云南省红河州马梨形虫病血清学检测
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摘要
为了解云南边境地区马梨形虫病流行情况,2017年1—2月,随机采集红河州泸西县、弥勒市、个旧市、开远市4个地区27个乡(镇)农户饲养的马属动物血样462份,采用ELISA双抗原夹心法检测马梨形虫病抗体。结果显示:共检出4份阳性血样,平均样品阳性率为0.87%;阳性样本分布于弥勒市和个旧市4个乡(镇)年龄为7～13岁的个体中;不同地区、年龄、动物种类之间的阳性率均没有明显差异(P>0.05)。调查结果表明,该地存在马梨形虫病病原。这需引起当地重视,尤其在蚊虫活跃季节,更要做好防蜱和灭蜱工作。
In order to recognize the prevalence status of equine piroplasmosis in Yunnan border area,during January to February of 2017,462 serum samples of equine animals were collected randomly from 27 villages(townships)of 4 districts including Luxi,Mile,Gejiu and Kaiyuan,then double antigen sandwich ELISA method was used for detecting antibodies against equine piroplasmosis. Results showed that 4 blood samples were detected positive,the average positive rate was 0.87%;these positive samples were distributed among individuals of 7 to 13 years old in 4 villages(townships),there was no obvious difference(P>0.05)in positive rate between the different districts,ages and animal species. As a conclusion,equine piroplasmosis existed in Honghe Autonomous Prefecture of Yunnan Province,which should be paid attention by local government. In mosquito seasons,preventing,controlling and killing ticks should be strengthened.
In order to recognize the prevalence status of equine piroplasmosis in Yunnan border area,during January to February of 2017,462 serum samples of equine animals were collected randomly from 27 villages(townships)of 4 districts including Luxi,Mile,Gejiu and Kaiyuan,then double antigen sandwich ELISA method was used for detecting antibodies against equine piroplasmosis. Results showed that 4 blood samples were detected positive,the average positive rate was 0.87%;these positive samples were distributed among individuals of 7 to 13 years old in 4 villages(townships),there was no obvious difference(P>0.05)in positive rate between the different districts,ages and animal species. As a conclusion,equine piroplasmosis existed in Honghe Autonomous Prefecture of Yunnan Province,which should be paid attention by local government. In mosquito seasons,preventing,controlling and killing ticks should be strengthened.
引文
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[2]CANTú-MARTíNEZ M A,SEGURA-CORREA J C,SILVA-PáEZ M L,et al.Prevalence of antibodies to Theileria equi and Babesia caballi in horses from northeastern Mexico[J].Journal of parasitology,2012,98(4):869-870.
[3]罗金,刘光远,谢俊仁,等.驽梨形虫病PCR检测方法的建立和应用[J].中国畜牧兽医,2011,38(7):193-195.
[4]铁富萍,马利青,王戈平,等.海晏县牦牛群中牛梨形虫病的血清学调查[J].青海畜牧兽医杂志,2011,41(4):30-31.
[5]秦思源,初冬,吴长江,等.甘肃省玛曲牦牛双芽巴贝斯虫血清学调查及风险因素分析[J].中国动物传染病学报,2017,25(4):56-59.
[6]刘凤鸣.宁安县马驽梨形虫病流行病学调查及防治[J].黑龙江畜牧兽医,1990(10):25-26.
[7]耿亚娜.延边地区马驽梨形虫病的分子流行病学调查与进化分析[D].延边:延边大学,2016.
[8]图尔,萨迪尔.新疆部分地区马属动物常见寄生虫病的流行病学调查[D].乌鲁木齐:新疆农业大学,2016.
[9]黄涛华.云南省部分地区宿主动物巴贝虫感染状况的调查研究[D].大理:大理大学,2016.
[10]朱玉涛,恰布旦·阿仔拜,宋瑞其,等.阿勒泰富蕴县放牧马驽巴贝斯虫和马泰勒虫抗体的检测初报[J].畜牧与兽医,2016,48(1):111-113.
[11]李永畅,恩克博力德,哈西巴特,等.南北疆部分地区马驽巴贝斯虫病血清学调查[J].中国兽医杂志,2016,52(2):6-8.
[12]DAVITKOV D,VUCICEVIC M,STEVANOVIC J,et al.Molecular detection and prevalence of Theileria equi and Babesia caballi in horses of central Balkan.[J].Acta parasitologica,2016,61(2):337.
[13]杨吉明,李德林.加强红河州边境地区动物疫病防控的建议[J].中国畜牧兽医文摘,2015(1):16-17.