精子DNA碎片与年龄、精液常规参数和疾病因素的相关性分析
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摘要
目的分析男性患者精子DNA碎片率和年龄、精液常规参数及疾病因素之间的相关性。方法按照年龄将患者分为20岁～25岁,26岁～30岁,31岁～35岁,36岁～40岁,41岁～45岁及>45岁组。采用染色质结构分析(SCSA)实验检测精子DNA碎片率,按照世界卫生组织(WHO)标准检测精液各项参数。采用SPSS 19.0软件进行统计分析处理,分析精子DNA碎片率和年龄、精液常规参数及疾病因素之间的相关性。结果年龄与精子DNA碎片、精子活力、HDS之间存在负相关关系,与精子浓度之间的相关性无统计学意义。精子DNA碎片与精子浓度、精子活力之间存在负相关关系,与HDS之间存在正相关关系。精子HDS与精子浓度、精子活力之间存在负相关关系。DFI值的异常与弱精症、男性不育和尿路感染这3类男性疾病的关系较为密切。结论随年龄增长,精子活力可能会降低,DNA碎片率增加。因此精子DNA碎片检测可作为一项重要的精子质量评估指标,为全面、客观评估男性生育力提供了有效信息。
Objective To analyze the correlation of sperm DNA fragmentation, age, semen parameters and disease factors in male patients. Methods Patients were divided into six groups according to age, which were 20～25, 26～30, 31～35, 36～40, 41～45 and 45 above.Sperm DNA fragmentation was measured by chromatin structure analysis(SCSA) and semen parameters were tested according to World Health Organization(WHO) standards.Statistical analysis was performed using SPSS 19.0 software to analyze the correlation of sperm DNA fragmentation, age, semen parameters, and disease factors. Results There was a negative correlation between age and sperm DNA fragmentation, sperm motility, and HDS, and there was no statistically significant correlation with sperm concentration. There was a negative correlation between sperm DNA fragments and sperm concentration and sperm motility, and there was a positive correlation between HDS and HDS. There was a negative correlation between sperm HDS and sperm concentration and sperm motility. The abnormalities in DFI were closely related to the three types of male diseases, such as weak spermatogenesis, male infertility and urinary tract infection. Conclusions As the age increases, sperm motility may decrease and DNA fragmentation rate increases. Therefore, sperm DNA fragmentation detection can be used as an important indicator of sperm quality assessment, providing effective information for comprehensive and objective assessment of male fertility.
Objective To analyze the correlation of sperm DNA fragmentation, age, semen parameters and disease factors in male patients. Methods Patients were divided into six groups according to age, which were 20～25, 26～30, 31～35, 36～40, 41～45 and 45 above.Sperm DNA fragmentation was measured by chromatin structure analysis(SCSA) and semen parameters were tested according to World Health Organization(WHO) standards.Statistical analysis was performed using SPSS 19.0 software to analyze the correlation of sperm DNA fragmentation, age, semen parameters, and disease factors. Results There was a negative correlation between age and sperm DNA fragmentation, sperm motility, and HDS, and there was no statistically significant correlation with sperm concentration. There was a negative correlation between sperm DNA fragments and sperm concentration and sperm motility, and there was a positive correlation between HDS and HDS. There was a negative correlation between sperm HDS and sperm concentration and sperm motility. The abnormalities in DFI were closely related to the three types of male diseases, such as weak spermatogenesis, male infertility and urinary tract infection. Conclusions As the age increases, sperm motility may decrease and DNA fragmentation rate increases. Therefore, sperm DNA fragmentation detection can be used as an important indicator of sperm quality assessment, providing effective information for comprehensive and objective assessment of male fertility.
引文
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[17] 陈庆,李向红,符生鱼,等.睡眠时间对精液常规参数、精子形态及精子DNA完整性的影响[J].实用预防医学,2017,24(6):650-652.
[18] Schulte RT,Ohl DM,Smith GD.Sperm DNA damage in male infertility:Etiologies,assays,and outcomes[J].J Assist Reprod Genet,2010,27(1):3-12.
[19] 杨晓玉,张燕,孙雪萍,等.精子染色质结构分析预测夫精宫腔内人工授精结局的临床研究[J].中华男科学杂志,2011,17(11):977-983.
[20] Anifandis G,Bounartzi T,Messini CI,et al.Sperm DNA fragmentation measured by Halosperm does not impact on embryo quality and ongoing pregnancy rates in IVF/ICSI treatments[J].Andrologia,2015,47(3):295-302.
[21] Vagnini L,Baruffi RL,Mauri AL,et al.The effects of male age on sperm DNA damage in an infertile population[J].Reproductive Biomedicine Online,2007,15(5):514-519.
[22] Winkle T,Rosenbusch B,Gagsteiger F,et al.The correlation between male age,sperm quality and sperm DNA fragmentation in 320 men attending a fertility center[J].Journal of Assisted Reproduction & Genetics,2009,26(1):41-46.
[23] 杨译,姜辉,张海娇,等.男性不育患者年龄与精子DNA碎片和精液常规参数的相关性分析[J].中国性科学,2012,21(2):17-19.
