儿童过敏性紫癜血清中IFN-γ、IL-10、TGF-β、PFP、GNLY的表达及意义
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摘要
目的:分析过敏性紫癜(HSP)患儿血清中干扰素-γ(IFN-γ)、白细胞介素10(IL-10)和转化生长因子-β(TGF-β)及穿孔素(PFP)、颗粒溶素(GNLY)的表达水平,并探讨其表达意义。方法:选择本院收治的86例HSP患儿作为研究对象(HSP组),根据是否合并肾损伤分为肾损伤组41例和无肾损伤组45例,同时期40例健康儿童为对照组。采用酶联免疫吸附实验(ELISA)检测受试儿童血清IFN-γ、IL-10和TGF-β表达水平,RT-PCR检测血清PFP、GNLY表达水平,比较不同组之间差异,并分析HSP组血清IFN-γ、IL-10、TGF-β、PFP、GNLY之间的关系。结果:HSP组血清IFN-γ、PFP、GNLY水平低于对照组(P<0.05),血清IL-10、TGF-β水平高于对照组(P<0.05);肾损害组血清IFN-γ、PFP、GNLY水平低于无肾损害组(P<0.05),血清IL-10、TGF-β水平高于无肾损害组(P<0.05);HSP组血清IFN-γ与TGF-β呈负相关关系(r=-0.417,P=0.037),与PFP、GNLY分别呈正相关关系(r=0.508、0.477,P=0.025、0.030);IL-10与TGF-β呈正相关关系(r=0.514,P=0.017)。结论:血清IFN-γ、IL-10、TGF-β、PFP、GNLY水平变化与HSP发生及进展密切相关,早期检测有助于HSP病情评估,对临床预防过敏性紫癜肾炎的发生具有指导意义。
Objective:To investigate the expressions of interferon-γ(IFN-γ),interleukin-10(IL-10),transforming growth factor-β(TGF-β),perforin(PFP)and granulysin(GNLY)in serum of children with Henoch-Schonlein purpura(HSP)and their significances.Methods:A total of 86 children with HSP admitted to our hospital from April 2016 to April2018 were selected as the study subjects(HSP group).According to whether the disease was combined with renal injury or not,the children were divided into kidney injury group(41 cases)and non-kidney injury group(45 cases).Besides,40 healthy children were selected as the control group.The levels of serum IFN-γ,IL-10 and TGF-βwere detected by enzyme-linked immunosorbent assay(ELISA),while levels of serum PFP and GNLY were detected by RT-PCR.The differences between different groups were compared,and the relationship of serum IFN-γ,IL-10,TGF-β,PFP and GNLY in HSP group was also analyzed.Results:The levels of serum IFN-γ,PFP and GNLY in HSP group were lower than those in control group(P<0.05),while the levels of serum IL-10 and TGF-βwere higher than those in control group(P< 0.05);the levels of serum IFN-γ,PFP and GNLY in renal injury group were lower than those in non-renal injury group(P<0.05),while the levels of serum IL-10 and TGF-βwere higher than those in non-renal injury group(P<0.05);in HSP group,serum IFN-γwas negatively correlated with TGF-β(r=-0.417,P=0.037),and positively correlated with PFP and GNLY(r=0.508,0.477,P=0.025,0.030);there was a positive correlation between IL-10 and TGF-β(r=0.514,P=0.017).Conclusion:The changes on the levels of serum IFN-γ,IL-10,TGF-β,PFP and GNLY are closely related to the occurrence and progression of HSP,early detection is helpful to the evaluation of HSP and has guiding significance for clinical prevention of Henoch-Schonlein purpura nephritis.
Objective:To investigate the expressions of interferon-γ(IFN-γ),interleukin-10(IL-10),transforming growth factor-β(TGF-β),perforin(PFP)and granulysin(GNLY)in serum of children with Henoch-Schonlein purpura(HSP)and their significances.Methods:A total of 86 children with HSP admitted to our hospital from April 2016 to April2018 were selected as the study subjects(HSP group).According to whether the disease was combined with renal injury or not,the children were divided into kidney injury group(41 cases)and non-kidney injury group(45 cases).Besides,40 healthy children were selected as the control group.The levels of serum IFN-γ,IL-10 and TGF-βwere detected by enzyme-linked immunosorbent assay(ELISA),while levels of serum PFP and GNLY were detected by RT-PCR.The differences between different groups were compared,and the relationship of serum IFN-γ,IL-10,TGF-β,PFP and GNLY in HSP group was also analyzed.Results:The levels of serum IFN-γ,PFP and GNLY in HSP group were lower than those in control group(P<0.05),while the levels of serum IL-10 and TGF-βwere higher than those in control group(P< 0.05);the levels of serum IFN-γ,PFP and GNLY in renal injury group were lower than those in non-renal injury group(P<0.05),while the levels of serum IL-10 and TGF-βwere higher than those in non-renal injury group(P<0.05);in HSP group,serum IFN-γwas negatively correlated with TGF-β(r=-0.417,P=0.037),and positively correlated with PFP and GNLY(r=0.508,0.477,P=0.025,0.030);there was a positive correlation between IL-10 and TGF-β(r=0.514,P=0.017).Conclusion:The changes on the levels of serum IFN-γ,IL-10,TGF-β,PFP and GNLY are closely related to the occurrence and progression of HSP,early detection is helpful to the evaluation of HSP and has guiding significance for clinical prevention of Henoch-Schonlein purpura nephritis.
