百会、大椎、足三里对癫痫大鼠神经保护机制的影响研究
|
摘要
目的:研究百会、大椎、足三里对不同机能大鼠海马组织中细胞因子白细胞介素(IL)-1β、IL-2、IL-4、IL-6、IL-10、IL-17、IL-18的表达差异,探讨该针刺方法对海马组织神经保护机制的影响。方法:按随机数字表法将60只雄性SD大鼠平均分为6组:正常空白组、正常经穴组、正常对照组、模型空白组、模型经穴组、模型对照组,每组10只。模型组均采用腹腔注射氯化锂-匹鲁卡品癫痫模型,正常组腹腔注射等量的生理盐水。每组大鼠治疗期间每日固定1次,每次30 min。正常空白组和模型空白组不予针刺只行固定;正常经穴组和模型经穴组,针刺处方为:百会、大椎、足三里;正常对照组和模型对照组,针刺处方为环跳、外关、手三里。治疗10 d后均处死取脑。结果:(1)与正常空白组比较,正常经穴组和正常对照组各细胞因子的指标虽略有差异,但无统计学意义(P>0.05);模型空白组IL-6、IL-18的表达显著上升(P<0.05),IL-1β、IL-2、IL-4、IL-10、IL-17表达无明显差异(P>0.05)。(2)与模型空白组比较,模型经穴组IL-10、IL-18的表达量下降(P<0.05),IL-1β、IL-2、IL-4、IL-6、IL-17的表达差异无统计学意义(P>0.05);模型对照组各组细胞因子的表达无明显差异(P>0.05)。(3)与正常经穴组比较,模型经穴组IL-1β、IL-2、IL-6、IL-18表达量上升(P<0.05), IL-4、IL-10、IL-17的表达无明显差异(P>0.05)。结论:(1)百会、大椎、足三里可能是通过调控癫痫大鼠体内IL-10和IL-18的表达,对癫痫疾病神经机制起到保护作用。(2)百会、大椎、足三里可能是通过影响IL-1β、IL-2、IL-6、IL-18的表达,对不同机能大鼠发挥其双向调节作用。
Objective:To study the differences of expressions of cytokines IL-1β, IL-2, IL-4, IL-6, IL-10, IL-17 and IL-18 in hippocampus of rats with different functions in Baihui(DU20), Dazhui(DU14) and Zusanli(ST36). To explore the effect of this acupuncture method on the neuroprotective mechanism of hippocampus. Method: Sixty male SD rats were randomly divided into 6 groups according to the random number table method: normal blank group, normal acupoint group, normal control group, model blank group, model acupoint group, model control group, 10 in each group. The model group received an intraperitoneal injection of a lithium chloride-pilocarpine epilepsy model. The normal group received intraperitoneal injection of the same volume of physiological saline. Each group's rats were fixed once a day for 30 minutes. The normal blank group and the model blank group did not need acupuncture and only need to be fixed. The acupuncture prescription of normal acupoint group and model acupoint group: Baihui(DU20), Dazhui(DU14) and Zusanli(ST36). In the normal control group and the model control group, the acupuncture prescriptions were Huantiao(GB30), Waiguan(SJ5), and Shousanli(LI10). After 10 days of treatment, the brains were sacrificed. Result:(1) Compared with the normal blank group, there were slight differences in the cytokines of the normal acupoint group and the normal control group, but there was no statistical significance(P>0.05). The expressions of IL-6 and IL-18 in the model blank group were significant. There was no significant difference in the expressions of IL-1β, IL-2, IL-4, IL-10 and IL-17(P>0.05).(2)Compared with the model blank group, the expressions of IL-10 and IL-18 decreasedin the model acupoint group(P<0.05). There was no significant difference in the expressions of IL-1β, IL-2, IL-4, IL-6 and IL-17(P>0.05). The expression of cytokines in the model control group had no significant difference(P>0.05).(3)Compared with the normal acupoint group, the expressions of IL-1β, IL-2, IL-6 and IL-18 increased in the model acupoint group(P<0.05). There was no significant difference in the expressions of IL-4, IL-10 and IL-17(P>0.05).Conclusion:(1)Baihui(DU20), Dazhui(DU14) and Zusanli(ST36) may protect the neurological mechanisms of epilepsy by regulating the expressions of IL-10 and IL-18 in epileptic rats.(2)Baihui(DU20), Dazhui(DU14) and Zusanli(ST36) may exert their bidirectional effects on different functional rats by affecting the expressions of IL-1β, IL-2, IL-6 and IL-18.
