近红外透反射光谱测定单粒稻种的蛋白质含量
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摘要
为探索NIR光谱技术在水稻种子蛋白质含量分析中的应用,本研究细致分析了单粒稻种在不同光谱采集方式下的近红外光谱(NIRS)特征,并利用离子束诱变育种得到的水稻9311突变体库的种子,建立准确性较好的单粒糙米和单粒稻种的蛋白质定量模型。结果表明,与漫反射光谱采集方式下的单粒糙米蛋白质模型相比,透反射和透射光谱采集方式下能得到相关性较好的糙米蛋白质模型,其中单粒糙米蛋白质最优定量模型的决定系数(R~2)为0.97,预测均方根误差(RMSEP)为0.27%。在单粒稻种中,由于种壳的反射作用,漫反射光谱采集方式下依然无法建立准确性高的蛋白质模型,透反射光谱采集方式下能够建立具有一定预测能力的蛋白质定量模型(RMSEP=0.81%),透射光谱采集方式下能够建立准确性高的蛋白质定量模型(R~2=0.96,RMSEP=0.24%)。本研究结果为无损快速分析单粒稻种提供了一种解决方法。
To explore application of NIR spectroscopy in analysis of single rice seed protein content, we compared the near-infrared(NIR) spectral characteristics of rice seeds under different NIR measurement modes, and then established quantification models with relative good accuracy for protein content in single rice kernels and rice grains by using the seeds from the ion-beam-induced indica rice cultivar 9311 mutant library. Our results revealed that, for the assessment of protein content in single brown-rice kernels, the quantitative models based on the transflectance and transmittance NIR measurements gave rise to better accuracy than that based on the diffuse-reflectance measurement, and the best PLS regression was obtained with external validation set having coefficient of determination R~2=0.97 and root mean square errors of prediction(RMSEP)=0.27%. For the assessment of protein content in single rice grains, it is still hard to establish one quantitative model under diffuse-reflectance measurement because of the effect of rice husk. The quantitative model based on the transflectance NIR spectral data yielded a relatively higher accurate result(RMSEP=0.81%), while the optimal result was obtained under the transmittance measurement resulting in R~2=0.96 and RMSEP=0.24%. This work may provide a practical solution for rapid screening of single rice seeds.
To explore application of NIR spectroscopy in analysis of single rice seed protein content, we compared the near-infrared(NIR) spectral characteristics of rice seeds under different NIR measurement modes, and then established quantification models with relative good accuracy for protein content in single rice kernels and rice grains by using the seeds from the ion-beam-induced indica rice cultivar 9311 mutant library. Our results revealed that, for the assessment of protein content in single brown-rice kernels, the quantitative models based on the transflectance and transmittance NIR measurements gave rise to better accuracy than that based on the diffuse-reflectance measurement, and the best PLS regression was obtained with external validation set having coefficient of determination R~2=0.97 and root mean square errors of prediction(RMSEP)=0.27%. For the assessment of protein content in single rice grains, it is still hard to establish one quantitative model under diffuse-reflectance measurement because of the effect of rice husk. The quantitative model based on the transflectance NIR spectral data yielded a relatively higher accurate result(RMSEP=0.81%), while the optimal result was obtained under the transmittance measurement resulting in R~2=0.96 and RMSEP=0.24%. This work may provide a practical solution for rapid screening of single rice seeds.
