MCCBA-Cu_2O-(MOF-5)三元复合材料抗菌性能的研究
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摘要
以微晶纤维素水凝胶(MCCBA)为载体、采用Cu_2O和MOF-5原位沉积制备MCCBA-Cu_2O-(MOF-5)三元复合材料。通过傅里叶红外光谱仪(FT-IR)、X射线衍射仪(XRD)、扫描电镜(SEM)和革兰氏阴性大肠杆菌(E.Coli)分别表征其结构和抗菌能力。研究结果表明,Cu_2O和MOF-5能够很好地沉积在MCCBA上,从而制备出MCCBA-Cu_2O-(MOF-5)三元复合材料;抗菌实验表明,在培养24 h后,细菌生长曲线呈现递减性变化,抑菌圈直径呈递增性变化。这说明单一MC-CBA并不具有抗菌性,经纳米Cu_2O复合改性的MCCBA表现出一定的抗菌性。在引入MOF-5形成三元复合材料的细菌浓度(OD值)为1.0,抑菌圈直径变为10 mm,可以证明制备的三元复合材料对革兰氏阴性大肠杆菌呈现出优异的抗菌能力。
In this work,MCC hydrogel-Cu_2O-(MOF-5)compsite was prepared by in-situ deposition of Cu_2O and MOF-5 onto the substrate of MCC hydrogel.The chemical structure and antibacterial property of the composite was characterized by FTIR,XRD,SEM and Escherichia Coli.The chemical structure characterization results displayed that Cu_2O and MOF-5 were deposited well on the surface of MCC hydrogel by in-situ method,thus prepared the MCC hydrogel-Cu_2O-(MOF-5)composite complex material.In addition,the antibacterial tests after 24 h cultivation,the OD curve of E coli bacterial growth showed decrease progressively,and the diameter of inhibition zone displayed incremental changes,indicating that pre-synthesized MCC hydrogel-Cu_2O-(MOF-5)composite material behaved the antibacterial property against Escherichia Coli.(E.Coli),the OD value changed to 1.0 and the diameter of inhibition zone increased up to 10 mm,due to introducing Nano Cu_2O and MOF-5 to composite of MCC hydrogel.
In this work,MCC hydrogel-Cu_2O-(MOF-5)compsite was prepared by in-situ deposition of Cu_2O and MOF-5 onto the substrate of MCC hydrogel.The chemical structure and antibacterial property of the composite was characterized by FTIR,XRD,SEM and Escherichia Coli.The chemical structure characterization results displayed that Cu_2O and MOF-5 were deposited well on the surface of MCC hydrogel by in-situ method,thus prepared the MCC hydrogel-Cu_2O-(MOF-5)composite complex material.In addition,the antibacterial tests after 24 h cultivation,the OD curve of E coli bacterial growth showed decrease progressively,and the diameter of inhibition zone displayed incremental changes,indicating that pre-synthesized MCC hydrogel-Cu_2O-(MOF-5)composite material behaved the antibacterial property against Escherichia Coli.(E.Coli),the OD value changed to 1.0 and the diameter of inhibition zone increased up to 10 mm,due to introducing Nano Cu_2O and MOF-5 to composite of MCC hydrogel.
引文
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[2]Mo Zunli,Hu Rere,Wang Yawen,et al.Review of Antibacterial Materials and Their Mechanisms[J].Materials Review,2014,28(1):50.莫尊理,胡惹惹,王雅雯,等.抗菌材料及其抗菌机理[J].材料导报,2014,28(1):50.
[3]ZHANG Xiang-rong,ZHANG Mei-yun,LI Jin-bao,et al.High Value-added Utilization of Cellulose:Microcrystalline Cellulose Preparation[J].China Pulp&Paper,2014,33(5):24.张向荣,张美云,李金宝,等.纤维素高值化利用制备微晶纤维素[J].中国造纸,2014,33(5):24.
[4]REN Jun-li,PENG Xin-wei,SUN Run-cang,et al.Progress in Functional Materials-Hydrogel Based on Hemicellulose[J].Transactions of China Pulp and Paper,2011,26(4):49.任俊莉,彭新文,孙润仓,等.半纤维素功能材料——水凝胶[J].中国造纸学报,2011,26(4):49.
[5]LIN Chun-xiang,ZHAN Huai-yu,LIU Ming-hua,et al.Homogeneous Graft Copolymerization of Cellulose Using an Ionic Liquid as a Reaction Medium[J].China Pulp&Paper,2009,28(2):32.林春香,詹怀宇,刘明华,等.纤维素在离子液体中的均相接枝共聚[J].中国造纸,2009,28(2):32.
