分子标记技术在黄瓜农艺性状基因定位上的应用
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摘要
分子标记是继形态学、细胞学和生化标记后的新型技术,随着分子生物学的迅猛发展,该技术已在蔬菜育种上广泛应用。本文概述了分子标记的概念及分类,包括典型标记技术的原理及优缺点,详述其近年在黄瓜(Cucumissativus)外观、品质、产量与性型、抗逆、种质资源鉴定以及品种纯度鉴定方面相关基因定位上的应用,最后对其在基因定位及辅助选择育种方面的应用前景进行了展望。
Molecular marker is a new technique after morphological, cytological and biochemical markers. With the rapid development of molecular biology, the technique has been widely used in vegetable breeding. This article summarizes the concept and classification of molecular markers, including the principle, advantages and disadvantages of typical marker techniques, and details of their application in recent years in the gene mapping of cucumber appearance, quality, yield and gynoecious, adversity resistance, germplasm resources identification as well as seed purity test. Finally, the application of molecular markers in gene mapping and assisted selection was prospected.
Molecular marker is a new technique after morphological, cytological and biochemical markers. With the rapid development of molecular biology, the technique has been widely used in vegetable breeding. This article summarizes the concept and classification of molecular markers, including the principle, advantages and disadvantages of typical marker techniques, and details of their application in recent years in the gene mapping of cucumber appearance, quality, yield and gynoecious, adversity resistance, germplasm resources identification as well as seed purity test. Finally, the application of molecular markers in gene mapping and assisted selection was prospected.
引文
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