壳寡糖对小鼠急性酒精性肝损伤的保护作用
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摘要
本实验研究了壳寡糖对急性酒精性肝损伤的保护作用。建立小鼠急性肝损伤模型,连续灌胃受试物(壳寡糖)30 d后给予12 mL/kg mb的体积分数50%乙醇溶液后处死,对小鼠进行了肝脏病理组织学检查,并测定了血清中谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartate aminotransferase,AST)活力,总胆固醇(total cholesterol,TC)浓度,以及肝脏系数、肝脏中谷胱甘肽(glutathione,GSH)含量、超氧化物歧化酶(superoxide dismutase,SOD)活力和丙二醛(malondial dehyde,MDA)含量。结果显示,与酒精模型组相比,壳寡糖组小鼠肝组织损伤程度有所减轻,阳性对照组和壳寡糖组血清ALT、AST和TC水平显著降低,肝脏GSH含量和SOD活力均显著增加,而肝脏系数和MDA含量显著降低(P<0.05),表明壳寡糖对小鼠酒精性肝损伤有明显的保护作用。
In this paper,the protective effect of chitooligosaccharides(COS) on acute alcohol-induced liver injury was studied.A mouse model of acute liver injury was established by administering 50% ethanol at a dose of 12 mL/kg m_b to mice after 30 continuous days of oral gavage with COS.The mice were sacrificed for histopathological examination of liver tissue.Besides,the activity of alanine aminotransferase(ALT) and aspartate aminotransferase(AST) and total cholesterol(TC) concentration in serum,liver coefficient,and glutathione(GSH) content,superoxide dismutase(SOD) activity and malondialdehyde(MDA) content in liver were detected.The results showed that the degree of liver injury in the COS group was mitigated compared with the alcohol model group.The positive control and COS groups exhibited a significant reduction in serum ALT,AST and TC levels and a significant increase in hepatic GSH content and SOD activity as well as a significant decrease in liver index and MDA level(P<0.05).Therefore,chitooligosaccharides has a significant protective effect on alcohol-induced liver injury in mice.
In this paper,the protective effect of chitooligosaccharides(COS) on acute alcohol-induced liver injury was studied.A mouse model of acute liver injury was established by administering 50% ethanol at a dose of 12 mL/kg m_b to mice after 30 continuous days of oral gavage with COS.The mice were sacrificed for histopathological examination of liver tissue.Besides,the activity of alanine aminotransferase(ALT) and aspartate aminotransferase(AST) and total cholesterol(TC) concentration in serum,liver coefficient,and glutathione(GSH) content,superoxide dismutase(SOD) activity and malondialdehyde(MDA) content in liver were detected.The results showed that the degree of liver injury in the COS group was mitigated compared with the alcohol model group.The positive control and COS groups exhibited a significant reduction in serum ALT,AST and TC levels and a significant increase in hepatic GSH content and SOD activity as well as a significant decrease in liver index and MDA level(P<0.05).Therefore,chitooligosaccharides has a significant protective effect on alcohol-induced liver injury in mice.
引文
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[19]周丽萍,张悦,王化,等.蓓蕾蓝靛果花色苷对乙醇诱导小鼠氧化损伤的保护作用[J].食品工业科技,2017,38(19):293-297.DOI:10.13386/j.issn1002-0306.2017.19.054.
[20]DING R B,TIAN K,CAO Y W,et al.Protective effect of panax notoginseng saponins on acute ethanol-induced liver injury is associated with ameliorating hepatic lipid accumulation and reducing ethanol-mediated oxidative stress[J].Journal of Agricultural and Food Chemistry,2015,63(9):2413-2422.DOI:10.1021/jf502990n.
[21]田姮.蓝莓解酒护肝作用及其机理的研究[D].沈阳:沈阳农业大学,2017:5-13.
[22]朱良,赵冠欣,沈耀威,等.橄榄总黄酮对小鼠急性酒精性肝损伤的保护作用[J].食品与机械,2010,26(3):91-93.DOI:10.3969/j.issn.1003-5788.2010.03.026.
[23]LUO Z,DONG X,KE Q,et al.Chitooligosaccharides inhibit ethanolinduced oxidative stress via activation of Nrf2 and reduction of MAPKphosphorylation[J].Oncology Reports,2014,32(5):2215-2222.DOI:10.3892/or.2014.3463.
[24]CAO Y W,JIANG Y,ZHANG D Y,et al.The hepatoprotective effect of aqueous extracts of Penthorum chinense Pursh against acute alcohol-induced liver injury is associated with ameliorating hepatic steatosis and reducing oxidative stress[J].Food Function,2015,6(5):1510-1517.DOI:10.1039/c5fo00098j.
