马副蛔虫卵的鉴定及玻片标本的制作
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摘要
为了探索马副蛔虫虫卵玻片永久性标本制作的理想方法,提供优质的标本资源,提高实验课教学的质量,笔者收集驱虫后的普氏野马马副蛔虫(雌),借助手术刀片切开其腹腔,取出子宫,离心沉淀后收集虫卵,取适量的虫卵与甲醛中混匀,制作玻片标本。结果表明,驱虫前后马副蛔虫感染率分别为87.50%和36.84%,感染强度(平均EPG)为128.6和43.6;制作的虫卵标本能准确显示蛔虫卵的原始形态,其形态特征保持原状,能克服气泡、发霉、变形等问题,具有经济实惠、能长久保存等特点。
To explore an ideal preparation technique of permanent slide specimens for egg of Parascaris equorum for providing the high quality samples and enhance the quality of experimental teaching.Female Parascaris equorum samples were collected after expelling parasite,dissected the Parascaris equorum and remove the uterus and collected eggs by centrifuge.Mixing the eggs and formaldehyde and then make the specimens.The result showed that before expelling parasite,the infection rate of Parascaris equorum was 87.5%,and after expelling parasite the infection rate was 36.84%,insect repellent before and after the EPG were 128.6 and 43.6,respectively.The egg specimens could display the natural form of egg of Parascaris equorum and the merphological characteristics could remain unchanged.Furthermore,the specimens can overcome many problems like bubble、mildew and deformation and have the advantages of economical benefit and long-term preservation at the same time.
To explore an ideal preparation technique of permanent slide specimens for egg of Parascaris equorum for providing the high quality samples and enhance the quality of experimental teaching.Female Parascaris equorum samples were collected after expelling parasite,dissected the Parascaris equorum and remove the uterus and collected eggs by centrifuge.Mixing the eggs and formaldehyde and then make the specimens.The result showed that before expelling parasite,the infection rate of Parascaris equorum was 87.5%,and after expelling parasite the infection rate was 36.84%,insect repellent before and after the EPG were 128.6 and 43.6,respectively.The egg specimens could display the natural form of egg of Parascaris equorum and the merphological characteristics could remain unchanged.Furthermore,the specimens can overcome many problems like bubble、mildew and deformation and have the advantages of economical benefit and long-term preservation at the same time.
引文
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[4]马元芬,张曦.寄生虫永久性混合虫卵制片方法的改进[J].黔南民族医专学报,2001,14(3):188.
[5]刘锦华,张丰雪.蠕虫卵玻片标本制作方法的比较[J].泰山医学院学报,2001,22(2):151.
[6]李佳,李永畅,冉多良,等.新源县孕马蠕虫体内滞留情况调查[J].新疆农业科学,2013,50(3):553-556.
[7]图尔荪·萨迪尔,卡丽比努尔·尔肯,朱玉涛,等.新疆阿勒泰富蕴县马自然感染消化道线虫情况初报[J].中国动物传染病学报,2015,23(3):69-72.