Objective:To study the differences of expressions of cytokines IL-1β, IL-2, IL-4, IL-6, IL-10, IL-17 and IL-18 in hippocampus of rats with different functions in Baihui(DU20), Dazhui(DU14) and Zusanli(ST36). To explore the effect of this acupuncture method on the neuroprotective mechanism of hippocampus. Method: Sixty male SD rats were randomly divided into 6 groups according to the random number table method: normal blank group, normal acupoint group, normal control group, model blank group, model acupoint group, model control group, 10 in each group. The model group received an intraperitoneal injection of a lithium chloride-pilocarpine epilepsy model. The normal group received intraperitoneal injection of the same volume of physiological saline. Each group's rats were fixed once a day for 30 minutes. The normal blank group and the model blank group did not need acupuncture and only need to be fixed. The acupuncture prescription of normal acupoint group and model acupoint group: Baihui(DU20), Dazhui(DU14) and Zusanli(ST36). In the normal control group and the model control group, the acupuncture prescriptions were Huantiao(GB30), Waiguan(SJ5), and Shousanli(LI10). After 10 days of treatment, the brains were sacrificed. Result:(1) Compared with the normal blank group, there were slight differences in the cytokines of the normal acupoint group and the normal control group, but there was no statistical significance(P>0.05). The expressions of IL-6 and IL-18 in the model blank group were significant. There was no significant difference in the expressions of IL-1β, IL-2, IL-4, IL-10 and IL-17(P>0.05).(2)Compared with the model blank group, the expressions of IL-10 and IL-18 decreasedin the model acupoint group(P<0.05). There was no significant difference in the expressions of IL-1β, IL-2, IL-4, IL-6 and IL-17(P>0.05). The expression of cytokines in the model control group had no significant difference(P>0.05).(3)Compared with the normal acupoint group, the expressions of IL-1β, IL-2, IL-6 and IL-18 increased in the model acupoint group(P<0.05). There was no significant difference in the expressions of IL-4, IL-10 and IL-17(P>0.05).Conclusion:(1)Baihui(DU20), Dazhui(DU14) and Zusanli(ST36) may protect the neurological mechanisms of epilepsy by regulating the expressions of IL-10 and IL-18 in epileptic rats.(2)Baihui(DU20), Dazhui(DU14) and Zusanli(ST36) may exert their bidirectional effects on different functional rats by affecting the expressions of IL-1β, IL-2, IL-6 and IL-18.
引文
[1] 吴立群,邹小秋,李荣蓉,等.针灸治疗癫痫的作用机理研究概况[J].江苏中医药,2018,50(2):82-85.
[2] Racine RJ.Modification of seizure activity by electrical stimulation:areas[J].Electroen- cephalogr Clin Neurophysiol,1975,38(1) :1.
[3] 余曙光,郭义.实验针灸学[M].上海:上海科学技术出版社,2014:150.
[4] 文帅,梁日生,杨卫忠.神经保护治疗与癫痫[J].国际神经病学神经外科学杂志,2010,37(3):254-259.
[5] 张慧敏,刘益民,王玉.TGF-β1对匹罗卡品癫痫大鼠海马炎性细胞因子的影响[J].安徽医科大学学报,2013,48(11):1316-1320.
[6] 井晓荣,靳俊功,李焕发,等.癫痫发作间期血清中IL-1β和IL-6水平分析研究[J].立体定向和功能性神经外科杂志,2014,27(2):73-78.
[7] 牛廷献,罗晓红,史智勇,等.癫痫大鼠血清和脑组织IL-1β、IL-6和TNF-α水平变化[J].放射免疫学杂志,2005(4):256-259.
[8] 薛小卫,黄银兰,刘倩,等.针灸治疗癫痫病症机制研究概述[J].辽宁中医药大学学报,2018,20(1):123-125.
[9] 柳成刚,姜元辉,石栏.针刺百会、腰奇穴对癫痫大鼠血清IL-1β、IL-6表达及海马神经元凋亡因子的影响[J].针灸临床杂志,2016,32(6):78-81.
[10] 孙宏,吴海琴,张桂莲,等.电针刺激癫痫大鼠“大椎”“百会”后对海马小胶质细胞及神经保护作用的研究[J].中华中医药杂志,2015,30(10):3741-3743.
[11] 何清溪,马冰宇,苏欣,等.温针灸百会穴和神门穴对癫痫大鼠细胞凋亡因子P53的影响[J].针灸临床杂志,2014,30(5):63-65.
[12] 赵新春,邢效如. 癫痫发病机制和治疗的研究进展[J]. 中国民康医学,2015,27(18): 74-76.
[13] 潘利忠. 平痫冲剂对慢性癫痫大鼠脑内谷氨酸、γ -氨基丁酸含量的影响及多耐药基因表达的研究[D]. 南京:南京中医药大学, 2010.
[14] 彭涛. IL-18在神经免疫性疾病中的作用[J]. 国外医学·神经病学神经外科学分册,2004(2):153-156.
