干细胞技术对肝细胞癌的影响
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摘要
干细胞技术可分为正常干细胞技术和癌干细胞技术,骨髓间充质干细胞(BMSC)移植可促进肝组织再生,诱导肝癌细胞凋亡,但BMSC具有潜在的致瘤性。肝癌组织对BMSC具有趋化性,可定向分化肝细胞或肝癌细胞。肝癌细胞可来源于肝癌干细胞,肝前体细胞可分化为肝癌干细胞。肝癌细胞与干细胞相互影响、相互作用中发生的变化是肿瘤或干细胞演变的重要事件,对其深入研究将对基于干细胞技术的肿瘤生物治疗起到积极作用。
Stem cell technology can be divided into the normal stem cells and cancer stem cells technology. Bone marrow mesenchymal stem cells( BMSC) transplantation can promote liver tissue regeneration and induce tumor cells apoptosis. But BMSC has potential tumorigenicity. BMSC has a tendency of chemotaxis in HCC tissues and can be directedly differentiated into liver cells or liver cancer cells. Liver cancer cells can be derived from liver cancer stem cells. Hepatic progenitor cells can be differentiated into liver cancer stem cells. Changes are the important events in the evolution of the tumor or stem cells in the interaction between liver cancer cells and stem cells,the in-depth study on which will play a positive role in biological therapy based on stem cell technology.
Stem cell technology can be divided into the normal stem cells and cancer stem cells technology. Bone marrow mesenchymal stem cells( BMSC) transplantation can promote liver tissue regeneration and induce tumor cells apoptosis. But BMSC has potential tumorigenicity. BMSC has a tendency of chemotaxis in HCC tissues and can be directedly differentiated into liver cells or liver cancer cells. Liver cancer cells can be derived from liver cancer stem cells. Hepatic progenitor cells can be differentiated into liver cancer stem cells. Changes are the important events in the evolution of the tumor or stem cells in the interaction between liver cancer cells and stem cells,the in-depth study on which will play a positive role in biological therapy based on stem cell technology.
引文
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[15]Zhou YF,Bosch-Marce M,Okuyama H,et al.Spontaneous transformation of cultured mouse bone marrow-derived stromalcells[J].Cancer Research,2006,66(22):10849-10854.
[16]Tasso R,Augello A,Carida M,et al.Development of sarcomas in mice implanted with mesenchymal stem cells seeded onto bioscaffolds[J].Carcinogenesis,2009,30(1):150-157.
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[28]Holczbauer A,Factor VM,Andersen JB,et al.Modeling pathogenesis of primary liver cancer in lineage-specific mouse cell types[J].Gastroenterology,2013,145(1):221-231.
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[31]Duncan AW,Dorrell C,Grompe M.Stem cells and liver regeneration[J].Gastroenterology,2009,137(2):466-481.
[32]Chiba T,Kita K,Zheng YW,et al.Side population purified from hepatocellular ca rcinoma cells harbors cancer stem cell-like properties[J].Hepatology,2006,44(1):240-251.
[33]Lowes KN,Brennan BA,Yeoh GC,et al.Oval cell numbers in human chronic liver diseases are directly related to disease severity[J].Am J Pathol,1999,154(2):537-541.
[34]Williams JM,Oh SH,Jorgensen M,et al.The role of the Wnt family of secreted proteins in rat oval"stem"cellbased liver regeneration:Wnt1 drives differentiation[J].Am J Pathol,2010,176(6):2732-2742.
[35]Xu S,Tong M,Huang J,et al.TRIB2 inhibits Wnt/β-Catenin/TCF4 signaling through its associated ubiquitin E3 ligases,β-Tr CP,COP1 and Smurf1,in liver cancer cells[J].FEBS Lett,2014,588(23):4334-4341.
[36]Song L,Li ZY,Liu WP,et al.Crosstalk between Wnt/β-catenin and Hedgehog/Gli signaling pathways in colon cancer and implications for therapy[J].Cancer Biol Ther,2015;16(1):1-7.
[37]Geissler K,Zach O.Pathways involved in Drosophila and human cancer development:the Notch,Hedgehog,Wingless,Runt,and Trithorax pathway[J].Ann Hematol,2012,91(5):645-669.
