补肾活血颗粒对去势大鼠肾虚证的影响及机制研究
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摘要
目的:探讨补肾活血颗粒对去势大鼠的肾虚症状的改善作用及作用机制,为临床防治骨质疏松症采用补肾治本的原则提供理论依据。方法:24只雌性大鼠单纯随机分为补肾活血组、模型组、假手术组,每组8只。补肾活血组与模型组麻醉后行双侧卵巢切除术,假手术组麻醉后行假手术处理,饲养3个月确认造模成功后各组分别给予补肾活血颗粒、生理盐水、生理盐水灌胃,90 d后进行3 d大鼠的一般情况观察及生理状态的量化检测,然后麻醉下处死大鼠,用电子天平称取垂体、肾、肾上腺、子宫的湿重,采用Elisa法进行ACTH、E2、T3、T4的血清学检测。结果:补肾活血组大鼠的体毛光滑略显稀疏、行动活泼、反应尚可、精神尚佳、食欲可、身体肥胖、略有嗜睡、眼睛明亮、眼角干燥、二便基本正常等表现;活动度、肛温及进食量均高于模型组,存在显著性差异(P<0.01),与假手术组无明显差异(P>0.05);血压与模型组及假手术组比较无明显差异(P>0.05);血清ACTH、E2、T3、T4的水平明显高于模型组,具有显著性差异(P<0.01),与假手术组无明显差异(P>0.05);垂体、肾上腺、肾的湿重均明显高于模型组,存在显著性差异(P<0.01),与假手术组比较无统计学意义(P>0.05),但子宫的湿重高于模型组,低于假手术组,均存在显著性差异(P<0.05)。结论:补肾活血颗粒能够明显的改善去势大鼠的肾虚症状,并且推测调节下丘脑-垂体-靶腺轴是补肾活血颗粒改善去势大鼠肾虚症状的作用机制。
Objective: To observe kidney deficiency symptoms and the mechanism of ovariectomized rats treated with Bushen Huoxue Particles to provide a theoretical basis for clinical prevention and treatment of osteoporosis. Methods: 24 female SD rats were randomly divided into 3 groups: Bushen Huoxue Particles group,model group and sham group. Rats of Bushen Huoxue Particles group and model group were administrated with ovariectomy for establishing models of postmenopausal osteoporosis,while ovarian of sham group rats were exposed but were not removed. 3 groups were gavaged Bushen Huoxue Particles and saline for 3 months after confirming the model successfully. The general conditions were observed and physiological condition,estrogen( E2),adrenocorticotropic hormone( ACTH),three typical thyroid original acid( T3),four typical thyroid original acid( T4) and weight of kidney,adrenal gland,pituitary and uterus were detected in each group. Results: The general conditions of Bushen Huoxue Particles group was normal,for example,slightly sparse body hair with burnish,lively action,reaction agile,good spirit and appetite,obesity slight drowsiness,bright and dry eyes,normal defecation and urine. The activity,rectal temperature and eating were higher much more than that of the model group and there was statistical difference among these groups( P < 0. 01) but there was no significant difference compared with the sham group( P > 0. 05). The serum levels of E2,ACTH,T3 and T4 levels were significantly higher than those of the model group and there was statistical difference among these groups( P < 0. 01) but there was no significant difference compared with the sham group( P > 0. 05). The weights of pituitary,kidney and adrenal gland were significantly higher than those of the model group and there was statistical difference between these groups( P < 0. 01) but there was no significant difference compared with the sham group( P > 0. 05). The uterus was higher than that in the model group and lower than that in the sham group and there was difference among these groups( P < 0. 05). Conclusion: Bushen Huoxue Particles can significantly improve the kidney deficiency syndrome of the ovariectomized rats. We can speculate that the mechanism of Bushen Huoxue Particles improving the kidney empty symptoms of osteoporosis was the regulation of hypothalamic-pituitary-target gland axis.
