干预Bcl-2基因表达对骨肉瘤细胞侵袭、迁移能力的影响
摘要
目的探究干扰B细胞淋巴瘤/白血病(Bcl)-2基因表达对骨肉瘤细胞侵袭、迁移的影响及可能的作用机制。方法采用siRNA特异性下调Bcl-2基因表达,实验分为空白组、MG-63-NC组(转染siRNA-NC)和MG-63-siRNA组(转染siRNA-Bcl-2)。Transwell法检测细胞迁移、侵袭能力,Western印迹检测细胞中Bcl-2、β-连环蛋白(catenin)、c-Myc蛋白的表达量。结果 MG-63-siRNA组Bcl-2蛋白表达量显著低于空白组(P<0.05);MG-63-siRNA组细胞侵袭、迁移能力显著抑制(P<0.05);与空白组相比,MG-63-siRNA组细胞中β-catenin、c-Myc蛋白表达显著下调(P<0.05)。结论干扰Bcl-2抑制骨肉瘤MG-63细胞的迁移和侵袭,可能通过抑制Wnt/β-catenin信号通路介导的下游靶蛋白c-Myc的表达。
引文
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2 Haddox CL,Han G,Anijar L,et al.Osteosarcoma in pediatric patients and young adults:a single institution retrospective review of presentation,therapy,and outcome[J].Sarcoma,2014;2014(30):402509.
3 Goriˇcar K,Kovaˇc V,Jazbec J,et al.Influence of the folate pathway and transporter polymorphisms on methotrexate treatment outcome in osteosarcoma[J].Pharmacogenet Genomics,2014;24(10):514-21.
4 农峰,蓝常贡,唐毓金.骨肉瘤易感性与相关基因多态性的研究进展[J].现代肿瘤医学,2017;25(2):304-7.
5 封志强.Bcl-2在儿童骨肉瘤中的表达与细胞凋亡的相关性及临床意义[J].社区医学杂志,2013;11(4):26-30.
6 易生辉,秦刚,黄肖华,等.骨肉瘤的治疗进展[J].医学综述,2017;23(8):1529-32.
7 黎承军,缪叶佳,流小舟,等.骨肉瘤MG-63细胞中Survivin的表达及其反义寡核苷酸对细胞增殖与凋亡的作用[J].临床肿瘤学杂志,2014;19(1):14-7.
8 韦秋业,王迎彬,农峰,等.miRNA-9对骨肉瘤MG-63细胞增殖,凋亡的作用及其机制[J].现代肿瘤医学,2017;25(1):25-9.
9 Su Y,Wan D,Song W.Dryofragin inhibits the migration and invasion of human osteosarcoma U2OS cells by suppressing MMP-2/9 and elevatingTIMP-1/2 through PI3K/AKT and p38 MAPK signaling pathways[J].Anticancer Drugs,2016;27(7):660-8.
10 Zeng H,Zhang Z,Dai X,et al.Increased expression of microRNA-199b-5 p associates with poor prognosis through promoting cell proliferation,invasion and migration abilities of human osteosarcoma[J].Pathol Oncol Res,2016;22(2):253-60.
11 尹智勇,杨俊元,祁宏.Bcl-2蛋白质家族调控细胞凋亡机制的研究进展[J].信阳师范学院学报,2017;30(2):340-4.
12 张晓慧,赵建华,唐金海.Wnt/β-catenin信号通路在乳腺癌发生,转移及耐药形成中的作用[J].国际检验医学杂志,2015;36(16):2417-9.
13 冯晓灵,刘丹.白头翁皂苷D对人乳腺癌MCF-7细胞中Wnt/β-链蛋白信号通路的影响[J].东南大学学报:医学版,2017;36(3):379-83.
14 Su XY,Zou X,Chen QZ,et al.Follicle-Stimulating hormoneβ-subunit potentiates bone morphogenetic protein 9-induced osteogenic differentiation in mouse embryonic fibroblasts[J].J Cell Biochem,2017;118(7):1792-802.
15 Chen RJ,Wu PH,Ho CT,et al.P53-dependent downregulation of h TERT protein expression and telomerase activity induces senescence in lung cancer cells as a result of pterostilbene treatment[J].Cell Death Dis,2017;8(8):e2985.
16 Guo X,Gao H,Liu X,et al.Porous Li-containing biphasic calcium phosphate scaffolds fabricated by three-dimensional plotting for bone repair[J].Rsc Adv,2017;7(55):34508-16.