两种寄主植物挥发物对梨小食心虫性信息素的增效作用
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摘要
[目的]探索可以增强昆虫对性信息素反应的寄主植物挥发物,提高性信息素的诱捕效果。[方法]使用触角电位技术对寄主植物挥发物顺-3-己烯乙酸酯(A)和乙酸己酯(B)的浓度进行筛选,获得对梨小食心虫雄虫具有最强刺激作用的浓度,随后检测此浓度对性信息素(PH)的增效作用。[结果]A、B 2种物质对雄虫的最强刺激浓度均为100g·L~(-1)。电位值随浓度增加的变化符合Logistic曲线,0.035~2.825g·L~(-1)、0.017~4.592g·L~(-1)分别为电位值快速增加时A、B的浓度区间。A、B分别对PH增效1.6倍、1.4倍,共同增效1.5倍。[结论]A、B 2种物质不论单独或共同,对PH均有明显增效作用,A浓度为100g·L~(-1)时对PH的增效作用最强。
[Objective]To screen host plant volatiles that can increase the efficiency of sex pheromone for monitoring and control of the insect pests.[Methods]We chose two volatiles,cis-3-Hexenyl acetate and hexyl acetate,which were highly abundant in the host plants.First,the optimum concentration to elicit strongest physiological response on antennae of G.molesta males was determined using Electroantennagraphy(EAG).Then,we compared the EAG responses to single sex pheromone and the mixture of sex pheromone with the volatiles at optimum concentration.Logistic curve fitting was used to determine the tendency in the process of concentration screening.[Results]The result showed that,100 g·L~(-1) cis-3-Hexenyl acetate and 100 g·L~(-1) Hexyl acetate each had the strongest stimulating effect for male G.molesta.The increase tendency of EAG values was in accord with Logistic curve.In summary,EAG values increased rapidly when the concentration of cis-3-Hexenyl acetate ranged between 0.035 g·L~(-1)~2.825 g·L~(-1) and the concentration of Hexyl acetate was between 0.017 g·L~(-1)~4.592 g·L~(-1).The antennae response of male G.molesta increased by 1.6,1.4 and 1.5 times,when added 100 g·L~(-1) cis-3-Hexenyl acetate,hexyl acetate and the both into sex pheromone,respectively.[Conclusion]The two host plant volatiles,used alone or in mixture,had significant synergistic effects on sex pheromone.When used at concentration of 100 g·L~(-1),cis-3-Hexenyl acetate showed the highest synergistic effect for sex pheromone.
[Objective]To screen host plant volatiles that can increase the efficiency of sex pheromone for monitoring and control of the insect pests.[Methods]We chose two volatiles,cis-3-Hexenyl acetate and hexyl acetate,which were highly abundant in the host plants.First,the optimum concentration to elicit strongest physiological response on antennae of G.molesta males was determined using Electroantennagraphy(EAG).Then,we compared the EAG responses to single sex pheromone and the mixture of sex pheromone with the volatiles at optimum concentration.Logistic curve fitting was used to determine the tendency in the process of concentration screening.[Results]The result showed that,100 g·L~(-1) cis-3-Hexenyl acetate and 100 g·L~(-1) Hexyl acetate each had the strongest stimulating effect for male G.molesta.The increase tendency of EAG values was in accord with Logistic curve.In summary,EAG values increased rapidly when the concentration of cis-3-Hexenyl acetate ranged between 0.035 g·L~(-1)~2.825 g·L~(-1) and the concentration of Hexyl acetate was between 0.017 g·L~(-1)~4.592 g·L~(-1).The antennae response of male G.molesta increased by 1.6,1.4 and 1.5 times,when added 100 g·L~(-1) cis-3-Hexenyl acetate,hexyl acetate and the both into sex pheromone,respectively.[Conclusion]The two host plant volatiles,used alone or in mixture,had significant synergistic effects on sex pheromone.When used at concentration of 100 g·L~(-1),cis-3-Hexenyl acetate showed the highest synergistic effect for sex pheromone.
引文
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[4]范仁俊,李捷,马春森.北方果树食心虫监测和防控新技术研究与示范[J].山西农业科学,2010,38(5):32-35.
[5]Ilichev A L,Williams D G,Gut L J.Dual pheromone dispenser for combined control of codling moth Cydia pomonella L.and oriental fruit moth Grapholita molesta,(Busck)(Lep.Tortricidae)in pears[J].Journal of Applied Entomology,2007,131(5):368-376.
[6]Ilichev A L,Williams D G,Milner A D.Mating disruption barriers in pome fruit for improved control of oriental fruit moth Grapholita molesta,Busck(Lep.Tortricidae)in stone fruit under mating disruption[J].Journal of Applied Entomology,2004,128(2):126-132.
[7]张利军,陈晓东,帅赛,等.利用性诱剂防治梨小食心虫的研究试验[J].山西农业科学,2010,38(7):97-100.
[8]孟宪佐.我国昆虫信息素研究与应用的进展[J].应用昆虫学报,2000,37(2):75-84.
[9]Reddy G V P,Guerrero A.Interactions of insect pheromones and plant semiochemicals[J].Trends in Plant Science,2004,9(5):253-261.
[10]王振华,赵晖,李金甫,等.植物源挥发物对昆虫信息素的增效作用及其增效机制[J].应用生态学报,2008,19(11):2533-2537.
[11]叶香,韩善捷,韩宝瑜.菜园间作柑桔杨梅和吊瓜对害虫和天敌个数的影响[J].安徽农业大学学报,2016,43(1):6-10.
[12]严善春,张丹丹,迟德富.植物挥发性物质对昆虫作用的研究进展[J].应用生态学报,2003,14(2):310-313.
