生物素衍生物自组装膜制备及其覆盖度的电化学测定
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摘要
利用循环伏安法和电化学交流阻抗谱研究了MBDA/Au,MBTA/Au,TBDA/Au,TBTA/Au修饰电极以及MBDA与DT混合自组装/Au、TBTA与DT混合自组装/Au修饰电极的覆盖度。结果表明这些自组装膜在金电极表面的覆盖度均比较高,达到99%以上;对同一类修饰电极,混合自组装膜的覆盖度高于单组分自组装膜的覆盖度,混合自组装体系的覆盖度随着DT比例的增加而增加;同类物质,长链化合物在金表面的覆盖度大于短链化合物,含二硫键的化合物与金电极的键合能力比含一个巯基的化合物键合能力强。
The electrochemical cyclic voltammetry and electrochemical impendence spectra were employed to characterize the coverage of the pure SAMs of MBDA/Au,MBTA/Au,TBDA/Au and TBTA/Au.Meanwhile,using 11.11′-diundecanolthiol(DT)as the medium molecule,the mixed SAMs of MBDA/DT and TBTA/DT on gold were also fabricated.The results showed that closely packed and well ordered SAMs had been fabricated and their surface coverages were over 99%.Mixed SAMs had higher coverages than the corresponding pure SAMs,and the coverage increases with the increase of the molar ratio of the medium molecule DT.At the same time,with the lengthening of the ether linkage between thioctic acid or 11-mercaptoundecanoic acid and biotin,the SAMs would have a greater surface coverage.
The electrochemical cyclic voltammetry and electrochemical impendence spectra were employed to characterize the coverage of the pure SAMs of MBDA/Au,MBTA/Au,TBDA/Au and TBTA/Au.Meanwhile,using 11.11′-diundecanolthiol(DT)as the medium molecule,the mixed SAMs of MBDA/DT and TBTA/DT on gold were also fabricated.The results showed that closely packed and well ordered SAMs had been fabricated and their surface coverages were over 99%.Mixed SAMs had higher coverages than the corresponding pure SAMs,and the coverage increases with the increase of the molar ratio of the medium molecule DT.At the same time,with the lengthening of the ether linkage between thioctic acid or 11-mercaptoundecanoic acid and biotin,the SAMs would have a greater surface coverage.
引文
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[3]Valber A Pedrosa,Thiago R L C Paixlio,Renato S Freire,et a1.Studies on the electrochemical behavior of a cystineself-assembled monolayer modified electrode using ferro-cyanide as a probe[J].J Electroanal Chem,2007,602(2):149-155.
[4]左国防,袁妮,潘素娟.基于纳米金自组装膜固定辣根过氧化物酶的生物传感研究[J].化学研究与应用,2012,24(1):84-89.
[5]杨明蓉,唐卓,陈应春.生物素标记试剂的合成方法改进及其在DNA合成中的应用[J].合成化学2012,20(3):331-333.
[6]赵俊芳,王学谦,李桂珍.生物素亲和素系统在ECP的实验效能[J].天津医药,2012,40(3):221-223.
[7]张俊,苓杨芳,郑文杰,等.自组装单分子膜及其表征方法[J].化学进展,2005,17(2):203-208.
[8]郭敏,刁鹏,童汝亭.金/巯基环肽单层膜的循环伏安和交流阻抗初步研究[J].化学研究与应用2001,13(6):641-644.
[9]Diao P,Jiang D L,Cui X L,et al.Study of structural disor-der of self-assembled thiol monolayers on gold by cyclicvoltammetry and ac impedance[J].J Electroanal Chem,1999,464:61-67.
[10]Fedurco,M.Redox reactions of heme-containing metallo-proteins:dynamic effects of self-assembled monolayers onthermodynamics and kinetics of cytochrome c electron-transfer reactions[J].Coord Chem Rev,2000,209(1):263-269.
[11]Sabatani,E,Rubinstain,I,Maoz,R.etOrganized self-as-sembling monolayerson electrodes part I Octadecyl deriv-atives on gold.[J].J Electroanal Chem,1987,219(1-2):365-371.
[12]Eyal S,Joseph C,Merlin B,et al,Thioaromatic monolay-ers on gold:a new family of self-assembling monolayers[J].Langmuir,1993,9:2 974-2 981.
[13]Campbell A A,Fryxell G E,Graff G L,et al.The nuclea-tion and growth of calcium oxalate monohydrate on self-assembled monolayers[J].Scanning Microsc,1993,7(1):423-429.