一株产壳聚糖酶海洋真菌MF-08的分离鉴定及酶学性质研究
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摘要
从实验室保存的海洋产壳聚糖酶真菌中分离纯化出一株产壳聚糖酶活性较高的菌株,命名为MF-08。通过26S r DNA序列分析并结合形态学鉴定,将海洋真菌MF-08归为黄曲霉群。壳聚糖酶的最适作用温度为40℃;最适反应p H为5.2;酶活在p H4.0~6.0和小于40℃范围内相对稳定;Cu2+、Fe3+和Hg2+对壳聚糖酶活性有明显的抑制作用。
Isolated and purified a high enzyme activity strain from the chitosanase producing Marine fungi which preservation in laboratory,it was named MF-08. Based on the 26 S r DNA sequence analysis and morphological properties,Marine fungi MF-08 was assessed to be Aspergillus flavus. The optimum temperature of chitosanase was 40 ℃,the optimum p H was 5.2,the chitosanase was stable below 40 ℃ and p H4.0~6.0,respectively. The enzyme activity was inhibited by Cu2+,Fe3+and Hg2+.
Isolated and purified a high enzyme activity strain from the chitosanase producing Marine fungi which preservation in laboratory,it was named MF-08. Based on the 26 S r DNA sequence analysis and morphological properties,Marine fungi MF-08 was assessed to be Aspergillus flavus. The optimum temperature of chitosanase was 40 ℃,the optimum p H was 5.2,the chitosanase was stable below 40 ℃ and p H4.0~6.0,respectively. The enzyme activity was inhibited by Cu2+,Fe3+and Hg2+.
引文
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[13]杨立红,程仕伟,冯志彬,等.链霉菌壳聚糖酶的纯化及其酶学性质[J].生物加工过程,2013,11(3):52-58.
[14]Anh Dzung Nguyen,Chun-Ching Huang,Tzu-Wen Liang.Production and purification of a fungal chitosanase and chitooligomers from Penicilliumjanthinellum D4 and discovery of the enzyme activators[J].Carbohydrate Polymers:Scientific and Technological Aspects of Industrially Important Polysaccharides,2014,108:331-337.
[2]朱利平,黄惠莉.产壳聚糖酶海洋真菌鉴定、寡营养培养基优化及廉价培养基研究[J].食品与发酵工业,2011,37(5):35-39.
[3]王艳君,廖春林.产壳聚糖酶菌株的单因素及复合条件诱变育种[J].河南城建学院学报,2012,21(4):24-31.
[4]宋晓晨.海洋微生物壳聚糖酶菌株筛选及培养条件[D].大连:大连工业大学,2010.
[5]李风平,何潇,鲍晓明.壳聚糖酶产生菌的筛选及酶解产物的初步研究[J].山东大学学报,2003,38(1):96-102.
[6]袁道强,黄建华.生物化学实验和技术[M].北京:中国轻工业出版社,2006.
[7]罗雪云,刘宏道,周桂莲,等.食品卫生微生物检验标准手册[M].北京:中国标准出版社,1995:243.
[8]Katsuaki Hirano,Sitthinan Arayaveerasid,Kiyohiko Seki.Characterization of a Chitosanase from Aspergillus fumigatus ATCC13073[J].Bioscience,Biotechnology,and Biochemistry,2012,76(8):1523-1528.
[9]王艳君,卓少玲,陈盛,等.产壳聚糖酶菌株的筛选、鉴定及酶学特性分析[J].微生物学通报,2012,39(12):1734-1745.
[10]阎贺静,周念波,涂邵勇,等.壳聚糖酶生产菌筛选、鉴定及其酶学性质[J].广东农业科学,2012(24):161-164.
[11]袁建平,侯晓强.壳聚糖酶的分离纯化及性质研究[J].湖北农业科学,2013,52(15):3647-3649.
[12]赵玉萍,钱红梅.绿色木霉产壳聚糖酶的分离纯化及酶学性质[J].中国粮油学报,2009,24(4):144-147.
[13]杨立红,程仕伟,冯志彬,等.链霉菌壳聚糖酶的纯化及其酶学性质[J].生物加工过程,2013,11(3):52-58.
[14]Anh Dzung Nguyen,Chun-Ching Huang,Tzu-Wen Liang.Production and purification of a fungal chitosanase and chitooligomers from Penicilliumjanthinellum D4 and discovery of the enzyme activators[J].Carbohydrate Polymers:Scientific and Technological Aspects of Industrially Important Polysaccharides,2014,108:331-337.