3种辛味中药挥发油对苦参生物碱类成分透皮吸收的影响
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摘要
研究薄荷、苍术、蛇床子3种辛味药材挥发油对苦参喹诺里西啶类生物碱成分体外透皮吸收的影响。采用立式改良型Franz扩散池,以离体SD大鼠腹部皮肤为透皮吸收屏障进行体外透皮实验,采用UPLC-TQ-MS测定透皮接收液中氧化苦参碱、氧化槐果碱、N-甲基金雀花碱、槐定碱、苦参碱、槐果碱6种喹诺里西啶类生物碱的含量,考察3种挥发油对苦参生物碱类成分透皮吸收的影响。不同质量分数(1%,3%,5%)的3种挥发油对苦参喹诺里西啶类生物碱成分均有透皮促渗作用,促透效果(以ER为指标):1%苍术油>1%蛇床子油>3%氮酮≈3%苍术油>5%苍术油>3%蛇床子油≈5%蛇床子油>3%薄荷油>5%薄荷油>1%薄荷油>空白。结果表明3种挥发油均可作为苦参喹诺里西啶类生物碱成分的促渗剂,为苦参生物碱类成分透皮吸收制剂的研制提供参考。
To investigate the effects of essential oil from three kinds of pungent herbs,namely Menthae Haplocalycis Herba,Atractylodis Rhizoma and Cnidii Fructus,on the transdermal absorption in vitro of alkaloids from Sophorae Flavescentis Radix. The modified vertical Franz diffusion cell was used to conduct a transdermal experiment in vitro with the isolated abdominal skin of the SD rats as the transdermal absorption barrier. The effects of such three kinds of pungent essential oil on percutaneous absorption of alkaloids from Sophorae Flavescentis Radix were investigated by determining the content of 6 alkaloids( oxymatrine,oxysophocarpine,N-methylcytisine,sophoridine,matrine,and sophocarpidine) in the transdermal acceptor with ultra-performance liquid chromatography-triple quadruple mass spectrometry( UPLC-TQ-MS) technique simultaneously. With enhancement ratio( ER) as the index,their effects on promoting penetration was as follows: 1% Atractylodis Rhizoma oil > 1% Cnidii Fructus oil > 3% Azone ≈ 3% Atractylodis Rhizoma oil > 5%Atractylodis Rhizoma oil > 3% Cnidii Fructus oil ≈ 5% Cnidii Fructus oil > 3% Menthae Haplocalycis Herba oil > 5% Menthae Haplocalycis Herba oil > 1% Menthae Haplocalycis Herba oil > Blank. The results showed that these three kinds of pungent essential oil could be used as enhancers for alkaloids of Sophorae Flavescentis Radix,providing scientific guidance for improving percutaneous absorption of alkaloids from Sophorae Flavescentis Radix.
To investigate the effects of essential oil from three kinds of pungent herbs,namely Menthae Haplocalycis Herba,Atractylodis Rhizoma and Cnidii Fructus,on the transdermal absorption in vitro of alkaloids from Sophorae Flavescentis Radix. The modified vertical Franz diffusion cell was used to conduct a transdermal experiment in vitro with the isolated abdominal skin of the SD rats as the transdermal absorption barrier. The effects of such three kinds of pungent essential oil on percutaneous absorption of alkaloids from Sophorae Flavescentis Radix were investigated by determining the content of 6 alkaloids( oxymatrine,oxysophocarpine,N-methylcytisine,sophoridine,matrine,and sophocarpidine) in the transdermal acceptor with ultra-performance liquid chromatography-triple quadruple mass spectrometry( UPLC-TQ-MS) technique simultaneously. With enhancement ratio( ER) as the index,their effects on promoting penetration was as follows: 1% Atractylodis Rhizoma oil > 1% Cnidii Fructus oil > 3% Azone ≈ 3% Atractylodis Rhizoma oil > 5%Atractylodis Rhizoma oil > 3% Cnidii Fructus oil ≈ 5% Cnidii Fructus oil > 3% Menthae Haplocalycis Herba oil > 5% Menthae Haplocalycis Herba oil > 1% Menthae Haplocalycis Herba oil > Blank. The results showed that these three kinds of pungent essential oil could be used as enhancers for alkaloids of Sophorae Flavescentis Radix,providing scientific guidance for improving percutaneous absorption of alkaloids from Sophorae Flavescentis Radix.
引文
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[2]张钟媛.苦参的化学成分和药理作用研究进展[J].云南中医中药杂志,2015,36(6):104.
[3]赵凤春.苦参不同部位化学成分及含量差异研究[D].北京:中国中医科学院,2015.
[4]陈静,王淑美,孟江,等.一测多评法测定苦参中5种生物碱的含量[J].中国中药杂志,2013,38(9):1406.
[5]涂瑶生,李绍林,孙冬梅,等.星点设计-效应面法优选苦参提取工艺[J].中国实验方剂学杂志,2012,18(5):34.
[6]罗家美.养血祛风止痒法治疗慢性湿疹临床疗效观察[D].广州:广州中医药大学,2010.
[7]曾少峰.湿疹的治法与方剂配伍规律研究[D].南京:南京中医药大学,2007.
[8]郝飞.慢性荨麻疹病因及处理原则[J].皮肤病与性病,2015,37(1):12.
[9]高晓山.中药药性论[M].北京:人民卫生出版社,1992:160.
[10]杨文国,陈军,刘培,等.基于数据挖掘方法研究挥发油透皮促渗作用与中药药性间的关联性[J].中国中药杂志,2015,40(23):4609.
[11]吴玉,陈军,方芸,等.马钱子碱传递体的体外透皮与促渗性质研究[J].中国中药杂志,2016,41(16):3009.
[12]王晖,薛漫清.呋塞米的鼻腔吸收及薄荷醇的促进作用[J].中国临床药理学与治疗学,2000(1):59.
[13]李芸,闫治攀,魏舒畅,等.盐酸小檗碱、吴茱萸挥发油、大黄总蒽醌促进苦参碱透皮吸收的量效关系研究[J].中成药,2014,36(3):510.
[14]周倩,魏玉辉,杨阳,等.槐定碱/苦参碱二元醇脂质体不同制备方法的比较[J].中国医院药学杂志,2013,33(1):39.