新疆昭苏县马泰勒虫病的诊疗及其效果观察
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摘要
为了观察和探讨不同检测方法及不同药物对马泰勒虫病的临床诊疗效果,提高对马泰勒虫病临床诊疗的防治水平,随机采集昭苏县种马场128匹疑似马血样品,采用临床症状观察、病原学检查和PCR检测等方法确诊了12例马泰勒虫病病例。将12匹阳性马随机分为2组,即处方1组和处方2组,用药后的5d内,分别对每组患马进行临床症状观察、体温测定、血涂片检查和染虫率统计。结果显示,血涂片中含有圆形或圆点形的马泰勒虫,PCR检测得到512bp的目的条带,与目的片段相符。第1治疗组(三氮脒治疗组)治愈率为66.7%(4/6),第2治疗组(盐酸吖啶黄组)治愈率为83.3%(5/6),第2治疗组治愈率高于第1治疗组。本试验发现,临床症状观察、病原学检查和PCR检测法相结合可准确诊断马泰勒虫病;处方2组是2种处方中治疗马泰勒虫病的最佳处方,以上诊疗数据可为马泰勒虫病的防控提供参考。
To observe and explore the effects of different detection methods and drugs on the clinical diagnosis and treatment in horses infected with Theileria equi,to raise awareness of the clinical diagnosis and treatment and prevention level,one hundered and twenty-eight suspected horse blood samples collected from Zhaosu county stud farm randomly.12 horses infected with Theileria equi were confirmed by clinical symptoms,etiology examination and PCR detection methods.The 12 positive horses were randomly divided into two groups named the prescription group 1 and the prescription group 2,within 5 days after treatment,respectively,for each group of horses,the clinical symptoms,temperature,blood smears and infestation rate of erythrocytes were recorded.Results showed that there are round or round point shaped Theileria equi in erythrocytes.PCR detection obtained a 512 bp band which matched purpose fragment.Nitrogen amidine first treatment group cure rate was 66.7% (4/6),Hydrochloric acid(second group)cure rate was 83.3% (5/6),the cure rate of the second treatment group is higher than that of the first treatment group.This study found that combination of clinical symptom observation,etiology examination and PCR detection method can accurately diagnose Theileria equi infection,the prescription group 2 was better for the treatment of Theileria equi infection in these two prescriptions.These clinical data can lay a foundation for prevention and control of Theileria equi infection.
To observe and explore the effects of different detection methods and drugs on the clinical diagnosis and treatment in horses infected with Theileria equi,to raise awareness of the clinical diagnosis and treatment and prevention level,one hundered and twenty-eight suspected horse blood samples collected from Zhaosu county stud farm randomly.12 horses infected with Theileria equi were confirmed by clinical symptoms,etiology examination and PCR detection methods.The 12 positive horses were randomly divided into two groups named the prescription group 1 and the prescription group 2,within 5 days after treatment,respectively,for each group of horses,the clinical symptoms,temperature,blood smears and infestation rate of erythrocytes were recorded.Results showed that there are round or round point shaped Theileria equi in erythrocytes.PCR detection obtained a 512 bp band which matched purpose fragment.Nitrogen amidine first treatment group cure rate was 66.7% (4/6),Hydrochloric acid(second group)cure rate was 83.3% (5/6),the cure rate of the second treatment group is higher than that of the first treatment group.This study found that combination of clinical symptom observation,etiology examination and PCR detection method can accurately diagnose Theileria equi infection,the prescription group 2 was better for the treatment of Theileria equi infection in these two prescriptions.These clinical data can lay a foundation for prevention and control of Theileria equi infection.
引文
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[13]李佳.马泰勒虫荧光定量PCR检测方法的建立及伊犁马泰勒虫病的分子流行病学调查[D].新疆乌鲁木齐:新疆农业大学,2014.
[14]李文志.马梨形虫病的诊断与治疗[J].青海畜牧兽医杂志,1999,26(3):48.
[15]魏锦龙,李昌铭.马类巴贝斯虫病的防治[J].中兽医学杂志,2013,40(6):35.
[2]Micaela S,Francesca B,Simona N,et al.Seroprevalence and molecular analysis of Babesia caballi and Theileriaequi in horses from central Italy during a 10-year period[J].J Equi Vet Sci,2015,35(10):865-868.
[3]Salim B O M,Hassan S M,Bakheit M A,et al.Diagnosis of Babesia caballi and Theileria equi infections in horses in Sudan using ELISA and PCR[J].Parasitol Res,2008,103(5):1145-1150.
[4]孔繁瑶.家畜寄生虫学[M].2版.北京:中国农业大学出版社,2010:330-331.
[5]瓦热斯·吐尔松,李永畅,朱玉涛,等.新疆昌吉部分地区马梨形虫病检测初报[J].中国动物传染病学报,2016,23(1):75-79.
[6]罗金,刘光远,田占成,等.马泰勒虫EMA1基因的克隆与生物学特性分析[J].中国兽医科学,2012,42(4):352-356.
[7]姜新基,六十,布仁巴依尔,等.马焦虫病的临床症状及防治措施[J].中国畜禽种业,2014,21(10):82-83.
[8]孙晓华.牛、马巴贝斯虫病的诊断与防治[J].养殖技术顾问,2014,42(2):176.
[9]闫常平,薛克云,安海军.马巴贝斯虫病的暴发与诊治[J].中国兽医杂志,2003,51(9):52-53.
[10]马萍.马类家畜巴贝斯虫病的诊治[J].甘肃畜牧兽医,2005(4):30-31.
[11]王蕾,刘亚萍,张大红.三种血涂片染色方法在教学应用中的比较[J].实用医技杂志,2015(9):984-985.
[12]罗金,刘光远,谢俊仁,等.马泰勒虫病PCR检测方法的建立和应用[J].中国畜牧兽医,2012,41(2):28-31.
[13]李佳.马泰勒虫荧光定量PCR检测方法的建立及伊犁马泰勒虫病的分子流行病学调查[D].新疆乌鲁木齐:新疆农业大学,2014.
[14]李文志.马梨形虫病的诊断与治疗[J].青海畜牧兽医杂志,1999,26(3):48.
[15]魏锦龙,李昌铭.马类巴贝斯虫病的防治[J].中兽医学杂志,2013,40(6):35.