妊高症患者胎盘MiR-26b和Nkx2-5的表达水平及临床意义
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摘要
目的探究妊娠高血压疾病患者胎盘micro RNA-26b(MiR-26b)和Nkx2-5的表达水平及临床意义。方法选择2016年3月～2017年10月于笔者医院接受治疗的60例妊高症患者(包括20例妊娠期高血压、20例轻度子痫前期和20例重度子痫前期)为实验组,另选择同期住院的20例健康孕妇作为对照组。实时定量PCR(q RT-PCR)技术检测胎盘组织MiR-26b水平;蛋白质印迹(Western blot)技术检测胎盘组织Nkx2-5水平。结果轻度子痫前期组和重度子痫前期组的新生儿体重及胎盘重量较健康对照组均显著降低(P<0.05)。q RT-PCR结果显示,妊娠期高血压组、轻度子痫前期组和重度子痫前期组胎盘组织MiR-26b水平较健康对照组均显著升高(P<0.05),且随着病情的加重,MiR-26b水平逐渐上升(P<0.05)。Western blot法检测结果显示,妊娠期高血压组、轻度子痫前期组和重度子痫前期组胎盘组织Nkx2-5水平较健康对照组均显著降低(P<0.05),且随着病情的加重,MiR-26b水平逐渐降低(P<0.05)。妊高症患者胎盘组织MiR-26b水平与Nkx2-5水平显著呈负相关(P<0.05)。妊高症患者胎盘组织MiR-26b水平与新生儿体重、胎盘重量呈负相关(P<0.05),Nkx2-5水平与新生儿体重、胎盘重量显著呈正相关(P<0.05)。结论妊高症患者胎盘组织中MiR-26b表达上调、Nkx2-5表达下调,且与病情严重程度相关,暗示MiR-26b、Nkx2-5可作为妊高症的独立诊断因子。
Objective To explore the expression level and clinical significance of placenta micro RNA-26 b(MiR-26 b) and Nkx2-5 in patients with pregnancy-induced hypertension.Methods Sixty cases of pregnancy-induced hypertension patients from March2016 to October 2017 treated in author's hospital(including twenty cases of gestational hypertension,twenty cases of mild preeclampsia and twenty cases of severe preeclampsia) were selected as the experimental group,and twenty healthy pregnant women were selected as control group at the same time.Real time quantitative PCR(q RT-PCR) technique was used to detect the placental tissue MiR-26 b level;Western blot technique was used to detect the placental tissue Nkx2-5 level.Results The neonatal weight and placental weigh in mild preeclampsia group and severe preeclampsia group were significantly lower than those in healthy control group(P<0.05).The q RT-PCR results showed that placental tissue MiR-26 b levels in gestational hypertension group,mild preeclampsia group and severe preeclampsia group were significantly higher than the healthy control group(P<0.05),and with the aggravation of the disease,the level gradually increased(P<0.05).The Western blot results showed that gestational hypertension group,mild preeclampsia and severe preeclampsia group placental tissue Nkx2-5 levels were significantly lower than the healthy control group(P<0.05),and with the aggravation of the disease,the level of MiR-26 b decreased gradually(P<0.05).There was a negative correlation between the MiR-26 b level and the Nkx2-5 level in placental tissue of PIH(P<0.05).The placenta tissue MiR-26 b level was negatively correlated with neonatal weight and placental weight(P<0.05),and the Nkx2-5 level was positively correlated with neonatal weight and placental weight(P<0.05).Conclusion The expression of MiR-26 b is up-regulated and the expression of Nkx2-5 is down-regulated in placenta of PIH patients,which are related to the severity of the disease.It suggests that MiR-26 b and Nkx2-5 can be used as independent diagnostic factors for pregnancy-induced hypertension.
