焦苍术中药炮制规范项目的测定研究
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摘要
为深入对焦苍术中药饮片炮制规范的研究,充分发挥中药饮片的药效,保证药品生产加工阶段的质量,选取了不同产地不同批次的炮制样品,使用显微鉴别技术可以观察到纤维、石细胞、菊糖、导管等苍术的特征结构,利用薄层色谱分析技术可发现供试品色谱与苍术对照药材、苍术素对照品色谱在G板的相同位置显示相同颜色的斑点,该结果说明10批供试品中都含有苍术中的特征成分苍术素,可定性鉴定供试品是苍术样品.同时依据中国药典的实验方法,对样品的水分、总灰分、苍术素的含量进行测定,最终得出焦苍术炮制品水分含量应不得超过17.0%,总灰分含量应不得超过14.0%,苍术素含量按干燥品计算含量应不得少于0.08%.
To further study the processing standard of Chinese medicine and give full play to the pesticide effect as well as to guarantee the quality of drug production and processing stage, different batches of coked rhizoma atractylodis collected from different regions were verified by microscopic identification. Typefeature structure such as fiber, stone cell, inulin, catheter could be observed. The results by thin layer chromatography analysis techniques showed that the same position in G plate between samples and standard substance could be saw and it indicated the tested samples were rhizoma atractylodis. Besides, based on the Chinese pharmacopoeia method, the moisture and total ash as well as atisine chloride atractydin content in the samples were also determined so as to provide some reference data for further developing the processing standard of Chinese medicine on coked rhizoma atractylodis. It showed that the final moisture content in coke rhizoma atractylodis should not exceed 17.0%, the total ash content should not exceed 14.0%, and the content of atisine chloride atractydin calculated by dry product should not be less than 0.08%.
To further study the processing standard of Chinese medicine and give full play to the pesticide effect as well as to guarantee the quality of drug production and processing stage, different batches of coked rhizoma atractylodis collected from different regions were verified by microscopic identification. Typefeature structure such as fiber, stone cell, inulin, catheter could be observed. The results by thin layer chromatography analysis techniques showed that the same position in G plate between samples and standard substance could be saw and it indicated the tested samples were rhizoma atractylodis. Besides, based on the Chinese pharmacopoeia method, the moisture and total ash as well as atisine chloride atractydin content in the samples were also determined so as to provide some reference data for further developing the processing standard of Chinese medicine on coked rhizoma atractylodis. It showed that the final moisture content in coke rhizoma atractylodis should not exceed 17.0%, the total ash content should not exceed 14.0%, and the content of atisine chloride atractydin calculated by dry product should not be less than 0.08%.
引文
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[3]邓哲,黄宏进,汪文杰.茅苍术五种典型类型的形态研究[J].北方园艺,2014,5(19):147-150.
[4]马玲,张海弢,杨世海.关苍术的研究现状[J].人参研究,2012,6(3):51-53.
[5]付梅红,朱东海,方婧.苍术的化学分子生药学和药理学研究进展[J].中国中药杂志,2009,34(20):2669—2672.
[6]伊秀芝,浦卓,王冰梅,等.中药苍术抗真菌作用的研究及临床观察[J].北华大学学报,2000,1(6):492—493.
[7]赵爱梅.苍术的药理作用研究[J].光明中医,2009,24(1):181-182.
[8]刘艳菊,肖波,季光琼.苍术炮制前后挥发油的急性毒性实验[J].中国医院药学杂志,2013,33(20):1670-1673.
[9]王金华,薛宝云,梁爱华.苍术有效成分β-桉叶醇对小鼠小肠推进功能的影响[J].中国药学杂志,2002,37(4):266—268.
[10]篮方宇,李意.苍术炮制方法的初步研究[J].广西中医学院学报,2000,16(1):62-63.
[11]罗雪伦.苍术炮制的实验研究[J].中国中医药科技,2002,9(5):291.
[12]杜庆山.中药苍术炮制方法及理论研究进展[J].中国药学警戒,2012,9(7):439—441.
[13]龙芳,李会军,李萍.新技术和新方法在中药性状与显微鉴别中的应用[J].中国药学杂志,2012,37(8):1076-1080.
[14]中华人民共和国药典委员会.中华人民共和国药典一部[M].北京:中国医药科技出版社,2015:229.
[15]中华人民共和国药典委员会.中华人民共和国药典四部[M].北京:中国医药科技出版社,2015:202.
[16]张磊,欧阳臻,赵明.茅苍术中苍术酮、茅术醇、β-桉叶醇和苍术素的同时含量测定及其聚类分析[J].中国中药杂志,2010,35(6):725-728.