引文
[1] 彭波,彭宇,彭娟,孔冬艳,何璐璐,孙艳芳,黄雅琴,宋世枝.水稻种子主要营养物质合成及调控研究与展望[J].热带作物学报,2018,39(6):1241-1251
[2] Champagne E T,Bett K L,Vinyard B T,Webb B D,Mcclung A M,Barton F E,Lyon B G,Moldenhauer K A K,Linscombe S D,Kohlwey D.Effects of drying conditions,final moisture content,and degree of milling on rice flavor[J].Cereal Chemistry,1997,74(5):566-570
[3] Silvela L,Rodgers R,Barrera A,Alexander D E.Effect of selection intensity and population-size on percent oil in maize,Zea mays L.[J].Theoretical and Applied Genetics,1989,78(2):298-304
[4] 曲龙,卢夏茹,李阳生,韦双双,袁潜华,马启林.水稻辐射诱变突变体的种子蛋白质成分分析[J].核农学报,2015,29(2):209-214
[5] Kjeldahl J Z.A new method for the determination of nitrogen in organic matter[J].Zeitschrift fur Analytische Chemie,1883,22(1):366-382
[6] Serrano S,Rincón J,García-Olmo F.Cereal protein analysis via Dumas method:Standardization of a micro-method using the EuroVector Elemental Analyser[J].Journal of Cereal Science,2013,58(1):31-36
[7] Song L,Wang Q,Wang C Y,Lin Y Q,Yu D,Xu Z P,Huang Q,Wu Y J.Effect of γ-irradiation on rice seed vigor assessed by near-infrared spectroscopy[J].Journal of Stored Products Research,2015,62:46-51
[8] Liu J H,Song L,Huang Q.Rapid screening astaxanthin-hyperproducing Haematococcus pluvialis mutants through near infrared spectroscopy[J].Letters in Applied Microbiology,2016,62(2):185-191
[9] Ma Y H,He H Q,Wu J Z,Wang C Y,Chao K L,Huang Q.Huang,Assessment of polysaccharides from mycelia of genus Ganoderma by mid-infrared and near-infrared spectroscopy[J].Scientific Reports,2018,8(1):1-10
[10] Roberts C A,Workman J J,Reeves B.Near-infrared Spectroscopy in Agriculture[M].USA:American Society of Agronomy,2004
[11] Vuuren,J A,Meyer A J H,Claassens S.Potential use of near infrared reflectance monitoring in precision agriculture[J].Communications in Soil Science & Plant Analysis,2006,37(15/20):2171-2184
[12] Qu J H,Liu D,Cheng J H,Sun D W,Ma J,Pu X H,Zeng A.Applications of near-infrared spectroscopy in food safety evaluation and control:A review of recent research advances[J].Critical Reviews in Food Science & Nutrition,2015,55(13):1939-1954
[13] Teye E,Huang N,Afoakwa X.Review on the potential use of near infrared spectroscopy (NIRS) for the measurement of chemical residues in food[J].Meat Science,2013,65(1):661-668
[14] Hacisalihoglu G,Gustin J L,Louisma J,Armstrong P,Peter G F,Walker A R,Settles A M.Enhanced single seed trait predictions in soybean (Glycine max) and robust calibration model transfer with near-infrared reflectance spectroscopy[J].Journal of Agriculture and Food Chemistry,2016,64(5):1079-1086
[15] 沈林峰,沈掌泉.应用近红外光谱和偏最小二乘回归法预测玉米中淀粉含量[J].化学分析计量,2008,17(6):26-28
[16] Cai J H.Near-infrared spectrum detection of wheat gluten protein content based on a combined filtering method[J].Journal of Aoac International,2017,100(5):1565-1568
[17] Peiris K H S,Dowell F H.Determining weight and moisture properties of sound and fusarium damaged single wheat kernels by near infrared spectroscopy[J].Cereal Chemistry,2011,88(1):45-50
[18] Natsuga M,Nakamura A,Kawano S.A Study on high-speed sorting of single-kernel brown rice using near-infrared spectroscopy (Part 3):Improvement of the developed continuous high-speed single-kernel brown rice sorting machine and the verification of its accuracy[J].Journal of the Japanese Society of Agricultural Machinery,2003,65(3):107-113
[19] Kawamura S,Natsuga M,Itoh K.Determination of undried rough rice constituent content using near-infrared transmission spectroscopy[J].Transactions of the American Society of Agricultural Engineers,1999,42(3):813-818
[20] Oido N,Iida M,Umeda M.A combine-mounted NIR spectroscopy-based sensor for single Rice Kernel protein content measurement[C].Automation Technology for Off-Road Equipment Proceedings of the 2004 Conference.Kyoto:Automation Technology for Off-Road Equipment,2004