Objective To explore the expression level and clinical significance of placenta micro RNA-26 b(MiR-26 b) and Nkx2-5 in patients with pregnancy-induced hypertension.Methods Sixty cases of pregnancy-induced hypertension patients from March2016 to October 2017 treated in author's hospital(including twenty cases of gestational hypertension,twenty cases of mild preeclampsia and twenty cases of severe preeclampsia) were selected as the experimental group,and twenty healthy pregnant women were selected as control group at the same time.Real time quantitative PCR(q RT-PCR) technique was used to detect the placental tissue MiR-26 b level;Western blot technique was used to detect the placental tissue Nkx2-5 level.Results The neonatal weight and placental weigh in mild preeclampsia group and severe preeclampsia group were significantly lower than those in healthy control group(P<0.05).The q RT-PCR results showed that placental tissue MiR-26 b levels in gestational hypertension group,mild preeclampsia group and severe preeclampsia group were significantly higher than the healthy control group(P<0.05),and with the aggravation of the disease,the level gradually increased(P<0.05).The Western blot results showed that gestational hypertension group,mild preeclampsia and severe preeclampsia group placental tissue Nkx2-5 levels were significantly lower than the healthy control group(P<0.05),and with the aggravation of the disease,the level of MiR-26 b decreased gradually(P<0.05).There was a negative correlation between the MiR-26 b level and the Nkx2-5 level in placental tissue of PIH(P<0.05).The placenta tissue MiR-26 b level was negatively correlated with neonatal weight and placental weight(P<0.05),and the Nkx2-5 level was positively correlated with neonatal weight and placental weight(P<0.05).Conclusion The expression of MiR-26 b is up-regulated and the expression of Nkx2-5 is down-regulated in placenta of PIH patients,which are related to the severity of the disease.It suggests that MiR-26 b and Nkx2-5 can be used as independent diagnostic factors for pregnancy-induced hypertension.
引文
1林开颜,林浩铨,蔡琍璇,等.新生儿出生结局与孕母妊娠高血压疾病的关系[J].广东医学,2015,36(5):761-762
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5 Mei Z,Huang B,Mo Y,et al.An exploratory study into the role of mi R-204-5p in pregnancy-induced hypertension[J].Exp Therapeut Med,2017,13(5):1711-1718
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9 World Health Organisation.WHO recommendations for prevention and treatment of pre-eclampsia and eclampsia[J].Clin Endocrinol,2011,53(2):260-261
10陈俊宏,姚勤,李光群.重度妊娠高血压疾病终止妊娠时机及方式对母婴预后及并发症的影响[J].实用临床医药杂志,2015,19(9):170-172
11王克涛.硫酸镁联合硝苯地平治疗妊娠高血压综合征的临床效果[J].中国医药导报,2016,13(33):95-98
12刘晓艳.妊娠高血压患者应用综合护理干预进行产后护理的效果评价[J].临床医学研究与实践,2017,2(4):174-175
13 Wang L,Song G,Liu M,et al.Micro RNA-375 overexpression influences P19 cell proliferation,apoptosis and differentiation through the Notch signaling pathway[J].Int J Mol Med,2016,37(1):47-55
14 Jinhyuk B,Hoe-Su J,Soo KJ,et al.PGC-Enriched mi RNAs control germ cell development[J].Mol Cells,2015,38(10):895-903
