代谢组学在动物应激研究中的应用
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摘要
代谢组学是生物机体在病理生理、遗传、环境或行为的不同刺激下,对机体所产生代谢产物变化的研究,是生物领域研究中一种重要的研究手段,已在植物学、营养学、中药学、微生物学、临床疾病诊断等研究领域得到广泛应用。动物应激一直是畜牧行业关注的热点,许多因素可导致动物应激,常见的应激类型有冷应激、热应激、氧化应激、束缚应激、噪音应激、运输应激,还有不可预知的慢性应激等,这些应激均可对动物机体产生一定危害。目前,代谢组学也已应用于动物应激领域的研究,本文对代谢组学在动物的冷应激、热应激、氧化应激及束缚应激研究中的应用作一综述。
Metabolomics is a subject about the change of metabolite of organisms stimulated by pathophysiological,genetic,environmental or behavior factors,which is an important method for biological research,and has been widely applied in the fields of botany,nutrition,Chinese pharmacy,microbiology and clinical disease diagnosis. Animal stress is a hotpot which is paid attention in animal husbandry,including cold stress,heat stress,oxidative stress,restraint stress,noise stress,transport stress and unpredictable chronic stress,which is caused by multiple factors and has a certain extent of harm to animals. At present,metabolomics is also applied in the field of animal stress. This article describes the application of metabolomics in the researches on cold stress,heat stress,oxidative stress and restraint stress in animals.
Metabolomics is a subject about the change of metabolite of organisms stimulated by pathophysiological,genetic,environmental or behavior factors,which is an important method for biological research,and has been widely applied in the fields of botany,nutrition,Chinese pharmacy,microbiology and clinical disease diagnosis. Animal stress is a hotpot which is paid attention in animal husbandry,including cold stress,heat stress,oxidative stress,restraint stress,noise stress,transport stress and unpredictable chronic stress,which is caused by multiple factors and has a certain extent of harm to animals. At present,metabolomics is also applied in the field of animal stress. This article describes the application of metabolomics in the researches on cold stress,heat stress,oxidative stress and restraint stress in animals.
引文
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[15]BERNABUCCI U,BIFFANI S,BUGGIOTTI L,et al.The effects of heat stress in Italian Holstein dairy cattle[J].J Dairy Sci,2014,97(1):471-486.
[16]LIAO Y,HU R,WANG Z,et al.Metabolomics profiling of serum and urine in three beef cattle breeds revealed different levels of tolerance to heat stress[J].J Agric Food Chem,2018,66(26):6926-6935.
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[18]WANG L,URRIOLA P E,LUO Z H,et al.Metabolomics revealed diurnal heat stress and zinc supplementation-induced changes in amino acid,lipid,and microbial metabolism[J].Physiol Rep,2016,4(1):e12676.doi:10.14814/phy2.12676.
[19]PAVLIDES S,TSIRIGOS A,MIGNECO G,et al.The autophagic tumor stroma model of cancer:Role of oxidative stress and ketone production in fueling tumor cell metabolism[J].Cell Cycle,2010,9(17):3485-3505.
[20]CULOTTA V C.Superoxide dismutase,oxidative stress,and cell metabolism[J].Curr Top Cell Regul,2000,36(1):117-132.
[21]RANGEL-HUERTA O D,AGUILERA C M,PEREZ-DE-LACRUZ A,et al.A serum metabolomics-driven approach predicts orange juice consumption and its impact on oxidative stress and inflammation in subjects from the BIONAOS study[J].Mol Nutr Food Res,2017,61(2).doi:10.1002/mnfr.201600120.
[22]LIU W,JIN F,GAO D,et al.Metabolomics analysis reveals aminoquinazolin derivative 9d-induced oxidative stress and cell cycle arrest in A549 cells[J].Rsc Adv,2017,7(22):13149-13158.
[23]PAN X,WANG K,LU C,et al.Effects of the chronic restraint stress induced depression on reward-related learning in rats[J].Behav Brain Res,2017,321(3):185-192.