[21] Rittiron R,Saranwong S,Kawano S.Useful tips for constructing a near infrared-based quality sorting system for single brown-rice kernels[J].Journal of Near Infrared Spectroscopy,2004,12(1):133-139
[22] Miryeong S,Franklineii B,Annam M C,Elainet C.Near-infrared spectroscopy for determination of protein and amylose in rice flour through use of derivatives[J].Food Chemistry,2004,81(3):341-344
[23] Xie L H,Tang S Q,Chen N,Luo J,Jiao G A,Shao G N,Wei X J,Hu P S.Optimisation of near-infrared reflectance model in measuring protein and amylose content of rice flour[J].Food Chemistry,2014,142(2):92-100
[24] Bagchi T B,Sharma S,Chattopadhyay K.Development of NIRS models to predict protein and amylose content of brown rice and proximate compositions of rice bran[J].Food Chemistry,2015,191(15):21-27
[25] Rinnan A,Fvan den Berg S,Engelsen B.Review of the most common pre-processing techniques for near-infrared spectra[J].Trac-Trends in Analytical Chemistry,2009,28(10):1201-1222
[26] Garcia-Alvarez M,Huidobro J F,Hermida M,Rodriguez-Otero J L.Major components of honey analysis by near-infrared transflectance spectroscopy[J].Journal of Agricultural and Food Chemistry,2000,48(11):5154-5158
[27] Adedipe O E,Johanningsmeier S D,Truong V D,Yencho G C.Development and validation of a near-infrared spectroscopy method for the prediction of acrylamide content in french-fried potato[J].Journal of Agricultural & Food Chemistry,2016,64(8):1850-1860
[28] Zou X B,Zhao J W,Povey M J W,Holmes M,Mao H P.Variables selection methods in near-infrared spectroscopy[J].Analytica Chimica Acta,2010,667(1/2):14-32
[29] Workman J,Weyer L.Practical Guide to Interpretive Near-Infrared Spectroscopy[M].New York:CRC Press,2007
[30] Wu P,Siesler H.The assignment of overtone and combination bands in the near infrared spectrum of polyamide 11[J].Journal of Near Infrared Spectroscopy,1999,7(1):328-337
[31] Kawamura S,Natsuga M,Takekura K,Itoh K.Development of an automatic rice-quality inspection system[J].Computers & Electronics in Agriculture,2003,40(1/2/3):115-126
[2] Champagne E T,Bett K L,Vinyard B T,Webb B D,Mcclung A M,Barton F E,Lyon B G,Moldenhauer K A K,Linscombe S D,Kohlwey D.Effects of drying conditions,final moisture content,and degree of milling on rice flavor[J].Cereal Chemistry,1997,74(5):566-570
[3] Silvela L,Rodgers R,Barrera A,Alexander D E.Effect of selection intensity and population-size on percent oil in maize,Zea mays L.[J].Theoretical and Applied Genetics,1989,78(2):298-304
[4] 曲龙,卢夏茹,李阳生,韦双双,袁潜华,马启林.水稻辐射诱变突变体的种子蛋白质成分分析[J].核农学报,2015,29(2):209-214
[5] Kjeldahl J Z.A new method for the determination of nitrogen in organic matter[J].Zeitschrift fur Analytische Chemie,1883,22(1):366-382
[6] Serrano S,Rincón J,García-Olmo F.Cereal protein analysis via Dumas method:Standardization of a micro-method using the EuroVector Elemental Analyser[J].Journal of Cereal Science,2013,58(1):31-36
[7] Song L,Wang Q,Wang C Y,Lin Y Q,Yu D,Xu Z P,Huang Q,Wu Y J.Effect of γ-irradiation on rice seed vigor assessed by near-infrared spectroscopy[J].Journal of Stored Products Research,2015,62:46-51
[8] Liu J H,Song L,Huang Q.Rapid screening astaxanthin-hyperproducing Haematococcus pluvialis mutants through near infrared spectroscopy[J].Letters in Applied Microbiology,2016,62(2):185-191
[9] Ma Y H,He H Q,Wu J Z,Wang C Y,Chao K L,Huang Q.Huang,Assessment of polysaccharides from mycelia of genus Ganoderma by mid-infrared and near-infrared spectroscopy[J].Scientific Reports,2018,8(1):1-10
[10] Roberts C A,Workman J J,Reeves B.Near-infrared Spectroscopy in Agriculture[M].USA:American Society of Agronomy,2004
[11] Vuuren,J A,Meyer A J H,Claassens S.Potential use of near infrared reflectance monitoring in precision agriculture[J].Communications in Soil Science & Plant Analysis,2006,37(15/20):2171-2184
[12] Qu J H,Liu D,Cheng J H,Sun D W,Ma J,Pu X H,Zeng A.Applications of near-infrared spectroscopy in food safety evaluation and control:A review of recent research advances[J].Critical Reviews in Food Science & Nutrition,2015,55(13):1939-1954
