清肠温中方对慢性溃疡性结肠炎模型小鼠氧化应激作用的影响
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摘要
目的:探究清肠温中方对葡聚糖硫酸钠(dextran sulphate sodium,DSS)诱导慢性溃疡性结肠炎模型小鼠氧化应激作用的影响。方法:90只雄性BALB/c小鼠随机分为6组,除空白组外,其余均自由饮用3%DSS方法复制慢性UC模型。用蒸馏水、清肠温中方高、中、低剂量和美沙拉秦缓释颗粒灌胃干预14 d,记录体质量,ELISA法检测血清和结肠SOD、GSH-px、MDA和结肠MPO,HE染色观察结肠病理,免疫组化染色检测结肠黏膜occludin蛋白表达。结果:模型组小鼠体质量、血清和结肠中SOD、GSH-px含量、结肠黏膜occludin表达水平均明显低于空白组(P<0.05或P<0.01),而MDA和结肠MPO含量、病理评分均高于空白组(P<0.01)。清肠温中方中剂量组和美沙拉秦组小鼠体质量、血清和结肠SOD、GSH-px含量、结肠黏膜occludin表达水平均高于模型组(P<0.05),而MDA和结肠MPO含量、病理评分均低于模型组(P<0.05)。结论:对于DSS诱导的慢性UC模型小鼠,清肠温中方可以促进机体抗氧化、抑制氧化作用,减轻氧化应激反应从而起到治疗作用。
Objective:To study the effect of Qingchang Wenzhong formula(QCWZ) on oxidative stress in chronic ulcerative colitis(UC) model mice induced by dextran sulphate sodium(DSS).Methods:A total of 90 male BALB/c mice were divided into 6 groups randomly,which would drank 3% DSS solution freely for 7 days except blank mice,and distilled water were supplied for another 7 days.One period contained 14 days and 3 periods were used for copying the chronic UC model.Afterwards,the mice were gavaged with corresponding medications for 14 days.The body weights were recorded,meanwhile,SOD,GSH-px and MDA in serum and colonic mucosa,as well as MPO in colons,were observed by enzyme linked immunoassay.Occludin proteins in colons were found by immunohistochemistry and histological score(HS) were observed by HE staining.Results:The model group showed lower body weights,SOD,GSH-px and Occludin proteins than blank group(P<0.05 or P<0.01),while higher MDA,MPO and pathological score than blank group(P<0.01).QCWZ middle dose group and mesalazine group showed higher body weights,SOD,GSH-px and expression of occludin than model group(P<0.05),while lower MDA,MPO and pathological score than model group(P<0.05).Conclusion:QCWZ could promote anti-oxidation,inhibit oxidative effect and decrease oxidative stress to treat chornic UC model mice induced by DSS.
Objective:To study the effect of Qingchang Wenzhong formula(QCWZ) on oxidative stress in chronic ulcerative colitis(UC) model mice induced by dextran sulphate sodium(DSS).Methods:A total of 90 male BALB/c mice were divided into 6 groups randomly,which would drank 3% DSS solution freely for 7 days except blank mice,and distilled water were supplied for another 7 days.One period contained 14 days and 3 periods were used for copying the chronic UC model.Afterwards,the mice were gavaged with corresponding medications for 14 days.The body weights were recorded,meanwhile,SOD,GSH-px and MDA in serum and colonic mucosa,as well as MPO in colons,were observed by enzyme linked immunoassay.Occludin proteins in colons were found by immunohistochemistry and histological score(HS) were observed by HE staining.Results:The model group showed lower body weights,SOD,GSH-px and Occludin proteins than blank group(P<0.05 or P<0.01),while higher MDA,MPO and pathological score than blank group(P<0.01).QCWZ middle dose group and mesalazine group showed higher body weights,SOD,GSH-px and expression of occludin than model group(P<0.05),while lower MDA,MPO and pathological score than model group(P<0.05).Conclusion:QCWZ could promote anti-oxidation,inhibit oxidative effect and decrease oxidative stress to treat chornic UC model mice induced by DSS.
引文
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[11]谢妲.涩肠青黛散联合美沙拉嗪对溃疡性结肠炎患者炎症因子及氧化应激的影响[J].中医学报,2017,32(6):1063-1066.