[38]Li Y,Jiang F,Chen L,et al.Blockage of TGFβ-SMAD2 by demethylation-activated miR-148a is involved in caffeic acid-induced inhibition of cancer stem cell-like properties in vitro and in vivo[J].FEBS Open Bio,2015(5):466-475.
[39]Sekiya S,Suzuki A.Intrahepatic cholangiocarcinoma can arise from Notch-mediated conversion of hepatocytes[J].J Clin Invest,2012,122(11):3914-3918.
[2]Vainshtein JM,Kabarriti R,Mehta KJ,et al.Bone marrow-derived stromal cell therapy in cirrhosis:clinical evidence,cellular mechanisms,and implications for the treatment of hepatocellular carcinoma[J].Int J Radiat Oncol Biol Phys,2014,89(4):786-803.
[3]Petersen BE,Bowen WC,Patrene KD,et al.Bone marrow as a potential source of hepatic oval cells[J].Science,1999,284(5417):1168-1170.
[4]Al Ahmari LS,Al Shenaifi JY,Al Anazi RA,et al.Autologous bone marrow-derived cells in the treatment of liver disease patients[J].Saudi J Gastroenterol,2015,21(1):5-10.
[5]Liu WH,Song FQ,Ren LN,et al.The multiple functional roles of mesenchymal stem cells in participating in treating liver diseases[J].J Cell Mol Med,2015,19(3):511-520.
[6]Terai S,Sakaida I,Nishina H,et al.Lesson from the GFP/CCl4model--translational research project:the development of cell therapy using autologous bone marrow cells in patients with liver cirrhosis[J].J Hepatobiliary Pancreat Surg,2005,12(3):203-207.
[7]Aquino JB,Bolontrade MF,Garcia MG,et al.Mesenchymal stem cells as therapeutic tools and gene carriers in liver fibrosis and hepatocellular carcinoma[J].Gene Ther,2010,17(6):692-708.
[8]Dwyer RM,Kerin MJ.Mesenchymal stem cells and cancer:tumorspecific delivery vehicles or therapeutic targets?[J].Hum Gene Ther,2010,21(11):1506-1512.
[9]周佳美.大鼠骨髓间充质干细胞与CBRH--7919肝癌细胞相互作用的研究[D].天津:天津医科大学,2012.
[10]Li GC,Ye QH,Xue YH,et al.Human mesenchymal stem cells inhibit metastasis of a hepatocellular carcinoma model using the MHCC97-H cell line[J].Cancer Sci,2010,101(12):2546-2553.
[11]Gong P,Wang Y,Wang Y,et al.Effect of bone marrow mesenchymal stem cells on hepatocellular carcinoma in microcirculation[J].Tumour Biol,2013,34(4):2161-2168.
[12]Li T,Song B,Du X,et al.Effect of bone-marrow-derived mesenchymal stem cells on high-potential hepatocellular carcinoma in mouse models:an intervention study[J].Eur J Med Res,2013,18(1):34.
[13]周彬,陈志浩,宫绪萌,等.骨髓干细胞移植治疗肝细胞癌的研究进展[J].中国医药导报,2014,11(22):151-153.
[14]Wang Y,Huso DL,Harrington J,et al.Outgrowth of a transformed cell population derived from normal human BM mesenchymal stem cell culture[J].Cytotherapy,2005,7(6):509-519.
[15]Zhou YF,Bosch-Marce M,Okuyama H,et al.Spontaneous transformation of cultured mouse bone marrow-derived stromalcells[J].Cancer Research,2006,66(22):10849-10854.
[16]Tasso R,Augello A,Carida M,et al.Development of sarcomas in mice implanted with mesenchymal stem cells seeded onto bioscaffolds[J].Carcinogenesis,2009,30(1):150-157.
[17]胡宜,程鹏,刘云会.骨髓间充质干细胞应用的风险:促瘤及成瘤性[J].解剖科学进展,2011,17(5):492-495,498.
[18]Aguilar S,Nye E,Chan J,et al.Murine but not human mesenchymal stem cells generate osteosarcoma-like lesions in the lung[J].Stem Cells,2007,25(6):1586-1594.