引文
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13 李怡静,靳颜超,王松江,等.过敏性紫癜患儿急性期血清IL-10、IL-13、IL-15水平变化与意义[J].河南大学学报(医学版),2016,35(2):117-118.
14 谭雄俊,杨宝辉,张丽,等.IL-10+B细胞对儿童过敏性紫癜Th17/Treg免疫平衡紊乱的影响[J].免疫学杂志,2017,33(2):133-140.
15 杨新凤,王会荣,田浩,等.过敏性紫癜患儿血清IL-21,TGF-β1,TNF-α及IgAl水平与紫癜性肾炎发生的相关性研究术[J].现代生物医学进展,2017,17(22):4295-4298.
16 邢静,李文斌,王艳飞,等.儿童过敏性紫癜血清IL-21、TGF-β1、TNF-α、IgA1的变化与紫癜性肾炎发生的相关性研究[J].基因组学与应用生物学,2017,10(7):2633-2639.
17 刘佳柏,翟利云,李秀英,等.颗粒溶素的研究进展[J].现代生物医学进展,2014,14(20):3957-3959.
18 Prévost‐Blondel A,Neuenhahn M,Rawiel M,et al.Differential requirement of perforin and IFN‐γin CD8T cell‐mediated immune responses against B16.F10melanoma cells expressing a viral antigen[J].European Journal of Immunology,2015,30(9):2507-2515.
2 唐筱潇,李婷,陈学兰,等.滤泡辅助性T细胞及亚群紊乱参与儿童过敏性紫癜发病机制初探[J].免疫学杂志,2018,34(2):120-127.
3 胡亚美江载芳.诸福棠实用儿科学(上下)(精)[M].7版.北京:人民卫生出版社,2012:408-415.
4 张建江,史佩佩,王淼,等.Th17/Treg失衡及IL-17、TGF-β1水平在儿童过敏性紫癜和紫癜性肾炎中的研究[J].中华肾脏病杂志,2014,30(9):702-703.
5 Natsuko SS,Yu S,Shun O,et al.Anaphylactoid purpura triggered by cellulitis as a favorable prognosis:case report and literature review[J].Springerplus,2016,5(1):1112.
6 刘德滢,鲁莹,刘敏,等.过敏性紫癜的发病机制及糖皮质激素在治疗中的地位[J].中华风湿病学杂志,2014,18(9):640-642.
7 Kamei K,Ogura M,Mai S,et al.Evolution of IgA nephropathy into anaphylactoid purpura in six cases—further evidence that IgA nephropathy and Henoch–Schonlein purpura nephritis share common pathogenesis[J].Pediatric Nephrology,2016,31(5):779-785.
8 吴容,王强,董巍.过敏性紫癜患儿血浆IL-2、IFN-γ、IL-4与TGF-β1的变化及其意义[J].实用医院临床杂志,2014,11(1):144-146.
9 张自力,王高峰,梅道启,等.过敏性紫癜患儿外周血单核细胞TLR2表达及与免疫应答的相关性[J].中国组织工程研究,2015,19(45):7356-7361.
10 Gregori S,Tomasoni D,Pacciani V,et al.Differentiation of type 1Tregulatory cells(Tr1)by tolerogenic DC-10 requires the IL-10-dependent ILT4/HLA-G pathway[J].Blood,2016,116(6):935-944.
11 Yuan P,Bo Y,Ming G,et al.Apoptosis of human umbilical vein endothelial cells(HUVEC)induced by IgA1isolated from Henoch-Schonlein purpura children[J].Asian Pac J Allergy Immunol,2014,32(1):34-38.
12 王伟,董萍云,寇晓丽,等.过敏性紫癜患者血清IL-10,IL-26水平的变化及其意义[J].中国皮肤性病学杂志,2014,28(7):690-692.
13 李怡静,靳颜超,王松江,等.过敏性紫癜患儿急性期血清IL-10、IL-13、IL-15水平变化与意义[J].河南大学学报(医学版),2016,35(2):117-118.
14 谭雄俊,杨宝辉,张丽,等.IL-10+B细胞对儿童过敏性紫癜Th17/Treg免疫平衡紊乱的影响[J].免疫学杂志,2017,33(2):133-140.
15 杨新凤,王会荣,田浩,等.过敏性紫癜患儿血清IL-21,TGF-β1,TNF-α及IgAl水平与紫癜性肾炎发生的相关性研究术[J].现代生物医学进展,2017,17(22):4295-4298.
16 邢静,李文斌,王艳飞,等.儿童过敏性紫癜血清IL-21、TGF-β1、TNF-α、IgA1的变化与紫癜性肾炎发生的相关性研究[J].基因组学与应用生物学,2017,10(7):2633-2639.
17 刘佳柏,翟利云,李秀英,等.颗粒溶素的研究进展[J].现代生物医学进展,2014,14(20):3957-3959.
18 Prévost‐Blondel A,Neuenhahn M,Rawiel M,et al.Differential requirement of perforin and IFN‐γin CD8T cell‐mediated immune responses against B16.F10melanoma cells expressing a viral antigen[J].European Journal of Immunology,2015,30(9):2507-2515.