Objective: To observe kidney deficiency symptoms and the mechanism of ovariectomized rats treated with Bushen Huoxue Particles to provide a theoretical basis for clinical prevention and treatment of osteoporosis. Methods: 24 female SD rats were randomly divided into 3 groups: Bushen Huoxue Particles group,model group and sham group. Rats of Bushen Huoxue Particles group and model group were administrated with ovariectomy for establishing models of postmenopausal osteoporosis,while ovarian of sham group rats were exposed but were not removed. 3 groups were gavaged Bushen Huoxue Particles and saline for 3 months after confirming the model successfully. The general conditions were observed and physiological condition,estrogen( E2),adrenocorticotropic hormone( ACTH),three typical thyroid original acid( T3),four typical thyroid original acid( T4) and weight of kidney,adrenal gland,pituitary and uterus were detected in each group. Results: The general conditions of Bushen Huoxue Particles group was normal,for example,slightly sparse body hair with burnish,lively action,reaction agile,good spirit and appetite,obesity slight drowsiness,bright and dry eyes,normal defecation and urine. The activity,rectal temperature and eating were higher much more than that of the model group and there was statistical difference among these groups( P < 0. 01) but there was no significant difference compared with the sham group( P > 0. 05). The serum levels of E2,ACTH,T3 and T4 levels were significantly higher than those of the model group and there was statistical difference among these groups( P < 0. 01) but there was no significant difference compared with the sham group( P > 0. 05). The weights of pituitary,kidney and adrenal gland were significantly higher than those of the model group and there was statistical difference between these groups( P < 0. 01) but there was no significant difference compared with the sham group( P > 0. 05). The uterus was higher than that in the model group and lower than that in the sham group and there was difference among these groups( P < 0. 05). Conclusion: Bushen Huoxue Particles can significantly improve the kidney deficiency syndrome of the ovariectomized rats. We can speculate that the mechanism of Bushen Huoxue Particles improving the kidney empty symptoms of osteoporosis was the regulation of hypothalamic-pituitary-target gland axis.
引文
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[6]徐祖健,汪付,尹思源,等.绝经后妇女原发性骨质疏松症辨证分型与症候特征的探讨[J].中医正骨,2008,20(11):1-2.
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[8]李恩,薛延,王洪复,等.骨质疏松鉴别诊断与治疗[M].北京:人民卫生出版社,2005:4.
[9]陈英华,欧阳轶强,孙琪,等.肾阳虚证动物模型规范化研究中诊断指标选择的初步探讨[J].中国中医基础医学杂志,2003,9(10):26-30.
[10]林燕萍,李咏高,王和鸣,等.健骨颗粒对骨质疏松模鼠垂体—甲状腺轴的影响[J].中国骨伤,2002,15(3):154-156.
[11]李广曦.肾阳虚证动物模型的造模方法及其相关指标回顾[J].中国中医基础医学杂志,2000,6(4):256-263.
[12]Yu J.The kidneys and life network in the study of reproductive enlightenment[J].Chinese Journal of Integrative Medicine,2000,20(6):409.
[13]FAN Chao-ling,GE Ji-rong.Research progress of the effect of kidney-tonifying herbs on the related signal transduction pathways of osteoporosis[J].Chin J Osteoporos,2014,20(4):446-451.
[14]富灵杰,汤亭亭,戴尅戎.骨质疏松动物模型的建立和应用[J].中华实验外科杂志,2008,25(2):270-272.
[2]刘慧,许兵,方剑利,等.补肾活血颗粒对去势大鼠血生化的影响[J].中华中医药杂志,2012,27(5):1435-1438.
[3]许兵,金红婷,刘慧,等.补肾活血颗粒对去势大鼠骨组织Wnt/β-Catenin通路的影响研究[J].中华中医药杂志,2013,28(11):3400-3405.
[4]Yu J.Association of osteoporosis and bone mineral salt branch primary osteoporosis disease diagnosis and treatment guidelines[J].Chin J Osteoporosis&Bone Miner Res,2011(4):2-17.
[5]金珉廷,郑洪新.中医肾藏精生髓主骨理论与骨质疏松症[J].辽宁中医药大学学报,2009,11(3):35-36.
[6]徐祖健,汪付,尹思源,等.绝经后妇女原发性骨质疏松症辨证分型与症候特征的探讨[J].中医正骨,2008,20(11):1-2.
[7]许兵,刘慧,金红婷,等.经典骨质疏松症模型大鼠的肾虚证研究[J].中国骨伤,2012,25(9):766-770.
[8]李恩,薛延,王洪复,等.骨质疏松鉴别诊断与治疗[M].北京:人民卫生出版社,2005:4.
[9]陈英华,欧阳轶强,孙琪,等.肾阳虚证动物模型规范化研究中诊断指标选择的初步探讨[J].中国中医基础医学杂志,2003,9(10):26-30.
[10]林燕萍,李咏高,王和鸣,等.健骨颗粒对骨质疏松模鼠垂体—甲状腺轴的影响[J].中国骨伤,2002,15(3):154-156.
[11]李广曦.肾阳虚证动物模型的造模方法及其相关指标回顾[J].中国中医基础医学杂志,2000,6(4):256-263.
[12]Yu J.The kidneys and life network in the study of reproductive enlightenment[J].Chinese Journal of Integrative Medicine,2000,20(6):409.
[13]FAN Chao-ling,GE Ji-rong.Research progress of the effect of kidney-tonifying herbs on the related signal transduction pathways of osteoporosis[J].Chin J Osteoporos,2014,20(4):446-451.
[14]富灵杰,汤亭亭,戴尅戎.骨质疏松动物模型的建立和应用[J].中华实验外科杂志,2008,25(2):270-272.