[25]WU D,CEDERBAUM A I.Oxidative stress mediated toxicity exerted by ethanol-inducible CYP2E1[J].Toxicology and Applied Pharmacology,2005,207:70-76.DOI:10.1016/j.taap.2005.01.057.
[26]SONG S,LI S L,SU N N,et al.Structural characterization,molecular modification and hepatoprotective effect of melanin from Lachnum YM226 on acute alcohol-induced liver injury in mice[J].Food&Function,2016,7(8):3617-3627.DOI:10.1039/C6FO00333H.
[27]张志群,陈宏辉.核转录因子Nrf2与肝脏疾病的研究进展[J].中国现代医药杂志,2014,16(9):99-102.DOI:10.3969/j.issn.1672-9463.2014.09.045.
[28]王茜.Nrf2/ARE信号通路在纳米二氧化钛诱导HepG2细胞氧化损伤中的作用研究[D].石家庄:河北医科大学,2014:10-43.
[29]ARORA S,KAUR I P,CHOPRA K,et al.Efficiency of double layered microencapsulated probiotic to modulate proinflammatory molecular markers for the management of alcoholic liver disease[J].Mediators Inflamm,2014,2014:715130.DOI:10.1155/2014/715130.
[30]MENDIS E,KIM M M,RAJAPAKSE N,et al.An in vitro cellular analysis of the radical scavenging efficacy of chitooligosaccharides[J].Life Sciences,2007,80(23):2118-2127.DOI:10.1016/j.lfs.2007.03.016.
[31]KONERU M,SAHU B D,GUDEM S,et al.Polydatin alleviates alcohol-induced acute liver injury in mice:relevance of matrix metallo proteinases(MMPs)and hepatican tioxidants[J].Phytomedicine,2017,27:23-32.DOI:10.1016/j.phymed.2017.01.013.
[2]段培鲁.筛选中药提取物及联合葡醛内酯醒酒活性和机理研究[D].合肥:安徽大学,2013:3-8.
[3]刘亮亮,刘万顺,韩宝芹,等.壳寡糖、氨基葡萄糖对酒精性肝损伤小鼠的保护作用[J].中国海洋大学学报(自然科学版),2010,40(7):73-76.DOI:10.3969/j.issn.1672-5174.2010.07.012.
[4]梁慧培,覃小丽,钟金锋.壳寡糖制备和生理活性的研究进展[J].食品工业科技,2016,37(3):384-388.DOI:10.13386/j.issn1002-0306.2016.03.072.
[5]闫海侠,刘佳,孟祥红.壳寡糖对大肠杆菌抑菌作用的研究[J].农产品加工(学刊),2014(7):1-4;7.DOI:10.3969/jissn.1671-9646(X).2014.04.001.
[6]张筠,杜鹏,张亚东.壳寡糖抑菌作用的研究[J].食品工业科技,2009,30(1):88-90.
[7]吴玉潇,徐海涛,高云华,等.壳寡糖的生物活性研究进展[J].明胶科学与技术,2015,35(3):128-132.DOI:10.3969/j.issn.1004-9657.2015.03.004.
[8]胡迎青,陆雪华,汪晓莺,等.壳寡糖降血脂作用及与RAP的表达相关性研究[J].中国临床医学,2007,14(6):749-752.DOI:10.3969/j.issn.1008-6358.2007.06.002.
[9]王斌,苏谦俊,张思聪,等.壳寡糖和壳聚糖对高脂膳食小鼠的降脂功能比较研究[J].天然产物研究与开发,2017,29(6):1025-1029;1047.DOI:10.16333/j.1001-6880.2017.6.021.
[10]LIU B,LIU W S,HAN B Q,et al.Antidiabetic effects of chitooligosaccharides on pancreatic islet cells in streptozotocininduced diabetic rats[J].World Journal of Gastroenterology,2007,13(5):725-731.DOI:10.3748/wjg.v13.i5.725.
[11]王鑫,林强,田平芳,等.低分子量壳寡糖改善II型糖尿病大鼠症状及其作用机制研究[J].食品科学,2007,28(11):529-532.DOI:10.3321/j.issn:1002-6630.2007.11.128.
[12]刘红云,覃彩芹,李顺文,等.壳寡糖对急性酒精性肝损伤保护作用的研究[J].中国现代医学杂志,2010,20(3):350-353.DOI:10.3969/j.issn.1005-8982.2010.03.008.
[13]CHO S Y,YUN J W,PARK P J.Effects of chitooligosaccharide lactate salt on activity of acetaldehyde dehydrogenase[J].Journal of Medicinal Food,2010,13(5):1061-1068.DOI:10.1089/jmf.2009.1323.
[14]何慧,石燕玲,徐淑芬,等.灵芝肽对乙醇诱导肝损伤小鼠的保护作用[J].食品科学,2010,31(3):213-216.
