左归丸联合温和灸对骨质疏松症模型大鼠骨密度、骨保护素mRNA及核因子κB受体活化因子配体mRNA的影响
|
摘要
目的探讨左归丸联合温和灸治疗骨质疏松症的可能作用机制。方法 40只雌性SD大鼠随机分为空白组、模型组、左归丸组、左归丸+温和灸组,每组10只。模型组、左归丸组、左归丸+温和灸组采用手术完整摘除双侧卵巢法制造骨质疏松症模型。造模3个月后左归丸组予左归丸10 ml/(kg·d)灌胃,左归丸+温和灸组予左归丸10 ml/(kg·d)灌胃+长强穴艾灸治疗,空白组和模型组予生理盐水10 ml/(kg·d)灌胃。各组均干预6周。干预前后测定大鼠股骨近端及第2腰椎骨密度,干预后通过PCR法检测大鼠骨组织骨保护素(OPG)mRNA和核因子κB受体活化因子配体(RANKL)mRNA表达水平。结果与空白组比较,模型组股骨、腰椎骨密度,骨组织OPG mRNA表达及OPG/RANKL降低,RANKL mRNA表达上升(P<0.05或P<0.01);与模型组比较,左归丸组、左归丸+温和灸组大鼠股骨、腰椎骨密度,骨组织OPG mRNA表达及OPG/RANKL均升高,RANKL mRNA表达降低(P<0.05或P<0.01),且左归丸+温和灸组大鼠股骨、腰椎骨密度,骨组织OPG mRNA表达及OPG/RANKL均较左归丸组升高,RANKL mRNA表达较左归丸组降低(P<0.05)。结论左归丸联合温和灸能明显升高骨质疏松症大鼠的骨密度,上调OPG mRNA表达、下调RANKL mRNA表达可能是其作用机制之一。
Objective To explore the possible mechanism of Zuogui Wan( 左归丸) combined with mild-warm moxibustion in treating osteoporosis. Methods Forty female SD rats were randomized into blank group,model group,Zuogui Wan group and Zuogui Wan + mild-warm moxibustion group,10 rats in each group. Rats in the model group,Zuogui Wan group and Zuogui Wan + mild-warm moxibustion group were made osteoporosis models by completely removing bilateral ovaries. Three months after modeling,the Zuogui Wan group was given Zuogui Wan 10ml/( kg·d) by gavage. The Zuogui Wan + mild-warm moxibustion group was given Zuogui Wan 10 ml/( kg·d) by gavage and moxibustion at " Changqiang( DU1) "point. The blank group and the model group were given normal saline 10 ml/( kg·d) by gavage. All groups were treated for 6 weeks. Rats' bone mineral density of proximal femur and the second lumbar vertebra were measured before and after treatment. After treatment,mRNA expression levels of rats bone tissue osteoprotegerin( OPG) and nuclear factor κB receptor activator ligand( RANKL) were detected by polymerase chain reaction( PCR) method. Results Compared with the blank group,bone mineral density of femur and lumbar vertebra,bone tissue OPG mRNA expression,as well as OPG/RANKL in the model group decreased;while RANKL mRNA expression increased( P < 0. 05 or P < 0. 01). Compared with the model group,rats' bone mineral density of femur and lumbar vertebra,bone tissue OPG mRNA expression,as well as OPG/RANKL in the Zuogui Wan group and the Zuogui Wan + mild-warm moxibustion group increased; while RANKL mRNA expression decreased( P < 0. 05 or P < 0. 01). Moreover,rats' bone mineral density of femur and lumbar vertebra,bone tissue OPG mRNA expression,as well as OPG/RANKL in the Zuogui Wan + mild-warm moxibustion group were higher than those in the Zuogui Wan group; while RANKL mRNA expression was lower( P < 0. 05). Conclusion Zuogui Wan combined with mild-warm moxibustion might increase osteoporosis rats' bone mineral density. Up-regulating OPG mRNA expression and down-regulating RANKL mRNA expression seem to be one of the mechanisms.