15 Morgado AL,Xavier JM,Dionísio PA,et al.Micro RNA-34a modulates neural stem cell differentiation by regulating expression of synaptic and autophagic proteins[J].Mol Neurobiol,2015,51(3):1168-1183
16解修峰,史志周.肿瘤及其微环境源性外泌体mi RNAs在致癌机制、肿瘤诊断和治疗中的研究进展[J].基础医学与临床,2017,37(9):1326-1330
17 Muralimanoharan S,Guo C,Myatt L,et al.Sexual dimorphism in mi R-210 expression and mitochondrial dysfunction in the placenta with maternal obesity[J].Int J Obes,2015,39(8):1274-1281
18 Wang Y,Zhang Y,Wang H,et al.Aberrantly up-regulated mi R-20a in pre-eclampsic placenta compromised the proliferative and invasive behaviors of trophoblast cells by targeting forkhead box protein A1.[J].Int J Biol Sci,2014,10(9):973-982
19郝葡萄,宋瑜,王晓娟.Mi R-26b在子痫前期胎盘中差异表达的临床意义[J].中国妇产科临床杂志,2017,18(3):218-220
20李静,荆春丽,陈春梅.产前超声检测胎儿静脉导管与肺静脉血流频谱在评价高危胎儿心功能及不良预后中的应用[J].临床心血管病杂志,2016,32(9):961-964
21余献增,袁华.超声心动图监测孕中晚期胎儿心室Tei指数变化及其与子痫前期的关系[J].中华全科医学,2017,15(1):128-131
22许耘红,何穗镕,李丽青.心脏发育相关基因NKX2.5和GATA4突变与先天性心脏病的相关性[J].中国临床药理学杂志,2016,32(22):2111-2113
2翁海霞.妊娠高血压疾病的相关因素临床分析[J].中国医药导报,2016,13(30):107-110
3徐霞,吴炜,李静,等.循环微小RNA与妊娠的研究进展[J].中国优生与遗传杂志,2014,22(8):9-11
4杨龙江,杜丹,傅强,等.子痫前期相关循环micro RNAs的研究进展[J].分子影像学杂志,2016,39(1):47-50
5 Mei Z,Huang B,Mo Y,et al.An exploratory study into the role of mi R-204-5p in pregnancy-induced hypertension[J].Exp Therapeut Med,2017,13(5):1711-1718
6尹成果,刘学刚.NKX2.5基因在先天性心脏病中的研究进展[J].蚌埠医学院学报,2015,40(11):1608-1611
7谢幸,苟文丽.妇产科学[M].8版.北京:人民卫生出版社,2013:64-70
8任小平,翟艳,何宁.妊娠高血压疾病降压药物应用现状分析[J].海峡药学,2015,27(10):98-100
9 World Health Organisation.WHO recommendations for prevention and treatment of pre-eclampsia and eclampsia[J].Clin Endocrinol,2011,53(2):260-261
10陈俊宏,姚勤,李光群.重度妊娠高血压疾病终止妊娠时机及方式对母婴预后及并发症的影响[J].实用临床医药杂志,2015,19(9):170-172
11王克涛.硫酸镁联合硝苯地平治疗妊娠高血压综合征的临床效果[J].中国医药导报,2016,13(33):95-98
12刘晓艳.妊娠高血压患者应用综合护理干预进行产后护理的效果评价[J].临床医学研究与实践,2017,2(4):174-175
13 Wang L,Song G,Liu M,et al.Micro RNA-375 overexpression influences P19 cell proliferation,apoptosis and differentiation through the Notch signaling pathway[J].Int J Mol Med,2016,37(1):47-55
14 Jinhyuk B,Hoe-Su J,Soo KJ,et al.PGC-Enriched mi RNAs control germ cell development[J].Mol Cells,2015,38(10):895-903
15 Morgado AL,Xavier JM,Dionísio PA,et al.Micro RNA-34a modulates neural stem cell differentiation by regulating expression of synaptic and autophagic proteins[J].Mol Neurobiol,2015,51(3):1168-1183
16解修峰,史志周.肿瘤及其微环境源性外泌体mi RNAs在致癌机制、肿瘤诊断和治疗中的研究进展[J].基础医学与临床,2017,37(9):1326-1330
17 Muralimanoharan S,Guo C,Myatt L,et al.Sexual dimorphism in mi R-210 expression and mitochondrial dysfunction in the placenta with maternal obesity[J].Int J Obes,2015,39(8):1274-1281
18 Wang Y,Zhang Y,Wang H,et al.Aberrantly up-regulated mi R-20a in pre-eclampsic placenta compromised the proliferative and invasive behaviors of trophoblast cells by targeting forkhead box protein A1.[J].Int J Biol Sci,2014,10(9):973-982
19郝葡萄,宋瑜,王晓娟.Mi R-26b在子痫前期胎盘中差异表达的临床意义[J].中国妇产科临床杂志,2017,18(3):218-220
20李静,荆春丽,陈春梅.产前超声检测胎儿静脉导管与肺静脉血流频谱在评价高危胎儿心功能及不良预后中的应用[J].临床心血管病杂志,2016,32(9):961-964
21余献增,袁华.超声心动图监测孕中晚期胎儿心室Tei指数变化及其与子痫前期的关系[J].中华全科医学,2017,15(1):128-131
22许耘红,何穗镕,李丽青.心脏发育相关基因NKX2.5和GATA4突变与先天性心脏病的相关性[J].中国临床药理学杂志,2016,32(22):2111-2113