IAA对老芒麦幼苗生理及生长的影响
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摘要
以3份不同来源老芒麦(Elymus sibiricus)(E01、E02、E03)为材料,采用营养液砂培法,研究不同浓度吲哚乙酸(IAA)(0、10、20、30、40 mg/L)处理对老芒麦幼苗生理及生长的影响。结果表明:(1)IAA诱导了老芒麦幼苗根系的生长。不同浓度IAA处理下,总根长表现为20 mg/L IAA处理较好;30 mg/L IAA处理对根直径和表面积有显著促进作用,尤其对天祝老芒麦(E03)促进效果明显,其直径较对照增加了71.4%;(2)30 mg/L IAA处理下,3份老芒麦株高,叶绿素含量及POD活性表现最佳;天祝老芒麦株高较对照增加了49.0%(P<0.05),四川老芒麦(E02)POD活性较对照增加了80.5%(P<0.05)。20 mg/L IAA处理对SOD活性和可溶性蛋白含量有明显促进作用,四川老芒麦SOD活性较对照增加了70%(P<0.05)。综合比较表明,30 mg/L IAA对天祝老芒麦幼苗促生效果较好。
To investigate the effects of IAA on the growth and physiological characteristics of Elymus sibiricuss seedings,three E.sibiricus(E01、E02、E03)from different sources were used as materials by the sand cultivate with nutrient solutions.The results showed that: 1) IAA induced the root system′growth of E.sibiricuss seedlings.The treatment of 20 mg/L IAA(I2) had a significant effect on the length of total root;and the treatment of 30 mg/L IAA(I3) had a significant promoting effect on the indexes of root diameter and root surface area,the root diameter of Tianzhu E.sibiricuss(E03) increased by 71.4% compared with the control,2) The contents of plant height,chlorophyll and POD activity had a high value under the treatment of 30 mg/L IAA;especially the plant height of Tianzhu Elymus sibiricuss increased by 49.0% and the POD activity of Sichuan E.sibiricuss(E02)increased by 80.5% compared with the control,Respectively.The treatment of 20 mg/L IAA had a significant effect on the content of SOD activity and soluble protein;the SOD activity of Sichuan E.sibiricuss increased by 70% compared with the control.Comprehensive comparison showed that 30 mg/L IAA had a good effect on growth of Tianzhu E.sibiricuss.
To investigate the effects of IAA on the growth and physiological characteristics of Elymus sibiricuss seedings,three E.sibiricus(E01、E02、E03)from different sources were used as materials by the sand cultivate with nutrient solutions.The results showed that: 1) IAA induced the root system′growth of E.sibiricuss seedlings.The treatment of 20 mg/L IAA(I2) had a significant effect on the length of total root;and the treatment of 30 mg/L IAA(I3) had a significant promoting effect on the indexes of root diameter and root surface area,the root diameter of Tianzhu E.sibiricuss(E03) increased by 71.4% compared with the control,2) The contents of plant height,chlorophyll and POD activity had a high value under the treatment of 30 mg/L IAA;especially the plant height of Tianzhu Elymus sibiricuss increased by 49.0% and the POD activity of Sichuan E.sibiricuss(E02)increased by 80.5% compared with the control,Respectively.The treatment of 20 mg/L IAA had a significant effect on the content of SOD activity and soluble protein;the SOD activity of Sichuan E.sibiricuss increased by 70% compared with the control.Comprehensive comparison showed that 30 mg/L IAA had a good effect on growth of Tianzhu E.sibiricuss.
引文
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[23] 李千惠,范俊俊,赵明明,等.外源激素在金雀花扦插生根进程中的调节机制[J].中南林业科技大学学报,2017,37(10):54-60.
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[26] 卿纯,张卉灵,林毅青,等.生长素吲哚乙酸对铜绿微囊藻生理生化及产毒特性的影响[J].水生生物学报,2018 (4):832-838.
[27] 王岚,杨敏,黄芙蓉,等.不同浓度IAA对七叶一枝花生理特性的影响[J].安徽农学通报,2014(22):46-47.
[28] 孔德政,申雪莹,孟伟芳,等.外源激素对碗莲开花及酶活性的影响[J].东北林业大学学报,2015,43(3):79-82.
[29] 王宏信,杨素丹,刘红梅.不同植物生长调节剂对金荞麦种子萌发及幼苗生长的影响[J].种子,2017,36(5):19-22.
[30] 朱金方,刘京涛,陆兆华,等.盐胁迫对中国柽柳幼苗生理特性的影响[J].生态学报,2015,35(15):5140-5146.
[31] Sheokand S,Kumari A,Sawhney V.Effect of nitric oxide and putrescine on antioxidative responses under NaCl stress in chickpea plants[J].Physiology & Molecular Biology of Plants,2008,14(4):355-362.
[32] 邹竣竹.激素浸种对野牛草种子发芽与幼苗生长生理的影响[D].北京:中国林业科学研究院,2017.
