超声波-甲醇法提取茶皂素工艺研究
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摘要
以茶粕为原料,采用超声波-甲醇法从茶粕中提取茶皂素.分别考察了甲醇浓度、料液比、提取温度、提取时间等因素对茶皂素的提取率和纯度的影响.单因素试验结果表明:甲醇浓度90%、料液比1∶5、提取温度30℃和提取时间30 min时为宜.通过L9(34)正交试验进一步考察发现:甲醇浓度对茶皂素的提取率和纯度影响较大,提取时间和料液比分别对提取率和纯度影响较小.从实际应用考虑,选取甲醇浓度90%,提取温度30℃,料液比1∶7及提取时间30 min为适宜的工艺条件,此时,茶皂素提取率和纯度分别为96.21%和59.3%,该研究为茶粕的应用提供了一种新的可行方法.
Taking tea seed meal as the raw material, the technology that tea saponin is extracted from tea seed meal by ultrasonic assisted methanol extraction was studied in the paper. Based on the extraction efficiency and the purity of tea saponin from tea seed meal, effects of methanol content, mass ratio of solid to liquid,extraction temperature and extraction time were systematically investigated. The results obtained from the s ingle-factor experiments show that the suitable condition was methanol content of 90 %, solid-to-liquid ratio of1∶5, extraction temperature of 30 °C and extraction time of 30 min. With the othorgofial experiment of L9(34), the influencing factors of the extraction of tea saponin were further inspected, and the results indicate that methanol concentration has great influence on the extraction efficiency and purity of tea saponin, while the ex traction time and mass ratio of solid to liquid has an inferior effect on the extraction efficiency and purity of tea saponin. In consideration of the practical application, the condition, such as methanol concentration of 90 %,extraction temperature of 30 °C, mass ratio of solid to liquid of 1∶7 and extraction time of 30 min, was used to extract and purify the tea saponin from the tea seed meal, under which the extraction efficiency and the purity of the tea saponin were 96.21 % and 59.3 %, respectively. Therefore, a feasible method referred in the paper was established to utilize the tea seed meal.
Taking tea seed meal as the raw material, the technology that tea saponin is extracted from tea seed meal by ultrasonic assisted methanol extraction was studied in the paper. Based on the extraction efficiency and the purity of tea saponin from tea seed meal, effects of methanol content, mass ratio of solid to liquid,extraction temperature and extraction time were systematically investigated. The results obtained from the s ingle-factor experiments show that the suitable condition was methanol content of 90 %, solid-to-liquid ratio of1∶5, extraction temperature of 30 °C and extraction time of 30 min. With the othorgofial experiment of L9(34), the influencing factors of the extraction of tea saponin were further inspected, and the results indicate that methanol concentration has great influence on the extraction efficiency and purity of tea saponin, while the ex traction time and mass ratio of solid to liquid has an inferior effect on the extraction efficiency and purity of tea saponin. In consideration of the practical application, the condition, such as methanol concentration of 90 %,extraction temperature of 30 °C, mass ratio of solid to liquid of 1∶7 and extraction time of 30 min, was used to extract and purify the tea saponin from the tea seed meal, under which the extraction efficiency and the purity of the tea saponin were 96.21 % and 59.3 %, respectively. Therefore, a feasible method referred in the paper was established to utilize the tea seed meal.
引文
[1]李敏,王承明.油茶籽粕中茶皂素的提取工艺研究[J].中国粮油学报,2011,26(5):38-41.
[2]朱兴一,林海敏,陈秀,等.闪式提取油茶枯饼中茶皂素的工艺优化[J].农业工程学报,2011(增刊1):402-406.
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[6]高泽波,叶铭,薛玲玲,等.超声波-乙醇法提取油茶饼粕中茶皂素的工艺研究[J].化学工程与装备,2018(2):41-45.
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[9]Wu H,Li C,Li Z,et al.Simultaneous extraction of oil and tea saponin from Camellia oleifera Abel.seeds under subcritical water conditions[J].Fuel Processing Technology,2018,174:88-94.
[10]Yan J,Wu Z,Zhao Y,et al.Separation of tea saponin by twostage foam fractionation[J].Separation&Purification Technology,2011,80(2):300-305.
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[12]唐鹏程,王文渊,朱龙军.茶粕中茶皂素提取分离工艺的研究[J].化工管理,2017(2):185-186.
[13]王晓琴.水酶法提取茶叶籽油及副产物茶皂素工艺研究[J].中国粮油学报,2011,26(11):76-78.
[14]李佳桥,余修亮,曾林晖,等.响应面试验优化超声波辅助提取莲房原花青素工艺[J].食品科学,2016,37(12):40-45.
[15]张辉,熊道陵,陈金洲,等.石油醚-甲醇提取茶油和茶皂素工艺研究[J].有色金属科学与工程,2016,7(6):35-42.
[16]吕晓玲,李肇奖.CO2超临界萃取油茶皂苷的研究[J].食品与发酵工业,2005,31(1):23-26.
[17]杨瑞金,倪双双,张文斌,等.水媒法提取食用油技术研究进展[J].农业工程学报,2016,32(9):308-314.
[18]李刚,耿响.基于近红外光谱的茶粕中茶皂素快速测定分析方法研究[J].安徽农业科学,2015(22):223-224.
[19]赵敬娟,杜先锋.油茶皂苷对照品制备及高效液相色谱定量法的研究[J].中国油脂,2009,34(4):68-72.
