摘要
目的研究血清微小RNA-6861-5p(miR-6861-5p)联合糖类抗原153(CA153)对乳腺癌的诊断价值。方法选择2015年4月~2018年4月辽宁省鞍山市肿瘤医院(以下简称"我院")收治的乳腺癌患者83例为研究对象,作为乳腺癌组。另取同期于我院接受诊治的乳腺良性疾病患者80例作为乳腺良性疾病组,再取同期在我院进行体检的健康人者80名作为对照组。分别采用实时荧光定量聚合酶链反应检测各组人员血清miR-6861-5p表达情况,采用化学发光法检测血清CA153水平。比较三组血清miR-6861-5p与CA153水平,并分析上述两项指标与乳腺癌患者临床病理特征的关系。采用受试者工作特征(ROC)曲线分析血清miR-6861-5p与CA153水平联合检测对乳腺癌的诊断效能。结果乳腺癌组患者血清miR-6861-5p与CA153水平分别高于乳腺良性疾病组与对照组,差异有统计学意义(均P <0.05)。Ⅰ~Ⅱ期与无淋巴结转移的乳腺癌患者血清miR-6861-5p水平分别低于Ⅲ~Ⅳ期与有淋巴结转移患者,差异有统计学意义(均P <0.05)。Ⅰ~Ⅱ期与无淋巴结转移的乳腺癌患者血清CA153水平分别低于Ⅲ~Ⅳ期与有淋巴结转移患者,差异有统计学意义(均P <0.05)。miR-6861-5p联合CA153检测诊断乳腺癌的敏感度、特异度均高于miR-6861-5p与CA153水平单独检测(均P <0.05)。结论血清miR-6861-5p联合CA153检测应用于乳腺癌患者中具有较高的诊断效能,可作为临床上诊断乳腺癌的一种有效手段。
Objective To study the diagnostic value of serum micro RNA-6861-5 p(miR-6861-5 p) combined with carbohydrate antigen 153(CA153) in breast cancer. Methods A total of 83 cases of breast cancer patients admitted to Anshan Tumor Hospital(hereinafter referred to as "our hospital") from April 2015 to April 2018 were selected, which were recorded as the breast cancer group. In addition, 80 patients with benign breast diseases who received diagnosis and treatment in our hospital during the same period were selected as the benign breast disease group, and 80 healthy people who underwent physical examination in our hospital during the same period were selected as the control group.Real-time fluorescence quantitative polymerase chain reaction was used to detect the expression of serum miR-6861-5 p in each group, and the serum CA153 level was detected by chemiluminescence. The levels of serum miR-6861-5 p and CA153 in the three groups were compared, and the relationship between the above two indicators and the clinicopathological characteristics of breast cancer patients was analyzed. The diagnostic efficacy of serum miR-6861-5 p combined with CA153 levels in breast cancer was analyzed using the receiver operating characteristic(ROC) curve. Results The levels of serum miR-6861-5 p and CA153 in the breast cancer group were higher than those in the benign breast disease group and the control group, with statistically significant differences(all P < 0.05). Ⅰ-Ⅱ period with no lymph node metastasis of breast cancer patients serum miR-6861-5 P levels were lower than Ⅲ-Ⅳ period and the patients with lymph node metastasis, the differences were statistically significant(all P < 0.05). Ⅰ-Ⅱ period with no lymph node metastasis of breast cancer patients serum CA153 levels were lower than Ⅲ-Ⅳ period and the patients with lymph node metastasis, the differences were statistically significant(all P < 0.05). The sensitivity and specificity under the curve of miR-6861-5 p combined with CA153 in diagnosis of breast cancer were all higher than those of miR-6861-5 p and CA153 alone(all P < 0.05). Conclusion The application of serum miR-6861-5 p combined with CA153 detection in breast cancer patients has high diagnostic efficacy, and can be used as an effective means for clinical diagnosis of breast cancer.
引文
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