[6]Hashemi D A.Chitosan/polyethylene glycol fumarate blend film:Physical and antibacterial properties[J].Carbohydrate Polymers,2013,92(1):48.
[7]Liu Ruilai,Cao Shengguang,Rao Ruihua,et al.Fabrication of poly[styrene-co-(maleic sodium anhydride)]/cellulose composite ultrafine hydrogel fiber and study on its drug release property[J].New Chemical Materials,2016(10):200.刘瑞来,曹胜光,饶瑞晔,等.聚苯乙烯-马来酸钠/纤维素复合纤维水凝胶的制备及其药物释放研究[J].化工新型材料,2016(10):200.
[8]Lin Y K,Chiang Y J,Hsu Y J.Metal-Cu2O core-shell nanocrystals for gas sensing applications:Effect of metal composition[J].Sensors&Actuators B Chemical,2014,204:190.
[9]Pang H,Gao F,Lu Q.Morphology effect on antibacterial activity of cuprous oxide[J].Chemical Communications,2009,9(9):1076.
[10]Jia R,Wang W Z,Sun S M,et,al.Crystallography Facet-Dependent Antibacterial Activity:The Case of Cu2O[J].Industrial&Engineering Chemistry Research,2011,50(50):10366.
[11]Horcajada P,Gref R,Baati T,et al.Metal-organic frameworks in biomedicine[J].Chemical Reviews,2012,112(2):1232.
[12]YANG Qiang,ZHANG Mei-yun,SONG Shun-xi.Metal Organic Frameworks Compounds and their Potential Application in Specialty Paper[J].China Pulp&Paper,2016,35(7):64.杨强,张美云,宋顺喜.金属有机骨架化合物及其在特种纸中的潜在应用[J].中国造纸,2016,35(7):64.
[13]Tamames-Tabar C,Imbuluzqueta E,Guillou N,et al.A Znazelate MOF:combining antibacterial effect[J].Crystengcomm,2014,17(2):456.
[14]Reddy N S,Shankara B S,Krishana P M,et al.Synthesis,Ch ARacterization,and Antibacterial Activity of Co(II),Ni(II),Cu(II),Zn(II),Cd(II),and Hg(II)Complexes of Schiff's Base Type Ligands Containing Benzofuran Moiety[J].International Journal of Inorganic Chemistry,2013,2013(5).
[15]Tan Caideng,Zhu Meijuan,Du Shuxia.Study on the Inhibition Zone Method in Antimicrobial Test[J].The Food Industry,2016(11):122.谭才邓,朱美娟,杜淑霞,等.抑菌试验中抑菌圈法的比较研究[J].食品工业,2016(11):122.
[16]Qiang D,Zhang M,Li J,et al.Selective hydrolysis of cellulose for the preparation of microcrystalline cellulose by phosphotungstic acid[J].Cellulose,2016,23(2):1199.
[17]Prabhakaran G,Murugan R.Synthesis of Cu2O Nanospheres and Cubes:Their Structural,Optical and Magnetic Properties[J].Advanced Materials Research,2014,938:114.
[18]Su X,Liao Q,Liu L,et al.Cu2O nanop ARticle-functionalized cellulose-based aerogel as high-performance visible-light photocatalyst[J].Cellulose,2017,24(2):1017.
[19]Yang Q,Zhang M,Song S,et al.Surface modification of PCC filled cellulose paper by MOF-5(Zn3(BDC)2)metal-organic frameworks for use as soft gas adsorption composite materials[J].Cellulose,2017:1.
[20]Jahan M S,Saeed A,He Z,et al.Jute as raw material for the prepARation of microcrystalline cellulose[J].Cellulose,2011,18(2):451.
[21]He L,Liu Y,Liu J,et al.Core-shell noble-metal@metal-organicframework nanoparticles with highly selective sensing property[J].Angewandte Chemie,2013,52(13):3741.
[22]Gabrielii I,Gatenholm P,Glasser W G,et al.Separation,characterization and hydrogel-formation of hemicellulose from aspen wood[J].Carbohydrate Polymers,2000,43(4):367.
[23]Xu J,Liu S,Liu Y.Co3O4/Zn O nanoheterostructure derived from core-shell ZIF-8@ZIF-67 for supercapacitors[J].Rsc Advances,2016,6(57):52137.
[24]Wu Peng,Liu Zhiming.Preparation and application of sodium alginate/cellulose hydrogel beads[J].Journal of Functional Materials,2015,46(10):10144.吴鹏,刘志明.海藻酸钠/纤维素水凝胶球的制备与应用[J].功能材料,2015,46(10):10144.