[20]李洋,熊道陵,张建平,等.响应面法优化微波-超声耦合双水相提取茶多酚工艺[J].江西理工大学学报,2018,39(4):52-59.
[21]罗小燕,谭厚初.超声波测距系统中抗干扰措施的研究[J].江西理工大学学报,2006,27(6):67-69.
[22]张超,郭于翔,朱易春,等.低强度超声波强化ABR抗冲击负荷的试验研究[J].江西理工大学学报,2016,37(3):1-6.
[23]王金刚,郭培全,王西奎,等.空化效应在有机废水处理中的应用研究[J].化学进展,2005,17(3):549-553.
[24]李祥,曹江绒,曹万新,等.响应面法优化茶皂素的超声波辅助提取工艺[J].中国油脂,2013,38(12):72-75.
[25]戚晓阳,张颂培.超声-水浸提茶皂素及茶皂素水溶液应用研究[J].食品科学技术学报,2014,32(1):59-64.
[26]孙宾宾,杨博.高吸水性树脂超声波法制备研究进展及趋势[J].化学工程师,2014,28(4):30-32.
[27]陆顺忠,吴建文,杨素华,等.茶皂素提取工艺设计[J].农产品加工,2015(16):19-21.
[2]朱兴一,林海敏,陈秀,等.闪式提取油茶枯饼中茶皂素的工艺优化[J].农业工程学报,2011(增刊1):402-406.
[3]高凯翔,李秋庭,陆顺忠,等.茶皂素的提取工艺研究[J].粮油食品科技,2010,18(5):22-25.
[4]熊道陵,张团结,陈金洲,等.茶皂素提取及应用研究进展[J].化工进展,2015(4):1080-1087.
[5]丁富成,鲍妮娜,胡巧缘.茶皂素的提取方法研究进展[J].阴山学刊(自然科学版),2018,32(1):116-118.
[6]高泽波,叶铭,薛玲玲,等.超声波-乙醇法提取油茶饼粕中茶皂素的工艺研究[J].化学工程与装备,2018(2):41-45.
[7]Ahmed H O A,Wang C.Determination of tea saponin in camellia seed oil with UV and HPLC analysis[J].World Journal of Engineering&Technology,2015,3(4):30-37.
[8]Wu Y Q,Man W D.Study on ultrasonic assisted-precipitation method combined purification of tea saponin[J].Advanced Materials Research,2013,634-638:1552-1556.
[9]Wu H,Li C,Li Z,et al.Simultaneous extraction of oil and tea saponin from Camellia oleifera Abel.seeds under subcritical water conditions[J].Fuel Processing Technology,2018,174:88-94.
[10]Yan J,Wu Z,Zhao Y,et al.Separation of tea saponin by twostage foam fractionation[J].Separation&Purification Technology,2011,80(2):300-305.
[11]吴雪辉,张喜梅.茶皂素微波提取过程的优化及数学描述[J].华南理工大学学报(自然科学版),2009,37(4):125-129.
[12]唐鹏程,王文渊,朱龙军.茶粕中茶皂素提取分离工艺的研究[J].化工管理,2017(2):185-186.
[13]王晓琴.水酶法提取茶叶籽油及副产物茶皂素工艺研究[J].中国粮油学报,2011,26(11):76-78.
[14]李佳桥,余修亮,曾林晖,等.响应面试验优化超声波辅助提取莲房原花青素工艺[J].食品科学,2016,37(12):40-45.
[15]张辉,熊道陵,陈金洲,等.石油醚-甲醇提取茶油和茶皂素工艺研究[J].有色金属科学与工程,2016,7(6):35-42.
[16]吕晓玲,李肇奖.CO2超临界萃取油茶皂苷的研究[J].食品与发酵工业,2005,31(1):23-26.
[17]杨瑞金,倪双双,张文斌,等.水媒法提取食用油技术研究进展[J].农业工程学报,2016,32(9):308-314.
[18]李刚,耿响.基于近红外光谱的茶粕中茶皂素快速测定分析方法研究[J].安徽农业科学,2015(22):223-224.
[19]赵敬娟,杜先锋.油茶皂苷对照品制备及高效液相色谱定量法的研究[J].中国油脂,2009,34(4):68-72.
[20]李洋,熊道陵,张建平,等.响应面法优化微波-超声耦合双水相提取茶多酚工艺[J].江西理工大学学报,2018,39(4):52-59.
[21]罗小燕,谭厚初.超声波测距系统中抗干扰措施的研究[J].江西理工大学学报,2006,27(6):67-69.
[22]张超,郭于翔,朱易春,等.低强度超声波强化ABR抗冲击负荷的试验研究[J].江西理工大学学报,2016,37(3):1-6.
[23]王金刚,郭培全,王西奎,等.空化效应在有机废水处理中的应用研究[J].化学进展,2005,17(3):549-553.
[24]李祥,曹江绒,曹万新,等.响应面法优化茶皂素的超声波辅助提取工艺[J].中国油脂,2013,38(12):72-75.
[25]戚晓阳,张颂培.超声-水浸提茶皂素及茶皂素水溶液应用研究[J].食品科学技术学报,2014,32(1):59-64.
[26]孙宾宾,杨博.高吸水性树脂超声波法制备研究进展及趋势[J].化学工程师,2014,28(4):30-32.
[27]陆顺忠,吴建文,杨素华,等.茶皂素提取工艺设计[J].农产品加工,2015(16):19-21.