杜仲-牛膝药对干预去卵巢骨质疏松大鼠雌二醇和骨密度的影响实验研究
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摘要
目的观察杜仲-牛膝配伍对卵巢切除所致实验性骨质疏松大鼠的血清雌二醇(E2)和股骨骨密度(BMD)的影响,探讨杜仲-牛膝药对防治绝经后骨质疏松(PMOP)的作用机制。方法取72只雌性SD大鼠随机分为假手术组、模型组、阳性对照组、杜仲组、牛膝组、杜仲-牛膝组共6组,每组12只。行卵巢切除术造模,其中假手术组只切除卵巢周围少量脂肪。造模成功后正常饲养7 d后开始给药,连续给药3个月后取腹主动脉血测定E2,并分离左侧股骨,检测BMD。结果与模型组比较,各给药组E2、BMD均显著增高(P<0.05),且杜仲-牛膝组E2水平显著高于杜仲组、牛膝组。结论杜仲-牛膝药对可显著提高去卵巢骨质疏松大鼠E2水平和股骨BMD,显示了其对PMOP的治疗效果,也为开发治疗PMOP的中药复方提供了依据。
Objective To determine the effect of eucommia ulmoldes and achyranthes combination on the serum estrogen and femoral bone mineral density in ovariectomy induced osteoporosis rats, and to explore the therapeutic mechanism of eucommia ulmoldes and achyranthes on postmenopausal osteoporosis(PMOP). Methods Totally 72 female SD rats were divided into 6 groups 12 in each: a sham operation group, a model group, a positive control group, a eucommia ulmoldes group, an achyranthes group and a eucommia ulmoldes coupled with achyranthes group. A small amount of fat was cut in rats in the sham operation group around the ovary excision. Other groups were ovariectomized. After success modeling, the rats were fed normally intragastrically. They were administratered a total of 3 months. Blood was drawn from ventral aorta and the left side femur head isolated in each rat to determine serum estradiol and bone mineral density. Results Compared with the model group, the serum estradiol level and bone mineral density of each medication group were both increased significantly(P < 0.05). The serum estradiol level of eucommia ulmoldes coupled with achyranthes group was significantly higher than that of eucommia ulmoldes group and achyranthes group. Conclusion The combination of eucommia ulmoldes and achyranthes can significantly increase the serum estradiol level and bone mineral density in PMOP rats, which indicates that they have good therapeutic effect on PMOP. This research provides an experimental reference for the development of Chinese medicine for PMOP.
Objective To determine the effect of eucommia ulmoldes and achyranthes combination on the serum estrogen and femoral bone mineral density in ovariectomy induced osteoporosis rats, and to explore the therapeutic mechanism of eucommia ulmoldes and achyranthes on postmenopausal osteoporosis(PMOP). Methods Totally 72 female SD rats were divided into 6 groups 12 in each: a sham operation group, a model group, a positive control group, a eucommia ulmoldes group, an achyranthes group and a eucommia ulmoldes coupled with achyranthes group. A small amount of fat was cut in rats in the sham operation group around the ovary excision. Other groups were ovariectomized. After success modeling, the rats were fed normally intragastrically. They were administratered a total of 3 months. Blood was drawn from ventral aorta and the left side femur head isolated in each rat to determine serum estradiol and bone mineral density. Results Compared with the model group, the serum estradiol level and bone mineral density of each medication group were both increased significantly(P < 0.05). The serum estradiol level of eucommia ulmoldes coupled with achyranthes group was significantly higher than that of eucommia ulmoldes group and achyranthes group. Conclusion The combination of eucommia ulmoldes and achyranthes can significantly increase the serum estradiol level and bone mineral density in PMOP rats, which indicates that they have good therapeutic effect on PMOP. This research provides an experimental reference for the development of Chinese medicine for PMOP.
引文
[1]杨通宇,胡建山,李文忠,等.鹿角壮骨胶囊治疗绝经后骨质疏松症患者的临床疗效观察[J].中国妇幼保健,2016,31(5):1034-1036.
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[17]任心慈,徐先详,许杜鹃,等.牛膝总皂苷对维甲酸致骨质疏松大鼠骨代谢的影响[J].中国实验方剂学杂志,2011,17(4):128-130.
