钩吻生物碱急性毒性研究
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摘要
为评价钩吻生物碱的安全性,采用改良寇氏法考察钩吻生物碱的急性毒性。给小鼠一次性灌胃给予200、158、124.88、100 mg/kg不同剂量钩吻生物碱,连续观察7 d,并测定存活小鼠的血常规与脏器指数。结果表明,小鼠灌胃给药的最大耐受量为100 mg/kg,其LD50为136.60 mg/kg,LD50的95%可信限为126.74-147.23 mg/kg。与空白对照组相比,124.88、100 mg/kg剂量组小鼠血常规中的白细胞总数减少,血红蛋白减少,其他指标无显著性差异,脾脏指数下降,其他脏器指数无显著性差异。该结果为钩吻生物碱的安全性评价奠定了基础。
In order to evaluate the safety of Gelsemium elegans Benth. alkaloids, modified Korbor's method was used to study its acute toxicity.Different doses of Gelsemium elegans Benth. alkaloids were given to mice by intragastric administration,respectively, and observation was carried on for 7 days continuously. Mice routine blood parameter and viscera index were measured after 7 days. The results indicated that the maximal tolerance dose, LD50 and its 95% confidence limit of Gelsemium elegans Benth. alkaloids by intragastric administration were 100, 136.60, 126.74-147.23 mg/kg, respectively.There was no obvious change among 100, 124.88 mg/kg dose groups and control groups on routine blood parameter and viscera index except total white blood cells, hemoglobin and spleen index. It laid an experimental foundation for safety evaluation of Gelsemium elegans Benth. alkaloids.
In order to evaluate the safety of Gelsemium elegans Benth. alkaloids, modified Korbor's method was used to study its acute toxicity.Different doses of Gelsemium elegans Benth. alkaloids were given to mice by intragastric administration,respectively, and observation was carried on for 7 days continuously. Mice routine blood parameter and viscera index were measured after 7 days. The results indicated that the maximal tolerance dose, LD50 and its 95% confidence limit of Gelsemium elegans Benth. alkaloids by intragastric administration were 100, 136.60, 126.74-147.23 mg/kg, respectively.There was no obvious change among 100, 124.88 mg/kg dose groups and control groups on routine blood parameter and viscera index except total white blood cells, hemoglobin and spleen index. It laid an experimental foundation for safety evaluation of Gelsemium elegans Benth. alkaloids.
引文
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[2]农业部兽药评审中心.兽药研究技术指导原则汇编[M].北京:化学工业出版社,2012:84-87.
[3]刘妍,于洪婕.钩吻中毒的临床表现、发病机制及诊疗进展[J].医药前沿,2013,14:15-16.
[4]张雄,袁慧,文利新.钩吻干粉对肉鸡增重的试验研究[J].湖南农业大学学报:自然科学版,2003,29:70-71.
[5]刘道荣,黄泰康,王友顺,等.人参喂猪试验研究[J].畜牧与兽医,1994,26(2):67-68.
[6]陈翼胜.中国有毒植物[M].北京:科学出版社,1990:371-376.
[7]陈锐.断肠草中毒致呼吸骤停的救治体会[J].中国实用医药,2011,6(19):195-196.
[8]袁伯俊,廖明阳,李波.药物毒理学实验方法与技术[M].北京:化学工业出版社,2007:223-224.