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[13]鲁蕾,李春涛,张烨.双岐杆菌三联活菌治疗溃疡性结肠炎对炎症因子、氧化应激及T淋巴细胞亚群的影响[J].海南医学院学报,2017,23(16):2192-2195,2199.
[14]马涛.Nrf-2在溃疡性结肠炎病灶组织中的表达及其与抗氧化酶含量、组织损伤程度的相关性[J].海南医学院学报,2017,23(13):1783-1786.
[15]许晨,宇永军,张轶,等.葛根芩连五炭汤对溃疡性结肠炎患者炎症因子和氧化应激的影响[J].中医学报,2017,32(6):1067-1071.
[16]姜爱雯,杜佩珊,汤建华,等.中药有效成分治疗溃疡性结肠炎作用机制的研究进展[J].中医药导报,2017,23(22):109-113.
[17]张苏,王志斌,王跃,等.小檗碱在溃疡性结肠炎中作用及机制的研究进展[J].国际消化病杂志,2016,36(3):136-138,193.
[2]罗雲,岳小强.近十年轻中度溃疡性结肠炎的中医药治疗研究概况[J].中医药导报,2016,22(12):93-97.
[3]Piechota-Polanczyk A,Fichna J.Review article:the role of oxidative stress in pathogenesis and treatment of inflammatory bowel diseases[J].Naunyn Schmiedebergs Arch Pharmacol,2014(387):605-620.
[4]Pedersen J,Coskun M,Soendergaard C,et al.Inflammatory pathways of importance for management of inflammatory bowel disease[J].World J Gastroenterol,2014(20):64-77.
[5]Lianjie Lin,Yan Sun,Dongxu Wang,et al.Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition[J].Front Pharmacol,2015(6):1-14.
[6]Gao W,Guo Y,Wang C,et al.Indirubin ameliorates dextran sulfate sodium-induced ulcerative colitis in mice through the inhibition of inflammation and the induction of Foxp3-expressing regulatory T cells[J].Acta Histochem,2016,118(6):606-614.
[7]Mao TY,Shi R,Zhao W H,et al.Qingchang Wenzhong Decoction Ameliorates Dextran Sulphate Sodium-Induced Ulcerative Colitis in Rats by Downregulating the IP10/CXCR3 Axis-Mediated Inflammatory Response[J].Evid Based Complement Alternat Med,2016(2016):4312538.
[8]张春林.康复新液治疗溃疡性结肠炎小鼠模型的疗效观察及机制初探[D].北京:北京协和医学院,2016:1-61.
[9]Eichele D D,Kharbanda K K.Dextran sodium sulfate colitis murine model:An indispensable tool for advancing our understanding of inflammatory bowel diseases pathogenesis[J].World J Gastroenterol,2017,23(33):6016-6029.
[10]刘利荣,邱克.益气活血解毒方剂配合西药治疗活动期溃疡性结肠炎的疗效及对血管内皮细胞功能及氧化应激的影响[J].现代中西医结合杂志,2017,26(25):2802-2804.
[11]谢妲.涩肠青黛散联合美沙拉嗪对溃疡性结肠炎患者炎症因子及氧化应激的影响[J].中医学报,2017,32(6):1063-1066.
[12]杨佳,范恒.复方苦参汤对溃疡性结肠炎大鼠炎性反应和氧化应激的影响[J].中华中医药杂志,2017,32(8):3457-3461.
[13]鲁蕾,李春涛,张烨.双岐杆菌三联活菌治疗溃疡性结肠炎对炎症因子、氧化应激及T淋巴细胞亚群的影响[J].海南医学院学报,2017,23(16):2192-2195,2199.
[14]马涛.Nrf-2在溃疡性结肠炎病灶组织中的表达及其与抗氧化酶含量、组织损伤程度的相关性[J].海南医学院学报,2017,23(13):1783-1786.
[15]许晨,宇永军,张轶,等.葛根芩连五炭汤对溃疡性结肠炎患者炎症因子和氧化应激的影响[J].中医学报,2017,32(6):1067-1071.
[16]姜爱雯,杜佩珊,汤建华,等.中药有效成分治疗溃疡性结肠炎作用机制的研究进展[J].中医药导报,2017,23(22):109-113.
[17]张苏,王志斌,王跃,等.小檗碱在溃疡性结肠炎中作用及机制的研究进展[J].国际消化病杂志,2016,36(3):136-138,193.