[19]Bruno S,Collino F,Deregibus MC,et al.Microvesicles derived from human bone marrow mesenchymal stem cells inhibit tumor growth[J].Stem Cells Dev,2013,22(5):758-771.
[20]Jin H,Aiyer A,Su J,et al.A homing mechanism for bone marrowderived progenitor cell recruitment to theneovasculature[J].J Clin Inv,2006,116(3):652-662.
[21]Tajima F,Tsuchiya H,Nishikawa K,et al.Hepatocyte growth factor mobilizes and recruits hematopoietic progenitor cells into liver through a stem cell factor-mediated mechanism[J].Hepatol Res,2010,40(7):711-719.
[22]李涛,陈娟,李学华,等.应用SRY原位杂交技术检测肝脏干细胞移植肝内种植情况[J].中国组织工程研究,2012,16(53):9881-9886.
[23]宋浩,纪卫政,邰沁文,等.骨髓间充质干细胞对肝癌模型大鼠血管形成的影响[J].中国组织工程研究,2012,16(14):2481-2486.
[24]王阁,索金友,邓婧,等.原发性肝癌不同病理组织类型中肝干细胞的起源分析[J].第三军医大学学报,2006,28(2):114-116.
[25]Bruux J,Llovet JM.Major achievements in hepatocellular carcinoma[J].The Lancet,2009,373(9664):614.
[26]庞业滨,黎乐群,彭宁福,等.肝癌细胞起源的研究现状[J].医学综述,2014,20(12):2146-2148.
[27]Ajani JA,Song S,Hochster HS,et al.Cancer Stem Cells:The Promise and the Potential[J].Semin Oncol,2015,42 Suppl 1:S3-17.
[28]Holczbauer A,Factor VM,Andersen JB,et al.Modeling pathogenesis of primary liver cancer in lineage-specific mouse cell types[J].Gastroenterology,2013,145(1):221-231.
[29]Farber E.Carcinoma of the liver in rats fed ethionine[J].AMA Arch Pathol,1956,62(6):445-453.
[30]Oertel M,Shafritz DA.Stem cells,cell transplantation and liver repopulation[J].Biochim Biophys Acta,2008,1782(2):61-74.
[31]Duncan AW,Dorrell C,Grompe M.Stem cells and liver regeneration[J].Gastroenterology,2009,137(2):466-481.
[32]Chiba T,Kita K,Zheng YW,et al.Side population purified from hepatocellular ca rcinoma cells harbors cancer stem cell-like properties[J].Hepatology,2006,44(1):240-251.
[33]Lowes KN,Brennan BA,Yeoh GC,et al.Oval cell numbers in human chronic liver diseases are directly related to disease severity[J].Am J Pathol,1999,154(2):537-541.
[34]Williams JM,Oh SH,Jorgensen M,et al.The role of the Wnt family of secreted proteins in rat oval"stem"cellbased liver regeneration:Wnt1 drives differentiation[J].Am J Pathol,2010,176(6):2732-2742.
[35]Xu S,Tong M,Huang J,et al.TRIB2 inhibits Wnt/β-Catenin/TCF4 signaling through its associated ubiquitin E3 ligases,β-Tr CP,COP1 and Smurf1,in liver cancer cells[J].FEBS Lett,2014,588(23):4334-4341.
[36]Song L,Li ZY,Liu WP,et al.Crosstalk between Wnt/β-catenin and Hedgehog/Gli signaling pathways in colon cancer and implications for therapy[J].Cancer Biol Ther,2015;16(1):1-7.
[37]Geissler K,Zach O.Pathways involved in Drosophila and human cancer development:the Notch,Hedgehog,Wingless,Runt,and Trithorax pathway[J].Ann Hematol,2012,91(5):645-669.
[38]Li Y,Jiang F,Chen L,et al.Blockage of TGFβ-SMAD2 by demethylation-activated miR-148a is involved in caffeic acid-induced inhibition of cancer stem cell-like properties in vitro and in vivo[J].FEBS Open Bio,2015(5):466-475.
[39]Sekiya S,Suzuki A.Intrahepatic cholangiocarcinoma can arise from Notch-mediated conversion of hepatocytes[J].J Clin Invest,2012,122(11):3914-3918.