Objective To explore the possible mechanism of Zuogui Wan( 左归丸) combined with mild-warm moxibustion in treating osteoporosis. Methods Forty female SD rats were randomized into blank group,model group,Zuogui Wan group and Zuogui Wan + mild-warm moxibustion group,10 rats in each group. Rats in the model group,Zuogui Wan group and Zuogui Wan + mild-warm moxibustion group were made osteoporosis models by completely removing bilateral ovaries. Three months after modeling,the Zuogui Wan group was given Zuogui Wan 10ml/( kg·d) by gavage. The Zuogui Wan + mild-warm moxibustion group was given Zuogui Wan 10 ml/( kg·d) by gavage and moxibustion at " Changqiang( DU1) "point. The blank group and the model group were given normal saline 10 ml/( kg·d) by gavage. All groups were treated for 6 weeks. Rats' bone mineral density of proximal femur and the second lumbar vertebra were measured before and after treatment. After treatment,mRNA expression levels of rats bone tissue osteoprotegerin( OPG) and nuclear factor κB receptor activator ligand( RANKL) were detected by polymerase chain reaction( PCR) method. Results Compared with the blank group,bone mineral density of femur and lumbar vertebra,bone tissue OPG mRNA expression,as well as OPG/RANKL in the model group decreased;while RANKL mRNA expression increased( P < 0. 05 or P < 0. 01). Compared with the model group,rats' bone mineral density of femur and lumbar vertebra,bone tissue OPG mRNA expression,as well as OPG/RANKL in the Zuogui Wan group and the Zuogui Wan + mild-warm moxibustion group increased; while RANKL mRNA expression decreased( P < 0. 05 or P < 0. 01). Moreover,rats' bone mineral density of femur and lumbar vertebra,bone tissue OPG mRNA expression,as well as OPG/RANKL in the Zuogui Wan + mild-warm moxibustion group were higher than those in the Zuogui Wan group; while RANKL mRNA expression was lower( P < 0. 05). Conclusion Zuogui Wan combined with mild-warm moxibustion might increase osteoporosis rats' bone mineral density. Up-regulating OPG mRNA expression and down-regulating RANKL mRNA expression seem to be one of the mechanisms.
引文
[1]IVANOVA S,VASILEVA L,IVANOVA S,et al.Osteoporosis:therapeutic options[J].Folia Med(Plovdiv),2015,57(3-4):181-190.
[2]CHEN SJ,CHEN YJ,CHENG CH,et al.Comparisons of different screening tools for identifying fracture/osteoporosis risk among community-dwelling older people[J].Medicine(Baltimore),2016,95(20):e3415.doi:10.1097/MD.0000000000003415.
[3]PRINCE M,GLUECK CJ,SHAH P,et al.Hospitalization for pulmonary embolism associated with antecedent testosterone or estrogen therapy in patients found to have familial and acquired thrombophilia[J].BMC Hematol,2016,8(16):6-13.
[4]夏炳江,童培建,孙燕,等.骨质疏松肾阴虚型小鼠病证结合模型建立的实验研究[J].中国中医急症,2013,22(7):1083-1086.
[5]欧阳建江,刘庆思,许辛寅,等.温和灸法对原发性骨质疏松症患者骨痛视觉模拟评分及血清骨保护素的影响[J].中国康复医学杂志,2012,27(10):971-972.
[6]富灵杰,汤亭亭,戴尅戎.骨质疏松动物模型的建立和应用[J].中华实验外科杂志,2008,25(2):270-272.
[7]李晶晶,唐苾芯,黄流波,等.中医药对围绝经期综合征妇女下丘脑-垂体-性腺轴影响的研究进展[J].上海中医药杂志,2013,47(12):90-92.
[8]NOLLET M,SANTUCCI-DARMANIN S,BREUIL V,et al.Autophagy in osteoblasts is involved in mineralization and bone homeostasis[J].Autophagy,2014,10(11):1965-1977.
