荧光相关光谱法直接测定溶液中金纳米粒子浓度
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摘要
本文利用金纳米粒子(AuNPs)的荧光特性,采用荧光相关光谱建立了溶液中AuNPs摩尔浓度测定的新方法。实验优化了激光强度和采样时间等实验条件。在优化的条件下检测,微区内的粒子数与AuNPs浓度具有良好线性关系。该方法成功地用于合成的溶液中AuNPs摩尔浓度的测定,测定结果与传统的方法基本一致。该方法与现有方法相比,具有灵敏高、快速简便、样品和试剂用量少、样品无损等特点,不仅可以用于溶液中AuNPs浓度的测定,而且有望应用于活细胞内AuNPs的实时原位分析。
A method for determination of gold nanoparticles(AuNPs)in the solution was established with fluorescence correlation spectroscopy(FCS)based on the photoluminescence of AuNPs.By optimizing the laser intensity,sampling time etc.,agood linear relationship between the determined number of AuNPs in the detection region and the concentration of AuNPs was established.The method was successfully applied to measure the molar concentration of AuNPs in the prepared samples and the results determined by FCS almost agreed with that by the classical UV-Vis method.Compared with the present methods,the method is highly-sensitive,very quick,simple,convenient,of low requirement for sample and reagent,and nondestructive for samples,therefore demonstrating agreat application potential for in situ determination of AuNPs in living cells.
A method for determination of gold nanoparticles(AuNPs)in the solution was established with fluorescence correlation spectroscopy(FCS)based on the photoluminescence of AuNPs.By optimizing the laser intensity,sampling time etc.,agood linear relationship between the determined number of AuNPs in the detection region and the concentration of AuNPs was established.The method was successfully applied to measure the molar concentration of AuNPs in the prepared samples and the results determined by FCS almost agreed with that by the classical UV-Vis method.Compared with the present methods,the method is highly-sensitive,very quick,simple,convenient,of low requirement for sample and reagent,and nondestructive for samples,therefore demonstrating agreat application potential for in situ determination of AuNPs in living cells.
引文
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[3]Woznica E,Wojcik M M,Wojciechowski M,Mieczkowski J,Bulska E,Maksymiuk K,Michalska A.Anal Chem,2012,84(10):4437.
[4]TIAN R,XUE L H,MA C,WANG J Y,SUN X H,LUAN J M,LI X J.J Anal Sci(田锐,薛李慧,马晨,王靖原,孙雪花,栾建明,李晓江.分析科学学报),2016,32(3):361.
[5]Mintzer M A,Simanek E E.Chem Rev,2009,109(2):259.
[6]Xing R T,Liu K,Jiao T F,Zhang N,Ma K,Zhang R Y,Zou Q L,Ma G H,Yan X H.Adv Mater,2016,28(19):3669.
[7]Han G J,Xing Z,Dong Y H,Zhang S C,Zhang X R.Angew Chem Int Edit,2011,50(15):3462.
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[12]Xu X,Li T,Xu Z X,Wei H J,Lin R Y,Xia B,Liu F,Li N.Anal Chem,2015,87(5):2576.
[13]Hu X C,Su D,Du Z X,Huang X Y,Dong C Q,Ren J C.Microchim Acta,2016,183(8):2457.
[14]He H,Xie C,Ren J.Anal Chem,2008,80(15):5951.
[15]Wang J J,Huang X Y,Liu H,Dong C Q,Ren J C.Anal Chem,2017,89(10):5230.
[16]Storhoff J J,Elghanian R,Mucic R C,Mirkin C A,Letsinger R L.J Am Chem Soc,1998,120(9):1959.
[17]Kim S A,Heinze K G,Schwille P.Nat Methods,2007,4(11):963.