[13]杜家纬.植物-昆虫间的化学通讯及其行为控制[J].植物生理学报,2001,27(3):193-200.
[14]苏梅,师刘琦,戴截止.优雅粗端叶蝉成虫触角感器的超微形态研究[J].西北农林科技大学学报(自然科学版),2015,43(11):105-112.
[15]黄翠虹,李静静,周琳,等.昆虫触角电位(EAG)及其与气谱联用(GC-EAD)技术[J].应用昆虫学报,2014,51(2):579-585.
[16]崔党群,Logistic曲线方程的解析与拟合优度测验[J].数理统计与管理,2005,24(1):112-115.
[17]Najar-Rodriguez A,Orschel B,Dorn S.Season-long volatile emissions from peach and pear trees in situ,overlapping profiles,and olfactory attraction of an oligophagous fruit moth in the laboratory[J].Journal of Chemical Ecology,2013,39(3):418-29.
[18]朱根海,刘祖祺,朱培仁.应用Logistic方程确定植物组织低温半致死温度的研究[J].南京农业大学学报,1986,9(3):11-16.
[19]冯国双,谭德讲,刘韫宁,等.四参数log-logistic模型在生物活性测定研究中的应用[J].药物分析杂志,2013(11):1849-1851.
[20]Light D M,Flath R A,Buttery R G,et al.Host-plant green-leaf volatiles synergize the synthetic sex pheromones of the corn earworm and codling moth(Lepidoptera)[J].Chemoecology,1993,4(3):145-152.
[21]Reddy G V P,Guerrero A.Behavioral responses of the diamondback moth,Plutella xylostella,to green leaf volatiles of Brassica oleracea subsp.capitata[J].Journal of Agricultural and Food Chemistry,2000,48(12):6025-6029.
[2]Zhao Z,Rong E,Li S C,et al.Research on the practical parameters of sex pheromone traps for the oriental fruit moth[J].Pest Management Science,2013,69(10):1181.
[3]Stelinski L L,Il’Ichev A L,Gut L J.Antennal and behavioral responses of virgin and mated oriental fruit moth(Lepidoptera:Tortricidae)females to their sex pheromone[J].Annals of the Entomological Society of America,2006,99(5):898-904.
[4]范仁俊,李捷,马春森.北方果树食心虫监测和防控新技术研究与示范[J].山西农业科学,2010,38(5):32-35.
[5]Ilichev A L,Williams D G,Gut L J.Dual pheromone dispenser for combined control of codling moth Cydia pomonella L.and oriental fruit moth Grapholita molesta,(Busck)(Lep.Tortricidae)in pears[J].Journal of Applied Entomology,2007,131(5):368-376.
[6]Ilichev A L,Williams D G,Milner A D.Mating disruption barriers in pome fruit for improved control of oriental fruit moth Grapholita molesta,Busck(Lep.Tortricidae)in stone fruit under mating disruption[J].Journal of Applied Entomology,2004,128(2):126-132.
[7]张利军,陈晓东,帅赛,等.利用性诱剂防治梨小食心虫的研究试验[J].山西农业科学,2010,38(7):97-100.
[8]孟宪佐.我国昆虫信息素研究与应用的进展[J].应用昆虫学报,2000,37(2):75-84.
[9]Reddy G V P,Guerrero A.Interactions of insect pheromones and plant semiochemicals[J].Trends in Plant Science,2004,9(5):253-261.
[10]王振华,赵晖,李金甫,等.植物源挥发物对昆虫信息素的增效作用及其增效机制[J].应用生态学报,2008,19(11):2533-2537.
[11]叶香,韩善捷,韩宝瑜.菜园间作柑桔杨梅和吊瓜对害虫和天敌个数的影响[J].安徽农业大学学报,2016,43(1):6-10.
[12]严善春,张丹丹,迟德富.植物挥发性物质对昆虫作用的研究进展[J].应用生态学报,2003,14(2):310-313.
[13]杜家纬.植物-昆虫间的化学通讯及其行为控制[J].植物生理学报,2001,27(3):193-200.
[14]苏梅,师刘琦,戴截止.优雅粗端叶蝉成虫触角感器的超微形态研究[J].西北农林科技大学学报(自然科学版),2015,43(11):105-112.
[15]黄翠虹,李静静,周琳,等.昆虫触角电位(EAG)及其与气谱联用(GC-EAD)技术[J].应用昆虫学报,2014,51(2):579-585.
[16]崔党群,Logistic曲线方程的解析与拟合优度测验[J].数理统计与管理,2005,24(1):112-115.
[17]Najar-Rodriguez A,Orschel B,Dorn S.Season-long volatile emissions from peach and pear trees in situ,overlapping profiles,and olfactory attraction of an oligophagous fruit moth in the laboratory[J].Journal of Chemical Ecology,2013,39(3):418-29.
[18]朱根海,刘祖祺,朱培仁.应用Logistic方程确定植物组织低温半致死温度的研究[J].南京农业大学学报,1986,9(3):11-16.
[19]冯国双,谭德讲,刘韫宁,等.四参数log-logistic模型在生物活性测定研究中的应用[J].药物分析杂志,2013(11):1849-1851.
[20]Light D M,Flath R A,Buttery R G,et al.Host-plant green-leaf volatiles synergize the synthetic sex pheromones of the corn earworm and codling moth(Lepidoptera)[J].Chemoecology,1993,4(3):145-152.
[21]Reddy G V P,Guerrero A.Behavioral responses of the diamondback moth,Plutella xylostella,to green leaf volatiles of Brassica oleracea subsp.capitata[J].Journal of Agricultural and Food Chemistry,2000,48(12):6025-6029.