[15] 陈少宗.针刺双向调节机制与机体的自组织原理[J].医学与哲学,2017,38(1):81-83
[16] 许建阳,冯琼.针灸双向良性作用通过神经-内分泌-免疫网络系统实现的假说[J].贵阳中医学院学报,1997(3):6-8.
[17] 张燕华.经络-神经-内分泌—免疫网络[J].华西医学,1998(3):57-58.
[18] 李超,郭旗.细胞因子IL-1β、IL-2、IL-6、IL-8和TNF-α水平在癫痫患者外周血中的变化及其意义[J].解放军预防医学杂志,2018,36(3):375-377.
[19] 傅勇,李希芝,郭玉洁,等.癫痫发作患者近期IL-1β、TNF-α水平与脑电图异常的关系[J].山东医药,2017,57(33):90-92.
[20] 庄俊鸿,云天,王年臻,等.丙戊酸钠与拉莫三嗪联用对原发性癫痫患者外周血TNF-αIL-2的影响研究[J].河北医学,2018,24(6):1007-1012.
[21] 桂兰,康靖,吕丽英.癫痫患儿血清HMGB1、IL-2、INF-γ水平变化及其意义[J].山东医药,2017,57(11):60-62.
[2] Racine RJ.Modification of seizure activity by electrical stimulation:areas[J].Electroen- cephalogr Clin Neurophysiol,1975,38(1) :1.
[3] 余曙光,郭义.实验针灸学[M].上海:上海科学技术出版社,2014:150.
[4] 文帅,梁日生,杨卫忠.神经保护治疗与癫痫[J].国际神经病学神经外科学杂志,2010,37(3):254-259.
[5] 张慧敏,刘益民,王玉.TGF-β1对匹罗卡品癫痫大鼠海马炎性细胞因子的影响[J].安徽医科大学学报,2013,48(11):1316-1320.
[6] 井晓荣,靳俊功,李焕发,等.癫痫发作间期血清中IL-1β和IL-6水平分析研究[J].立体定向和功能性神经外科杂志,2014,27(2):73-78.
[7] 牛廷献,罗晓红,史智勇,等.癫痫大鼠血清和脑组织IL-1β、IL-6和TNF-α水平变化[J].放射免疫学杂志,2005(4):256-259.
[8] 薛小卫,黄银兰,刘倩,等.针灸治疗癫痫病症机制研究概述[J].辽宁中医药大学学报,2018,20(1):123-125.
[9] 柳成刚,姜元辉,石栏.针刺百会、腰奇穴对癫痫大鼠血清IL-1β、IL-6表达及海马神经元凋亡因子的影响[J].针灸临床杂志,2016,32(6):78-81.
[10] 孙宏,吴海琴,张桂莲,等.电针刺激癫痫大鼠“大椎”“百会”后对海马小胶质细胞及神经保护作用的研究[J].中华中医药杂志,2015,30(10):3741-3743.
[11] 何清溪,马冰宇,苏欣,等.温针灸百会穴和神门穴对癫痫大鼠细胞凋亡因子P53的影响[J].针灸临床杂志,2014,30(5):63-65.
[12] 赵新春,邢效如. 癫痫发病机制和治疗的研究进展[J]. 中国民康医学,2015,27(18): 74-76.
[13] 潘利忠. 平痫冲剂对慢性癫痫大鼠脑内谷氨酸、γ -氨基丁酸含量的影响及多耐药基因表达的研究[D]. 南京:南京中医药大学, 2010.
[14] 彭涛. IL-18在神经免疫性疾病中的作用[J]. 国外医学·神经病学神经外科学分册,2004(2):153-156.
[15] 陈少宗.针刺双向调节机制与机体的自组织原理[J].医学与哲学,2017,38(1):81-83
[16] 许建阳,冯琼.针灸双向良性作用通过神经-内分泌-免疫网络系统实现的假说[J].贵阳中医学院学报,1997(3):6-8.
[17] 张燕华.经络-神经-内分泌—免疫网络[J].华西医学,1998(3):57-58.
[18] 李超,郭旗.细胞因子IL-1β、IL-2、IL-6、IL-8和TNF-α水平在癫痫患者外周血中的变化及其意义[J].解放军预防医学杂志,2018,36(3):375-377.
[19] 傅勇,李希芝,郭玉洁,等.癫痫发作患者近期IL-1β、TNF-α水平与脑电图异常的关系[J].山东医药,2017,57(33):90-92.
[20] 庄俊鸿,云天,王年臻,等.丙戊酸钠与拉莫三嗪联用对原发性癫痫患者外周血TNF-αIL-2的影响研究[J].河北医学,2018,24(6):1007-1012.
[21] 桂兰,康靖,吕丽英.癫痫患儿血清HMGB1、IL-2、INF-γ水平变化及其意义[J].山东医药,2017,57(11):60-62.