[18]任心慈.牛膝总皂苷防治实验性骨质疏松作用研究[J].安徽医药,2010,14(10):1133-1134.
[19]杨柳,张颖,刘季田媛,等.牛膝补肾壮骨有效部位抗炎、镇痛作用研究[J].中医药学报,2015,43(6):25-28.
[20]高昌琨.怀牛膝对维甲酸所致大鼠骨质疏松防治作用的实验研究[J].基层中药杂志,2001,15(2):9-11.
[21]唐俊峰,路亚娥,胡锡琴.乌牛膝方加味治疗老年人骨质疏松症30例[J].现代中医药,2011,31(4):24-25.
[2]马剑侠,薛鹏,王燕,等.绝经后女性2-型糖尿病患者血脂、血压与骨质疏松的关系[J].中国老年学杂志,2016,36(3):1074-1076.
[3]梁建峰,韩会霞.绝经后女性非酒精性脂肪肝脂代谢紊乱与骨质疏松的相关性[J].河南科技大学学报:医学版,2016,34(1):41-43.
[4]龙梓,刘丹霞,廖华卫,等.健骨片对去卵巢骨质疏松大鼠骨代谢、骨微结构及骨强度的影响[J].中国实验方剂学杂志,2016,22(9):116-121.
[5]王娟,褚小刚.全杜仲胶囊联合红花逍遥片治疗绝经后骨质疏松38例[J].中国老年学杂志,2014,34(2):1086-1087.
[6]富灵杰,汤亭亭,戴尅戎.骨质疏松动物模型的建立和应用[J].中华实验外科杂志,2008,25(2):270-272.
[7]曹春雨,刘婷,回连强,等.黔岭淫羊藿总黄酮类成分对去势雌性大鼠骨质疏松症的实验研究[J].中国中药杂志,2014,39(10):1913-1917.
[8]章元沛.药理学实验[M].第2版.北京:人民卫出版社.1996:238.
[9]金珉廷,郑洪新.中医肾藏精生髓主骨理论与骨质疏松症[J].辽宁中医药大学学报,2009,11(3):35.
[10]马俊岭,郭海英,侯钦午.绝经后骨质疏松症的病因病理[J].淮海医药,2010,28(5):3-4.
[11]谢雁鸣,宇文亚.原发性骨质疏松症中医临床实践指南(摘录)[J].中华中医药杂志,2012,27(7):1886-1890.
[12]赖卫国,李韶今,钟叙春.仙灵脾散联合热敏灸治疗老年性骨质疏松症疼痛的疗效[J].实用中西医结合临床,2010,10(4):54-55.
[13]段卫华,牛彦兵,崔茗婉,等.杜仲丸不同配比对去卵巢大鼠骨质疏松症的影响[J].中国实验方剂学杂志,2016,22(7):130-133.
[14]白立炜.杜仲叶醇提物预防去势大鼠骨质疏松症的实验研究[J].中国民康医学,2008,20(15):1715-1717.
[15]葛文杰.杜仲对去势雌性骨质疏松大鼠骨代谢、骨生物力学的影响[J].山东中医药大学学报,2009,33(5):417-419.
[16]陶弘景,尚志钧.名医别录[M].北京:中国中医药出版,2013:8-29.
[17]任心慈,徐先详,许杜鹃,等.牛膝总皂苷对维甲酸致骨质疏松大鼠骨代谢的影响[J].中国实验方剂学杂志,2011,17(4):128-130.
[18]任心慈.牛膝总皂苷防治实验性骨质疏松作用研究[J].安徽医药,2010,14(10):1133-1134.
[19]杨柳,张颖,刘季田媛,等.牛膝补肾壮骨有效部位抗炎、镇痛作用研究[J].中医药学报,2015,43(6):25-28.
[20]高昌琨.怀牛膝对维甲酸所致大鼠骨质疏松防治作用的实验研究[J].基层中药杂志,2001,15(2):9-11.
[21]唐俊峰,路亚娥,胡锡琴.乌牛膝方加味治疗老年人骨质疏松症30例[J].现代中医药,2011,31(4):24-25.