[2] 陈利军,邹邦基,史奕,等.旱地施肥对春小麦根系生长、代谢的影响及促进水分有效利用的机理[J].植物营养与肥料学报,1995,2(2):26-32.
[3] Zhang W,Fan J,Tan Q,et al.Mechanisms Underlying the Regulation of Root Formation in Malus hupehensis,Stem Cuttings by Using Exogenous Hormones[J].Journal of Plant Growth Regulation,2017,36(1):174-185.
[4] 武维华.植物生理学[M].北京:科学出版社,2008.
[5] 邹竣竹,韩蕾,李德颖,等.赤霉素和生长素浸种对野牛草种子萌发及幼苗生长生理的影响[J].草业科学,2017,34(9):1838-1846.
[6] 李鹏程,董合林,刘爱忠,等.外源激素对棉花前期生长发育的影响[J].棉花学报,2013,25(3):278-282.
[7] 陈海生.外源IAA对杂交稻生育后期叶片生理特性的影响[J].河南农业科学,2012,41(5):31-32.
[8] Metaxas D J,Syros T D,Yupsanis T,Economou AS.Peroxidases during adventitious rooting in cuttings of Arbutus unedo and Taxus baccata as affected by plant genotype and growth regulator treatment[J].Plant Growth Regul,2004;44(3):257-266.
[9] 曹颖,胡尚连,卢学琴,等.GA3和IAA对慈竹可溶性蛋白质含量动态积累的调控[J].安徽农业科学,2008,36(17):7093-7094.
[10] 陈默君,贾慎修.中国饲用植物[M].北京:中国农业出版社,121-122.
[11] 陈秉龙.环青海湖地区四个老芒麦品种比较试验[J].青海草业,2017,26(3):19-21.
[12] 刘彩红,李成云,朴光一,等.IAA和NAA对不同羊草种子发芽和幼苗生长的影响[J].黑龙江畜牧兽医,2011(9):79-81.
[13] 陈超,赵丽丽,任登鸿,等.不同营养液对香根草生长的影响[J].贵州农业科学,2014(5):86-88.
[14] 潘静,韩蕾.葡萄叶片叶绿素含量测定方法比较[J].西北园艺,2017(6):58-60.
[15] 邹琦.植物生理学实验指导[M].北京:高等教育出版社,2003.
[16] 李合生.植物生理生化原理和技术[M].北京:高等教育出版社,2000.
[17] 李文明,徐永艳,胡平.不同浓度IAA对香叶树扦插生根的影响[J].西部林业科学,2013,42(2):78-81.
[18] 孙同高.植物生长调节剂对土沉香和铁冬青苗木生理与根系生长的影响[D].广州:华南农业大学,2016.
[19] 徐长贵,张重叶,金子煜,等.吲哚乙酸对五叶地锦扦插生根的影响[J].中国农业科技导报,2017,19(3):26-30.
[20] 尤桂春,武竞超,林文忠.IAA不同浓度处理对柠檬枝条扦插生根的影响[J].东南园艺,2016,4(1):8-9.
[21] 周索,杜丽.四种生长素对拟南芥根生长发育的影响[J].北方园艺,2008(3):51-52.
[22] 王媛媛,李永霞,马和平,等.IAA不同处理方法对西藏不同地区银白杨扦插生根及生长的影响[J].高原农业,2018,2(4):419-426.
[23] 李千惠,范俊俊,赵明明,等.外源激素在金雀花扦插生根进程中的调节机制[J].中南林业科技大学学报,2017,37(10):54-60.
[24] 王昱淇,王有科.外源激素对沙生植物梭梭生根及生长的影响[J].现代农业科技,2014(16):148-149.
[25] 魏卫东,李希来,田丰,等.外源激素对丸粒化披碱草种子萌发及幼苗生长的影响[J].中国种业,2010(8):60-62.
[26] 卿纯,张卉灵,林毅青,等.生长素吲哚乙酸对铜绿微囊藻生理生化及产毒特性的影响[J].水生生物学报,2018 (4):832-838.
[27] 王岚,杨敏,黄芙蓉,等.不同浓度IAA对七叶一枝花生理特性的影响[J].安徽农学通报,2014(22):46-47.
[28] 孔德政,申雪莹,孟伟芳,等.外源激素对碗莲开花及酶活性的影响[J].东北林业大学学报,2015,43(3):79-82.
[29] 王宏信,杨素丹,刘红梅.不同植物生长调节剂对金荞麦种子萌发及幼苗生长的影响[J].种子,2017,36(5):19-22.
[30] 朱金方,刘京涛,陆兆华,等.盐胁迫对中国柽柳幼苗生理特性的影响[J].生态学报,2015,35(15):5140-5146.
[31] Sheokand S,Kumari A,Sawhney V.Effect of nitric oxide and putrescine on antioxidative responses under NaCl stress in chickpea plants[J].Physiology & Molecular Biology of Plants,2008,14(4):355-362.
[32] 邹竣竹.激素浸种对野牛草种子发芽与幼苗生长生理的影响[D].北京:中国林业科学研究院,2017.