地衣芽孢杆菌MYS68的鉴定及发酵培养基优化
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摘要
对筛选出的MYS68菌株进行了16SrDNA序列分析鉴定,确定该菌为地衣芽孢杆菌。为了得到地衣芽孢杆菌MYS68发酵培养基的最佳配方组分,试验采用单因素试验和正交试验优化其摇瓶发酵培养基。结果表明,碳源和氮源对地衣芽孢杆菌发酵影响的显著性次序为:玉米粉>豆饼粉>蛋白胨>酵母膏。发酵培养基的最佳组分和配比为:玉米粉0.5%,蛋白胨1.0%,豆饼粉0.3%,酵母膏0.5%,硫酸锰0.001%,磷酸二氢钾0.1%,硫酸镁0.02%,起始pH值为7.5。此条件下发酵液的菌体浓度可达2.11×109 CFU/ml,经检测芽孢率为96%。说明地衣芽胞杆菌发酵培养基的最佳配比可在一定程度上提高地衣芽孢杆菌发酵液的菌体浓度和芽孢率。
A 16 SrDNA sequence analysis was performed for the screened MYS68 strains,and the bacterium was identified as bacillus licheniformis.In order to obtain the optimal composition of MYS68 fermentation medium,single factor and orthogonal test were adopted to optimize the fermentation medium.The results showed that the primary and secondary sequence of the parametersthat carbon source and nitrogen source affected the fermentation of bacillus lichenium was corn flour,bean cake powder,peptone,yeast extract.The optimal composition of fermentation medium was as follows:corn 0.5%,peptone 1.0%,bean cake powder 0.3%,yeast extract 0.5%,manganese sulfate 0.001%,potassium dihydrogen phosphate 0.1%,magnesium sulfate 0.02%,initial pH value 7.5.Under this condition,the bacterial concentration of the fermentation medium reached 2.11× 109 CFU/ml,and the spore rate was 96%.It is indicated that the optimal composition of bacillus licheniformis MYS68 fermentation medium can increase the bacterial concentration and the spore rate partly.
A 16 SrDNA sequence analysis was performed for the screened MYS68 strains,and the bacterium was identified as bacillus licheniformis.In order to obtain the optimal composition of MYS68 fermentation medium,single factor and orthogonal test were adopted to optimize the fermentation medium.The results showed that the primary and secondary sequence of the parametersthat carbon source and nitrogen source affected the fermentation of bacillus lichenium was corn flour,bean cake powder,peptone,yeast extract.The optimal composition of fermentation medium was as follows:corn 0.5%,peptone 1.0%,bean cake powder 0.3%,yeast extract 0.5%,manganese sulfate 0.001%,potassium dihydrogen phosphate 0.1%,magnesium sulfate 0.02%,initial pH value 7.5.Under this condition,the bacterial concentration of the fermentation medium reached 2.11× 109 CFU/ml,and the spore rate was 96%.It is indicated that the optimal composition of bacillus licheniformis MYS68 fermentation medium can increase the bacterial concentration and the spore rate partly.
引文
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[3]付维来,杜建涛,刘鹏,等.地衣芽孢杆菌M109高密度发酵条件的优化[J].中国畜牧兽医,2012,(11):215-219.
[4]杨阳,张付云,苍桂璐.地衣芽孢杆菌生物活性物质应用研究进展[J].生物技术进展,2013,3(1):22-26.
[5]朱利平,黄惠莉.产壳聚糖酶海洋真菌鉴定、寡营养培养基优化及廉价培养基研究[J].黑龙江畜牧兽医,2011,37(5):35-39.
[6]纪明山,王毅婧.地衣芽孢杆菌生防菌株SDYT-79发酵条件优化[J].沈阳农业大学学报,2011(2):164-169.
[7]张红艳,岳淑宁,张强,等.地衣芽孢杆菌在饲料工业中的应用及研究[J].畜牧与饲料科学,2017,38(231):26-28.