[15]WANG Z,SU B,FAN S,et al.Protective effect of oligomeric proanthocyanidins against alcohol-induced liver steatosis and injury in mice[J].Biochemical and Biophysical Research Communications,2015,458(4):757-762.DOI:10.1016/j.bbrc.2015.01.153.
[16]ZHANG N,HU Y,DING C,et al.Salvianolic acid B protects against chronic alcoholic liver injury via SIRT1-mediated inhibition of CRP and ChREBP in rats[J].Toxicology Letters,2017,267:1-10.DOI:10.1016/j.toxlet.2016.12.010.
[17]LI C,LI L,YANG C F,et al.Hepatoprotective effects of methyl ferulic acid on alcohol-induced liver oxidative injury in mice by inhibiting the NOX4/ROS-MAPK pathway[J].Biochemical and Biophysical Research Communications,2017,493(1):277-285.DOI:10.1016/j.bbrc.2017.09.030.
[18]WANG M,ZHU P,JIANG C,et al.Preliminary characterization,antioxidant activity in vitro and hepatoprotective effect on acute alcohol-induced liver injury in mice of polysaccharides from the peduncles of Hovenia dulcis[J].Food and Chemical Toxicology,2012,50(9):2964-2970.DOI:10.1016/j.fct.2012.06.034.
[19]周丽萍,张悦,王化,等.蓓蕾蓝靛果花色苷对乙醇诱导小鼠氧化损伤的保护作用[J].食品工业科技,2017,38(19):293-297.DOI:10.13386/j.issn1002-0306.2017.19.054.
[20]DING R B,TIAN K,CAO Y W,et al.Protective effect of panax notoginseng saponins on acute ethanol-induced liver injury is associated with ameliorating hepatic lipid accumulation and reducing ethanol-mediated oxidative stress[J].Journal of Agricultural and Food Chemistry,2015,63(9):2413-2422.DOI:10.1021/jf502990n.
[21]田姮.蓝莓解酒护肝作用及其机理的研究[D].沈阳:沈阳农业大学,2017:5-13.
[22]朱良,赵冠欣,沈耀威,等.橄榄总黄酮对小鼠急性酒精性肝损伤的保护作用[J].食品与机械,2010,26(3):91-93.DOI:10.3969/j.issn.1003-5788.2010.03.026.
[23]LUO Z,DONG X,KE Q,et al.Chitooligosaccharides inhibit ethanolinduced oxidative stress via activation of Nrf2 and reduction of MAPKphosphorylation[J].Oncology Reports,2014,32(5):2215-2222.DOI:10.3892/or.2014.3463.
[24]CAO Y W,JIANG Y,ZHANG D Y,et al.The hepatoprotective effect of aqueous extracts of Penthorum chinense Pursh against acute alcohol-induced liver injury is associated with ameliorating hepatic steatosis and reducing oxidative stress[J].Food Function,2015,6(5):1510-1517.DOI:10.1039/c5fo00098j.
[25]WU D,CEDERBAUM A I.Oxidative stress mediated toxicity exerted by ethanol-inducible CYP2E1[J].Toxicology and Applied Pharmacology,2005,207:70-76.DOI:10.1016/j.taap.2005.01.057.
[26]SONG S,LI S L,SU N N,et al.Structural characterization,molecular modification and hepatoprotective effect of melanin from Lachnum YM226 on acute alcohol-induced liver injury in mice[J].Food&Function,2016,7(8):3617-3627.DOI:10.1039/C6FO00333H.
[27]张志群,陈宏辉.核转录因子Nrf2与肝脏疾病的研究进展[J].中国现代医药杂志,2014,16(9):99-102.DOI:10.3969/j.issn.1672-9463.2014.09.045.
[28]王茜.Nrf2/ARE信号通路在纳米二氧化钛诱导HepG2细胞氧化损伤中的作用研究[D].石家庄:河北医科大学,2014:10-43.
[29]ARORA S,KAUR I P,CHOPRA K,et al.Efficiency of double layered microencapsulated probiotic to modulate proinflammatory molecular markers for the management of alcoholic liver disease[J].Mediators Inflamm,2014,2014:715130.DOI:10.1155/2014/715130.
[30]MENDIS E,KIM M M,RAJAPAKSE N,et al.An in vitro cellular analysis of the radical scavenging efficacy of chitooligosaccharides[J].Life Sciences,2007,80(23):2118-2127.DOI:10.1016/j.lfs.2007.03.016.
[31]KONERU M,SAHU B D,GUDEM S,et al.Polydatin alleviates alcohol-induced acute liver injury in mice:relevance of matrix metallo proteinases(MMPs)and hepatican tioxidants[J].Phytomedicine,2017,27:23-32.DOI:10.1016/j.phymed.2017.01.013.