12):3450-3457)
Zhang L.,Si L.T.,and Li K.,2012,Genetic analysis of fruit length in cucumber,Xibei Nongye Xuebao(Acta Agriculturae Boreali-occidentalis Sinica),21(3):114-117,147(张磊,司龙亭,李坤,2012,黄瓜瓜条长度的遗传分析,西北农业学报,21(3):114-117,147)
Zhang Y.M.,2006,Advances on methods for mapping QTL in plant,Zhongguo Kexue Tongbao(Chinese Science Bulletin),51(23):2809-2818(章元明,2006,植物QTL定位方法研究进展,中国科学通报,51(23):2809-2818)
Zhang Y.M.,Gai J.Y.,and Zhang M.C.,2000,Jointly segregating analysis of P1P2F1and F2OR F2∶3families,Xinan Nongye Daxue Xuebao(Journal of southwest Agricultural University),22(1):6-9(章元明,盖均镒,张孟臣,2000,利用P1P2F1和F2或F2∶3世代联合的数量性状分离分析,西南农业大学学报,22(1):6-9)
Zhao Y.,Shen J.,Li J.,Zhang L.,Lou Q.F.,and Chen J.F.,2017,Genetic of seed-cavity color traits in cucumber(Cucumis sativus L.),Fengzi Zhiwu Yuzhong(Molecular Plant Breeding),15(4):1478-1484(赵宇,沈佳,李季,张璐,娄群峰,陈劲枫,2017,黄瓜心腔颜色的遗传,分子植物育种,15(4):1478-1484)