盐胁迫对红叶石楠红罗宾生理特征影响的实验研究
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摘要
在NaCl盐胁迫环境下,以50 mmol·L~(–1)为浓度梯度,NaCl浓度分别为0、50、100、150、200 mmol·L~(–1),研究红叶石楠‘红罗宾’叶片含水量、叶绿素含量、质膜相对透性、丙二醛(MDA)含量、可溶性蛋白含量、可溶性糖含量、过氧化物酶(POD)活性变化以及抗坏血酸过氧化物酶(APX)活性变化等生理特征。结果表明:叶片含水量、丙二醛含量、可溶性糖含量、抗坏血酸过氧化物酶活性均随NaCl浓度的升高先增大,后降低;叶绿素含量、可溶性蛋白含量随NaCl浓度的升高先降低后升高又降低;膜相对透性随NaCl浓度的升高持续增大,而过氧化物酶(POD)活性随NaCl浓度的升高则先升高后降低,超过一定浓度后又升高。根据结果显示,红叶石楠‘红罗宾’具有一定耐盐性,可在适当盐碱地地区的环境绿化建设中推广应用。
The effect of salt stress to the physiological characteristics of Photinia Red Robin was investigated when exposed to 0, 50, 100, 150 and 200 mmol·L~(–1) of Na Cl by determining leaf water content, chlorophyll content, membrane permeability, malondialdehyde(MDA) content, soluble protein content, soluble sugar content, and the changes of peroxidase(POD) activity and ascorbate peroxidase(APX) activity. It was found that with the increase of Na Cl concentration, leaf water content, MDA content, soluble sugar content, and APX activity increased first and then decreased. Chlorophyll content and soluble protein content both had a variation tendency from decrease to increase and then increase to decrease, while the change of POD activity had a reverse variation. Membrane permeability continued increasing, which illustrated that Photinia Red Robin had a certain tolerance for salt and could be applied in the garden construction in saline area.
The effect of salt stress to the physiological characteristics of Photinia Red Robin was investigated when exposed to 0, 50, 100, 150 and 200 mmol·L~(–1) of Na Cl by determining leaf water content, chlorophyll content, membrane permeability, malondialdehyde(MDA) content, soluble protein content, soluble sugar content, and the changes of peroxidase(POD) activity and ascorbate peroxidase(APX) activity. It was found that with the increase of Na Cl concentration, leaf water content, MDA content, soluble sugar content, and APX activity increased first and then decreased. Chlorophyll content and soluble protein content both had a variation tendency from decrease to increase and then increase to decrease, while the change of POD activity had a reverse variation. Membrane permeability continued increasing, which illustrated that Photinia Red Robin had a certain tolerance for salt and could be applied in the garden construction in saline area.
引文
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[2]王真真,王卫娜,徐国超,等.红叶石楠研究现状及发展前景[J].黑龙江农业科学,2011(6):150–152.
[3]吴丽君.红叶石楠推广应用现状及前景分析[J].福建林业科技,2009,36(2):145–161.
[4]杜建会,魏兴虎.园林红叶植物新贵—红叶石楠[J].安徽农业科学2009,37(11):5263–5265.
[5]申亚梅,童再康,马进,等.海水胁迫下2种观赏植物的生长与生理特性比较[J].浙江林学院学报,2009,26(4):490–497.
[6]赵秀娟,韩雅楠,蔡禄.盐胁迫对植物生理生化特性的影响[J].湖北农业科学,2015,50(19):3897–3899.
[7]刘志高,申亚梅,邵伟丽.盐胁迫对红叶石楠‘红罗宾’幼苗生长与光合生理的影响[J].福建林学院学报,2012,32(3):238–242.
[8]项锡娜,陈泰豪,吴月燕,等.盐胁迫对红叶石楠‘鲁班’生理生化特性及叶片显微结构的影响[J].植物生理学报,2014,50(7):917–924.
[9]马妮娜.石楠属3种植物耐Na Cl胁迫能力的研究[D].南京林业大学,2010.
[10]李合生,孙群,赵世杰,等.植物生理生化实验原理和技术[M].高等教育出版社.
[11]杨少辉,季静,王罡,等.盐胁迫对植物影响的研究进展[J].分子植物育种,2006,4(3):139–142.
[12]王东明,贾媛,崔继哲.盐胁迫对植物的影响及植物盐适应性研究进展[J].中国农学通报,2009,25(04):124–128.
[13]潘瑞炽,王小菁,李娘辉.植物生理学[M].高等教育出版社.
[14]杨兰芳,庞静,彭小兰,等.紫外分光光度法测定植物过氧化氢酶活性[J].现代农业科技,2009,20:364–366.
[15]孙云,江春柳,赖钟雄,等.茶树鲜叶抗坏血酸过氧化物酶活性的变化规律及测定方法[J].热带作物学报,2008,29(5):562–566.
[16]沈文飚,徐朗莱,叶茂炳,等.抗坏血酸过氧化物酶活性测定的探讨[J].植物生理学通报,1996,32(3),203–205.
[17]蒋选利,李振岐,康振生.过氧化物酶与植物抗病性研究进展[J].西北农林科技大学学报,2001,29(6):124–129.
[18]HEYSER J W,NABORS M W.Growth,water content andsoluble accumulation of two tobacco cell lines cultured onsodium chloride,dextran and polyethylene glycol[J].Plant Physiology,1981,68(6):1454–1459.
[19]WAN C,SHAO G,CHEN Y,et al.Relationship between salt tolerance and chemical quality of soybean under salt stress[J].Chinese Journal of Oil Crop Sciences,2002,24:67–72.
[20]ASADA K.The water cycle in chloroplasts:Scavenging of active oxygens and dissipation of excess photons[J].Annual Review of Plant Physiology and Plant Molecular Biology,1999,50:601–639.
[21]ULLAH H,CHEN J G,WANG S,et al.Role of a het-erotrimeric G protein in regulation of Arabidopsis seed germi-nation[J].Plant Physiology,2002,129(2):897–907.
[22]HUANG C,HE W,GUO J,CANG X,Su P,ZHANG L.Increased sensitivity to salt stress in anascorbate-deficient Arabidopsis mutant[J].Journal Experimental Botany 2005,56(422):3041–3049.
[23]王树凤,胡韵雪,孙海菁,等.盐胁迫对2种栎树苗期生长和根系生长发育的影响[J].生态学报,2014,34(4):1021–1029.