[24]ZHENG X Y,GAO X X,LIU X J,et al.Evaluation on stress animal models of depression based on metabonomics[J].Drug Eval Res,2010,33(3):175-179.(in Chinese)郑兴宇,高晓霞,刘晓节,等.基于代谢组学技术应激抑郁动物模型的评价[J].药物评价研究,2010,33(3):175-179.
[25]LIU L,ZHOU X,ZHANG Y,et al.The identification of metabolic disturbances in the prefrontal cortex of the chronic restraint stress rat model of depression[J].Behav Brain Res,2016,305(5):148-156.
[26]LUO H G,CHEN J X,ZHANG Q,et al.Comparative study on effects of Xiaoyao Powder and its modified prescription on plasma metabolomics of rats with chronic immobilization stress[J].Chin J Integr Med,2013,19(8):610-615.
[27]YOU R,PANG Q,LI L.A metabolic phenotyping approach to characterize the effects of cantonese herbal tea on restraint stressed rats[J].Biol Pharm Bull,2014,37(9):1466-1474.
[2]LINDON J C,NICHOLSON J K,HOLMES E,et al.Contemporary issues in toxicology the role of metabonomics in toxicology and its evaluation by the COMET project[J].Toxicol Appl Pharmacol,2003,187(3):137-146.
[3]SUN L W,武江利.基于H-1核磁共振的临床和亚临床酮病奶牛血浆代谢组学研究[J].中国畜牧兽医,2014,41(12):73-73.
[4]WILLIAMS R E,LENZ E M,RANTALAINEN M,et al.The comparative metabonomics of age-related changes in the urinary composition of male Wistar-derived and Zucker(fa/fa)obese rats[J].Mol Biosyst,2006,2(3-4):193-202.
[5]MEDINA-GOMEZ G,GRAY S L,YETUKURI L,et al.PPAR gamma 2 prevents lipotoxicity by controlling adipose tissue expandability and peripheral lipid metabolism[J].PLo S Genet,2007,3(4):e64.
[6]WU G P,WANG R R,TIAN H,et al.Metabonomics study of allergy animal model[J].China J Mod Med,2011,21(23):2847-2850.(in Chinese)吴公平,王蓉蓉,田洪,等.过敏反应动物模型的代谢组学研究[J].中国现代医学杂志,2011,21(23):2847-2850.
[7]YU H,XIU J J,MA B,et al.Preliminary studies of the CUMS animal models with metabonomics based nuclear magnetic resonance[J].J Med Imaging,2015,25(12):2240-2244.(in Chinese)余红,修建军,马彬,等.基于核磁共振的代谢组学方法对CUMS动物模型的初步研究[J].医学影像学杂志,2015,25(12):2240-2244.
[8]JI H,LIU J X,YANG H M,et al.Research progress on effects of cold stress on main neuroendocrine responses[J].J Environ Health,2012,29(5):470-472.(in Chinese)计红,柳巨雄,杨焕民,等.冷应激对动物神经内分泌系统影响的研究进展[J].环境与健康杂志,2012,29(5):470-472.
[9]GUO J R,YANG H M,WANG Z W,et al.Research progress on effects of cold stress on function of immune system in cattle and poultry[J].J Environ Health,2010,27(7):649-651.(in Chinese)郭景茹,杨焕民,王忠伟,等.冷应激对畜禽免疫系统功能影响的研究进展[J].环境与健康杂志,2010,27(7):649-651.
[10]李静,李玉恒,孟宇,等.冷应激对动物相关基因表达的影响[C].全国动物生理生化第十二次学术交流会论文摘要汇编.2012.
[11]LU H,ULANOV A V,NOBU M,et al.Global metabolomic responses of Nitrosomonas europaea 19718 to cold stress and altered ammonia feeding patterns[J].Appl Microbiol Biotechnol,2016,100(4):1843-1852.