[24] Bungum M,Humaidan P,Axmon A,et al.Sperm DNA integrity assessment in prediction of assisted reproduction technology outcome[J].Human Reproduction,2007,22(1):174-179.
[25] 韩小克,苑辉,关立军,等.精子DNA碎片与精液各参数的相关性分析[J].中国计划生育和妇产科,2016,8(10):59-62.
[2] NallellaKP,Sharma RK,Aziz N,et al.Significance of sperm characteristics in the evaluation of male infertility[J].Fertility & Sterility,2006,85(3):629-634.
[3] Sikka SC,Hellstrom Wayne JG.Current updates on laboratory techniques for the diagnosis of male reproductive failure[J].Asian Journal of Andrology,2016,18(3):392-401.
[4] Lewis SEM,Agbaje I,Alvarez J.Sperm DNA tests as useful adjuncts to semen analysis[J].Systems Biology in Reproductive Medicine,2008,54(3):111-125.
[5] Aitken RJ,Koppers AJ.Apoptosis and DNA damage in human spermatozoa[J].Asian Journal of Andrology,2011,13(1):36-36.
[6] Karydis S,Asimakopoulos B,Papadopoulos N,et al.ICSI outcome is not associated with the incidence of spermatozoa with abnormal chromatin condensation[J].Vivo,2005,19(5):921-925.
[7] Barratt CL.Semen analysis is the cornerstone of investigation for male infertility[J].Practitioner,2007,251(1690):15-17.
[8] World Health Organization.WHO laboratory manual for the examination of human semen and semen-cervical mucus interactions[J].International Journal ofAndrology,2010,19(3):149-149.
[9] 姜辉,商学军,陈亮,等.男性生殖遗传学检查专家共识[J].中华男科学杂志,2015,21(12):1138-1142.
[10] Huang CC,Lin DP,Tsao HM,et al.Sperm DNA fragmentation negatively correlates with velocity and fertilization rates but might not affect pregnancy rates[J].Journal of Urology,2006,175(2):661-662.
[11] Mahfouz R,Sharma R,Thiyagarajan A,et al.Semen characteristics and sperm DNA fragmentation in infertile men with low and high levels of seminal reactive oxygen species[J].Fertility & Sterility,2010,94(6):2141-2146.
[12] Marcon LBG.Transient DNA strand breaks during mouse and human spermiogenesis new insights in stage specificity and link to chromatin remodeling[J].Biology of Reproduction,2004,70(4):910-918.
[13] Morris ID,Ilott S,Dixon L,et al.The spectrum of DNA damage in human sperm assessed by single cell gel electrophoresis (Comet assay) and its relationship to fertilization and embryo development[J].Human Reproduction,2002,17(4):990-998.
[14] Sakkas D,Alvarez JG.Sperm DNA fragmentation:mechanisms of origin,impact on reproductive outcome,and analysis[J].Fertility & Sterility,2010,93(4):1027-1036.
[15] 刘炜,付睿,赵晨,等.黄精赞育胶囊对弱精子症患者精子DNA完整性的影响[J].中国男科学杂志,2016,30(4):34-37.
[16] 阳方,李俊君,董良,等.精子DNA完整性损伤的发生机制及诊断治疗[J].中国男科学杂志,2016,30(9):70-72.
[17] 陈庆,李向红,符生鱼,等.睡眠时间对精液常规参数、精子形态及精子DNA完整性的影响[J].实用预防医学,2017,24(6):650-652.
[18] Schulte RT,Ohl DM,Smith GD.Sperm DNA damage in male infertility:Etiologies,assays,and outcomes[J].J Assist Reprod Genet,2010,27(1):3-12.
[19] 杨晓玉,张燕,孙雪萍,等.精子染色质结构分析预测夫精宫腔内人工授精结局的临床研究[J].中华男科学杂志,2011,17(11):977-983.
[20] Anifandis G,Bounartzi T,Messini CI,et al.Sperm DNA fragmentation measured by Halosperm does not impact on embryo quality and ongoing pregnancy rates in IVF/ICSI treatments[J].Andrologia,2015,47(3):295-302.
[21] Vagnini L,Baruffi RL,Mauri AL,et al.The effects of male age on sperm DNA damage in an infertile population[J].Reproductive Biomedicine Online,2007,15(5):514-519.
[22] Winkle T,Rosenbusch B,Gagsteiger F,et al.The correlation between male age,sperm quality and sperm DNA fragmentation in 320 men attending a fertility center[J].Journal of Assisted Reproduction & Genetics,2009,26(1):41-46.
[23] 杨译,姜辉,张海娇,等.男性不育患者年龄与精子DNA碎片和精液常规参数的相关性分析[J].中国性科学,2012,21(2):17-19.
[24] Bungum M,Humaidan P,Axmon A,et al.Sperm DNA integrity assessment in prediction of assisted reproduction technology outcome[J].Human Reproduction,2007,22(1):174-179.
[25] 韩小克,苑辉,关立军,等.精子DNA碎片与精液各参数的相关性分析[J].中国计划生育和妇产科,2016,8(10):59-62.