[13] Teye E,Huang N,Afoakwa X.Review on the potential use of near infrared spectroscopy (NIRS) for the measurement of chemical residues in food[J].Meat Science,2013,65(1):661-668
[14] Hacisalihoglu G,Gustin J L,Louisma J,Armstrong P,Peter G F,Walker A R,Settles A M.Enhanced single seed trait predictions in soybean (Glycine max) and robust calibration model transfer with near-infrared reflectance spectroscopy[J].Journal of Agriculture and Food Chemistry,2016,64(5):1079-1086
[15] 沈林峰,沈掌泉.应用近红外光谱和偏最小二乘回归法预测玉米中淀粉含量[J].化学分析计量,2008,17(6):26-28
[16] Cai J H.Near-infrared spectrum detection of wheat gluten protein content based on a combined filtering method[J].Journal of Aoac International,2017,100(5):1565-1568
[17] Peiris K H S,Dowell F H.Determining weight and moisture properties of sound and fusarium damaged single wheat kernels by near infrared spectroscopy[J].Cereal Chemistry,2011,88(1):45-50
[18] Natsuga M,Nakamura A,Kawano S.A Study on high-speed sorting of single-kernel brown rice using near-infrared spectroscopy (Part 3):Improvement of the developed continuous high-speed single-kernel brown rice sorting machine and the verification of its accuracy[J].Journal of the Japanese Society of Agricultural Machinery,2003,65(3):107-113
[19] Kawamura S,Natsuga M,Itoh K.Determination of undried rough rice constituent content using near-infrared transmission spectroscopy[J].Transactions of the American Society of Agricultural Engineers,1999,42(3):813-818
[20] Oido N,Iida M,Umeda M.A combine-mounted NIR spectroscopy-based sensor for single Rice Kernel protein content measurement[C].Automation Technology for Off-Road Equipment Proceedings of the 2004 Conference.Kyoto:Automation Technology for Off-Road Equipment,2004
[21] Rittiron R,Saranwong S,Kawano S.Useful tips for constructing a near infrared-based quality sorting system for single brown-rice kernels[J].Journal of Near Infrared Spectroscopy,2004,12(1):133-139
[22] Miryeong S,Franklineii B,Annam M C,Elainet C.Near-infrared spectroscopy for determination of protein and amylose in rice flour through use of derivatives[J].Food Chemistry,2004,81(3):341-344
[23] Xie L H,Tang S Q,Chen N,Luo J,Jiao G A,Shao G N,Wei X J,Hu P S.Optimisation of near-infrared reflectance model in measuring protein and amylose content of rice flour[J].Food Chemistry,2014,142(2):92-100
[24] Bagchi T B,Sharma S,Chattopadhyay K.Development of NIRS models to predict protein and amylose content of brown rice and proximate compositions of rice bran[J].Food Chemistry,2015,191(15):21-27
[25] Rinnan A,Fvan den Berg S,Engelsen B.Review of the most common pre-processing techniques for near-infrared spectra[J].Trac-Trends in Analytical Chemistry,2009,28(10):1201-1222
[26] Garcia-Alvarez M,Huidobro J F,Hermida M,Rodriguez-Otero J L.Major components of honey analysis by near-infrared transflectance spectroscopy[J].Journal of Agricultural and Food Chemistry,2000,48(11):5154-5158
[27] Adedipe O E,Johanningsmeier S D,Truong V D,Yencho G C.Development and validation of a near-infrared spectroscopy method for the prediction of acrylamide content in french-fried potato[J].Journal of Agricultural & Food Chemistry,2016,64(8):1850-1860
[28] Zou X B,Zhao J W,Povey M J W,Holmes M,Mao H P.Variables selection methods in near-infrared spectroscopy[J].Analytica Chimica Acta,2010,667(1/2):14-32
[29] Workman J,Weyer L.Practical Guide to Interpretive Near-Infrared Spectroscopy[M].New York:CRC Press,2007
[30] Wu P,Siesler H.The assignment of overtone and combination bands in the near infrared spectrum of polyamide 11[J].Journal of Near Infrared Spectroscopy,1999,7(1):328-337
[31] Kawamura S,Natsuga M,Takekura K,Itoh K.Development of an automatic rice-quality inspection system[J].Computers & Electronics in Agriculture,2003,40(1/2/3):115-126