[12]ZHU Y C,YOCOM E,SIFERS J,et al.Modulatory effects on Drosophila,larva hearts:room temperature,acute and chronic cold stress[J].J Comp Physiol B,2016,186(7):829-841.
[13]LU X,SOLMONSON A,LODI A,et al.The early metabolomic response of adipose tissue during acute cold exposure in mice[J].Sci Rep,2017,7(1):3455.
[14]WANG Y L,HAN Z H,ZHENG L Y,et al.Effects of Herba Rhodiolae sacrae decoction on plasma and urine metabonome of rat model induced by chronic cold stress[J].Trad Chin Drug Res&Clin Pharmacol,2016,27(1):75-80.(in Chinese)王亚玲,韩智慧,郑凌云,等.红景天对慢性冷应激大鼠模型血浆和尿液代谢组的影响[J].中药新药与临床药理,2016,27(1):75-80.
[15]BERNABUCCI U,BIFFANI S,BUGGIOTTI L,et al.The effects of heat stress in Italian Holstein dairy cattle[J].J Dairy Sci,2014,97(1):471-486.
[16]LIAO Y,HU R,WANG Z,et al.Metabolomics profiling of serum and urine in three beef cattle breeds revealed different levels of tolerance to heat stress[J].J Agric Food Chem,2018,66(26):6926-6935.
[17]黄毅娜,邓鹏翅,陈槐萱,等.高温对SD大鼠致畸试验羊水1H-NMR代谢组分析[C].中国环境诱变剂学会风险评价专业委员会全国学术交流会.宁夏银川,2014.
[18]WANG L,URRIOLA P E,LUO Z H,et al.Metabolomics revealed diurnal heat stress and zinc supplementation-induced changes in amino acid,lipid,and microbial metabolism[J].Physiol Rep,2016,4(1):e12676.doi:10.14814/phy2.12676.
[19]PAVLIDES S,TSIRIGOS A,MIGNECO G,et al.The autophagic tumor stroma model of cancer:Role of oxidative stress and ketone production in fueling tumor cell metabolism[J].Cell Cycle,2010,9(17):3485-3505.
[20]CULOTTA V C.Superoxide dismutase,oxidative stress,and cell metabolism[J].Curr Top Cell Regul,2000,36(1):117-132.
[21]RANGEL-HUERTA O D,AGUILERA C M,PEREZ-DE-LACRUZ A,et al.A serum metabolomics-driven approach predicts orange juice consumption and its impact on oxidative stress and inflammation in subjects from the BIONAOS study[J].Mol Nutr Food Res,2017,61(2).doi:10.1002/mnfr.201600120.
[22]LIU W,JIN F,GAO D,et al.Metabolomics analysis reveals aminoquinazolin derivative 9d-induced oxidative stress and cell cycle arrest in A549 cells[J].Rsc Adv,2017,7(22):13149-13158.
[23]PAN X,WANG K,LU C,et al.Effects of the chronic restraint stress induced depression on reward-related learning in rats[J].Behav Brain Res,2017,321(3):185-192.
[24]ZHENG X Y,GAO X X,LIU X J,et al.Evaluation on stress animal models of depression based on metabonomics[J].Drug Eval Res,2010,33(3):175-179.(in Chinese)郑兴宇,高晓霞,刘晓节,等.基于代谢组学技术应激抑郁动物模型的评价[J].药物评价研究,2010,33(3):175-179.
[25]LIU L,ZHOU X,ZHANG Y,et al.The identification of metabolic disturbances in the prefrontal cortex of the chronic restraint stress rat model of depression[J].Behav Brain Res,2016,305(5):148-156.
[26]LUO H G,CHEN J X,ZHANG Q,et al.Comparative study on effects of Xiaoyao Powder and its modified prescription on plasma metabolomics of rats with chronic immobilization stress[J].Chin J Integr Med,2013,19(8):610-615.
[27]YOU R,PANG Q,LI L.A metabolic phenotyping approach to characterize the effects of cantonese herbal tea on restraint stressed rats[J].Biol Pharm Bull,2014,37(9):1466-1474.