[9]STUSS M,RIESKE P,CEGOWSKA A,et al.Assessment of OPG/RANK/RANKL gene expression levels in peripheral blood mononuclear cells(PBMC)after treatment with strontium ranelate and ibandronate in patients with postmenopausal osteoporosis[J].J Clin Endocrinol Metab,2013,98(5):E1007-E1011.
[10]BORD S,IRELAND DC,BEAVAN SR,et al.The effects of estrogen on osteoprotegerin,RANKL,and estrogen receptor expression in human osteoblasts[J].Bone,2003,32(2):136-141.
[11]仲蕾蕾,杨冰,黄晓,等.OPG/RANKL/RANK系统在成骨细胞和破骨细胞相互调节中的作用[J].中国骨质疏松杂志,2011,17(11):1010-1012.
[12]商敏.OPG/RANKL/RANK基因多态性与绝经后骨质疏松[J].中国骨质疏松杂志,2009,11(1):68-71.
[13]贾少杰,贾晓静.雌激素抗骨质疏松的OPG/RANKL和Apo E作用机制的实验研究[J].中国老年学杂志,2007,27(23):2274-2276.
[2]CHEN SJ,CHEN YJ,CHENG CH,et al.Comparisons of different screening tools for identifying fracture/osteoporosis risk among community-dwelling older people[J].Medicine(Baltimore),2016,95(20):e3415.doi:10.1097/MD.0000000000003415.
[3]PRINCE M,GLUECK CJ,SHAH P,et al.Hospitalization for pulmonary embolism associated with antecedent testosterone or estrogen therapy in patients found to have familial and acquired thrombophilia[J].BMC Hematol,2016,8(16):6-13.
[4]夏炳江,童培建,孙燕,等.骨质疏松肾阴虚型小鼠病证结合模型建立的实验研究[J].中国中医急症,2013,22(7):1083-1086.
[5]欧阳建江,刘庆思,许辛寅,等.温和灸法对原发性骨质疏松症患者骨痛视觉模拟评分及血清骨保护素的影响[J].中国康复医学杂志,2012,27(10):971-972.
[6]富灵杰,汤亭亭,戴尅戎.骨质疏松动物模型的建立和应用[J].中华实验外科杂志,2008,25(2):270-272.
[7]李晶晶,唐苾芯,黄流波,等.中医药对围绝经期综合征妇女下丘脑-垂体-性腺轴影响的研究进展[J].上海中医药杂志,2013,47(12):90-92.
[8]NOLLET M,SANTUCCI-DARMANIN S,BREUIL V,et al.Autophagy in osteoblasts is involved in mineralization and bone homeostasis[J].Autophagy,2014,10(11):1965-1977.
[9]STUSS M,RIESKE P,CEGOWSKA A,et al.Assessment of OPG/RANK/RANKL gene expression levels in peripheral blood mononuclear cells(PBMC)after treatment with strontium ranelate and ibandronate in patients with postmenopausal osteoporosis[J].J Clin Endocrinol Metab,2013,98(5):E1007-E1011.
[10]BORD S,IRELAND DC,BEAVAN SR,et al.The effects of estrogen on osteoprotegerin,RANKL,and estrogen receptor expression in human osteoblasts[J].Bone,2003,32(2):136-141.
[11]仲蕾蕾,杨冰,黄晓,等.OPG/RANKL/RANK系统在成骨细胞和破骨细胞相互调节中的作用[J].中国骨质疏松杂志,2011,17(11):1010-1012.
[12]商敏.OPG/RANKL/RANK基因多态性与绝经后骨质疏松[J].中国骨质疏松杂志,2009,11(1):68-71.
[13]贾少杰,贾晓静.雌激素抗骨质疏松的OPG/RANKL和Apo E作用机制的实验研究[J].中国老年学杂志,2007,27(23):2274-2276.